A novel type of family 19 chitinase from Aeromonas sp. No.10S-24. Cloning, sequence, expression, and the enzymatic properties

A family 19 chitinase gene from Aeromonas sp. No.10S-24 was cloned, sequenced, and expressed in Escherichia coli. From the deduced amino acid sequence, the enzyme was found to possess two repeated N-terminal chitin-binding domains, which are separated by two proline-threonine rich linkers. The calcu...

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Veröffentlicht in:	European journal of biochemistry 2003-06, Vol.270 (11), p.2513-2520
Hauptverfasser:	Ueda, Mitsuhiro, Kojima, Miki, Yoshikawa, Tomoye, Mitsuda, Norio, Araki, Keiichi, Kawaguchi, Takashi, Miyatake, Kazutaka, Arai, Motoo, Fukamizo, Tamo
Format:	Artikel
Sprache:	eng
Schlagworte:	Aeromonas - genetics Aeromonas - metabolism Amino Acid Sequence Base Sequence Chitinases - chemistry Chitinases - classification Chitinases - genetics Chromatography, High Pressure Liquid Cloning, Molecular DNA - metabolism Electrophoresis, Polyacrylamide Gel Escherichia coli - metabolism Gene Library Genetic Vectors Glycosides - metabolism Hydrolysis Molecular Sequence Data Oligosaccharides - chemistry Plasmids - metabolism Protein Binding Protein Structure, Tertiary Recombinant Proteins - chemistry Sequence Analysis, DNA Sequence Homology, Amino Acid Time Factors
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