Absence of Evidence for Metabolite-Modulated Association between α-Glycerol-3-phosphate Dehydrogenase and l-Lactate Dehydrogenase

Evidence for the NADH-modulated formation of a complex between α-glycerol-3-phosphate dehydrogenase and l-lactate dehydrogenase was reported [Yong, H., Thomas, G. A., and Peticolas, W. L. (1993) Biochemistry 32, 11124−11131]. This NADH-modulated association suggested a mechanism of potentially great...

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Veröffentlicht in:	Biochemistry (Easton) 2003-05, Vol.42 (20), p.6259-6263
Hauptverfasser:	Lehoux, Eric A., Baker, Sharon M., Kovina, Marina V., Hays, Franklin A., Spivey, H. Olin
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Chromatography, Gel - methods Glycerolphosphate Dehydrogenase - chemistry Glycerolphosphate Dehydrogenase - metabolism In Vitro Techniques L-Lactate Dehydrogenase - chemistry L-Lactate Dehydrogenase - metabolism Macromolecular Substances NAD - chemistry NAD - metabolism Rabbits Sus scrofa
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creator	Lehoux, Eric A. Baker, Sharon M. Kovina, Marina V. Hays, Franklin A. Spivey, H. Olin
description	Evidence for the NADH-modulated formation of a complex between α-glycerol-3-phosphate dehydrogenase and l-lactate dehydrogenase was reported [Yong, H., Thomas, G. A., and Peticolas, W. L. (1993) Biochemistry 32, 11124−11131]. This NADH-modulated association suggested a mechanism of potentially great importance to enzyme modulation and the controversial phenomena of direct NADH channeling. In the present paper, we reproduce with additional controls the experiments described by Yong et al. ((1993) Biochemistry 32, 11124−11131). Our results conclusively demonstrate the absence of detectable association between α-glycol-3-phosphate dehydrogenase and l-lactate dehydrogenase.
doi_str_mv	10.1021/bi027244b
format	Article
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subjects	Animals Chromatography, Gel - methods Glycerolphosphate Dehydrogenase - chemistry Glycerolphosphate Dehydrogenase - metabolism In Vitro Techniques L-Lactate Dehydrogenase - chemistry L-Lactate Dehydrogenase - metabolism Macromolecular Substances NAD - chemistry NAD - metabolism Rabbits Sus scrofa
title	Absence of Evidence for Metabolite-Modulated Association between α-Glycerol-3-phosphate Dehydrogenase and l-Lactate Dehydrogenase
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