Formamide modified polyacrylamide gels for DNA sequencing by capillary gel electrophoresis
Compressions are occasionally found during the separation of DNA sequencing fragments, particularly in G/C‐rich regions and in gels operated at room temperature. Addition of at least 10% formamide to urea/polyacrylamide sequencing gels improves the denaturing capacity of the gel, minimizing compress...
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| Veröffentlicht in: | Electrophoresis 1992, Vol.13 (1), p.484-486 |
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| container_end_page | 486 |
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| container_issue | 1 |
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| container_title | Electrophoresis |
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| creator | Rocheleau, Marie J. Grey, Ronda J. Chen, Da Yong Harke, Heather R. Dovichi, Norman J. |
| description | Compressions are occasionally found during the separation of DNA sequencing fragments, particularly in G/C‐rich regions and in gels operated at room temperature. Addition of at least 10% formamide to urea/polyacrylamide sequencing gels improves the denaturing capacity of the gel, minimizing compressions. Addition of 20% or more formamide decreases the separation rate, theoretical plate count, and resolution for normally migrating fragments. An optimum concentration of 10% formamide improves resolution of compressed regions without degrading the other characteristics of the gel. Operation of gels at room temperature simplifies the engineering associated with automated sequencers based on capillary gel electrophoresis. |
| doi_str_mv | 10.1002/elps.11501301102 |
| format | Article |
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Psychology</subject><subject>Gels - chemistry</subject><subject>Nucleic Acid Denaturation</subject><subject>Nucleic acids</subject><subject>Temperature</subject><issn>0173-0835</issn><issn>1522-2683</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEFv1DAQRi0EKkvhzgUpB8QtZcaOE-fAoVq6pdWqgChC4mI5zqQYnE1q7wry7_Eqq9KeOFmaed_402PsJcIJAvC35Md4gigBBSACf8QWKDnPeanEY7YArEQOSsin7FmMPwGgqIviiB1hIbFUuGDfV0PoTe9ayvqhdZ2jNhsHPxkbJj_Pb8jHrBtC9v7qNIt0u6ONdZubrJkya0bnvQnTHsrIk92GYfwxBIouPmdPOuMjvTi8x-zr6ux6-SFffzy_WJ6uc1ukrnnHm9q2UllbS9FYZWXRAXHOq8oSCKiFKoVFKQigayteKwBZ2AqV6ZqSuDhmb-a7YxhSubjVvYuWUq8NDbuoKyGwQFQJhBm0YYgxUKfH4PrUXiPovU6916nv6UyRV4fbu6an9l9g9pf2rw97E63xXTDJTbzDCqEUQpmwdzP223ma_vutPlt_-vKwRj7nXdzSn7u8Cb90WYlK6m9X53olP-Plsiz1tfgLZWyeag</recordid><startdate>1992</startdate><enddate>1992</enddate><creator>Rocheleau, Marie J.</creator><creator>Grey, Ronda J.</creator><creator>Chen, Da Yong</creator><creator>Harke, Heather R.</creator><creator>Dovichi, Norman J.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-VCH</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1992</creationdate><title>Formamide modified polyacrylamide gels for DNA sequencing by capillary gel electrophoresis</title><author>Rocheleau, Marie J. ; Grey, Ronda J. ; Chen, Da Yong ; Harke, Heather R. ; Dovichi, Norman J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4152-f2b9cd58cc953bc8c54f0e22277ce03093863c153e00fd72980054c718afb6e23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Analytical, structural and metabolic biochemistry</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>DNA - chemistry</topic><topic>Electrophoresis, Polyacrylamide Gel - methods</topic><topic>Formamides</topic><topic>Fundamental and applied biological sciences. 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| ispartof | Electrophoresis, 1992, Vol.13 (1), p.484-486 |
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| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Analytical, structural and metabolic biochemistry Base Sequence Biological and medical sciences DNA - chemistry Electrophoresis, Polyacrylamide Gel - methods Formamides Fundamental and applied biological sciences. Psychology Gels - chemistry Nucleic Acid Denaturation Nucleic acids Temperature |
| title | Formamide modified polyacrylamide gels for DNA sequencing by capillary gel electrophoresis |
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