Development of a peptide-based immunochromatographic strip for differentiation of serotype O Foot-and-mouth disease virus–infected pigs from vaccinated pigs
An immunochromatographic strip for discriminating Foot-and-mouth disease virus (FMDV) infected from vaccinated pigs was developed based on synthetic peptide. Five peptides designed from the amino acid sequences of nonstructural proteins (NSP) of FMDV were synthesized, and pep5 located in NSP 3B reac...
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| Veröffentlicht in: | Journal of veterinary diagnostic investigation 2010-05, Vol.22 (3), p.412-415 |
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| container_title | Journal of veterinary diagnostic investigation |
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| creator | Yang, Suzhen Yang, Jifei Zhang, Gaiping Qiao, Songlin Wang, Xuannian Zhao, Dong Li, Xuewu Deng, Ruiguang Zhi, Aimin You, Leiming Chai, Sujun Teng, Man |
| description | An immunochromatographic strip for discriminating Foot-and-mouth disease virus (FMDV) infected from vaccinated pigs was developed based on synthetic peptide. Five peptides designed from the amino acid sequences of nonstructural proteins (NSP) of FMDV were synthesized, and pep5 located in NSP 3B reacted strongly with serum from FMDV-infected pigs but did not react with serum samples from healthy vaccinated pigs. An immunochromatographic strip was developed by using colloidal gold labeled with pep5 as the detector. Staphylococcal protein A and rabbit against peptide-conjugated ovalbumin antibody immunoglobulin G were blotted on the nitrocellulose membrane for the test and control lines. In comparison with 2 commercial NSP enzyme-linked immunosorbent assays, the peptide-based strip showed good specificity and sensitivity. The apparent agreements of this new assay with Ceditest® ELISA and UBI® ELISA were 98.59% and 96.63%, respectively. These results indicate that the strip can be adequately used to discriminate FMDV-infected animals from vaccinated animals. |
| doi_str_mv | 10.1177/104063871002200311 |
| format | Article |
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Five peptides designed from the amino acid sequences of nonstructural proteins (NSP) of FMDV were synthesized, and pep5 located in NSP 3B reacted strongly with serum from FMDV-infected pigs but did not react with serum samples from healthy vaccinated pigs. An immunochromatographic strip was developed by using colloidal gold labeled with pep5 as the detector. Staphylococcal protein A and rabbit against peptide-conjugated ovalbumin antibody immunoglobulin G were blotted on the nitrocellulose membrane for the test and control lines. In comparison with 2 commercial NSP enzyme-linked immunosorbent assays, the peptide-based strip showed good specificity and sensitivity. The apparent agreements of this new assay with Ceditest® ELISA and UBI® ELISA were 98.59% and 96.63%, respectively. These results indicate that the strip can be adequately used to discriminate FMDV-infected animals from vaccinated animals.</description><identifier>ISSN: 1040-6387</identifier><identifier>EISSN: 1943-4936</identifier><identifier>DOI: 10.1177/104063871002200311</identifier><identifier>PMID: 20453216</identifier><language>eng</language><publisher>Los Angeles, CA: SAGE Publications</publisher><subject>accuracy ; Amino Acid Sequence ; amino acids ; Animals ; bioassays ; Carrier State - veterinary ; Cattle ; Cattle Diseases - immunology ; Cattle Diseases - virology ; disease detection ; Enzyme-Linked Immunosorbent Assay - methods ; Enzyme-Linked Immunosorbent Assay - veterinary ; foot-and-mouth disease ; Foot-and-Mouth Disease - immunology ; Foot-and-Mouth Disease - prevention & control ; Foot-and-Mouth Disease - transmission ; Foot-and-Mouth Disease - virology ; Foot-and-mouth disease virus ; Foot-and-Mouth Disease Virus - genetics ; Foot-and-Mouth Disease Virus - immunology ; Goat Diseases - immunology ; Goat Diseases - virology ; Goats ; immunoassays ; immunochromatographic strip ; Immunoglobulin G - immunology ; laboratory techniques ; Molecular Sequence Data ; Ovalbumin - immunology ; pathogen identification ; pathotypes ; Peptide Fragments - chemistry ; Peptide Fragments - immunology ; Rabbits ; Sensitivity and