Application of Whole Mouse Embryo Culture System on Stem Cell Research
The normal development of mouse embryo in vivo could be maintained in vitro up to 72 h in the presence of rat serum which is continuously supplied with the appropriate concentration of O 2 and CO 2 . There are several applications of the whole mouse embryo culture model for study of cellular dynamic...
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| Veröffentlicht in: | Stem cell reviews 2009-06, Vol.5 (2), p.175-180 |
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| creator | Kulkeaw, Kasem Mizuochi, Chiyo Horio, Yuka Osumi, Noriko Tsuji, Kohichiro Sugiyama, Daisuke |
| description | The normal development of mouse embryo
in vivo
could be maintained
in vitro
up to 72 h in the presence of rat serum which is continuously supplied with the appropriate concentration of O
2
and CO
2
. There are several applications of the whole mouse embryo culture model for study of cellular dynamics in hematopoiesis and its interaction with vasculogenesis. In this protocol, we have described details of manipulation techniques in combination with the whole embryo culture and also some advance techniques applied to the mouse embryo such as intra-cardiac inoculation of acetylated low density lipoprotein for cell-specific labeling and engraftment of donor yolk-sac from different genotype/phenotype mouse embryo onto the yolk-sac of host mouse for study of the dynamic distribution of hematopoietic cell. |
| doi_str_mv | 10.1007/s12015-009-9064-2 |
| format | Article |
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in vivo
could be maintained
in vitro
up to 72 h in the presence of rat serum which is continuously supplied with the appropriate concentration of O
2
and CO
2
. There are several applications of the whole mouse embryo culture model for study of cellular dynamics in hematopoiesis and its interaction with vasculogenesis. In this protocol, we have described details of manipulation techniques in combination with the whole embryo culture and also some advance techniques applied to the mouse embryo such as intra-cardiac inoculation of acetylated low density lipoprotein for cell-specific labeling and engraftment of donor yolk-sac from different genotype/phenotype mouse embryo onto the yolk-sac of host mouse for study of the dynamic distribution of hematopoietic cell.</description><identifier>ISSN: 1550-8943</identifier><identifier>ISSN: 2629-3269</identifier><identifier>EISSN: 1558-6804</identifier><identifier>EISSN: 2629-3277</identifier><identifier>DOI: 10.1007/s12015-009-9064-2</identifier><identifier>PMID: 19521805</identifier><language>eng</language><publisher>New York: Humana Press Inc</publisher><subject>Animals ; Biomedical and Life Sciences ; Biomedical Engineering and Bioengineering ; Cell Biology ; Embryo Culture Techniques - instrumentation ; Embryo Culture Techniques - methods ; Embryo Research ; Life Sciences ; Low density lipoprotein ; Mice ; Mice, Inbred C57BL ; Protocols ; Regenerative Medicine/Tissue Engineering ; Stem Cells</subject><ispartof>Stem cell reviews, 2009-06, Vol.5 (2), p.175-180</ispartof><rights>Springer Science + Business Media 2009</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c436t-b09989c0f70ae09134d2eb119ac7e3b3aa69cdad08255e954ceac72b42b83d173</citedby><cites>FETCH-LOGICAL-c436t-b09989c0f70ae09134d2eb119ac7e3b3aa69cdad08255e954ceac72b42b83d173</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27929,27930</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19521805$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kulkeaw, Kasem</creatorcontrib><creatorcontrib>Mizuochi, Chiyo</creatorcontrib><creatorcontrib>Horio, Yuka</creatorcontrib><creatorcontrib>Osumi, Noriko</creatorcontrib><creatorcontrib>Tsuji, Kohichiro</creatorcontrib><creatorcontrib>Sugiyama, Daisuke</creatorcontrib><title>Application of Whole Mouse Embryo Culture System on Stem Cell Research</title><title>Stem cell reviews</title><addtitle>Stem Cell Rev and Rep</addtitle><addtitle>Stem Cell Rev Rep</addtitle><description>The normal development of mouse embryo
