Circulating tumour cells in clinical practice: Methods of detection and possible characterization
Circulating Tumour Cells (CTCs) can be released from the primary tumour into the bloodstream and may colonize distant organs giving rise to metastasis. The presence of CTCs in the blood has been documented more than a century ago, and in the meanwhile various methods have been described for their de...
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| Veröffentlicht in: | Methods (San Diego, Calif.) Calif.), 2010-04, Vol.50 (4), p.289-297 |
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| creator | Alunni-Fabbroni, Marianna Sandri, Maria Teresa |
| description | Circulating Tumour Cells (CTCs) can be released from the primary tumour into the bloodstream and may colonize distant organs giving rise to metastasis. The presence of CTCs in the blood has been documented more than a century ago, and in the meanwhile various methods have been described for their detection. Most of them require an initial enrichment step, since CTCs are a very rare event. The different technologies and also the differences among the screened populations make the clinical significance of CTCs detection difficult to interprete. Here we will review the different assays up to now available for CTC detection and analysis. Moreover, we will focus on the relevance of the clinical data, generated so far and based on the CTCs analysis. Since the vast majority of data have been produced in breast cancer patients, the review will focus especially on this malignancy. |
| doi_str_mv | 10.1016/j.ymeth.2010.01.027 |
| format | Article |
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The presence of CTCs in the blood has been documented more than a century ago, and in the meanwhile various methods have been described for their detection. Most of them require an initial enrichment step, since CTCs are a very rare event. The different technologies and also the differences among the screened populations make the clinical significance of CTCs detection difficult to interprete. Here we will review the different assays up to now available for CTC detection and analysis. Moreover, we will focus on the relevance of the clinical data, generated so far and based on the CTCs analysis. 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Since the vast majority of data have been produced in breast cancer patients, the review will focus especially on this malignancy.</description><subject>Apoptosis Regulatory Proteins - analysis</subject><subject>Biomarkers, Tumor - analysis</subject><subject>Breast Neoplasms - blood</subject><subject>Breast Neoplasms - pathology</subject><subject>Cell characterization</subject><subject>Cell Separation - methods</subject><subject>Centrifugation, Density Gradient - methods</subject><subject>Circulating tumour cell</subject><subject>Comparative Genomic Hybridization</subject><subject>Detection method</subject><subject>Enrichment method</subject><subject>Female</subject><subject>Humans</subject><subject>Immunomagnetic separation</subject><subject>Keratins - analysis</subject><subject>Leukocyte Common Antigens - analysis</subject><subject>Lymphatic Metastasis</subject><subject>Minimal residual disease</subject><subject>Neoplasm Metastasis</subject><subject>Neoplastic Cells, Circulating - chemistry</subject><subject>Neoplastic Cells, Circulating - pathology</subject><subject>Prognosis</subject><subject>Real time PCR</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RT-PCR</subject><issn>1046-2023</issn><issn>1095-9130</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtOwzAQRS0EoqXwBUjIO1YJYzvNA4kFqnhJRWxgbTnOhLpK4mInSOXrcWhhyWpG43tnfA8h5wxiBiy9WsfbFvtVzCFMgMXAswMyZVDMo4IJOBz7JI04cDEhJ96vAYDxLD8mk2BhaSL4lKiFcXpoVG-6d9oPrR0c1dg0npqO6sZ0RquGbpzSvdF4TZ_DRVt5amtaYY9hajuquopurPembJDqlRrV6MyXGl9PyVGtGo9n-zojb_d3r4vHaPny8LS4XUZazPM-yuYJ1yXwUilWiSypy5KpXLMsy6FIeBmiZEXCUgG6KOoQME-qgiPUWmGhSi5m5HK3d-Psx4C-l63xYxTVoR28zIRgwHOeBqXYKbULn3ZYy40zrXJbyUCOaOVa_qCVI1oJTAa0wXWx3z-ULVZ_nl-WQXCzE2BI-WnQSa8Ndhor4wIoWVnz74FvusmMMQ</recordid><startdate>20100401</startdate><enddate>20100401</enddate><creator>Alunni-Fabbroni, Marianna</creator><creator>Sandri, Maria