The effect of TGFα on intestinal solute transport
The effect of transforming growth α (TGFα) and epidermal growth factor (EGF) on 3-O- methylglucose transport was examined in vitro under short-circuited conditions in stripped rabbit jejunum. Mucosal EGF, 60 ng/ml, stimulated a significant increase in net 3-O- methylglucose transport ( J net 0.67 ±...
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| Veröffentlicht in: | Regulatory peptides 1992-06, Vol.39 (2), p.169-176 |
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| creator | Hardin, J.A. Gall, D.Grant |
| description | The effect of transforming growth α (TGFα) and epidermal growth factor (EGF) on
3-O-
methylglucose
transport was examined in vitro under short-circuited conditions in stripped rabbit jejunum. Mucosal EGF, 60 ng/ml, stimulated a significant increase in net
3-O-
methylglucose
transport (
J
net
0.67 ± 0.15
vs.
0.90 ± 0.15 μ
Eq/cm
2/
h
;
P < 0.03;
n = 6) due to an increased mucosal to serosal flux (
J
ms
1.2 ± 0.2
vs.
1.5 ± 0.2 μ
Eq/cm
2/
h
;
P < 0.03). In contrast, TGFα, when applied to both mucosal and serosal surfaces at concentrations of either 60 (
n = 6) or 150 (
n = 9) ng/ml had no effect on either mucosal to serosal (
J
ms
) or net transport (
J
net
) of
3-O-
methylglucose
. TGFα did induce a significant increase in the serosal to mucosal flux (
J
sm
60
ng/ml 0.44 ± 0.02
vs.
0.51 ± 0.03, 150 ng/ml
0.55 ± 0.03 vs.
0.64 ± 0.05 μ
Eq/cm
2/
h
;
P < 0.05). When brush border surface area was examined after exposure to either 60 ng/ml TGFα or saline vehicle for 2 h in in vivo isolated jejunal loops no significant difference was found (control
53 ± 1.9;
n = 35 vs. TGFα
52 ± 1.9 μ
m
2
;
n = 29). Bioactivity of transforming growth factor α was assessed by an gastric acid secretion bioassay and found to be intact. These data provide further evidence for separate and distinct functional roles for these peptides in some biological systems. |
| doi_str_mv | 10.1016/0167-0115(92)90538-6 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_73315837</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0167011592905386</els_id><sourcerecordid>73315837</sourcerecordid><originalsourceid>FETCH-LOGICAL-c386t-ad48414716b862a299782731e0d7bc1320c0394269a70496d2459485066742f93</originalsourceid><addsrcrecordid>eNp9kMtKAzEUhoMotV7eQGEWIroYzW1y2QhSbBUKbuo6pJkMRqaTmmQEH8sX8ZlMnVJ3Lg5ncb7_cM4HwBmCNwgidpuLlxCh6kriawkrIkq2B8ZIcFIiJtg-GO-QQ3AU4xuEqOKcjMAIUSIkh2OAF6-2sE1jTSp8Uyxm0--vwneF65KNyXW6LaJv-2SLFHQX1z6kE3DQ6Dba020_Bi_Th8XksZw_z54m9_PSEMFSqWsqKKIcsaVgWGMpucCcIAtrvjSIYGggkRQzqTmkktWYVpKKCjLGKW4kOQaXw9518O99vkatXDS2bXVnfR8VJwRVgvAM0gE0wccYbKPWwa10-FQIqo0qtfGgNh6UxOpXlWI5dr7d3y9Xtv4LDW7y_GI719Hotsn_Gxd3WEVIBmnG7gbMZhcfzgYVjbOdsbUL2aqqvfv_jh8ma4GX</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>73315837</pqid></control><display><type>article</type><title>The effect of TGFα on intestinal solute transport</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><creator>Hardin, J.A. ; Gall, D.Grant</creator><creatorcontrib>Hardin, J.A. ; Gall, D.Grant</creatorcontrib><description>The effect of transforming growth α (TGFα) and epidermal growth factor (EGF) on
3-O-
methylglucose
transport was examined in vitro under short-circuited conditions in stripped rabbit jejunum. Mucosal EGF, 60 ng/ml, stimulated a significant increase in net
3-O-
methylglucose
transport (
J
net
0.67 ± 0.15
vs.
