Impression cytology findings in bullous keratopathy
AimTo evaluate, by impression cytology (IC), the cytological features of the central area of corneal epithelial surface of patients with symptomatic bullous keratopathy (BK).DesignCross-sectional observational case series.MethodsIC of the central cornea was performed in 72 eyes of 72 patients with s...
Gespeichert in:
| Veröffentlicht in: | British journal of ophthalmology 2010-06, Vol.94 (6), p.773-776 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 776 |
|---|---|
| container_issue | 6 |
| container_start_page | 773 |
| container_title | British journal of ophthalmology |
| container_volume | 94 |
| creator | Paris, Fabiana dos Santos Gonçalves, Eliana Domingues Barros, Jeison de Nadai de Queiroz Campos, Mauro Silveira Sato, Élcio Hideo Gomes, José Álvaro Pereira |
| description | AimTo evaluate, by impression cytology (IC), the cytological features of the central area of corneal epithelial surface of patients with symptomatic bullous keratopathy (BK).DesignCross-sectional observational case series.MethodsIC of the central cornea was performed in 72 eyes of 72 patients with symptomatic BK between June 2005 and December 2006. Patients were divided into two groups according to the presence (group GC) or not of goblet cells (group NGC). The diagnosis of limbal stem cell deficiency (LSCD) was defined by the presence of one or more intact goblet cells on the corneal surface. Squamous metaplasia was classified according to Murube and Rivas.ResultsEpithelial squamous metaplasia was found in 72 (100%) eyes, along with conjunctival goblet cells in 25 (34.72%) eyes. Squamous metaplasia was grade 1 in group GC and more advanced grades in group NGC. Corneal neovascularisation was present in 62 (82.11%) eyes. Vessel measure was more than 4 mm in 11 eyes (23.4%) in group NGC and 10 eyes (40%) in group GC (p=0.559), and they were mostly subepithelial in 23 eyes (48.9%) in group NGC and 12 (48%) eyes in group GC (p=0.822).ConclusionSquamous metaplasia was the most frequent finding associated with advanced BK. The presence of goblet cells on the surface of corneas with squamous metaplasia grade 1, in a significant number of patients, suggests that limbal stem cell deficiency is a common condition associated with advanced cases of BK. |
| doi_str_mv | 10.1136/bjo.2009.158881 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_733110051</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>733110051</sourcerecordid><originalsourceid>FETCH-LOGICAL-b427t-f3cef27cc88da9866df08d7dca73eadab9444b57ea647220b4a44253ba58eae03</originalsourceid><addsrcrecordid>eNqF0F1rFDEUBuAgit1Wr72TASmCMNt8f1zKUmuhKEqt4E04yWTqbGcmazID3X9vyiwteOPVIZwnh5cXoTcErwlh8sxt45pibNZEaK3JM7QiXOqaYmWeoxXGWNWESHKEjnPelieVRL1ER8QYKTQxK8Quh10KOXdxrPx-in283VdtNzbdeJurbqzc3PdxztVdSDDFHUy_96_Qixb6HF4f5gn68en8evO5vvp6cbn5eFU7TtVUt8yHlirvtW7AaCmbFutGNR4UC9CAM5xzJ1QAyRWl2HHgnArmQOgAAbMT9H65u0vxzxzyZIcu-9D3MIYSySrGCMFYkCLf_SO3cU5jCWeJUtpwLIQp6mxRPsWcU2jtLnUDpL0l2D7UaUud9qFOu9RZfrw93J3dEJonf-ivgNMDgOyhbxOMvsuPjlLNTMHF1Yvr8hTuH_eQ7qxUTAn75WZj9a_vP7G4vrHfiv-weDds_5vyL-jjmXY</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1778940559</pqid></control><display><type>article</type><title>Impression cytology findings in bullous keratopathy</title><source>Open Access: PubMed Central</source><source>MEDLINE</source><source>BMJ Journals - NESLi2</source><creator>Paris, Fabiana dos Santos ; Gonçalves, Eliana Domingues ; Barros, Jeison de Nadai ; de Queiroz Campos, Mauro Silveira ; Sato, Élcio Hideo ; Gomes, José Álvaro Pereira</creator><creatorcontrib>Paris, Fabiana dos Santos ; Gonçalves, Eliana Domingues ; Barros, Jeison de Nadai ; de Queiroz Campos, Mauro Silveira ; Sato, Élcio Hideo ; Gomes, José Álvaro Pereira</creatorcontrib><description>AimTo evaluate, by impression cytology (IC), the cytological features of the central area of corneal epithelial surface of patients with symptomatic bullous keratopathy (BK).DesignCross-sectional observational case series.MethodsIC of the central cornea was performed in 72 eyes of 72 patients with symptomatic BK between June 2005 and December 2006. Patients were divided into two groups according to the presence (group GC) or not of goblet cells (group NGC). The diagnosis of limbal stem cell deficiency (LSCD) was defined by the presence of one or more intact goblet cells on the corneal surface. Squamous metaplasia was classified according to Murube and