Disruption and mapping of IDI1, the gene for isopentenyl diphosphate isomerase in Saccharomyces cerevisiae
Isopontenyl diphosphate isomerase catalyses an essential activation step in the isoprene biosynthetic pathwasy. The Saccharomyces cerevisiae gene for isomerase, IDI1, was recently isolated and characterized (Anderson et al. J. Biol. Chem. 1989aa, 264, 19169–19175). Wild‐type IDI1 was disrupted with...
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| Veröffentlicht in: | Yeast (Chichester, England) England), 1992-09, Vol.8 (9), p.743-748 |
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| creator | Mayer, Matthias P. Hahn, Frederick M. Stillman, David J. Poulter, C. Dale |
| description | Isopontenyl diphosphate isomerase catalyses an essential activation step in the isoprene biosynthetic pathwasy. The Saccharomyces cerevisiae gene for isomerase, IDI1, was recently isolated and characterized (Anderson et al. J. Biol. Chem. 1989aa, 264, 19169–19175). Wild‐type IDI1 was disrupted with a LEU2 marker, and the resulting DNA was used to transform a yeast leucine auxotroph. Southern blots of EcoRI fragments of chromosomal DNA from the diploid strain showed the expected fragments for intact and disrupted IDI1. Dissection and analysis of tetrads demonstrated that IDI1 is an essential single‐copy gene. A CHEF gel and clone grid filter analysis, followed by chromosomal mapping indicated that the gene is located on chromosome that the gene is located on the chromosome XVI approximately 55 kb contromere proximal to PEP4. |
| doi_str_mv | 10.1002/yea.320080907 |
| format | Article |
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A CHEF gel and clone grid filter analysis, followed by chromosomal mapping indicated that the gene is located on chromosome that the gene is located on the chromosome XVI approximately 55 kb contromere proximal to PEP4.</description><identifier>ISSN: 0749-503X</identifier><identifier>EISSN: 1097-0061</identifier><identifier>DOI: 10.1002/yea.320080907</identifier><identifier>PMID: 1441751</identifier><identifier>CODEN: YESTE3</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Biological and medical sciences ; Carbon-Carbon Double Bond Isomerases ; chromosome ; Chromosome Mapping ; DNA, Fungal - genetics ; Fundamental and applied biological sciences. Psychology ; Genes, Fungal ; Genes. 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Dale</creatorcontrib><title>Disruption and mapping of IDI1, the gene for isopentenyl diphosphate isomerase in Saccharomyces cerevisiae</title><title>Yeast (Chichester, England)</title><addtitle>Yeast</addtitle><description>Isopontenyl diphosphate isomerase catalyses an essential activation step in the isoprene biosynthetic pathwasy. The Saccharomyces cerevisiae gene for isomerase, IDI1, was recently isolated and characterized (Anderson et al. J. Biol. Chem. 1989aa, 264, 19169–19175). Wild‐type IDI1 was disrupted with a LEU2 marker, and the resulting DNA was used to transform a yeast leucine auxotroph. Southern blots of EcoRI fragments of chromosomal DNA from the diploid strain showed the expected fragments for intact and disrupted IDI1. Dissection and analysis of tetrads demonstrated that IDI1 is an essential single‐copy gene. A CHEF gel and clone grid filter analysis, followed by chromosomal mapping indicated that the gene is located on chromosome that the gene is located on the chromosome XVI approximately 55 kb contromere proximal to PEP4.