High-performance liquid chromatography-fluorescence assay of pyruvic acid to determine cysteine conjugate β-lyase activity: Application to S-1,2-dichlorovinyl- l-cysteine and S-2-benzothiazolyl- l-cysteine

An HPLC—fluorescence assay has been developed for the determination of the activity of rat renal cytosolic cysteine conjugate β-lyase. The method is based on isocratic HPLC separation and fluorescence detection of pyruvic acid, derivatized with o-phenylenediamine (OPD), and is shown to be rapid, spe...

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Veröffentlicht in:	Analytical biochemistry 1992-11, Vol.206 (2), p.334-343
Hauptverfasser:	Stijntjes, Gerard J., te Koppele, Johan M., Vermeulen, Nico P.E.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Animals Benzothiazoles Biological and medical sciences Carbon-Sulfur Lyases Cell Fractionation - methods Chromatography, High Pressure Liquid - methods Cysteine - analogs & derivatives Cysteine - analysis Cytosol - enzymology Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology Kidney - enzymology Kinetics Lyases Lyases - analysis Lyases - metabolism Male Mitochondria - enzymology Pyruvates - analysis Pyruvic Acid Rats Rats, Wistar Spectrometry, Fluorescence - methods
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