in vivo
could be maintained
in vitro
up to 72 h in the presence of rat serum which is continuously supplied with the appropriate concentration of O
2
and CO
2
. There are several applications of the whole mouse embryo culture model for study of cellular dynamics in hematopoiesis and its interaction with vasculogenesis. In this protocol, we have described details of manipulation techniques in combination with the whole embryo culture and also some advance techniques applied to the mouse embryo such as intra-cardiac inoculation of acetylated low density lipoprotein for cell-specific labeling and engraftment of donor yolk-sac from different genotype/phenotype mouse embryo onto the yolk-sac of host mouse for study of the dynamic distribution of hematopoietic cell.</description><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedical Engineering and Bioengineering</subject><subject>Cell Biology</subject><subject>Embryo Culture Techniques - instrumentation</subject><subject>Embryo Culture Techniques - methods</subject><subject>Embryo Research</subject><subject>Life Sciences</subject><subject>Low density lipoprotein</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Protocols</subject><subject>Regenerative Medicine/Tissue Engineering</subject><subject>Stem Cells</subject><issn>1550-8943</issn><issn>2629-3269</issn><issn>1558-6804</issn><issn>2629-3277</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kE1LAzEQhoMotlZ_gBdZvHhanXzsbnIspVWhIljFY8hmp7Zlv0x2D_33prZQEDxNYJ558_IQck3hngJkD54yoEkMoGIFqYjZCRnSJJFxKkGc_r4hlkrwAbnwfgPApZD0nAyoShiVkAzJbNy25dqabt3UUbOMPldNidFL03uMplXutk006cuudxgttr7DKgrcYjcnWJbRG3o0zq4uydnSlB6vDnNEPmbT98lTPH99fJ6M57EVPO3iHJSSysIyA4OgKBcFw5xSZWyGPOfGpMoWpgDJkgRVIiyGDcsFyyUvaMZH5G6f27rmu0ff6WrtbWhiagyddcY5Y1RQFcjbP-Sm6V0dymkpeaqEUCxAdA9Z13jvcKlbt66M22oKeqdY7xXroFjvFOvdzc0huM8rLI4XB6cBYHvAh1X9he748_-pP6XzhQg</recordid><startdate>20090601</startdate><enddate>20090601</enddate><creator>Kulkeaw, Kasem</creator><creator>Mizuochi, Chiyo</creator><creator>Horio, Yuka</creator><creator>Osumi, Noriko</creator><creator>Tsuji, Kohichiro</creator><creator>Sugiyama, Daisuke</creator><general>Humana Press Inc</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20090601</creationdate><title>Application of Whole Mouse Embryo Culture System on Stem Cell Research</title><author>Kulkeaw, Kasem ; 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in vivo
could be maintained
in vitro
up to 72 h in the presence of rat serum which is continuously supplied with the appropriate concentration of O
2
and CO
2
. There are several applications of the whole mouse embryo culture model for study of cellular dynamics in hematopoiesis and its interaction with vasculogenesis. In this protocol, we have described details of manipulation techniques in combination with the whole embryo culture and also some advance techniques applied to the mouse embryo such as intra-cardiac inoculation of acetylated low density lipoprotein for cell-specific labeling and engraftment of donor yolk-sac from different genotype/phenotype mouse embryo onto the yolk-sac of host mouse for study of the dynamic distribution of hematopoietic cell.</abstract><cop>New York</cop><pub>Humana Press Inc</pub><pmid>19521805</pmid><doi>10.1007/s12015-009-9064-2</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1550-8943 |
| ispartof | Stem cell reviews, 2009-06, Vol.5 (2), p.175-180 |
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| language | eng |
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| source | MEDLINE; Springer Online Journals Complete |
| subjects | Animals Biomedical and Life Sciences Biomedical Engineering and Bioengineering Cell Biology Embryo Culture Techniques - instrumentation Embryo Culture Techniques - methods Embryo Research Life Sciences Low density lipoprotein Mice Mice, Inbred C57BL Protocols Regenerative Medicine/Tissue Engineering Stem Cells |
| title | Application of Whole Mouse Embryo Culture System on Stem Cell Research |
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