Lipocalin-Type Prostaglandin D Synthase and Egg White Cystatin React with IgE Antibodies from Children with Egg Allergy
Ovalbumin, ovomucoid, ovotransferrin, lysozyme, and ovomucin are known to be major allergens found in egg white. Egg white protein is composed of over 30 proteins; many of which have neither been identified nor their allergenicities characterized. This study set out to analyze whether unknown protei...
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| Veröffentlicht in: | Allergology international 2010, Vol.59 (2), p.175-183 |
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| creator | Suzuki, Makiko Fujii, Hidehiko Fujigaki, Hidetsugu Shinoda, Shinji Takahashi, Kanako Saito, Kuniaki Wada, Hisayasu Kimoto, Masumi Kondo, Naomi Seishima, Mitsuru |
| description | Ovalbumin, ovomucoid, ovotransferrin, lysozyme, and ovomucin are known to be major allergens found in egg white. Egg white protein is composed of over 30 proteins; many of which have neither been identified nor their allergenicities characterized. This study set out to analyze whether unknown proteins that bind to IgE antibodies in serum from patients with egg allergy exist in egg white.
Diluted egg white proteins were separated by 2-dimensional (2-D) gel electrophoresis. Immunolabeling was performed on individual patient sera from 19 child patients with egg white allergy and 11 negative control subjects. Spots of egg white proteins that bound to the patients' IgE were identified by mass spectrometry-based proteomics.
Egg white proteins were separated into 63 spots. Twenty-five of the 63 reacted with egg allergy patients' sera, and 10 of the 25 reactive spots showed IgE-reactivity to controls as well. Specific bindings to the IgE from egg allergy patients were found in 15 spots; one of which was confirmed as ovotransferrin. Among the other 14 protein spots, egg white cystatin and lipocalin-type prostaglandin D synthase (L-PGDS) were newly identified proteins that reacted with IgE in patients with egg allergy.
We demonstrated that L-PGDS and cystatin reacted with serum IgE in patients with egg allergy. Our proteomics-based analysis in egg white gives a comprehensive map of proteins bound with IgE and should assist in enabling more accurate diagnoses and recommendations of desensitizing treatments for individual patients. |
| doi_str_mv | 10.2332/allergolint.09-OA-0121 |
| format | Article |
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Diluted egg white proteins were separated by 2-dimensional (2-D) gel electrophoresis. Immunolabeling was performed on individual patient sera from 19 child patients with egg white allergy and 11 negative control subjects. Spots of egg white proteins that bound to the patients' IgE were identified by mass spectrometry-based proteomics.
Egg white proteins were separated into 63 spots. Twenty-five of the 63 reacted with egg allergy patients' sera, and 10 of the 25 reactive spots showed IgE-reactivity to controls as well. Specific bindings to the IgE from egg allergy patients were found in 15 spots; one of which was confirmed as ovotransferrin. Among the other 14 protein spots, egg white cystatin and lipocalin-type prostaglandin D synthase (L-PGDS) were newly identified proteins that reacted with IgE in patients with egg allergy.
