HBD-1 and hBD-2 are expressed in cervico-vaginal lavage in female genital tract due to microbial infections
The aim of this study was to evaluate and compare concentration of selected human beta-defensins (hBD-1, hBD-2) in cervico-vaginal lavage (CVL), obtained from women with candidiasis, chlamydiasis and other bacterial infections. beta-defensins were detected quantitatively by RT-PCR (7000 Taqman, Appl...
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| Veröffentlicht in: | Ginekologia polska 2010-04, Vol.81 (4), p.268-271 |
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| creator | Wiechuła, Barbara Cholewa, Krzysztof Ekiel, Alicja Romanik, Małgorzata Dolezych, Hanna Martirosian, Gayane |
| description | The aim of this study was to evaluate and compare concentration of selected human beta-defensins (hBD-1, hBD-2) in cervico-vaginal lavage (CVL), obtained from women with candidiasis, chlamydiasis and other bacterial infections.
beta-defensins were detected quantitatively by RT-PCR (7000 Taqman, Applied Biosystems) in cervico-vaginal lavage collected from 120 (79 women in the study group and 41 controls) non-pregnant women, aged 18-40 (mean age 28.5 +/- 6.29). The study group patients were divided into three subgroups on the basis of clinical and microbiological diagnosis: women with candidiasis (n=13); with chlamydiasis (n=13), and with other bacterial infections (n=12).
The highest count of hBD-1 RNA copies was found in women with bacterial infections and candidiasis (335.84 and 320.10 respectively), and hBD-2--with chlamydiasis. The difference between RNA copies of hBD-1/microg in candidiasis, chlamydiasis and bacterial pathogens was statistically significant; for hBD-2 only in case of chlamydiasis.
Chlamydia trachomatis infection activates the production of hBD-2. Candida albicans, Chlamydia trachomatis, and bacterial pathogens induced variable increases of hBD-1 concentration. |
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beta-defensins were detected quantitatively by RT-PCR (7000 Taqman, Applied Biosystems) in cervico-vaginal lavage collected from 120 (79 women in the study group and 41 controls) non-pregnant women, aged 18-40 (mean age 28.5 +/- 6.29). The study group patients were divided into three subgroups on the basis of clinical and microbiological diagnosis: women with candidiasis (n=13); with chlamydiasis (n=13), and with other bacterial infections (n=12).
The highest count of hBD-1 RNA copies was found in women with bacterial infections and candidiasis (335.84 and 320.10 respectively), and hBD-2--with chlamydiasis. The difference between RNA copies of hBD-1/microg in candidiasis, chlamydiasis and bacterial pathogens was statistically significant; for hBD-2 only in case of chlamydiasis.
Chlamydia trachomatis infection activates the production of hBD-2. Candida albicans, Chlamydia trachomatis, and bacterial pathogens induced variable increases of hBD-1 concentration.</description><identifier>ISSN: 0017-0011</identifier><identifier>EISSN: 2543-6767</identifier><identifier>PMID: 20476598</identifier><language>pol</language><publisher>Poland: Wydawnictwo Via Medica</publisher><subject>Adult ; Bacterial infections ; beta-Defensins - metabolism ; Biomarkers - metabolism ; Candidiasis, Vulvovaginal - diagnosis ; Candidiasis, Vulvovaginal - metabolism ; Cervix Mucus - microbiology ; Chlamydia ; Chlamydia Infections - diagnosis ; Chlamydia Infections - metabolism ; Colony Count, Microbial ; Female ; Genital Diseases, Female - diagnosis ; Genital Diseases, Female - metabolism ; Genital Diseases, Female - microbiology ; Humans ; Infections ; Pathogens ; Reverse Transcriptase Polymerase Chain Reaction ; Sexually Transmitted Diseases, Bacterial - diagnosis ; Sexually Transmitted Diseases, Bacterial - metabolism ; Vagina ; Vagina - metabolism ; Vaginal Smears - methods ; Young Adult</subject><ispartof>Ginekologia polska, 2010-04, Vol.81 (4), p.268-271</ispartof><rights>2010. This work is published under https://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20476598$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wiechuła, Barbara</creatorcontrib><creatorcontrib>Cholewa, Krzysztof</creatorcontrib><creatorcontrib>Ekiel, Alicja</creatorcontrib><creatorcontrib>Romanik, Małgorzata</creatorcontrib><creatorcontrib>Dolezych, Hanna</creatorcontrib><creatorcontrib>Martirosian, Gayane</creatorcontrib><title>HBD-1 and hBD-2 are expressed in cervico-vaginal lavage in female genital tract due to microbial infections</title><title>Ginekologia polska</title><addtitle>Ginekol Pol</addtitle><description>The aim of this study was to evaluate and compare concentration of selected human beta-defensins (hBD-1, hBD-2) in cervico-vaginal lavage (CVL), obtained from women with candidiasis, chlamydiasis and other bacterial infections.
