Production of poly- N-acetylglucosamine by Staphylococcus saprophyticus BMSZ711: Characterization and production optimization
This is the first report on the characterization and production optimization of poly- N-acetylglucosamine (PNAG) in Staphylococcus saprophyticus. A strain producing glucosamine exopolysaccharide was isolated and characterized by biochemical test and 16S rRNA gene sequence homology analysis and named...
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| Veröffentlicht in: | Bioresource technology 2010-09, Vol.101 (18), p.7177-7180 |
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| creator | Zamil, Sheikh Shawkat Ahmad, Shabir Choi, Mun Hwan Yoon, Sung Chul |
| description | This is the first report on the characterization and production optimization of poly-
N-acetylglucosamine (PNAG) in
Staphylococcus saprophyticus. A strain producing glucosamine exopolysaccharide was isolated and characterized by biochemical test and 16S rRNA gene sequence homology analysis and named as
S. saprophyticus BMSZ711. The molecular mass of the purified exopolymer was about 12
kDa. Digestion of the PNAG with DispersinB® proved that it has β-1,6 linkage. BMSZ711 can only produce PNAG when grown in M1 minimal medium but not in nutrient rich medium with optimum temperature of 30
°C and pH of 7. Glycerol and ammonium sulfate were found to be the best carbon and nitrogen source, respectively. Maximum PNAG production was obtained when glycerol 100
mM, ammonium sulfate 0.3%, yeast extract 1.5
g/L, sodium chloride 10
g/L and valine 2
mM were used. |
| doi_str_mv | 10.1016/j.biortech.2010.03.141 |
| format | Article |
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N-acetylglucosamine (PNAG) in
Staphylococcus saprophyticus. A strain producing glucosamine exopolysaccharide was isolated and characterized by biochemical test and 16S rRNA gene sequence homology analysis and named as
S. saprophyticus BMSZ711. The molecular mass of the purified exopolymer was about 12
kDa. Digestion of the PNAG with DispersinB® proved that it has β-1,6 linkage. BMSZ711 can only produce PNAG when grown in M1 minimal medium but not in nutrient rich medium with optimum temperature of 30
°C and pH of 7. Glycerol and ammonium sulfate were found to be the best carbon and nitrogen source, respectively. Maximum PNAG production was obtained when glycerol 100
mM, ammonium sulfate 0.3%, yeast extract 1.5
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mM were used.</description><identifier>ISSN: 0960-8524</identifier><identifier>EISSN: 1873-2976</identifier><identifier>DOI: 10.1016/j.biortech.2010.03.141</identifier><identifier>PMID: 20456948</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>Acetylglucosamine - metabolism ; Ammonium sulfates ; Biochemistry ; Biological and medical sciences ; Carbon ; Cell Culture Techniques - methods ; Exopolysaccharide ; Fundamental and applied biological sciences. Psychology ; Genes ; Glucosamine ; Glycerols ; Optimization ; PNAG production optimization ; Poly- N-acetylglucosamine (PNAG) ; Species Specificity ; Staphylococcus ; Staphylococcus - classification ; Staphylococcus - genetics ; Staphylococcus - metabolism ; Staphylococcus saprophyticus ; Strain</subject><ispartof>Bioresource technology, 2010-09, Vol.101 (18), p.7177-7180</ispartof><rights>2010 Elsevier Ltd</rights><rights>2015 INIST-CNRS</rights><rights>Copyright 2010 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c500t-ae1cf6a18cfa0ca0abef75e987fee03d328b9a7963ea06960946d6bf9d516813</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0960852410006607$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22913722$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20456948$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zamil, Sheikh Shawkat</creatorcontrib><creatorcontrib>Ahmad, Shabir</creatorcontrib><creatorcontrib>Choi, Mun Hwan</creatorcontrib><creatorcontrib>Yoon, Sung Chul</creatorcontrib><title>Production of poly- N-acetylglucosamine by Staphylococcus saprophyticus BMSZ711: Characterization and production optimization</title><title>Bioresource technology</title><addtitle>Bioresour Technol</addtitle><description>This is the first report on the characterization and production optimization of poly-
N-acetylglucosamine (PNAG) in
Staphylococcus saprophyticus. A strain producing glucosamine exopolysaccharide was isolated and characterized by biochemical test and 16S rRNA gene sequence homology analysis and named as
S. saprophyticus BMSZ711. The molecular mass of the purified exopolymer was about 12
kDa. Digestion of the PNAG with DispersinB® proved that it has β-1,6 linkage. BMSZ711 can only produce PNAG when grown in M1 minimal medium but not in nutrient rich medium with optimum temperature of 30