Rivas.ResultsEpithelial squamous metaplasia was found in 72 (100%) eyes, along with conjunctival goblet cells in 25 (34.72%) eyes. Squamous metaplasia was grade 1 in group GC and more advanced grades in group NGC. Corneal neovascularisation was present in 62 (82.11%) eyes. Vessel measure was more than 4 mm in 11 eyes (23.4%) in group NGC and 10 eyes (40%) in group GC (p=0.559), and they were mostly subepithelial in 23 eyes (48.9%) in group NGC and 12 (48%) eyes in group GC (p=0.822).ConclusionSquamous metaplasia was the most frequent finding associated with advanced BK. The presence of goblet cells on the surface of corneas with squamous metaplasia grade 1, in a significant number of patients, suggests that limbal stem cell deficiency is a common condition associated with advanced cases of BK.</description><identifier>ISSN: 0007-1161</identifier><identifier>EISSN: 1468-2079</identifier><identifier>DOI: 10.1136/bjo.2009.158881</identifier><identifier>PMID: 19965819</identifier><identifier>CODEN: BJOPAL</identifier><language>eng</language><publisher>BMA House, Tavistock Square, London, WC1H 9JR: BMJ Publishing Group Ltd</publisher><subject>Adult ; Aged ; Aged, 80 and over ; Biological and medical sciences ; bullous keratopathy ; Cellular biology ; Classification ; Contact lenses ; Cornea ; Corneal Diseases - pathology ; Corneal Neovascularization - pathology ; Cross-Sectional Studies ; Cytodiagnosis - methods ; Cytoplasm ; Diagnostic Techniques, Ophthalmological ; Diseases of cornea, anterior segment and sclera ; Endothelium ; Epithelium, Corneal - pathology ; Female ; goblet cell ; Goblet Cells - pathology ; Humans ; Impression cytology ; Limbus Corneae - pathology ; Male ; Medical sciences ; Metaplasia - pathology ; Middle Aged ; Miscellaneous ; Mucous membrane ; neovascularisation ; Ophthalmology ; Pain ; Patients ; squamous metaplasia ; Statistical analysis ; Stem cells ; Stem Cells - pathology ; Young Adult</subject><ispartof>British journal of ophthalmology, 2010-06, Vol.94 (6), p.773-776</ispartof><rights>2010, Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright: 2010 (c) 2010, Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b427t-f3cef27cc88da9866df08d7dca73eadab9444b57ea647220b4a44253ba58eae03</citedby><cites>FETCH-LOGICAL-b427t-f3cef27cc88da9866df08d7dca73eadab9444b57ea647220b4a44253ba58eae03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttp://bjo.bmj.com/content/94/6/773.full.pdf$$EPDF$$P50$$Gbmj$$H</linktopdf><linktohtml>$$Uhttp://bjo.bmj.com/content/94/6/773.full$$EHTML$$P50$$Gbmj$$H</linktohtml><link.rule.ids>114,115,314,780,784,3196,23571,27924,27925,77472,77503</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22839199$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19965819$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Paris, Fabiana dos Santos</creatorcontrib><creatorcontrib>Gonçalves, Eliana Domingues</creatorcontrib><creatorcontrib>Barros, Jeison de Nadai</creatorcontrib><creatorcontrib>de Queiroz Campos, Mauro Silveira</creatorcontrib><creatorcontrib>Sato, Élcio Hideo</creatorcontrib><creatorcontrib>Gomes, José Álvaro Pereira</creatorcontrib><title>Impression cytology findings in bullous keratopathy</title><title>British journal of ophthalmology</title><addtitle>Br J Ophthalmol</addtitle><description>AimTo evaluate, by impression cytology (IC), the cytological features of the central area of corneal epithelial surface of patients with symptomatic bullous keratopathy (BK).DesignCross-sectional observational case series.MethodsIC of the central cornea was performed in 72 eyes of 72 patients with symptomatic BK between June 2005 and December 2006. Patients were divided into two groups according to the presence (group GC) or not of goblet cells (group NGC). The diagnosis of limbal stem cell deficiency (LSCD) was defined by the presence of one or more intact goblet cells on the corneal surface. Squamous metaplasia was classified according to Murube and Rivas.ResultsEpithelial squamous metaplasia was found in 72 (100%) eyes, along with conjunctival goblet cells in 25 (34.72%) eyes. Squamous metaplasia was grade 1 in group GC and more advanced grades in group NGC. Corneal neovascularisation was present in 62 (82.11%) eyes. Vessel measure was more than 4 mm in 11 eyes (23.4%) in group NGC and 10 eyes (40%) in group GC (p=0.559), and they were mostly subepithelial in 23 eyes (48.9%) in group NGC and 12 (48%) eyes in group GC (p=0.822).ConclusionSquamous metaplasia was the most frequent finding associated with advanced BK. The presence of goblet cells on the surface of corneas with squamous metaplasia grade 1, in a significant number of patients, suggests that limbal stem cell deficiency is a common condition associated with advanced cases of BK.