0.90 ± 0.15 μ
Eq/cm
2/
h
;
P < 0.03;
n = 6) due to an increased mucosal to serosal flux (
J
ms
1.2 ± 0.2
vs.
1.5 ± 0.2 μ
Eq/cm
2/
h
;
P < 0.03). In contrast, TGFα, when applied to both mucosal and serosal surfaces at concentrations of either 60 (
n = 6) or 150 (
n = 9) ng/ml had no effect on either mucosal to serosal (
J
ms
) or net transport (
J
net
) of
3-O-
methylglucose
. TGFα did induce a significant increase in the serosal to mucosal flux (
J
sm
60
ng/ml 0.44 ± 0.02
vs.
0.51 ± 0.03, 150 ng/ml
0.55 ± 0.03 vs.
0.64 ± 0.05 μ
Eq/cm
2/
h
;
P < 0.05). When brush border surface area was examined after exposure to either 60 ng/ml TGFα or saline vehicle for 2 h in in vivo isolated jejunal loops no significant difference was found (control
53 ± 1.9;
n = 35 vs. TGFα
52 ± 1.9 μ
m
2
;
n = 29). Bioactivity of transforming growth factor α was assessed by an gastric acid secretion bioassay and found to be intact. These data provide further evidence for separate and distinct functional roles for these peptides in some biological systems.</description><identifier>ISSN: 0167-0115</identifier><identifier>EISSN: 1873-1686</identifier><identifier>DOI: 10.1016/0167-0115(92)90538-6</identifier><identifier>PMID: 1438970</identifier><identifier>CODEN: REPPDY</identifier><language>eng</language><publisher>Shannon: Elsevier B.V</publisher><subject>3-O-Methylglucose ; Animals ; Biological and medical sciences ; Biological Assay ; Biological Transport, Active - drug effects ; Epidermal growth factor ; Epidermal Growth Factor - pharmacology ; Fundamental and applied biological sciences. Psychology ; In Vitro Techniques ; Intestinal Mucosa - drug effects ; Intestinal Mucosa - metabolism ; Intestinal Mucosa - ultrastructure ; Intestinal solute transport ; Intestine. Mesentery ; Jejunum - drug effects ; Jejunum - metabolism ; Jejunum - ultrastructure ; Methylglucosides - metabolism ; Microscopy, Electron ; Microvilli - drug effects ; Microvilli - ultrastructure ; Perfusion ; Rabbits ; TGFα ; Transforming Growth Factor alpha - analysis ; Transforming Growth Factor alpha - pharmacology ; Vertebrates: digestive system</subject><ispartof>Regulatory peptides, 1992-06, Vol.39 (2), p.169-176</ispartof><rights>1992</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-ad48414716b862a299782731e0d7bc1320c0394269a70496d2459485066742f93</citedby><cites>FETCH-LOGICAL-c386t-ad48414716b862a299782731e0d7bc1320c0394269a70496d2459485066742f93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0167-0115(92)90538-6$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>309,310,314,780,784,789,790,3550,23930,23931,25140,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5334384$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1438970$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hardin, J.A.</creatorcontrib><creatorcontrib>Gall, D.Grant</creatorcontrib><title>The effect of TGFα on intestinal solute transport</title><title>Regulatory peptides</title><addtitle>Regul Pept</addtitle><description>The effect of transforming growth α (TGFα) and epidermal growth factor (EGF) on
3-O-
methylglucose
transport was examined in vitro under short-circuited conditions in stripped rabbit jejunum. Mucosal EGF, 60 ng/ml, stimulated a significant increase in net
3-O-
methylglucose
transport (
J
net
0.67 ± 0.15
vs.
0.90 ± 0.15 μ
Eq/cm
2/
h
;
P < 0.03;
n = 6) due to an increased mucosal to serosal flux (
J
ms
1.2 ± 0.2
vs.
