Stable transfection of calbindin-D28k into the GH3 cell line alters calcium currents and intracellular calcium homeostasis
Previous work demonstrating the presence and differential distribution of Ca(2+)-binding proteins in the CNS has led to the proposal that cytosolic proteins, such as calbindin-D28k (CB), may play a pivotal role in neurons. We have used a retrovirus containing the full-length cDNA for CB to transfect...
Gespeichert in:
| Veröffentlicht in: | Neuron (Cambridge, Mass.) Mass.), 1992-11, Vol.9 (5), p.943-954 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 954 |
|---|---|
| container_issue | 5 |
| container_start_page | 943 |
| container_title | Neuron (Cambridge, Mass.) |
| container_volume | 9 |
| creator | LLEDO, P.-M SOMASUNDARAM, B MORTON, A. J EMSON, P. C MASON, W. T |
| description | Previous work demonstrating the presence and differential distribution of Ca(2+)-binding proteins in the CNS has led to the proposal that cytosolic proteins, such as calbindin-D28k (CB), may play a pivotal role in neurons. We have used a retrovirus containing the full-length cDNA for CB to transfect the pituitary tumor cell line GH3, to generate CB-expressing GH3 cells and to investigate whether ionic channel activities as well as the concentration of intracellular free Ca2+ ([Ca2+]i) homeostasis could be altered by the presence of this Ca(2+)-binding protein. We show that CB-transfected GH3 cells exhibited lower Ca2+ entry through voltage-dependent Ca2+ channels and were better able to reduce [Ca2+]i transients evoked by voltage depolarizations than the wild-type parent cell line. These observations provide a mechanism by which CB may protect tissues against Ca(2+)-mediated excitotoxicity. |
| doi_str_mv | 10.1016/0896-6273(92)90246-A |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_73306783</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>73306783</sourcerecordid><originalsourceid>FETCH-LOGICAL-c331t-7c81a2d3061c359138afbc746fd17fb8907bff2c75446b3f80456945fe3aed713</originalsourceid><addsrcrecordid>eNpFkEtPxCAUhYnRjOPjH2jCwhhdVKFQHsvJ-BgTExfqmlAKEW2pAl3or7d1JuPqLs53Tm4-AE4wusIIs2skJCtYycmFLC8lKikrFjtgjpHkBcVS7oL5FtkHBym9I4RpJfEMzDAppWB0Dn6es65bC3PUITlrsu8D7B00uq19aHwobkrxAX3IPcxvFt6vCDS2bWHrg4W6zTamCTZ-6KAZYrQhJ6hDM1WintCh1XGLvPWd7VPWyacjsOd0m-zx5h6C17vbl-WqeHy6f1guHgtDCM4FNwLrsiGIYUPG74nQrjacMtdg7mohEa-dKw2vKGU1cQLRiklaOUu0bTgmh-B8vfsZ-6_Bpqw6n6bHdLD9kBQn4zYXZATpGjSxTylapz6j73T8VhipSbmafKrJp5Kl-lOuFmPtdLM_1J1t_ktrx2N-tsl1GjW40bTxaYtRWglUUfIL3lCKRg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>73306783</pqid></control><display><type>article</type><title>Stable transfection of calbindin-D28k into the GH3 cell line alters calcium currents and intracellular calcium homeostasis</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>LLEDO, P.-M ; SOMASUNDARAM, B ; MORTON, A. J ; EMSON, P. C ; MASON, W. T</creator><creatorcontrib>LLEDO, P.-M ; SOMASUNDARAM, B ; MORTON, A. J ; EMSON, P. C ; MASON, W. T</creatorcontrib><description>Previous work demonstrating the presence and differential distribution of Ca(2+)-binding proteins in the CNS has led to the proposal that cytosolic proteins, such as calbindin-D28k (CB), may play a pivotal role in neurons. We have used a retrovirus containing the full-length cDNA for CB to transfect the pituitary tumor cell line GH3, to generate CB-expressing GH3 cells and to investigate whether ionic channel activities as well as the concentration of intracellular free Ca2+ ([Ca2+]i) homeostasis could be altered by the presence of this Ca(2+)-binding protein. We show that CB-transfected GH3 cells exhibited lower Ca2+ entry through voltage-dependent Ca2+ channels and were better able to reduce [Ca2+]i transients evoked by voltage depolarizations than the wild-type parent cell line. These observations provide a mechanism by which CB may protect tissues against Ca(2+)-mediated excitotoxicity.