beta-defensins were detected quantitatively by RT-PCR (7000 Taqman, Applied Biosystems) in cervico-vaginal lavage collected from 120 (79 women in the study group and 41 controls) non-pregnant women, aged 18-40 (mean age 28.5 +/- 6.29). The study group patients were divided into three subgroups on the basis of clinical and microbiological diagnosis: women with candidiasis (n=13); with chlamydiasis (n=13), and with other bacterial infections (n=12).
The highest count of hBD-1 RNA copies was found in women with bacterial infections and candidiasis (335.84 and 320.10 respectively), and hBD-2--with chlamydiasis. The difference between RNA copies of hBD-1/microg in candidiasis, chlamydiasis and bacterial pathogens was statistically significant; for hBD-2 only in case of chlamydiasis.
Chlamydia trachomatis infection activates the production of hBD-2. Candida albicans, Chlamydia trachomatis, and bacterial pathogens induced variable increases of hBD-1 concentration.</description><subject>Adult</subject><subject>Bacterial infections</subject><subject>beta-Defensins - metabolism</subject><subject>Biomarkers - metabolism</subject><subject>Candidiasis, Vulvovaginal - diagnosis</subject><subject>Candidiasis, Vulvovaginal - metabolism</subject><subject>Cervix Mucus - microbiology</subject><subject>Chlamydia</subject><subject>Chlamydia Infections - diagnosis</subject><subject>Chlamydia Infections - metabolism</subject><subject>Colony Count, Microbial</subject><subject>Female</subject><subject>Genital Diseases, Female - diagnosis</subject><subject>Genital Diseases, Female - metabolism</subject><subject>Genital Diseases, Female - microbiology</subject><subject>Humans</subject><subject>Infections</subject><subject>Pathogens</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Sexually Transmitted Diseases, Bacterial - diagnosis</subject><subject>Sexually Transmitted Diseases, Bacterial - metabolism</subject><subject>Vagina</subject><subject>Vagina - metabolism</subject><subject>Vaginal Smears - methods</subject><subject>Young Adult</subject><issn>0017-0011</issn><issn>2543-6767</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNpdkE1LAzEQhoMottT-BQl48LSQTbLJ5qj1o0LBi56X2c2kpu5HTXaL_nsj1otzmHmZ9-GFmRMy54UUmdJKn5I5Y7nOUstnZBnjjqVSXHNjzsmMM6lVYco5eV_f3mU5hd7St6Q4hYAUP_cBY0RLfU8bDAffDNkBtr6HlraQFP44DjtokW6x92MyxgDNSO2EdBxo55sw1D6tfe-wGf3Qxwty5qCNuDzOBXl9uH9ZrbPN8-PT6maT7XNVjhmUmkHBBBqwTIq8kEoxw5yQ0kHNlctLLAQoY3UtmAQhmdVGMKcLy03txIJc_-buw_AxYRyrzscG2xZ6HKZYaZFCDZcikVf_yN0whXRlrLhUkrPS6CJRl0dqqju01T74DsJX9fdF8Q3qH21n</recordid><startdate>20100401</startdate><enddate>20100401</enddate><creator>Wiechuła, Barbara</creator><creator>Cholewa, Krzysztof</creator><creator>Ekiel, Alicja</creator><creator>Romanik, Małgorzata</creator><creator>Dolezych, Hanna</creator><creator>Martirosian, Gayane</creator><general>Wydawnictwo Via Medica</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20100401</creationdate><title>HBD-1 and hBD-2 are expressed in cervico-vaginal lavage in female genital tract due to microbial infections</title><author>Wiechuła, Barbara ; Cholewa, Krzysztof ; Ekiel, Alicja ; Romanik, Małgorzata ; Dolezych, Hanna ; Martirosian, Gayane</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p168t-a870a503e9ad04315466090f344fab26f18e53a69d7b304a340d7930f75d29bf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>pol</language><creationdate>2010</creationdate><topic>Adult</topic><topic>Bacterial infections</topic><topic>beta-Defensins - metabolism</topic><topic>Biomarkers - metabolism</topic><topic>Candidiasis, Vulvovaginal - diagnosis</topic><topic>Candidiasis, Vulvovaginal - metabolism</topic><topic>Cervix Mucus - microbiology</topic><topic>Chlamydia</topic><topic>Chlamydia Infections - diagnosis</topic><topic>Chlamydia