Bio-electrospraying: A potentially safe technique for delivering progenitor cells
Bio-electrospraying is fast becoming an attractive tool for in situ cell delivery into scaffolds for tissue engineering applications, with several cell types been successfully electrosprayed. Bone marrow derived mesenchymal progenitor/stem cells (BMSC), which are an important cell source for tissue...
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| Veröffentlicht in: | Biotechnology and bioengineering 2010-07, Vol.106 (4), p.690-698 |
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| creator | Sahoo, Sambit Lee, Wong Cheng Goh, James Cho Hong Toh, Siew Lok |
| description | Bio-electrospraying is fast becoming an attractive tool for in situ cell delivery into scaffolds for tissue engineering applications, with several cell types been successfully electrosprayed. Bone marrow derived mesenchymal progenitor/stem cells (BMSC), which are an important cell source for tissue engineering, have not been explored in detail and the effect of electrospraying on their "stemness" is not known. This study therefore investigates the effects of electrospraying on BMSC viability, proliferation, and multilineage differentiation potential. Electrospraying a BMSC suspension at flow rate of 6 mL/h and voltages of 7.5-15 kV could successfully generate a continuous, stable and linearly directed electrospray of cells. Morphological observation, trypan blue tests and alamar blue based metabolic assays revealed about 88% of these electrosprayed cells were viable, and proliferated at rates similar to native BMSCs. However, at higher voltages, electrospraying became unstable and reduced cell viability, possibly due to electrical or thermal damage to the cells. BMSCs electrosprayed at 7.5 kV also retained their multipotency and could be successfully differentiated into adipogenic, chondrogenic, and osteogenic lineages, demonstrating similar morphology and gene expression levels as induced native BMSCs. These results indicate that bio-electrospraying could be safely used as a progenitor/stem cell delivery technique for tissue engineering and regenerative medicine applications. Biotechnol. Bioeng. 2010;106: 690-698. |
| doi_str_mv | 10.1002/bit.22734 |
| format | Article |
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Bone marrow derived mesenchymal progenitor/stem cells (BMSC), which are an important cell source for tissue engineering, have not been explored in detail and the effect of electrospraying on their "stemness" is not known. This study therefore investigates the effects of electrospraying on BMSC viability, proliferation, and multilineage differentiation potential. Electrospraying a BMSC suspension at flow rate of 6 mL/h and voltages of 7.5-15 kV could successfully generate a continuous, stable and linearly directed electrospray of cells. Morphological observation, trypan blue tests and alamar blue based metabolic assays revealed about 88% of these electrosprayed cells were viable, and proliferated at rates similar to native BMSCs. However, at higher voltages, electrospraying became unstable and reduced cell viability, possibly due to electrical or thermal damage to the cells. BMSCs electrosprayed at 7.5 kV also retained their multipotency and could be successfully differentiated into adipogenic, chondrogenic, and osteogenic lineages, demonstrating similar morphology and gene expression levels as induced native BMSCs. These results indicate that bio-electrospraying could be safely used as a progenitor/stem cell delivery technique for tissue engineering and regenerative medicine applications. Biotechnol. Bioeng. 2010;106: 690-698.</description><identifier>ISSN: 0006-3592</identifier><identifier>EISSN: 1097-0290</identifier><identifier>DOI: 10.1002/bit.22734</identifier><identifier>PMID: 20229515</identifier><identifier>CODEN: BIBIAU</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animals ; Biological and medical sciences ; Biotechnology ; Bone marrow ; bone marrow stem cells ; cell delivery ; cell seeding ; Cell Survival ; Cells ; Coloring Agents - metabolism ; Electricity ; electrospraying ; Fundamental and applied biological sciences. Psychology ; Gene expression ; Health. Pharmaceutical industry ; Industrial applications and implications. Economical aspects ; Mesenchymal Stromal Cells - cytology ; Mesenchymal Stromal Cells - physiology ; Miscellaneous ; Oxazines - metabolism ; progenitor/stem cells ; Rabbits ; Staining and Labeling - methods ; Tissue engineering ; Tissue Engineering - methods ; Tissue Scaffolds ; Tissues ; Trypan Blue - metabolism ; Xanthenes - metabolism</subject><ispartof>Biotechnology and bioengineering, 2010-07, Vol.106 (4), p.690-698</ispartof><rights>Copyright © 2010 Wiley Periodicals, Inc.