</description><identifier>ISSN: 0896-6273</identifier><identifier>EISSN: 1097-4199</identifier><identifier>DOI: 10.1016/0896-6273(92)90246-A</identifier><identifier>PMID: 1329864</identifier><identifier>CODEN: NERNET</identifier><language>eng</language><publisher>Cambridge, MA: Cell Press</publisher><subject>Animals ; Biological and medical sciences ; Calbindin 1 ; Calbindins ; Calcium - metabolism ; Calcium Channels - physiology ; Cell Membrane - physiology ; Cell physiology ; Chelating Agents - pharmacology ; Electric Conductivity ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; Genetic Vectors ; Homeostasis - physiology ; Membrane and intracellular transports ; Molecular and cellular biology ; Pituitary Neoplasms ; Rats ; Retroviridae - genetics ; S100 Calcium Binding Protein G - genetics ; S100 Calcium Binding Protein G - physiology ; Transfection ; Tumor Cells, Cultured</subject><ispartof>Neuron (Cambridge, Mass.), 1992-11, Vol.9 (5), p.943-954</ispartof><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c331t-7c81a2d3061c359138afbc746fd17fb8907bff2c75446b3f80456945fe3aed713</citedby><cites>FETCH-LOGICAL-c331t-7c81a2d3061c359138afbc746fd17fb8907bff2c75446b3f80456945fe3aed713</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4458054$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1329864$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>LLEDO, P.-M</creatorcontrib><creatorcontrib>SOMASUNDARAM, B</creatorcontrib><creatorcontrib>MORTON, A. J</creatorcontrib><creatorcontrib>EMSON, P. C</creatorcontrib><creatorcontrib>MASON, W. T</creatorcontrib><title>Stable transfection of calbindin-D28k into the GH3 cell line alters calcium currents and intracellular calcium homeostasis</title><title>Neuron (Cambridge, Mass.)</title><addtitle>Neuron</addtitle><description>Previous work demonstrating the presence and differential distribution of Ca(2+)-binding proteins in the CNS has led to the proposal that cytosolic proteins, such as calbindin-D28k (CB), may play a pivotal role in neurons. We have used a retrovirus containing the full-length cDNA for CB to transfect the pituitary tumor cell line GH3, to generate CB-expressing GH3 cells and to investigate whether ionic channel activities as well as the concentration of intracellular free Ca2+ ([Ca2+]i) homeostasis could be altered by the presence of this Ca(2+)-binding protein. We show that CB-transfected GH3 cells exhibited lower Ca2+ entry through voltage-dependent Ca2+ channels and were better able to reduce [Ca2+]i transients evoked by voltage depolarizations than the wild-type parent cell line. These observations provide a mechanism by which CB may protect tissues against Ca(2+)-mediated excitotoxicity.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Calbindin 1</subject><subject>Calbindins</subject><subject>Calcium - metabolism</subject><subject>Calcium Channels - physiology</subject><subject>Cell Membrane - physiology</subject><subject>Cell physiology</subject><subject>Chelating Agents - pharmacology</subject><subject>Electric Conductivity</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>Genetic Vectors</subject><subject>Homeostasis - physiology</subject><subject>Membrane and intracellular transports</subject><subject>Molecular and cellular biology</subject><subject>Pituitary Neoplasms</subject><subject>Rats</subject><subject>Retroviridae - genetics</subject><subject>S100 Calcium Binding Protein G - genetics</subject><subject>S100 Calcium Binding Protein G - physiology</subject><subject>Transfection</subject><subject>Tumor Cells, Cultured</subject><issn>0896-6273</issn><issn>1097-4199</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkEtPxCAUhYnRjOPjH2jCwhhdVKFQHsvJ-BgTExfqmlAKEW2pAl3or7d1JuPqLs53Tm4-AE4wusIIs2skJCtYycmFLC8lKikrFjtgjpHkBcVS7oL5FtkHBym9I4RpJfEMzDAppWB0Dn6es65bC3PUITlrsu8D7B00uq19aHwobkrxAX3IPcxvFt6vCDS2bWHrg4W6zTamCTZ-6KAZYrQhJ6hDM1WintCh1XGLvPWd7VPWyacjsOd0m-zx5h6C17vbl-WqeHy6f1guHgtDCM4FNwLrsiGIYUPG74nQrjacMtdg7mohEa-dKw2vKGU1cQLRiklaOUu0bTgmh-B8vfsZ-6_Bpqw6n6bHdLD9kBQn4zYXZATpGjSxTylapz6j73T8VhipSbmafKrJp5Kl-lOuFmPtdLM_1J1t_ktrx2N-tsl1GjW40bTxaYtRWglUUfIL3lCKRg</recordid><startdate>19921101</startdate><enddate>19921101</enddate><creator>LLEDO, P.-M</creator><creator>SOMASUNDARAM, B</creator><creator>MORTON, A. J</creator><creator>EMSON, P. C</creator><creator>MASON, W. T</creator><general>Cell Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19921101</creationdate><title>Stable transfection of calbindin-D28k into the GH3 cell line alters calcium currents and intracellular calcium homeostasis</title><author>LLEDO, P.