Infections - metabolism</topic><topic>Colony Count, Microbial</topic><topic>Female</topic><topic>Genital Diseases, Female - diagnosis</topic><topic>Genital Diseases, Female - metabolism</topic><topic>Genital Diseases, Female - microbiology</topic><topic>Humans</topic><topic>Infections</topic><topic>Pathogens</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Sexually Transmitted Diseases, Bacterial - diagnosis</topic><topic>Sexually Transmitted Diseases, Bacterial - metabolism</topic><topic>Vagina</topic><topic>Vagina - metabolism</topic><topic>Vaginal Smears - methods</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wiechuła, Barbara</creatorcontrib><creatorcontrib>Cholewa, Krzysztof</creatorcontrib><creatorcontrib>Ekiel, Alicja</creatorcontrib><creatorcontrib>Romanik, Małgorzata</creatorcontrib><creatorcontrib>Dolezych, Hanna</creatorcontrib><creatorcontrib>Martirosian, Gayane</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Ginekologia polska</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wiechuła, Barbara</au><au>Cholewa, Krzysztof</au><au>Ekiel, Alicja</au><au>Romanik, Małgorzata</au><au>Dolezych, Hanna</au><au>Martirosian, Gayane</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>HBD-1 and hBD-2 are expressed in cervico-vaginal lavage in female genital tract due to microbial infections</atitle><jtitle>Ginekologia polska</jtitle><addtitle>Ginekol Pol</addtitle><date>2010-04-01</date><risdate>2010</risdate><volume>81</volume><issue>4</issue><spage>268</spage><epage>271</epage><pages>268-271</pages><issn>0017-0011</issn><eissn>2543-6767</eissn><abstract>The aim of this study was to evaluate and compare concentration of selected human beta-defensins (hBD-1, hBD-2) in cervico-vaginal lavage (CVL), obtained from women with candidiasis, chlamydiasis and other bacterial infections.
beta-defensins were detected quantitatively by RT-PCR (7000 Taqman, Applied Biosystems) in cervico-vaginal lavage collected from 120 (79 women in the study group and 41 controls) non-pregnant women, aged 18-40 (mean age 28.5 +/- 6.29). The study group patients were divided into three subgroups on the basis of clinical and microbiological diagnosis: women with candidiasis (n=13); with chlamydiasis (n=13), and with other bacterial infections (n=12).
The highest count of hBD-1 RNA copies was found in women with bacterial infections and candidiasis (335.84 and 320.10 respectively), and hBD-2--with chlamydiasis. The difference between RNA copies of hBD-1/microg in candidiasis, chlamydiasis and bacterial pathogens was statistically significant; for hBD-2 only in case of chlamydiasis.
Chlamydia trachomatis infection activates the production of hBD-2. Candida albicans, Chlamydia trachomatis, and bacterial pathogens induced variable increases of hBD-1 concentration.</abstract><cop>Poland</cop><pub>Wydawnictwo Via Medica</pub><pmid>20476598</pmid><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adult Bacterial infections beta-Defensins - metabolism Biomarkers - metabolism Candidiasis, Vulvovaginal - diagnosis Candidiasis, Vulvovaginal - metabolism Cervix Mucus - microbiology Chlamydia Chlamydia Infections - diagnosis Chlamydia Infections - metabolism Colony Count, Microbial Female Genital Diseases, Female - diagnosis Genital Diseases, Female - metabolism Genital Diseases, Female - microbiology Humans Infections Pathogens Reverse Transcriptase Polymerase Chain Reaction Sexually Transmitted Diseases, Bacterial - diagnosis Sexually Transmitted Diseases, Bacterial - metabolism Vagina Vagina - metabolism Vaginal Smears - methods Young Adult |
| title | HBD-1 and hBD-2 are expressed in cervico-vaginal lavage in female genital tract due to microbial infections |
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