1.5 ± 0.2 μ
Eq/cm
2/
h
;
P < 0.03). In contrast, TGFα, when applied to both mucosal and serosal surfaces at concentrations of either 60 (
n = 6) or 150 (
n = 9) ng/ml had no effect on either mucosal to serosal (
J
ms
) or net transport (
J
net
) of
3-O-
methylglucose
. TGFα did induce a significant increase in the serosal to mucosal flux (
J
sm
60
ng/ml 0.44 ± 0.02
vs.
0.51 ± 0.03, 150 ng/ml
0.55 ± 0.03 vs.
0.64 ± 0.05 μ
Eq/cm
2/
h
;
P < 0.05). When brush border surface area was examined after exposure to either 60 ng/ml TGFα or saline vehicle for 2 h in in vivo isolated jejunal loops no significant difference was found (control
53 ± 1.9;
n = 35 vs. TGFα
52 ± 1.9 μ
m
2
;
n = 29). Bioactivity of transforming growth factor α was assessed by an gastric acid secretion bioassay and found to be intact. These data provide further evidence for separate and distinct functional roles for these peptides in some biological systems.</description><subject>3-O-Methylglucose</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biological Assay</subject><subject>Biological Transport, Active - drug effects</subject><subject>Epidermal growth factor</subject><subject>Epidermal Growth Factor - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>In Vitro Techniques</subject><subject>Intestinal Mucosa - drug effects</subject><subject>Intestinal Mucosa - metabolism</subject><subject>Intestinal Mucosa - ultrastructure</subject><subject>Intestinal solute transport</subject><subject>Intestine. Mesentery</subject><subject>Jejunum - drug effects</subject><subject>Jejunum - metabolism</subject><subject>Jejunum - ultrastructure</subject><subject>Methylglucosides - metabolism</subject><subject>Microscopy, Electron</subject><subject>Microvilli - drug effects</subject><subject>Microvilli - ultrastructure</subject><subject>Perfusion</subject><subject>Rabbits</subject><subject>TGFα</subject><subject>Transforming Growth Factor alpha - analysis</subject><subject>Transforming Growth Factor alpha - pharmacology</subject><subject>Vertebrates: digestive system</subject><issn>0167-0115</issn><issn>1873-1686</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtKAzEUhoMotV7eQGEWIroYzW1y2QhSbBUKbuo6pJkMRqaTmmQEH8sX8ZlMnVJ3Lg5ncb7_cM4HwBmCNwgidpuLlxCh6kriawkrIkq2B8ZIcFIiJtg-GO-QQ3AU4xuEqOKcjMAIUSIkh2OAF6-2sE1jTSp8Uyxm0--vwneF65KNyXW6LaJv-2SLFHQX1z6kE3DQ6Dba020_Bi_Th8XksZw_z54m9_PSEMFSqWsqKKIcsaVgWGMpucCcIAtrvjSIYGggkRQzqTmkktWYVpKKCjLGKW4kOQaXw9518O99vkatXDS2bXVnfR8VJwRVgvAM0gE0wccYbKPWwa10-FQIqo0qtfGgNh6UxOpXlWI5dr7d3y9Xtv4LDW7y_GI719Hotsn_Gxd3WEVIBmnG7gbMZhcfzgYVjbOdsbUL2aqqvfv_jh8ma4GX</recordid><startdate>19920611</startdate><enddate>19920611</enddate><creator>Hardin, J.A.</creator><creator>Gall, D.Grant</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19920611</creationdate><title>The effect of TGFα on intestinal solute transport</title><author>Hardin, J.A. ; Gall, D.Grant</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-ad48414716b862a299782731e0d7bc1320c0394269a70496d2459485066742f93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>3-O-Methylglucose</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biological Assay</topic><topic>Biological Transport, Active - drug effects</topic><topic>Epidermal growth factor</topic><topic>Epidermal Growth Factor - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>In Vitro Techniques</topic><topic>Intestinal Mucosa - drug effects</topic><topic>Intestinal Mucosa - metabolism</topic><topic>Intestinal Mucosa - ultrastructure</topic><topic>Intestinal solute transport</topic><topic>Intestine. Mesentery</topic><topic>Jejunum - drug effects</topic><topic>Jejunum - metabolism</topic><topic>Jejunum - ultrastructure</topic><topic>Methylglucosides - metabolism</topic><topic>Microscopy, Electron</topic><topic>Microvilli - drug effects</topic><topic>Microvilli - ultrastructure</topic><topic>Perfusion</topic><topic>Rabbits</topic><topic>TGFα</topic><topic>Transforming Growth Factor alpha - analysis</topic><topic>Transforming Growth Factor alpha - pharmacology</topic><topic>Vertebrates: digestive system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hardin, J.A.