We demonstrated that L-PGDS and cystatin reacted with serum IgE in patients with egg allergy. Our proteomics-based analysis in egg white gives a comprehensive map of proteins bound with IgE and should assist in enabling more accurate diagnoses and recommendations of desensitizing treatments for individual patients.</description><identifier>ISSN: 1323-8930</identifier><identifier>EISSN: 1440-1592</identifier><identifier>DOI: 10.2332/allergolint.09-OA-0121</identifier><identifier>PMID: 20179418</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Allergens - chemistry ; Allergens - immunology ; Blotting, Western ; Child ; Child, Preschool ; Cloning, Molecular ; cystatin ; Cystatins - chemistry ; Cystatins - immunology ; Egg Hypersensitivity - blood ; Egg Hypersensitivity - immunology ; Egg Hypersensitivity - physiopathology ; Egg Proteins - chemistry ; Egg Proteins - immunology ; Female ; food allergy ; hen’s egg white ; Humans ; Immunoglobulin E - blood ; Immunoglobulin E - immunology ; Infant ; Intramolecular Oxidoreductases - chemistry ; Intramolecular Oxidoreductases - genetics ; Intramolecular Oxidoreductases - immunology ; Intramolecular Oxidoreductases - metabolism ; LC-MS/MS ; lipocalin-type prostaglandin D synthase ; Lipocalins - chemistry ; Lipocalins - genetics ; Lipocalins - immunology ; Lipocalins - metabolism ; Male ; Mass Spectrometry ; Proteomics ; Recombinant Fusion Proteins - genetics</subject><ispartof>Allergology international, 2010, Vol.59 (2), p.175-183</ispartof><rights>2010 Japanese Society of Allergology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4201-69bf1684009f2f2816a773e845c877c8e7e68498172df0c6a1ba9695465188113</citedby><cites>FETCH-LOGICAL-c4201-69bf1684009f2f2816a773e845c877c8e7e68498172df0c6a1ba9695465188113</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,860,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20179418$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Suzuki, Makiko</creatorcontrib><creatorcontrib>Fujii, Hidehiko</creatorcontrib><creatorcontrib>Fujigaki, Hidetsugu</creatorcontrib><creatorcontrib>Shinoda, Shinji</creatorcontrib><creatorcontrib>Takahashi, Kanako</creatorcontrib><creatorcontrib>Saito, Kuniaki</creatorcontrib><creatorcontrib>Wada, Hisayasu</creatorcontrib><creatorcontrib>Kimoto, Masumi</creatorcontrib><creatorcontrib>Kondo, Naomi</creatorcontrib><creatorcontrib>Seishima, Mitsuru</creatorcontrib><title>Lipocalin-Type Prostaglandin D Synthase and Egg White Cystatin React with IgE Antibodies from Children with Egg Allergy</title><title>Allergology international</title><addtitle>Allergol Int</addtitle><description>Ovalbumin, ovomucoid, ovotransferrin, lysozyme, and ovomucin are known to be major allergens found in egg white. Egg white protein is composed of over 30 proteins; many of which have neither been identified nor their allergenicities characterized. This study set out to analyze whether unknown proteins that bind to IgE antibodies in serum from patients with egg allergy exist in egg white.
Diluted egg white proteins were separated by 2-dimensional (2-D) gel electrophoresis. Immunolabeling was performed on individual patient sera from 19 child patients with egg white allergy and 11 negative control subjects. Spots of egg white proteins that bound to the patients' IgE were identified by mass spectrometry-based proteomics.
Egg white proteins were separated into 63 spots. Twenty-five of the 63 reacted with egg allergy patients' sera, and 10 of the 25 reactive spots showed IgE-reactivity to controls as well. Specific bindings to the IgE from egg allergy patients were found in 15 spots; one of which was confirmed as ovotransferrin. Among the other 14 protein spots, egg white cystatin and lipocalin-type prostaglandin D synthase (L-PGDS) were newly identified proteins that reacted with IgE in patients with egg allergy.