-M ; SOMASUNDARAM, B ; MORTON, A. J ; EMSON, P. C ; MASON, W. T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c331t-7c81a2d3061c359138afbc746fd17fb8907bff2c75446b3f80456945fe3aed713</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Calbindin 1</topic><topic>Calbindins</topic><topic>Calcium - metabolism</topic><topic>Calcium Channels - physiology</topic><topic>Cell Membrane - physiology</topic><topic>Cell physiology</topic><topic>Chelating Agents - pharmacology</topic><topic>Electric Conductivity</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>Genetic Vectors</topic><topic>Homeostasis - physiology</topic><topic>Membrane and intracellular transports</topic><topic>Molecular and cellular biology</topic><topic>Pituitary Neoplasms</topic><topic>Rats</topic><topic>Retroviridae - genetics</topic><topic>S100 Calcium Binding Protein G - genetics</topic><topic>S100 Calcium Binding Protein G - physiology</topic><topic>Transfection</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>LLEDO, P.-M</creatorcontrib><creatorcontrib>SOMASUNDARAM, B</creatorcontrib><creatorcontrib>MORTON, A. J</creatorcontrib><creatorcontrib>EMSON, P. C</creatorcontrib><creatorcontrib>MASON, W. T</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Neuron (Cambridge, Mass.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>LLEDO, P.-M</au><au>SOMASUNDARAM, B</au><au>MORTON, A. J</au><au>EMSON, P. C</au><au>MASON, W. T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Stable transfection of calbindin-D28k into the GH3 cell line alters calcium currents and intracellular calcium homeostasis</atitle><jtitle>Neuron (Cambridge, Mass.)</jtitle><addtitle>Neuron</addtitle><date>1992-11-01</date><risdate>1992</risdate><volume>9</volume><issue>5</issue><spage>943</spage><epage>954</epage><pages>943-954</pages><issn>0896-6273</issn><eissn>1097-4199</eissn><coden>NERNET</coden><abstract>Previous work demonstrating the presence and differential distribution of Ca(2+)-binding proteins in the CNS has led to the proposal that cytosolic proteins, such as calbindin-D28k (CB), may play a pivotal role in neurons. We have used a retrovirus containing the full-length cDNA for CB to transfect the pituitary tumor cell line GH3, to generate CB-expressing GH3 cells and to investigate whether ionic channel activities as well as the concentration of intracellular free Ca2+ ([Ca2+]i) homeostasis could be altered by the presence of this Ca(2+)-binding protein. We show that CB-transfected GH3 cells exhibited lower Ca2+ entry through voltage-dependent Ca2+ channels and were better able to reduce [Ca2+]i transients evoked by voltage depolarizations than the wild-type parent cell line. These observations provide a mechanism by which CB may protect tissues against Ca(2+)-mediated excitotoxicity.</abstract><cop>Cambridge, MA</cop><pub>Cell Press</pub><pmid>1329864</pmid><doi>10.1016/0896-6273(92)90246-A</doi><tpages>12</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0896-6273 |
| ispartof | Neuron (Cambridge, Mass.), 1992-11, Vol.9 (5), p.943-954 |
| issn | 0896-6273 1097-4199 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73306783 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Biological and medical sciences Calbindin 1 Calbindins Calcium - metabolism Calcium Channels - physiology Cell Membrane - physiology Cell physiology Chelating Agents - pharmacology Electric Conductivity Fundamental and applied biological sciences. Psychology Gene Expression Genetic Vectors Homeostasis - physiology Membrane and intracellular transports Molecular and cellular biology Pituitary Neoplasms Rats Retroviridae - genetics S100 Calcium Binding Protein G - genetics S100 Calcium Binding Protein G - physiology Transfection Tumor Cells, Cultured |
| title | Stable transfection of calbindin-D28k into the GH3 cell line alters calcium currents and intracellular calcium homeostasis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-25T07%3A48%3A07IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Stable%20transfection%20of%20calbindin-D28k%20into%20the%20GH3%20cell%20line%20alters%20calcium%20currents%20and%20intracellular%20calcium%20homeostasis&rft.jtitle=Neuron%20(Cambridge,%20Mass.)&rft.au=LLEDO,%20P.-M&rft.date=1992-11-01&rft.volume=9&rft.issue=5&rft.spage=943&rft.epage=954&rft.pages=943-954&rft.issn=0896-6273&rft.eissn=1097-4199&rft.coden=NERNET&rft_id=info:doi/10.1016/0896-6273(92)90246-A&rft_dat=%3Cproquest_cross%3E73306783%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=73306783&rft_id=info:pmid/1329864&rfr_iscdi=true |