Formamide modified polyacrylamide gels for DNA sequencing by capillary gel electrophoresis

Compressions are occasionally found during the separation of DNA sequencing fragments, particularly in G/C‐rich regions and in gels operated at room temperature. Addition of at least 10% formamide to urea/polyacrylamide sequencing gels improves the denaturing capacity of the gel, minimizing compress...

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Veröffentlicht in:	Electrophoresis 1992, Vol.13 (1), p.484-486
Hauptverfasser:	Rocheleau, Marie J., Grey, Ronda J., Chen, Da Yong, Harke, Heather R., Dovichi, Norman J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Base Sequence Biological and medical sciences DNA - chemistry Electrophoresis, Polyacrylamide Gel - methods Formamides Fundamental and applied biological sciences. Psychology Gels - chemistry Nucleic Acid Denaturation Nucleic acids Temperature
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