</description><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Biological and medical sciences</subject><subject>bullous keratopathy</subject><subject>Cellular biology</subject><subject>Classification</subject><subject>Contact lenses</subject><subject>Cornea</subject><subject>Corneal Diseases - pathology</subject><subject>Corneal Neovascularization - pathology</subject><subject>Cross-Sectional Studies</subject><subject>Cytodiagnosis - methods</subject><subject>Cytoplasm</subject><subject>Diagnostic Techniques, Ophthalmological</subject><subject>Diseases of cornea, anterior segment and sclera</subject><subject>Endothelium</subject><subject>Epithelium, Corneal - pathology</subject><subject>Female</subject><subject>goblet cell</subject><subject>Goblet Cells - pathology</subject><subject>Humans</subject><subject>Impression cytology</subject><subject>Limbus Corneae - pathology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Metaplasia - pathology</subject><subject>Middle Aged</subject><subject>Miscellaneous</subject><subject>Mucous membrane</subject><subject>neovascularisation</subject><subject>Ophthalmology</subject><subject>Pain</subject><subject>Patients</subject><subject>squamous metaplasia</subject><subject>Statistical analysis</subject><subject>Stem cells</subject><subject>Stem Cells - pathology</subject><subject>Young Adult</subject><issn>0007-1161</issn><issn>1468-2079</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNqF0F1rFDEUBuAgit1Wr72TASmCMNt8f1zKUmuhKEqt4E04yWTqbGcmazID3X9vyiwteOPVIZwnh5cXoTcErwlh8sxt45pibNZEaK3JM7QiXOqaYmWeoxXGWNWESHKEjnPelieVRL1ER8QYKTQxK8Quh10KOXdxrPx-in283VdtNzbdeJurbqzc3PdxztVdSDDFHUy_96_Qixb6HF4f5gn68en8evO5vvp6cbn5eFU7TtVUt8yHlirvtW7AaCmbFutGNR4UC9CAM5xzJ1QAyRWl2HHgnArmQOgAAbMT9H65u0vxzxzyZIcu-9D3MIYSySrGCMFYkCLf_SO3cU5jCWeJUtpwLIQp6mxRPsWcU2jtLnUDpL0l2D7UaUud9qFOu9RZfrw93J3dEJonf-ivgNMDgOyhbxOMvsuPjlLNTMHF1Yvr8hTuH_eQ7qxUTAn75WZj9a_vP7G4vrHfiv-weDds_5vyL-jjmXY</recordid><startdate>20100601</startdate><enddate>20100601</enddate><creator>Paris, Fabiana dos Santos</creator><creator>Gonçalves, Eliana Domingues</creator><creator>Barros, Jeison de Nadai</creator><creator>de Queiroz Campos, Mauro Silveira</creator><creator>Sato, Élcio Hideo</creator><creator>Gomes, José Álvaro Pereira</creator><general>BMJ Publishing Group Ltd</general><general>BMJ Publishing Group</general><general>BMJ Publishing Group LTD</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>BTHHO</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20100601</creationdate><title>Impression cytology findings in bullous keratopathy</title><author>Paris, Fabiana dos Santos ; Gonçalves, Eliana Domingues ; Barros, Jeison de Nadai ; de Queiroz Campos, Mauro Silveira ; Sato, Élcio Hideo ; Gomes, José Álvaro Pereira</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b427t-f3cef27cc88da9866df08d7dca73eadab9444b57ea647220b4a44253ba58eae03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Biological and medical sciences</topic><topic>bullous keratopathy</topic><topic>Cellular biology</topic><topic>Classification</topic><topic>Contact lenses</topic><topic>Cornea</topic><topic>Corneal Diseases - pathology</topic><topic>Corneal Neovascularization - pathology</topic><topic>Cross-Sectional Studies</topic><topic>Cytodiagnosis - methods</topic><topic>Cytoplasm</topic><topic>Diagnostic Techniques, Ophthalmological</topic><topic>Diseases of cornea, anterior segment and sclera</topic><topic>Endothelium</topic><topic>Epithelium, Corneal - pathology</topic><topic>Female</topic><topic>goblet cell</topic><topic>Goblet Cells - pathology</topic><topic>Humans</topic><topic>Impression cytology</topic><topic>Limbus Corneae - pathology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Metaplasia - pathology</topic><topic>Middle Aged</topic><topic>Miscellaneous</topic><topic>Mucous membrane</topic><topic>neovascularisation</topic><topic>Ophthalmology</topic><topic>Pain</topic><topic>Patients</topic><topic>squamous metaplasia</topic><topic>Statistical analysis</topic><topic>Stem cells</topic><topic>Stem Cells - pathology</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Paris, Fabiana dos