Differential gene expression between young and senescent, quiescent WI-38 cells

To investigate age-related changes in gene expression in WI-38 cells, we isolated RNA from young and senescent, quiescent cultures and made subtracted cDNA libraries. Density-arrested cells were incubated in serum-free MCDB-104 for 3 days. RNA was then isolated and subtracted cDNA libraries were mad...

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Veröffentlicht in:	Mechanisms of ageing and development 1992-09, Vol.65 (2), p.239-255
Hauptverfasser:	Doggett, David L., Rotenberg, Mitch O., Pignolo, Robert J., Phillips, Paul D., Cristofalo, Vincent J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Aging - genetics Biological and medical sciences Cell Line Culture Media, Serum-Free Cytochrome b Group - genetics Fundamental and applied biological sciences. Psychology Gene Expression - genetics Gene Library Mitochondria - metabolism Molecular and cellular biology Molecular genetics NADH Dehydrogenase - genetics Nucleic Acid Hybridization - methods RNA
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