The regulation of arachidonic acid metabolism in human first trimester trophoblast by cyclic AMP
Human trophoblast cells are known to release a range of arachidonic acid metabolites into culture medium, including cyclo-oxygenase, lipoxygenase and epoxygenase products. In this study we investigated the effects of dibutyryl cyclic AMP (db cAMP) on arachidonic acid metabolism in human first trimes...
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| Veröffentlicht in: | Prostaglandins & other lipid mediators 2003-04, Vol.71 (1), p.43-53 |
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| creator | Zosmer, A. Elder, M.G. Sullivan, M.H.F. |
| description | Human trophoblast cells are known to release a range of arachidonic acid metabolites into culture medium, including cyclo-oxygenase, lipoxygenase and epoxygenase products. In this study we investigated the effects of dibutyryl cyclic AMP (db cAMP) on arachidonic acid metabolism in human first trimester trophoblast cells, and also determined the distribution of metabolites between intracellular and extracellular compartments.
db cAMP increased intracellular levels of radioactivity within 2
min, and extracellular levels of radioactivity were increased after 30
min. These changes were reflected in increased levels of arachidonic acid metabolites in both compartments, indicating that arachidonic acid was metabolised.
db cAMP increased intracellular levels of 5,6-epoxyeicosatrienoic acid (5,6-EpETrE) within 2
min of addition to cultured cells. No changes were detected after 5–10
min, but substantial changes were found 30
min after the addition of db cAMP. The dihydroxyeicosatrienoic acid (DiHETrE) breakdown products also increased with similar kinetics. In contrast, levels of 14,15-EpETrE increased after 5–10
min.
Extracellular levels of both EpETrEs and DiHETrEs increased only after 30
min, indicating that there was a differential distribution of arachidonic acid metabolites. |
| doi_str_mv | 10.1016/S0090-6980(03)00005-4 |
| format | Article |
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db cAMP increased intracellular levels of radioactivity within 2
min, and extracellular levels of radioactivity were increased after 30
min. These changes were reflected in increased levels of arachidonic acid metabolites in both compartments, indicating that arachidonic acid was metabolised.
db cAMP increased intracellular levels of 5,6-epoxyeicosatrienoic acid (5,6-EpETrE) within 2
min of addition to cultured cells. No changes were detected after 5–10
min, but substantial changes were found 30
min after the addition of db cAMP. The dihydroxyeicosatrienoic acid (DiHETrE) breakdown products also increased with similar kinetics. In contrast, levels of 14,15-EpETrE increased after 5–10
min.
Extracellular levels of both EpETrEs and DiHETrEs increased only after 30
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db cAMP increased intracellular levels of radioactivity within 2
min, and extracellular levels of radioactivity were increased after 30
min. These changes were reflected in increased levels of arachidonic acid metabolites in both compartments, indicating that arachidonic acid was metabolised.
db cAMP increased intracellular levels of 5,6-epoxyeicosatrienoic acid (5,6-EpETrE) within 2
min of addition to cultured cells. No changes were detected after 5–10
min, but substantial changes were found 30
min after the addition of db cAMP. The dihydroxyeicosatrienoic acid (DiHETrE) breakdown products also increased with similar kinetics. In contrast, levels of 14,15-EpETrE increased after 5–10
min.
