A steady-state kinetic method for the verification of the rapid-equilibrium assumption in allosteric enzymes

A method for testing the validity of the rapid-equilibrium assumption as it might apply to allosteric enzymes using exclusively steady-state kinetic data is presented. The method is based upon a recognition that the ratio of apparent dissociation constants for the allosteric ligand, obtained under c...

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Veröffentlicht in:	Analytical biochemistry 1992-11, Vol.206 (2), p.394-399
Hauptverfasser:	Symcox, Marina M., Reinhart, Gregory D.
Format:	Artikel
Sprache:	eng
Schlagworte:	Allosteric Regulation Analytical, structural and metabolic biochemistry Biological and medical sciences Enzymes - metabolism Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology General aspects, investigation methods Isocitrate Dehydrogenase - metabolism Kinetics Mathematics Models, Theoretical Thermodynamics
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