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright John Wiley and Sons, Limited Jul 1, 2010</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4434-f8e53208f088ca492a976bb54a6ce8d722a755fcd2d4bcc07b08d98728227daf3</citedby><cites>FETCH-LOGICAL-c4434-f8e53208f088ca492a976bb54a6ce8d722a755fcd2d4bcc07b08d98728227daf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fbit.22734$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fbit.22734$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22879829$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20229515$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sahoo, Sambit</creatorcontrib><creatorcontrib>Lee, Wong Cheng</creatorcontrib><creatorcontrib>Goh, James Cho Hong</creatorcontrib><creatorcontrib>Toh, Siew Lok</creatorcontrib><title>Bio-electrospraying: A potentially safe technique for delivering progenitor cells</title><title>Biotechnology and bioengineering</title><addtitle>Biotechnol. Bioeng</addtitle><description>Bio-electrospraying is fast becoming an attractive tool for in situ cell delivery into scaffolds for tissue engineering applications, with several cell types been successfully electrosprayed. Bone marrow derived mesenchymal progenitor/stem cells (BMSC), which are an important cell source for tissue engineering, have not been explored in detail and the effect of electrospraying on their "stemness" is not known. This study therefore investigates the effects of electrospraying on BMSC viability, proliferation, and multilineage differentiation potential. Electrospraying a BMSC suspension at flow rate of 6 mL/h and voltages of 7.5-15 kV could successfully generate a continuous, stable and linearly directed electrospray of cells. Morphological observation, trypan blue tests and alamar blue based metabolic assays revealed about 88% of these electrosprayed cells were viable, and proliferated at rates similar to native BMSCs. However, at higher voltages, electrospraying became unstable and reduced cell viability, possibly due to electrical or thermal damage to the cells. BMSCs electrosprayed at 7.5 kV also retained their multipotency and could be successfully differentiated into adipogenic, chondrogenic, and osteogenic lineages, demonstrating similar morphology and gene expression levels as induced native BMSCs. These results indicate that bio-electrospraying could be safely used as a progenitor/stem cell delivery technique for tissue engineering and regenerative medicine applications. Biotechnol. Bioeng. 2010;106: 690-698.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Bone marrow</subject><subject>bone marrow stem cells</subject><subject>cell delivery</subject><subject>cell seeding</subject><subject>Cell Survival</subject><subject>Cells</subject><subject>Coloring Agents - metabolism</subject><subject>Electricity</subject><subject>electrospraying</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>Health. Pharmaceutical industry</subject><subject>Industrial applications and implications. Economical aspects</subject><subject>Mesenchymal Stromal Cells - cytology</subject><subject>Mesenchymal Stromal Cells - physiology</subject><subject>Miscellaneous</subject><subject>Oxazines - metabolism</subject><subject>progenitor/stem cells</subject><subject>Rabbits</subject><subject>Staining and Labeling - methods</subject><subject>Tissue engineering</subject><subject>Tissue Engineering - methods</subject><subject>Tissue Scaffolds</subject><subject>Tissues</subject><subject>Trypan Blue - metabolism</subject><subject>Xanthenes - metabolism</subject><issn>0006-3592</issn><issn>1097-0290</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10F1rFDEUBuAgil2rF_4BHQQpXkx7kkzmJN61pV9QFGmL3oVMJllTZ2fWZFbdf2_W2bYg9CokPCd58xLymsI-BWAHTRj3GUNePSEzCgpLYAqekhkA1CUXiu2QFynd5i3Kun5OdhgwpgQVM_LlKAyl65wd45CW0axDP_9YHBbLYXT9GEzXrYtkvCtGZ7_34efKFX6IReu68MvFjItlHOauD2M-ta7r0kvyzJsuuVfbdZfcnJ5cH5-Xl5_PLo4PL0tbVbwqvXSCM5AepLSmUsworJtGVKa2TrbImEEhvG1ZWzXWAjYgWyWRyfzT1ni-S_ame3OAHCuNehHSJoHp3bBKGjmngAgqy3f_ydthFfscTgtEgVxwmdGHCdlcRIrO62UMCxPXmoLetKxzy_pfy9m-2V64ahauvZd3tWbwfgtMsqbz0fQ2pAfHJCrJNskOJvc7dG79-Iv66OL67ulymghpdH_uJ0z8oWvkKPTXT2f6FK4ofjtXmmb_dvLeDNrMY05xc8WAcqCykgI4_wuV_KsN</recordid><startdate>20100701</startdate><enddate>20100701</enddate><creator>Sahoo, Sambit</creator><creator>Lee, Wong Cheng</creator><creator>Goh, James Cho Hong</creator><creator>Toh, Siew Lok</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley</general><general>Wiley Subscription Services, Inc</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20100701</creationdate><title>Bio-electrospraying: A potentially safe technique for delivering progenitor cells</title><author>Sahoo, Sambit ; Lee, Wong Cheng ; Goh, James Cho Hong ; Toh, Siew Lok</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4434-f8e53208f088ca492a976bb54a6ce8d722a755fcd2d4bcc07b08d98728227daf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Bone marrow</topic><topic>bone marrow stem cells</topic><topic>cell delivery</topic><topic>cell seeding</topic><topic>Cell Survival</topic><topic>Cells</topic><topic>Coloring Agents - metabolism</topic><topic>Electricity</topic><topic>electrospraying</topic><topic>Fundamental and applied biological sciences. 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| subjects | Animals Biological and medical sciences Biotechnology Bone marrow bone marrow stem cells cell delivery cell seeding Cell Survival Cells Coloring Agents - metabolism Electricity electrospraying Fundamental and applied biological sciences. Psychology Gene expression Health. Pharmaceutical industry Industrial applications and implications. Economical aspects Mesenchymal Stromal Cells - cytology Mesenchymal Stromal Cells - physiology Miscellaneous Oxazines - metabolism progenitor/stem cells Rabbits Staining and Labeling - methods Tissue engineering Tissue Engineering - methods Tissue Scaffolds Tissues Trypan Blue - metabolism Xanthenes - metabolism |
| title | Bio-electrospraying: A potentially safe technique for delivering progenitor cells |
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