Specificity ; serotypes ; Swine ; swine diseases ; Swine Diseases - immunology ; Swine Diseases - virology ; synthetic peptides ; vaccination ; Vaccination - methods ; Vaccination - veterinary ; vaccines ; validity ; Viral Nonstructural Proteins - chemistry ; Viral Nonstructural Proteins - genetics ; Viral Nonstructural Proteins - immunology ; viral proteins ; Viral Vaccines - immunology</subject><ispartof>Journal of veterinary diagnostic investigation, 2010-05, Vol.22 (3), p.412-415</ispartof><rights>2010 American Association of Veterinary Laboratory Diagnosticians</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c410t-14d9bf5bbe58be057cda33e1cf346826abee99e8805a894ae6578ece2742881a3</citedby><cites>FETCH-LOGICAL-c410t-14d9bf5bbe58be057cda33e1cf346826abee99e8805a894ae6578ece2742881a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/104063871002200311$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/104063871002200311$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,780,784,21819,27924,27925,43621,43622</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20453216$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yang, Suzhen</creatorcontrib><creatorcontrib>Yang, Jifei</creatorcontrib><creatorcontrib>Zhang, Gaiping</creatorcontrib><creatorcontrib>Qiao, Songlin</creatorcontrib><creatorcontrib>Wang, Xuannian</creatorcontrib><creatorcontrib>Zhao, Dong</creatorcontrib><creatorcontrib>Li, Xuewu</creatorcontrib><creatorcontrib>Deng, Ruiguang</creatorcontrib><creatorcontrib>Zhi, Aimin</creatorcontrib><creatorcontrib>You, Leiming</creatorcontrib><creatorcontrib>Chai, Sujun</creatorcontrib><creatorcontrib>Teng, Man</creatorcontrib><title>Development of a peptide-based immunochromatographic strip for differentiation of serotype O Foot-and-mouth disease virus–infected pigs from vaccinated pigs</title><title>Journal of veterinary diagnostic investigation</title><addtitle>J Vet Diagn Invest</addtitle><description>An immunochromatographic strip for discriminating Foot-and-mouth disease virus (FMDV) infected from vaccinated pigs was developed based on synthetic peptide. Five peptides designed from the amino acid sequences of nonstructural proteins (NSP) of FMDV were synthesized, and pep5 located in NSP 3B reacted strongly with serum from FMDV-infected pigs but did not react with serum samples from healthy vaccinated pigs. An immunochromatographic strip was developed by using colloidal gold labeled with pep5 as the detector. Staphylococcal protein A and rabbit against peptide-conjugated ovalbumin antibody immunoglobulin G were blotted on the nitrocellulose membrane for the test and control lines. In comparison with 2 commercial NSP enzyme-linked immunosorbent assays, the peptide-based strip showed good specificity and sensitivity. The apparent agreements of this new assay with Ceditest® ELISA and UBI® ELISA were 98.59% and 96.63%, respectively. These results indicate that the strip can be adequately used to discriminate FMDV-infected animals from vaccinated animals.</description><subject>accuracy</subject><subject>Amino Acid Sequence</subject><subject>amino acids</subject><subject>Animals</subject><subject>bioassays</subject><subject>Carrier State - veterinary</subject><subject>Cattle</subject><subject>Cattle Diseases - immunology</subject><subject>Cattle Diseases - virology</subject><subject>disease detection</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Enzyme-Linked Immunosorbent Assay - veterinary</subject><subject>foot-and-mouth disease</subject><subject>Foot-and-Mouth Disease - immunology</subject><subject>Foot-and-Mouth Disease - prevention & control</subject><subject>Foot-and-Mouth Disease - transmission</subject><subject>Foot-and-Mouth Disease - virology</subject><subject>Foot-and-mouth disease virus</subject><subject>Foot-and-Mouth Disease Virus - genetics</subject><subject>Foot-and-Mouth Disease Virus - immunology</subject><subject>Goat Diseases - immunology</subject><subject>Goat Diseases - virology</subject><subject>Goats</subject><subject>immunoassays</subject><subject>immunochromatographic strip</subject><subject>Immunoglobulin G - immunology</subject><subject>laboratory techniques</subject><subject>Molecular Sequence Data</subject><subject>Ovalbumin - immunology</subject><subject>pathogen