Autocalibrated Scanning-Angle Prism-Type Total Internal Reflection Fluorescence Microscopy for Nanometer-Precision Axial Position Determination

An automatic calibration and scanning-angle prism-type total internal reflection fluorescence microscope (TIRFM) was constructed and tested for the highest vertical resolution. The angle of the incident laser beam can be changed automatically and reliably from subcritical angles to nearly 90° with i...

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Veröffentlicht in:	Analytical chemistry (Washington) 2010-03, Vol.82 (6), p.2441-2447
Hauptverfasser:	Sun, Wei, Marchuk, Kyle, Wang, Gufeng, Fang, Ning
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical chemistry Calibration Chemistry Equipment Design Exact sciences and technology Lasers Microscopy Microscopy, Fluorescence - instrumentation Microscopy, Fluorescence - methods Microtubules - ultrastructure Monte Carlo simulation Spectrometric and optical methods Surface Plasmon Resonance
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