Extracellular levels of both EpETrEs and DiHETrEs increased only after 30
min, indicating that there was a differential distribution of arachidonic acid metabolites.</description><subject>8,11,14-Eicosatrienoic Acid - analogs & derivatives</subject><subject>8,11,14-Eicosatrienoic Acid - analysis</subject><subject>8,11,14-Eicosatrienoic Acid - metabolism</subject><subject>Arachidonic acid</subject><subject>Arachidonic Acid - metabolism</subject><subject>Bucladesine - pharmacology</subject><subject>Cells, Cultured</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Dibutyryl cyclic AMP</subject><subject>Female</subject><subject>Humans</subject><subject>Pregnancy</subject><subject>Pregnancy Trimester, First</subject><subject>Tritium</subject><subject>Trophoblast</subject><subject>Trophoblasts - drug effects</subject><subject>Trophoblasts - metabolism</subject><issn>1098-8823</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE9P3DAQxX0oYmHbj9DKpwoOoePYzjonhBAFJCqQSs-u_0y6rpJ4sROk_fb1sitxrC8zGr3nmfcj5DODCwas-fYToIWqaRWcAT-H8mQlPpATBq2qlKr5gpzm_BegBsbgmCxYvRKtbPkJ-f28Rprwz9ybKcSRxo6aZNw6-DgGR40Lng44GRv7kAcaRrqeBzPSLqQ80SmFAfOEqXRxs462N2Vqt9RtXV_sVz-ePpKjzvQZPx3qkvz6fvN8fVc9PN7eX189VI43bKqYZc6glNhyWIkaWy-k8WKFTnYS69Ja5Wrhm0b5TvkShAtpjVTO2ppbx5fk6_7fTYovczlKDyE77HszYpyzXnEOqhaiCOVe6FLMOWGnNyWFSVvNQO9w6jeceodTA9dvOPXO9-WwYLYD-nfXgWURXO4FWGK-Bkw6u4CjQx8Sukn7GP6z4h-V7IgE</recordid><startdate>20030401</startdate><enddate>20030401</enddate><creator>Zosmer, A.</creator><creator>Elder, M.G.</creator><creator>Sullivan, M.H.F.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20030401</creationdate><title>The regulation of arachidonic acid metabolism in human first trimester trophoblast by cyclic AMP</title><author>Zosmer, A. ; Elder, M.G. ; Sullivan, M.H.F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c361t-1b1cae55e930742e9d45ad47ec5f5e2ad4b8c24d668df8d201345ba58cbb23bc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>8,11,14-Eicosatrienoic Acid - analogs & derivatives</topic><topic>8,11,14-Eicosatrienoic Acid - analysis</topic><topic>8,11,14-Eicosatrienoic Acid - metabolism</topic><topic>Arachidonic acid</topic><topic>Arachidonic Acid - metabolism</topic><topic>Bucladesine - pharmacology</topic><topic>Cells, Cultured</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Dibutyryl cyclic AMP</topic><topic>Female</topic><topic>Humans</topic><topic>Pregnancy</topic><topic>Pregnancy Trimester, First</topic><topic>Tritium</topic><topic>Trophoblast</topic><topic>Trophoblasts - drug effects</topic><topic>Trophoblasts - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zosmer, A.</creatorcontrib><creatorcontrib>Elder, M.G.</creatorcontrib><creatorcontrib>Sullivan, M.H.F.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Prostaglandins & other lipid mediators</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zosmer, A.</au><au>Elder, M.G.</au><au>Sullivan, M.H.F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The regulation of arachidonic acid metabolism in human first trimester trophoblast by cyclic AMP</atitle><jtitle>Prostaglandins & other lipid mediators</jtitle><addtitle>Prostaglandins Other Lipid Mediat</addtitle><date>2003-04-01</date><risdate>2003</risdate><volume>71</volume><issue>1</issue><spage>43</spage><epage>53</epage><pages>43-53</pages><issn>1098-8823</issn><abstract>Human trophoblast cells are known to release a range of arachidonic acid metabolites into culture medium, including cyclo-oxygenase, lipoxygenase and epoxygenase products. In this study we investigated the effects of dibutyryl cyclic AMP (db cAMP) on arachidonic acid metabolism in human first trimester trophoblast cells, and also determined the distribution of metabolites between intracellular and extracellular compartments.
db cAMP increased intracellular levels of radioactivity within 2
min, and extracellular levels of radioactivity were increased after 30
min. These changes were reflected in increased levels of arachidonic acid metabolites in both compartments, indicating that arachidonic acid was metabolised.
db cAMP increased intracellular levels of 5,6-epoxyeicosatrienoic acid (5,6-EpETrE) within 2
min of addition to cultured cells. No changes were detected after 5–10
min, but substantial changes were found 30
min after the addition of db cAMP. The dihydroxyeicosatrienoic acid (DiHETrE) breakdown products also increased with similar kinetics. In contrast, levels of 14,15-EpETrE increased after 5–10
min.
Extracellular levels of both EpETrEs and DiHETrEs increased only after 30
min, indicating that there was a differential distribution of arachidonic acid metabolites.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>12749593</pmid><doi>10.1016/S0090-6980(03)00005-4</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1098-8823 |
| ispartof | Prostaglandins & other lipid mediators, 2003-04, Vol.71 (1), p.43-53 |
| issn | 1098-8823 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | 8,11,14-Eicosatrienoic Acid - analogs & derivatives 8,11,14-Eicosatrienoic Acid - analysis 8,11,14-Eicosatrienoic Acid - metabolism Arachidonic acid Arachidonic Acid - metabolism Bucladesine - pharmacology Cells, Cultured Chromatography, High Pressure Liquid Dibutyryl cyclic AMP Female Humans Pregnancy Pregnancy Trimester, First Tritium Trophoblast Trophoblasts - drug effects Trophoblasts - metabolism |
| title | The regulation of arachidonic acid metabolism in human first trimester trophoblast by cyclic AMP |
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