identification</subject><subject>pathotypes</subject><subject>Peptide Fragments - chemistry</subject><subject>Peptide Fragments - immunology</subject><subject>Rabbits</subject><subject>Sensitivity and Specificity</subject><subject>serotypes</subject><subject>Swine</subject><subject>swine diseases</subject><subject>Swine Diseases - immunology</subject><subject>Swine Diseases - virology</subject><subject>synthetic peptides</subject><subject>vaccination</subject><subject>Vaccination - methods</subject><subject>Vaccination - veterinary</subject><subject>vaccines</subject><subject>validity</subject><subject>Viral Nonstructural Proteins - chemistry</subject><subject>Viral Nonstructural Proteins - genetics</subject><subject>Viral Nonstructural Proteins - immunology</subject><subject>viral proteins</subject><subject>Viral Vaccines - immunology</subject><issn>1040-6387</issn><issn>1943-4936</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kb1uFDEURi0EIiHwAhTgjmqIf8YznhIlBJAipYDU1h3P9a6jnbGxPSul4x3oeTieBK82oUGiutbV-c6V9RHymrP3nPf9OWct66TuOWNCMCY5f0JO-dDKph1k97S-K9AciBPyIuc7xpRQPX9OTgRrlRS8OyW_LnGPuxBnXAoNjgKNGIufsBkh40T9PK9LsNsUZihhkyBuvaW5JB-pC4lO3jlMNeyh-LAcFBlTKPcR6Q29CqE0sEzNHNayrXDGaqV7n9b8-8dPvzi0pV6JfpOpqzfoHqz1CzwuX5JnDnYZXz3MM3J79fHbxefm-ubTl4sP141tOSsNb6dhdGocUekRmertBFIit062nRYdjIjDgFozBXpoATvVa7Qo-lZozUGekXdHb0zh-4q5mNlni7sdLBjWbHophWQd7yopjqRNIeeEzsTkZ0j3hjNzqMX8W0sNvXnQr-OM09_IYw8VOD8CGTZo7sKalvrd_yvfHhMOgoFN8tncfhWMS8a1qpCSfwAcK6Mz</recordid><startdate>20100501</startdate><enddate>20100501</enddate><creator>Yang, Suzhen</creator><creator>Yang, Jifei</creator><creator>Zhang, Gaiping</creator><creator>Qiao, Songlin</creator><creator>Wang, Xuannian</creator><creator>Zhao, Dong</creator><creator>Li, Xuewu</creator><creator>Deng, Ruiguang</creator><creator>Zhi, Aimin</creator><creator>You, Leiming</creator><creator>Chai, Sujun</creator><creator>Teng, Man</creator><general>SAGE Publications</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20100501</creationdate><title>Development of a peptide-based immunochromatographic strip for differentiation of serotype O Foot-and-mouth disease virus–infected pigs from vaccinated pigs</title><author>Yang, Suzhen ; Yang, Jifei ; Zhang, Gaiping ; Qiao, Songlin ; Wang, Xuannian ; Zhao, Dong ; Li, Xuewu ; Deng, Ruiguang ; Zhi, Aimin ; You, Leiming ; Chai, Sujun ; Teng, Man</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c410t-14d9bf5bbe58be057cda33e1cf346826abee99e8805a894ae6578ece2742881a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>accuracy</topic><topic>Amino Acid Sequence</topic><topic>amino acids</topic><topic>Animals</topic><topic>bioassays</topic><topic>Carrier State - veterinary</topic><topic>Cattle</topic><topic>Cattle Diseases - immunology</topic><topic>Cattle Diseases - virology</topic><topic>disease detection</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Enzyme-Linked Immunosorbent Assay - veterinary</topic><topic>foot-and-mouth disease</topic><topic>Foot-and-Mouth Disease - immunology</topic><topic>Foot-and-Mouth Disease - prevention & control</topic><topic>Foot-and-Mouth Disease - transmission</topic><topic>Foot-and-Mouth Disease - virology</topic><topic>Foot-and-mouth disease virus</topic><topic>Foot-and-Mouth Disease Virus - genetics</topic><topic>Foot-and-Mouth Disease Virus - immunology</topic><topic>Goat Diseases - immunology</topic><topic>Goat Diseases - virology</topic><topic>Goats</topic><topic>immunoassays</topic><topic>immunochromatographic strip</topic><topic>Immunoglobulin G - immunology</topic><topic>laboratory techniques</topic><topic>Molecular Sequence Data</topic><topic>Ovalbumin - immunology</topic><topic>pathogen identification</topic><topic>pathotypes</topic><topic>Peptide Fragments - chemistry</topic><topic>Peptide Fragments - immunology</topic><topic>Rabbits</topic><topic>Sensitivity and Specificity</topic><topic>serotypes</topic><topic>Swine</topic><topic>swine diseases</topic><topic>Swine Diseases - immunology</topic><topic>Swine Diseases - virology</topic><topic>synthetic peptides</topic><topic>vaccination</topic><topic>Vaccination - methods</topic><topic>Vaccination - veterinary</topic><topic>vaccines</topic><topic>validity</topic><topic>Viral Nonstructural Proteins - chemistry</topic><topic>Viral