°C and pH of 7. Glycerol and ammonium sulfate were found to be the best carbon and nitrogen source, respectively. Maximum PNAG production was obtained when glycerol 100
mM, ammonium sulfate 0.3%, yeast extract 1.5
g/L, sodium chloride 10
g/L and valine 2
mM were used.</description><subject>Acetylglucosamine - metabolism</subject><subject>Ammonium sulfates</subject><subject>Biochemistry</subject><subject>Biological and medical sciences</subject><subject>Carbon</subject><subject>Cell Culture Techniques - methods</subject><subject>Exopolysaccharide</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes</subject><subject>Glucosamine</subject><subject>Glycerols</subject><subject>Optimization</subject><subject>PNAG production optimization</subject><subject>Poly- N-acetylglucosamine (PNAG)</subject><subject>Species Specificity</subject><subject>Staphylococcus</subject><subject>Staphylococcus - classification</subject><subject>Staphylococcus - genetics</subject><subject>Staphylococcus - metabolism</subject><subject>Staphylococcus saprophyticus</subject><subject>Strain</subject><issn>0960-8524</issn><issn>1873-2976</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2P1DAMhiMEYoeFv7DqBcGlQz7aJOUEjPiSlg9p98QlclOXyajTdJMUqUj8dzLMLHCCUxT7sf3aLyEXjK4ZZfLZbt06HxLa7ZrTHKRizSp2h6yYVqLkjZJ3yYo2kpa65tUZeRDjjlIqmOL3yRmnVS2bSq_Ij8_Bd7NNzo-F74vJD0tZfCzBYlqGr8NsfYS9G7Fol-IqwbRdBm-9tXMsIkzB50Byh9-rD1dfFGPPi80WAtiEwX2HX21h7IrprylTcvtT7iG518MQ8dHpPSfXb15fb96Vl5_evt-8vCxtTWkqAZntJTBte6AWKLTYqxobrXpEKjrBdduAaqRAoDIv3VSyk23fdDWTmolz8uTYNsu4mTEms3fR4jDAiH6ORgnBdF1pncmn_ySZUorVglc8o_KI2uBjDNibKbg9hMUwag4emZ259cgcPDJUmOxRLrw4zZjbPXa_y25NycDjEwDRwtAHGK2LfzjeMKH4QcGLI4f5dN8cBhOtw9Fi5wLaZDrv_qflJ_-JteM</recordid><startdate>20100901</startdate><enddate>20100901</enddate><creator>Zamil, Sheikh Shawkat</creator><creator>Ahmad, Shabir</creator><creator>Choi, Mun Hwan</creator><creator>Yoon, Sung Chul</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SU</scope><scope>7TB</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>KR7</scope><scope>7X8</scope></search><sort><creationdate>20100901</creationdate><title>Production of poly- N-acetylglucosamine by Staphylococcus saprophyticus BMSZ711: Characterization and production optimization</title><author>Zamil, Sheikh Shawkat ; Ahmad, Shabir ; Choi, Mun Hwan ; Yoon, Sung Chul</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c500t-ae1cf6a18cfa0ca0abef75e987fee03d328b9a7963ea06960946d6bf9d516813</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Acetylglucosamine - metabolism</topic><topic>Ammonium sulfates</topic><topic>Biochemistry</topic><topic>Biological and medical sciences</topic><topic>Carbon</topic><topic>Cell Culture Techniques - methods</topic><topic>Exopolysaccharide</topic><topic>Fundamental and applied biological sciences. 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N-acetylglucosamine (PNAG) in
Staphylococcus saprophyticus. A strain producing glucosamine exopolysaccharide was isolated and characterized by biochemical test and 16S rRNA gene sequence homology analysis and named as
S. saprophyticus BMSZ711. The molecular mass of the purified exopolymer was about 12
kDa. Digestion of the PNAG with DispersinB® proved that it has β-1,6 linkage. BMSZ711 can only produce PNAG when grown in M1 minimal medium but not in nutrient rich medium with optimum temperature of 30
°C and pH of 7. Glycerol and ammonium sulfate were found to be the best carbon and nitrogen source, respectively. Maximum PNAG production was obtained when glycerol 100
mM, ammonium sulfate 0.3%, yeast extract 1.5
g/L, sodium chloride 10
g/L and valine 2
mM were used.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>20456948</pmid><doi>10.1016/j.biortech.2010.03.141</doi><tpages>4</tpages></addata></record> |
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| issn | 0960-8524 1873-2976 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Acetylglucosamine - metabolism Ammonium sulfates Biochemistry Biological and medical sciences Carbon Cell Culture Techniques - methods Exopolysaccharide Fundamental and applied biological sciences. Psychology Genes Glucosamine Glycerols Optimization PNAG production optimization Poly- N-acetylglucosamine (PNAG) Species Specificity Staphylococcus Staphylococcus - classification Staphylococcus - genetics Staphylococcus - metabolism Staphylococcus saprophyticus Strain |
| title | Production of poly- N-acetylglucosamine by Staphylococcus saprophyticus BMSZ711: Characterization and production optimization |
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