The 30-kilodalton gene product of tobacco mosaic virus potentiates virus movement
The proposed role of the 30-kilodalton (kD) protein of tobacco mosaic virus is to facilitate cell-to-cell spread of the virus during infection. To directly define the function of the protein, a chimeric gene containing a cloned complementary DNA of the 30-kD protein gene was introduced into tobacco...
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| Veröffentlicht in: | Science (American Association for the Advancement of Science) 1987-07, Vol.237 (4813), p.389-394 |
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| creator | Deom, C.M Oliver, M.J Beachy, R.N |
| description | The proposed role of the 30-kilodalton (kD) protein of tobacco mosaic virus is to facilitate cell-to-cell spread of the virus during infection. To directly define the function of the protein, a chimeric gene containing a cloned complementary DNA of the 30-kD protein gene was introduced into tobacco cells via a Ti plasmid-mediated transformation system of Agrobacterium tumefaciens. Transgenic plants regenerated from transformed tobacco cells expressed the 30-kD protein messenger RNA and accumulated 30-kD protein. Seedlings expressing the 30-kD protein gene complemented the Ls1 mutant of TMV, a mutant that is temperature-sensitive in cell-to-cell movement. In addition, enhanced movement of the Ls1 virus at the permissive temperature was detected in seedlings that express the 30-kD protein gene. These results conclusively demonstrate that the 30-kD protein of tobacco mosaic virus potentiates the movement of the virus from cell to cell. |
| doi_str_mv | 10.1126/science.237.4813.389 |
| format | Article |
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To directly define the function of the protein, a chimeric gene containing a cloned complementary DNA of the 30-kD protein gene was introduced into tobacco cells via a Ti plasmid-mediated transformation system of Agrobacterium tumefaciens. Transgenic plants regenerated from transformed tobacco cells expressed the 30-kD protein messenger RNA and accumulated 30-kD protein. Seedlings expressing the 30-kD protein gene complemented the Ls1 mutant of TMV, a mutant that is temperature-sensitive in cell-to-cell movement. In addition, enhanced movement of the Ls1 virus at the permissive temperature was detected in seedlings that express the 30-kD protein gene. These results conclusively demonstrate that the 30-kD protein of tobacco mosaic virus potentiates the movement of the virus from cell to cell.</description><identifier>ISSN: 0036-8075</identifier><identifier>EISSN: 1095-9203</identifier><identifier>DOI: 10.1126/science.237.4813.389</identifier><identifier>PMID: 17794341</identifier><identifier>CODEN: SCIEAS</identifier><language>eng</language><publisher>Washington, DC: The American Association for the Advancement of Science</publisher><subject>ADN ; AGROBACTERIUM ; AGROBACTERIUM TUMEFACIENS ; Biological and medical sciences ; Capsid proteins ; Cell walls ; Disease transmission ; Diseases ; DNA ; Fundamental and applied biological sciences. Psychology ; GENE ; GENES ; Infections ; Leaves ; Lesions ; Microbiology ; MOUVEMENT ; MOVEMENT ; MOVIMIENTO ; NICOTIANA TABACUM ; Phytopathology. Animal pests. 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Genetics ; Tobacco ; TOBACCO MOSAIC VIRUS ; Transgenic plants ; Viral genetics ; Virology ; VIRUS DE LA MOSAIQUE DU TABAC ; VIRUS DEL MOSAICO DEL TABACO ; Viruses</subject><ispartof>Science (American Association for the Advancement of Science), 1987-07, Vol.237 (4813), p.389-394</ispartof><rights>Copyright 1987 The American Association for the Advancement of Science</rights><rights>1988 INIST-CNRS</rights><rights>COPYRIGHT 1987 American Association for the Advancement of Science</rights><rights>COPYRIGHT 1987 American Association for the Advancement of Science</rights><rights>Copyright American Association for the Advancement of Science Jul 24, 1987</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c828t-14bb98d7c682f522a2ba88406e205e603c66d85463fee244ded2e7390902f6d83</citedby><cites>FETCH-LOGICAL-c828t-14bb98d7c682f522a2ba88406e205e603c66d85463fee244ded2e7390902f6d83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/1699558$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/1699558$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,2884,2885,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7452240$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17794341$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Deom, C.M</creatorcontrib><creatorcontrib>Oliver, M.J</creatorcontrib><creatorcontrib>Beachy, R.N</creatorcontrib><title>The 