Santos</creatorcontrib><creatorcontrib>Gonçalves, Eliana Domingues</creatorcontrib><creatorcontrib>Barros, Jeison de Nadai</creatorcontrib><creatorcontrib>de Queiroz Campos, Mauro Silveira</creatorcontrib><creatorcontrib>Sato, Élcio Hideo</creatorcontrib><creatorcontrib>Gomes, José Álvaro Pereira</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central</collection><collection>BMJ Journals</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>British journal of ophthalmology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Paris, Fabiana dos Santos</au><au>Gonçalves, Eliana Domingues</au><au>Barros, Jeison de Nadai</au><au>de Queiroz Campos, Mauro Silveira</au><au>Sato, Élcio Hideo</au><au>Gomes, José Álvaro Pereira</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Impression cytology findings in bullous keratopathy</atitle><jtitle>British journal of ophthalmology</jtitle><addtitle>Br J Ophthalmol</addtitle><date>2010-06-01</date><risdate>2010</risdate><volume>94</volume><issue>6</issue><spage>773</spage><epage>776</epage><pages>773-776</pages><issn>0007-1161</issn><eissn>1468-2079</eissn><coden>BJOPAL</coden><abstract>AimTo evaluate, by impression cytology (IC), the cytological features of the central area of corneal epithelial surface of patients with symptomatic bullous keratopathy (BK).DesignCross-sectional observational case series.MethodsIC of the central cornea was performed in 72 eyes of 72 patients with symptomatic BK between June 2005 and December 2006. Patients were divided into two groups according to the presence (group GC) or not of goblet cells (group NGC). The diagnosis of limbal stem cell deficiency (LSCD) was defined by the presence of one or more intact goblet cells on the corneal surface. Squamous metaplasia was classified according to Murube and Rivas.ResultsEpithelial squamous metaplasia was found in 72 (100%) eyes, along with conjunctival goblet cells in 25 (34.72%) eyes. Squamous metaplasia was grade 1 in group GC and more advanced grades in group NGC. Corneal neovascularisation was present in 62 (82.11%) eyes. Vessel measure was more than 4 mm in 11 eyes (23.4%) in group NGC and 10 eyes (40%) in group GC (p=0.559), and they were mostly subepithelial in 23 eyes (48.9%) in group NGC and 12 (48%) eyes in group GC (p=0.822).ConclusionSquamous metaplasia was the most frequent finding associated with advanced BK. The presence of goblet cells on the surface of corneas with squamous metaplasia grade 1, in a significant number of patients, suggests that limbal stem cell deficiency is a common condition associated with advanced cases of BK.</abstract><cop>BMA House, Tavistock Square, London, WC1H 9JR</cop><pub>BMJ Publishing Group Ltd</pub><pmid>19965819</pmid><doi>10.1136/bjo.2009.158881</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0007-1161 |
| ispartof | British journal of ophthalmology, 2010-06, Vol.94 (6), p.773-776 |
| issn | 0007-1161 1468-2079 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_733110051 |
| source | Open Access: PubMed Central; MEDLINE; BMJ Journals - NESLi2 |
| subjects | Adult Aged Aged, 80 and over Biological and medical sciences bullous keratopathy Cellular biology Classification Contact lenses Cornea Corneal Diseases - pathology Corneal Neovascularization - pathology Cross-Sectional Studies Cytodiagnosis - methods Cytoplasm Diagnostic Techniques, Ophthalmological Diseases of cornea, anterior segment and sclera Endothelium Epithelium, Corneal - pathology Female goblet cell Goblet Cells - pathology Humans Impression cytology Limbus Corneae - pathology Male Medical sciences Metaplasia - pathology Middle Aged Miscellaneous Mucous membrane neovascularisation Ophthalmology Pain Patients squamous metaplasia Statistical analysis Stem cells Stem Cells - pathology Young Adult |
| title | Impression cytology findings in bullous keratopathy |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-07T20%3A08%3A11IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Impression%20cytology%20findings%20in%20bullous%20keratopathy&rft.jtitle=British%20journal%20of%20ophthalmology&rft.au=Paris,%20Fabiana%20dos%20Santos&rft.date=2010-06-01&rft.volume=94&rft.issue=6&rft.spage=773&rft.epage=776&rft.pages=773-776&rft.issn=0007-1161&rft.eissn=1468-2079&rft.coden=BJOPAL&rft_id=info:doi/10.1136/bjo.2009.158881&rft_dat=%3Cproquest_cross%3E733110051%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1778940559&rft_id=info:pmid/19965819&rfr_iscdi=true |