We demonstrated that L-PGDS and cystatin reacted with serum IgE in patients with egg allergy. Our proteomics-based analysis in egg white gives a comprehensive map of proteins bound with IgE and should assist in enabling more accurate diagnoses and recommendations of desensitizing treatments for individual patients.</description><subject>Allergens - chemistry</subject><subject>Allergens - immunology</subject><subject>Blotting, Western</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Cloning, Molecular</subject><subject>cystatin</subject><subject>Cystatins - chemistry</subject><subject>Cystatins - immunology</subject><subject>Egg Hypersensitivity - blood</subject><subject>Egg Hypersensitivity - immunology</subject><subject>Egg Hypersensitivity - physiopathology</subject><subject>Egg Proteins - chemistry</subject><subject>Egg Proteins - immunology</subject><subject>Female</subject><subject>food allergy</subject><subject>hen’s egg white</subject><subject>Humans</subject><subject>Immunoglobulin E - blood</subject><subject>Immunoglobulin E - immunology</subject><subject>Infant</subject><subject>Intramolecular Oxidoreductases - chemistry</subject><subject>Intramolecular Oxidoreductases - genetics</subject><subject>Intramolecular Oxidoreductases - immunology</subject><subject>Intramolecular Oxidoreductases - metabolism</subject><subject>LC-MS/MS</subject><subject>lipocalin-type prostaglandin D synthase</subject><subject>Lipocalins - chemistry</subject><subject>Lipocalins - genetics</subject><subject>Lipocalins - immunology</subject><subject>Lipocalins - metabolism</subject><subject>Male</subject><subject>Mass Spectrometry</subject><subject>Proteomics</subject><subject>Recombinant Fusion Proteins - genetics</subject><issn>1323-8930</issn><issn>1440-1592</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkElP6zAURi30EPNfQN69VYqHNLZ3RH1lkCoVMYil5To3qVGa9NkuKP8elwJix8qWfb47HITOKRkxztmFaVvwTd-6Lo6IyuZlRiije-iI5jnJ6FixP-nOGc-k4uQQHYfwQhIilDhAh4xQoXIqj9DbzK17a1Kd7HFYA77zfYimaU1XuQ7_ww9DF5cmAE4PeNo0-HnpIuDJkKiYiHswNuI3F5f4tpnisotu0VcOAq59v8KTpWsrD92O2ObLj8GHU7RfmzbA2ed5gp6upo-Tm2w2v76dlLPM5mnIrFCLmhYyJ0TVrGaSFkYIDjIfWymElSAg_SpJBatqYgtDF0YVapwXYyolpfwE_d3VXfv-_wZC1CsXLLRpQeg3QQvOiaKFEoksdqRNCoKHWq-9Wxk_aEr01rn-4VwTpeel3jpPwfPPFpvFCqrv2JfkBFzuAEiLvjrwOlgHnYXKebBRV737rcc7I3iWvw</recordid><startdate>2010</startdate><enddate>2010</enddate><creator>Suzuki, Makiko</creator><creator>Fujii, Hidehiko</creator><creator>Fujigaki, Hidetsugu</creator><creator>Shinoda, Shinji</creator><creator>Takahashi, Kanako</creator><creator>Saito, Kuniaki</creator><creator>Wada, Hisayasu</creator><creator>Kimoto, Masumi</creator><creator>Kondo, Naomi</creator><creator>Seishima, Mitsuru</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2010</creationdate><title>Lipocalin-Type Prostaglandin D Synthase and Egg White Cystatin React with IgE Antibodies from Children with Egg Allergy</title><author>Suzuki, Makiko ; Fujii, Hidehiko ; Fujigaki, Hidetsugu ; Shinoda, Shinji ; Takahashi, Kanako ; Saito, Kuniaki ; Wada, Hisayasu ; Kimoto, Masumi ; Kondo, Naomi ; Seishima, Mitsuru</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4201-69bf1684009f2f2816a773e845c877c8e7e68498172df0c6a1ba9695465188113</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Allergens - chemistry</topic><topic>Allergens - immunology</topic><topic>Blotting, Western</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Cloning, Molecular</topic><topic>cystatin</topic><topic>Cystatins - chemistry</topic><topic>Cystatins - immunology</topic><topic>Egg Hypersensitivity - blood</topic><topic>Egg Hypersensitivity - immunology</topic><topic>Egg Hypersensitivity - physiopathology</topic><topic>Egg Proteins - chemistry</topic><topic>Egg Proteins - immunology</topic><topic>Female</topic><topic>food allergy</topic><topic>hen’s egg white</topic><topic>Humans</topic><topic>Immunoglobulin E - blood</topic><topic>Immunoglobulin E - immunology</topic><topic>Infant</topic><topic>Intramolecular Oxidoreductases - chemistry</topic><topic>Intramolecular Oxidoreductases - genetics</topic><topic>Intramolecular Oxidoreductases - immunology</topic><topic>Intramolecular Oxidoreductases - metabolism</topic><topic>LC-MS/MS</topic><topic>lipocalin-type prostaglandin D