30-kilodalton gene product of tobacco mosaic virus potentiates virus movement</title><title>Science (American Association for the Advancement of Science)</title><addtitle>Science</addtitle><description>The proposed role of the 30-kilodalton (kD) protein of tobacco mosaic virus is to facilitate cell-to-cell spread of the virus during infection. To directly define the function of the protein, a chimeric gene containing a cloned complementary DNA of the 30-kD protein gene was introduced into tobacco cells via a Ti plasmid-mediated transformation system of Agrobacterium tumefaciens. Transgenic plants regenerated from transformed tobacco cells expressed the 30-kD protein messenger RNA and accumulated 30-kD protein. Seedlings expressing the 30-kD protein gene complemented the Ls1 mutant of TMV, a mutant that is temperature-sensitive in cell-to-cell movement. In addition, enhanced movement of the Ls1 virus at the permissive temperature was detected in seedlings that express the 30-kD protein gene. These results conclusively demonstrate that the 30-kD protein of tobacco mosaic virus potentiates the movement of the virus from cell to cell.</description><subject>ADN</subject><subject>AGROBACTERIUM</subject><subject>AGROBACTERIUM TUMEFACIENS</subject><subject>Biological and medical sciences</subject><subject>Capsid proteins</subject><subject>Cell walls</subject><subject>Disease transmission</subject><subject>Diseases</subject><subject>DNA</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GENE</subject><subject>GENES</subject><subject>Infections</subject><subject>Leaves</subject><subject>Lesions</subject><subject>Microbiology</subject><subject>MOUVEMENT</subject><subject>MOVEMENT</subject><subject>MOVIMIENTO</subject><subject>NICOTIANA TABACUM</subject><subject>Phytopathology. Animal pests. Plant and forest protection</subject><subject>Plant viruses and viroids</subject><subject>Plants</subject><subject>PLASMIDE</subject><subject>PLASMIDIOS</subject><subject>PLASMIDS</subject><subject>PROTEINAS</subject><subject>PROTEINE</subject><subject>PROTEINS</subject><subject>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</subject><subject>RNA</subject><subject>Seedlings</subject><subject>Systematics. Structure, properties and multiplication. Genetics</subject><subject>Tobacco</subject><subject>TOBACCO MOSAIC VIRUS</subject><subject>Transgenic plants</subject><subject>Viral genetics</subject><subject>Virology</subject><subject>VIRUS DE LA MOSAIQUE DU TABAC</subject><subject>VIRUS DEL MOSAICO DEL TABACO</subject><subject>Viruses</subject><issn>0036-8075</issn><issn>1095-9203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><recordid>eNqN011v0zAUBuAIgVgZ_IMJRQixi9HibzuXo4IyqaJC27i1XOekpCRxsZ0J_j2uGtGBKlblItI5j08i22-WnWE0wZiId8HW0FmYEConTGE6oap4lI0wKvi4IIg-zkYIUTFWSPKT7FkIa4RSr6BPsxMsZcEow6Psy803yCkaf68bV5omui5fQQf5xruytzF3VR7d0ljr8tYFU9v8rvZ9yDcuQhdrEyEMldbdQZtqz7MnlWkCvBjep9ntxw8300_j-WJ2Nb2cj60iKo4xWy4LVUorFKk4IYYsjVIMCSCIg0DUClEqzgStAAhjJZQEJC1QgUiVOvQ0O9_NTb_6o4cQdVsHC01jOnB90JLStAuckSTf_FdSQWSBKXsQEpw2DnP-IMRMpi-jLXz1D1y73ndpX9IwyinGQiR0sUMr04Cuu8pFb-z2GLxpXAdVncqXHCMmsUz67QGdnhLa2h7g53_xJCL8jCvTh6Cvrj8fKxdfj5XvZ0dKNZvflxeHpHVNAyvQ6epMF_c122nrXQgeKr3xdWv8L42R3sZDD_HQKR56Gw-d4pGWvRyOo1-2UO4XDXlI4PUATLCmqbzpbB3-OMnSTWUosbMdW4fo_H6MKArO1b5dGafNyqcJt9dKMoUQV4r-BkutJWU</recordid><startdate>19870724</startdate><enddate>19870724</enddate><creator>Deom, C.M</creator><creator>Oliver, M.J</creator><creator>Beachy, R.N</creator><general>The American Association for the Advancement of Science</general><general>American Association for the Advancement of Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8GL</scope><scope>IBG</scope><scope>IOV</scope><scope>ISN</scope><scope>7QF</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QQ</scope><scope>7QR</scope><scope>7SC</scope><scope>7SE</scope><scope>7SN</scope><scope>7SP</scope><scope>7SR</scope><scope>7SS</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7TK</scope><scope>7TM</scope><scope>7U5</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7T2</scope><scope>7U2</scope><scope>7X8</scope></search><sort><creationdate>19870724</creationdate><title>The 30-kilodalton gene product of tobacco mosaic virus potentiates virus movement</title><author>Deom, C.M ; Oliver, M.J ; Beachy, R.N</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c828t-14bb98d7c682f522a2ba88406e205e603c66d85463fee244ded2e7390902f6d83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>ADN</topic><topic>AGROBACTERIUM</topic><topic>AGROBACTERIUM TUMEFACIENS</topic><topic>Biological