</description><subject>Biological and medical sciences</subject><subject>Carbon-Carbon Double Bond Isomerases</subject><subject>chromosome</subject><subject>Chromosome Mapping</subject><subject>DNA, Fungal - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Fungal</subject><subject>Genes. Genome</subject><subject>Genetic Linkage</subject><subject>Isomerases - genetics</subject><subject>Isoprene</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Restriction Mapping</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - enzymology</subject><subject>Saccharomyces cerevisiae - genetics</subject><issn>0749-503X</issn><issn>1097-0061</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1P3DAQxa2Kii60R45IPiBOBGbsJI6PCGi7ElIPpVJ7ihxnzHqVOKmdpcp_3yBW22NP8_F-eof3GDtDuEYAcTOTuZYCoAIN6h1bIWiVAZR4xFagcp0VIH9-YCcpbQEQC1Eds2PMc1QFrtj23qe4Gyc_BG5Cy3szjj4888Hx9f0ar_i0If5MgbgbIvdpGClMFOaOt37cDGncmIle_z1Fk5Yt8O_G2o2JQz9bStxSpBefvKGP7L0zXaJP-3nKfnx-eLr7mj1--7K-u33MtrIClTnVqApyTU467cjoQlssUYvSamUxXySsUJISbSGEbETbgGmcUNQWZaWMPGWXb75jHH7vKE1175OlrjOBhl2qlZQCFYj_glgKlRdVtYDne3DX9NTWY_S9iXO9T3HRL_a6SdZ0LppgfTpguZS5UGrB1Bv2x3c0_3OB-rXIeimyPhRZ_3q4PRzyLxrfkPs</recordid><startdate>199209</startdate><enddate>199209</enddate><creator>Mayer, Matthias P.</creator><creator>Hahn, Frederick M.</creator><creator>Stillman, David J.</creator><creator>Poulter, C. 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Dale</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-j3807-f7b78049ef3f9fea959c161926c97c140491813e72d5223b2db0abf27ed5687a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Biological and medical sciences</topic><topic>Carbon-Carbon Double Bond Isomerases</topic><topic>chromosome</topic><topic>Chromosome Mapping</topic><topic>DNA, Fungal - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Fungal</topic><topic>Genes. Genome</topic><topic>Genetic Linkage</topic><topic>Isomerases - genetics</topic><topic>Isoprene</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Restriction Mapping</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - enzymology</topic><topic>Saccharomyces cerevisiae - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mayer, Matthias P.</creatorcontrib><creatorcontrib>Hahn, Frederick M.</creatorcontrib><creatorcontrib>Stillman, David J.</creatorcontrib><creatorcontrib>Poulter, C. 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Dale</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Disruption and mapping of IDI1, the gene for isopentenyl diphosphate isomerase in Saccharomyces cerevisiae</atitle><jtitle>Yeast (Chichester, England)</jtitle><addtitle>Yeast</addtitle><date>1992-09</date><risdate>1992</risdate><volume>8</volume><issue>9</issue><spage>743</spage><epage>748</epage><pages>743-748</pages><issn>0749-503X</issn><eissn>1097-0061</eissn><coden>YESTE3</coden><abstract>Isopontenyl diphosphate isomerase catalyses an essential activation step in the isoprene biosynthetic pathwasy. The Saccharomyces cerevisiae gene for isomerase, IDI1, was recently isolated and characterized (Anderson et al. J. Biol. Chem. 1989aa, 264, 19169–19175). Wild‐type IDI1 was disrupted with a LEU2 marker, and the resulting DNA was used to transform a yeast leucine auxotroph. Southern blots of EcoRI fragments of chromosomal DNA from the diploid strain showed the expected fragments for intact and disrupted IDI1. Dissection and analysis of tetrads demonstrated that IDI1 is an essential single‐copy gene. A CHEF gel and clone grid filter analysis, followed by chromosomal mapping indicated that the gene is located on chromosome that the gene is located on the chromosome XVI approximately 55 kb contromere proximal to PEP4.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>1441751</pmid><doi>10.1002/yea.320080907</doi><tpages>6</tpages></addata></record> |
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| ispartof | Yeast (Chichester, England), 1992-09, Vol.8 (9), p.743-748 |
| issn | 0749-503X 1097-0061 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Biological and medical sciences Carbon-Carbon Double Bond Isomerases chromosome Chromosome Mapping DNA, Fungal - genetics Fundamental and applied biological sciences. Psychology Genes, Fungal Genes. Genome Genetic Linkage Isomerases - genetics Isoprene Molecular and cellular biology Molecular genetics Restriction Mapping Saccharomyces cerevisiae Saccharomyces cerevisiae - enzymology Saccharomyces cerevisiae - genetics |
| title | Disruption and mapping of IDI1, the gene for isopentenyl diphosphate isomerase in Saccharomyces cerevisiae |
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