synthase</topic><topic>Lipocalins - chemistry</topic><topic>Lipocalins - genetics</topic><topic>Lipocalins - immunology</topic><topic>Lipocalins - metabolism</topic><topic>Male</topic><topic>Mass Spectrometry</topic><topic>Proteomics</topic><topic>Recombinant Fusion Proteins - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Suzuki, Makiko</creatorcontrib><creatorcontrib>Fujii, Hidehiko</creatorcontrib><creatorcontrib>Fujigaki, Hidetsugu</creatorcontrib><creatorcontrib>Shinoda, Shinji</creatorcontrib><creatorcontrib>Takahashi, Kanako</creatorcontrib><creatorcontrib>Saito, Kuniaki</creatorcontrib><creatorcontrib>Wada, Hisayasu</creatorcontrib><creatorcontrib>Kimoto, Masumi</creatorcontrib><creatorcontrib>Kondo, Naomi</creatorcontrib><creatorcontrib>Seishima, Mitsuru</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Allergology international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Suzuki, Makiko</au><au>Fujii, Hidehiko</au><au>Fujigaki, Hidetsugu</au><au>Shinoda, Shinji</au><au>Takahashi, Kanako</au><au>Saito, Kuniaki</au><au>Wada, Hisayasu</au><au>Kimoto, Masumi</au><au>Kondo, Naomi</au><au>Seishima, Mitsuru</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Lipocalin-Type Prostaglandin D Synthase and Egg White Cystatin React with IgE Antibodies from Children with Egg Allergy</atitle><jtitle>Allergology international</jtitle><addtitle>Allergol Int</addtitle><date>2010</date><risdate>2010</risdate><volume>59</volume><issue>2</issue><spage>175</spage><epage>183</epage><pages>175-183</pages><issn>1323-8930</issn><eissn>1440-1592</eissn><abstract>Ovalbumin, ovomucoid, ovotransferrin, lysozyme, and ovomucin are known to be major allergens found in egg white. Egg white protein is composed of over 30 proteins; many of which have neither been identified nor their allergenicities characterized. This study set out to analyze whether unknown proteins that bind to IgE antibodies in serum from patients with egg allergy exist in egg white.
Diluted egg white proteins were separated by 2-dimensional (2-D) gel electrophoresis. Immunolabeling was performed on individual patient sera from 19 child patients with egg white allergy and 11 negative control subjects. Spots of egg white proteins that bound to the patients' IgE were identified by mass spectrometry-based proteomics.
Egg white proteins were separated into 63 spots. Twenty-five of the 63 reacted with egg allergy patients' sera, and 10 of the 25 reactive spots showed IgE-reactivity to controls as well. Specific bindings to the IgE from egg allergy patients were found in 15 spots; one of which was confirmed as ovotransferrin. Among the other 14 protein spots, egg white cystatin and lipocalin-type prostaglandin D synthase (L-PGDS) were newly identified proteins that reacted with IgE in patients with egg allergy.
We demonstrated that L-PGDS and cystatin reacted with serum IgE in patients with egg allergy. Our proteomics-based analysis in egg white gives a comprehensive map of proteins bound with IgE and should assist in enabling more accurate diagnoses and recommendations of desensitizing treatments for individual patients.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>20179418</pmid><doi>10.2332/allergolint.09-OA-0121</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Allergens - chemistry Allergens - immunology Blotting, Western Child Child, Preschool Cloning, Molecular cystatin Cystatins - chemistry Cystatins - immunology Egg Hypersensitivity - blood Egg Hypersensitivity - immunology Egg Hypersensitivity - physiopathology Egg Proteins - chemistry Egg Proteins - immunology Female food allergy hen’s egg white Humans Immunoglobulin E - blood Immunoglobulin E - immunology Infant Intramolecular Oxidoreductases - chemistry Intramolecular Oxidoreductases - genetics Intramolecular Oxidoreductases - immunology Intramolecular Oxidoreductases - metabolism LC-MS/MS lipocalin-type prostaglandin D synthase Lipocalins - chemistry Lipocalins - genetics Lipocalins - immunology Lipocalins - metabolism Male Mass Spectrometry Proteomics Recombinant Fusion Proteins - genetics |
| title | Lipocalin-Type Prostaglandin D Synthase and Egg White Cystatin React with IgE Antibodies from Children with Egg Allergy |
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