</creatorcontrib><creatorcontrib>Gall, D.Grant</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Regulatory peptides</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hardin, J.A.</au><au>Gall, D.Grant</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The effect of TGFα on intestinal solute transport</atitle><jtitle>Regulatory peptides</jtitle><addtitle>Regul Pept</addtitle><date>1992-06-11</date><risdate>1992</risdate><volume>39</volume><issue>2</issue><spage>169</spage><epage>176</epage><pages>169-176</pages><issn>0167-0115</issn><eissn>1873-1686</eissn><coden>REPPDY</coden><abstract>The effect of transforming growth α (TGFα) and epidermal growth factor (EGF) on
3-O-
methylglucose
transport was examined in vitro under short-circuited conditions in stripped rabbit jejunum. Mucosal EGF, 60 ng/ml, stimulated a significant increase in net
3-O-
methylglucose
transport (
J
net
0.67 ± 0.15
vs.
0.90 ± 0.15 μ
Eq/cm
2/
h
;
P < 0.03;
n = 6) due to an increased mucosal to serosal flux (
J
ms
1.2 ± 0.2
vs.
1.5 ± 0.2 μ
Eq/cm
2/
h
;
P < 0.03). In contrast, TGFα, when applied to both mucosal and serosal surfaces at concentrations of either 60 (
n = 6) or 150 (
n = 9) ng/ml had no effect on either mucosal to serosal (
J
ms
) or net transport (
J
net
) of
3-O-
methylglucose
. TGFα did induce a significant increase in the serosal to mucosal flux (
J
sm
60
ng/ml 0.44 ± 0.02
vs.
0.51 ± 0.03, 150 ng/ml
0.55 ± 0.03 vs.
0.64 ± 0.05 μ
Eq/cm
2/
h
;
P < 0.05). When brush border surface area was examined after exposure to either 60 ng/ml TGFα or saline vehicle for 2 h in in vivo isolated jejunal loops no significant difference was found (control
53 ± 1.9;
n = 35 vs. TGFα
52 ± 1.9 μ
m
2
;
n = 29). Bioactivity of transforming growth factor α was assessed by an gastric acid secretion bioassay and found to be intact. These data provide further evidence for separate and distinct functional roles for these peptides in some biological systems.</abstract><cop>Shannon</cop><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>1438970</pmid><doi>10.1016/0167-0115(92)90538-6</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0167-0115 |
| ispartof | Regulatory peptides, 1992-06, Vol.39 (2), p.169-176 |
| issn | 0167-0115 1873-1686 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73315837 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | 3-O-Methylglucose Animals Biological and medical sciences Biological Assay Biological Transport, Active - drug effects Epidermal growth factor Epidermal Growth Factor - pharmacology Fundamental and applied biological sciences. Psychology In Vitro Techniques Intestinal Mucosa - drug effects Intestinal Mucosa - metabolism Intestinal Mucosa - ultrastructure Intestinal solute transport Intestine. Mesentery Jejunum - drug effects Jejunum - metabolism Jejunum - ultrastructure Methylglucosides - metabolism Microscopy, Electron Microvilli - drug effects Microvilli - ultrastructure Perfusion Rabbits TGFα Transforming Growth Factor alpha - analysis Transforming Growth Factor alpha - pharmacology Vertebrates: digestive system |
| title | The effect of TGFα on intestinal solute transport |
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