Nonstructural Proteins - genetics</topic><topic>Viral Nonstructural Proteins - immunology</topic><topic>viral proteins</topic><topic>Viral Vaccines - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yang, Suzhen</creatorcontrib><creatorcontrib>Yang, Jifei</creatorcontrib><creatorcontrib>Zhang, Gaiping</creatorcontrib><creatorcontrib>Qiao, Songlin</creatorcontrib><creatorcontrib>Wang, Xuannian</creatorcontrib><creatorcontrib>Zhao, Dong</creatorcontrib><creatorcontrib>Li, Xuewu</creatorcontrib><creatorcontrib>Deng, Ruiguang</creatorcontrib><creatorcontrib>Zhi, Aimin</creatorcontrib><creatorcontrib>You, Leiming</creatorcontrib><creatorcontrib>Chai, Sujun</creatorcontrib><creatorcontrib>Teng, Man</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of veterinary diagnostic investigation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yang, Suzhen</au><au>Yang, Jifei</au><au>Zhang, Gaiping</au><au>Qiao, Songlin</au><au>Wang, Xuannian</au><au>Zhao, Dong</au><au>Li, Xuewu</au><au>Deng, Ruiguang</au><au>Zhi, Aimin</au><au>You, Leiming</au><au>Chai, Sujun</au><au>Teng, Man</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of a peptide-based immunochromatographic strip for differentiation of serotype O Foot-and-mouth disease virus–infected pigs from vaccinated pigs</atitle><jtitle>Journal of veterinary diagnostic investigation</jtitle><addtitle>J Vet Diagn Invest</addtitle><date>2010-05-01</date><risdate>2010</risdate><volume>22</volume><issue>3</issue><spage>412</spage><epage>415</epage><pages>412-415</pages><issn>1040-6387</issn><eissn>1943-4936</eissn><abstract>An immunochromatographic strip for discriminating Foot-and-mouth disease virus (FMDV) infected from vaccinated pigs was developed based on synthetic peptide. Five peptides designed from the amino acid sequences of nonstructural proteins (NSP) of FMDV were synthesized, and pep5 located in NSP 3B reacted strongly with serum from FMDV-infected pigs but did not react with serum samples from healthy vaccinated pigs. An immunochromatographic strip was developed by using colloidal gold labeled with pep5 as the detector. Staphylococcal protein A and rabbit against peptide-conjugated ovalbumin antibody immunoglobulin G were blotted on the nitrocellulose membrane for the test and control lines. In comparison with 2 commercial NSP enzyme-linked immunosorbent assays, the peptide-based strip showed good specificity and sensitivity. The apparent agreements of this new assay with Ceditest® ELISA and UBI® ELISA were 98.59% and 96.63%, respectively. These results indicate that the strip can be adequately used to discriminate FMDV-infected animals from vaccinated animals.</abstract><cop>Los Angeles, CA</cop><pub>SAGE Publications</pub><pmid>20453216</pmid><doi>10.1177/104063871002200311</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | accuracy Amino Acid Sequence amino acids Animals bioassays Carrier State - veterinary Cattle Cattle Diseases - immunology Cattle Diseases - virology disease detection Enzyme-Linked Immunosorbent Assay - methods Enzyme-Linked Immunosorbent Assay - veterinary foot-and-mouth disease Foot-and-Mouth Disease - immunology Foot-and-Mouth Disease - prevention & control Foot-and-Mouth Disease - transmission Foot-and-Mouth Disease - virology Foot-and-mouth disease virus Foot-and-Mouth Disease Virus - genetics Foot-and-Mouth Disease Virus - immunology Goat Diseases - immunology Goat Diseases - virology Goats immunoassays immunochromatographic strip Immunoglobulin G - immunology laboratory techniques Molecular Sequence Data Ovalbumin - immunology pathogen identification pathotypes Peptide Fragments - chemistry Peptide Fragments - immunology Rabbits Sensitivity and Specificity serotypes Swine swine diseases Swine Diseases - immunology Swine Diseases - virology synthetic peptides vaccination Vaccination - methods Vaccination - veterinary vaccines validity Viral Nonstructural Proteins - chemistry Viral Nonstructural Proteins - genetics Viral Nonstructural Proteins - immunology viral proteins Viral Vaccines - immunology |
| title | Development of a peptide-based immunochromatographic strip for differentiation of serotype O Foot-and-mouth disease virus–infected pigs from vaccinated pigs |
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