Teresa</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20100401</creationdate><title>Circulating tumour cells in clinical practice: Methods of detection and possible characterization</title><author>Alunni-Fabbroni, Marianna ; Sandri, Maria Teresa</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c358t-7542cb02baa1d374fbb1a8c17780942b0467941630c99f91384d92e0fcae9ab23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Apoptosis Regulatory Proteins - analysis</topic><topic>Biomarkers, Tumor - analysis</topic><topic>Breast Neoplasms - blood</topic><topic>Breast Neoplasms - pathology</topic><topic>Cell characterization</topic><topic>Cell Separation - methods</topic><topic>Centrifugation, Density Gradient - methods</topic><topic>Circulating tumour cell</topic><topic>Comparative Genomic Hybridization</topic><topic>Detection method</topic><topic>Enrichment method</topic><topic>Female</topic><topic>Humans</topic><topic>Immunomagnetic separation</topic><topic>Keratins - analysis</topic><topic>Leukocyte Common Antigens - analysis</topic><topic>Lymphatic Metastasis</topic><topic>Minimal residual disease</topic><topic>Neoplasm Metastasis</topic><topic>Neoplastic Cells, Circulating - chemistry</topic><topic>Neoplastic Cells, Circulating - pathology</topic><topic>Prognosis</topic><topic>Real time PCR</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RT-PCR</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Alunni-Fabbroni, Marianna</creatorcontrib><creatorcontrib>Sandri, Maria Teresa</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Methods (San Diego, Calif.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Alunni-Fabbroni, Marianna</au><au>Sandri, Maria Teresa</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Circulating tumour cells in clinical practice: Methods of detection and possible characterization</atitle><jtitle>Methods (San Diego, Calif.)</jtitle><addtitle>Methods</addtitle><date>2010-04-01</date><risdate>2010</risdate><volume>50</volume><issue>4</issue><spage>289</spage><epage>297</epage><pages>289-297</pages><issn>1046-2023</issn><eissn>1095-9130</eissn><abstract>Circulating Tumour Cells (CTCs) can be released from the primary tumour into the bloodstream and may colonize distant organs giving rise to metastasis. The presence of CTCs in the blood has been documented more than a century ago, and in the meanwhile various methods have been described for their detection. Most of them require an initial enrichment step, since CTCs are a very rare event. The different technologies and also the differences among the screened populations make the clinical significance of CTCs detection difficult to interprete. Here we will review the different assays up to now available for CTC detection and analysis. Moreover, we will focus on the relevance of the clinical data, generated so far and based on the CTCs analysis. Since the vast majority of data have been produced in breast cancer patients, the review will focus especially on this malignancy.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>20116432</pmid><doi>10.1016/j.ymeth.2010.01.027</doi><tpages>9</tpages></addata></record> |
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| ispartof | Methods (San Diego, Calif.), 2010-04, Vol.50 (4), p.289-297 |
| issn | 1046-2023 1095-9130 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Apoptosis Regulatory Proteins - analysis Biomarkers, Tumor - analysis Breast Neoplasms - blood Breast Neoplasms - pathology Cell characterization Cell Separation - methods Centrifugation, Density Gradient - methods Circulating tumour cell Comparative Genomic Hybridization Detection method Enrichment method Female Humans Immunomagnetic separation Keratins - analysis Leukocyte Common Antigens - analysis Lymphatic Metastasis Minimal residual disease Neoplasm Metastasis Neoplastic Cells, Circulating - chemistry Neoplastic Cells, Circulating - pathology Prognosis Real time PCR Reverse Transcriptase Polymerase Chain Reaction RT-PCR |
| title | Circulating tumour cells in clinical practice: Methods of detection and possible characterization |
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