and medical sciences</topic><topic>Capsid proteins</topic><topic>Cell walls</topic><topic>Disease transmission</topic><topic>Diseases</topic><topic>DNA</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GENE</topic><topic>GENES</topic><topic>Infections</topic><topic>Leaves</topic><topic>Lesions</topic><topic>Microbiology</topic><topic>MOUVEMENT</topic><topic>MOVEMENT</topic><topic>MOVIMIENTO</topic><topic>NICOTIANA TABACUM</topic><topic>Phytopathology. Animal pests. Plant and forest protection</topic><topic>Plant viruses and viroids</topic><topic>Plants</topic><topic>PLASMIDE</topic><topic>PLASMIDIOS</topic><topic>PLASMIDS</topic><topic>PROTEINAS</topic><topic>PROTEINE</topic><topic>PROTEINS</topic><topic>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</topic><topic>RNA</topic><topic>Seedlings</topic><topic>Systematics. Structure, properties and multiplication. Genetics</topic><topic>Tobacco</topic><topic>TOBACCO MOSAIC VIRUS</topic><topic>Transgenic plants</topic><topic>Viral genetics</topic><topic>Virology</topic><topic>VIRUS DE LA MOSAIQUE DU TABAC</topic><topic>VIRUS DEL MOSAICO DEL TABACO</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Deom, C.M</creatorcontrib><creatorcontrib>Oliver, M.J</creatorcontrib><creatorcontrib>Beachy, R.N</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: High School</collection><collection>Biography Resource Center</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Canada</collection><collection>Aluminium Industry Abstracts</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Ecology Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Safety Science and Risk</collection><collection>MEDLINE - Academic</collection><jtitle>Science (American Association for the Advancement of Science)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Deom, C.M</au><au>Oliver, M.J</au><au>Beachy, R.N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The 30-kilodalton gene product of tobacco mosaic virus potentiates virus movement</atitle><jtitle>Science (American Association for the Advancement of Science)</jtitle><addtitle>Science</addtitle><date>1987-07-24</date><risdate>1987</risdate><volume>237</volume><issue>4813</issue><spage>389</spage><epage>394</epage><pages>389-394</pages><issn>0036-8075</issn><eissn>1095-9203</eissn><coden>SCIEAS</coden><abstract>The proposed role of the 30-kilodalton (kD) protein of tobacco mosaic virus is to facilitate cell-to-cell spread of the virus during infection. To directly define the function of the protein, a chimeric gene containing a cloned complementary DNA of the 30-kD protein gene was introduced into tobacco cells via a Ti plasmid-mediated transformation system of Agrobacterium tumefaciens. Transgenic plants regenerated from transformed tobacco cells expressed the 30-kD protein messenger RNA and accumulated 30-kD protein. Seedlings expressing the 30-kD protein gene complemented the Ls1 mutant of TMV, a mutant that is temperature-sensitive in cell-to-cell movement. In addition, enhanced movement of the Ls1 virus at the permissive temperature was detected in seedlings that express the 30-kD protein gene. These results conclusively demonstrate that the 30-kD protein of tobacco mosaic virus potentiates the movement of the virus from cell to cell.</abstract><cop>Washington, DC</cop><pub>The American Association for the Advancement of Science</pub><pmid>17794341</pmid><doi>10.1126/science.237.4813.389</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0036-8075 |
| ispartof | Science (American Association for the Advancement of Science), 1987-07, Vol.237 (4813), p.389-394 |
| issn | 0036-8075 1095-9203 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_733203542 |
| source | JSTOR; Science Online科学在线 |
| subjects | ADN AGROBACTERIUM AGROBACTERIUM TUMEFACIENS Biological and medical sciences Capsid proteins Cell walls Disease transmission Diseases DNA Fundamental and applied biological sciences. Psychology GENE GENES Infections Leaves Lesions Microbiology MOUVEMENT MOVEMENT MOVIMIENTO NICOTIANA TABACUM Phytopathology. Animal pests. Plant and forest protection Plant viruses and viroids Plants PLASMIDE PLASMIDIOS PLASMIDS PROTEINAS PROTEINE PROTEINS Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains RNA Seedlings Systematics. Structure, properties and multiplication. Genetics Tobacco TOBACCO MOSAIC VIRUS Transgenic plants Viral genetics Virology VIRUS DE LA MOSAIQUE DU TABAC VIRUS DEL MOSAICO DEL TABACO Viruses |
| title | The 30-kilodalton gene product of tobacco mosaic virus potentiates virus movement |
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