Human Sertoli cells in vitro : lactate, estradiol-17β and transferrin production
Human Sertoli cell parameters, namely lactate, estradiol-17 beta, and transferrin production, were determined after a 24-hour incubation with either human follicle stimulating hormone (FSH) or dbcAMP in the presence or absence of testosterone plus a phosphodiesterase inhibitor (1-methyl-3-isobutylxa...
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| Veröffentlicht in: | Journal of andrology 1992-09, Vol.13 (5), p.361-367 |
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| container_title | Journal of andrology |
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| creator | FOUCAULT, P CARREAU, S KUCZYNSKI, W GUILLAUMIN, J. M BARDOS, P DROSDOWSKY, M. A |
| description | Human Sertoli cell parameters, namely lactate, estradiol-17 beta, and transferrin production, were determined after a 24-hour incubation with either human follicle stimulating hormone (FSH) or dbcAMP in the presence or absence of testosterone plus a phosphodiesterase inhibitor (1-methyl-3-isobutylxanthine; MIX). Testicular tissues were obtained from 10 young patients (mean age, 29 years); using a 3-step enzymatic treatment, Sertoli cell enriched preparations (> 92%) were studied after 4 days as primary cultures. No significant changes in lactate, estradiol-17 beta, and transferrin outputs have been observed according to age in patients ranging in age from 16 years to 47 years. Sertoli cell production of the compounds is controlled by testosterone plus MIX; FSH (or dbcAMP) treatment only slightly improves their synthesis. It is suggested that human Sertoli cell function, as far as the parameters measured in this study are concerned, is likely regulated by cAMP-dependent and independent pathways. |
| doi_str_mv | 10.1002/j.1939-4640.1992.tb03325.x |
| format | Article |
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M ; BARDOS, P ; DROSDOWSKY, M. A</creator><creatorcontrib>FOUCAULT, P ; CARREAU, S ; KUCZYNSKI, W ; GUILLAUMIN, J. M ; BARDOS, P ; DROSDOWSKY, M. A</creatorcontrib><description>Human Sertoli cell parameters, namely lactate, estradiol-17 beta, and transferrin production, were determined after a 24-hour incubation with either human follicle stimulating hormone (FSH) or dbcAMP in the presence or absence of testosterone plus a phosphodiesterase inhibitor (1-methyl-3-isobutylxanthine; MIX). Testicular tissues were obtained from 10 young patients (mean age, 29 years); using a 3-step enzymatic treatment, Sertoli cell enriched preparations (> 92%) were studied after 4 days as primary cultures. No significant changes in lactate, estradiol-17 beta, and transferrin outputs have been observed according to age in patients ranging in age from 16 years to 47 years. Sertoli cell production of the compounds is controlled by testosterone plus MIX; FSH (or dbcAMP) treatment only slightly improves their synthesis. It is suggested that human Sertoli cell function, as far as the parameters measured in this study are concerned, is likely regulated by cAMP-dependent and independent pathways.</description><identifier>ISSN: 0196-3635</identifier><identifier>EISSN: 1939-4640</identifier><identifier>DOI: 10.1002/j.1939-4640.1992.tb03325.x</identifier><identifier>PMID: 1429217</identifier><identifier>CODEN: JOAND3</identifier><language>eng</language><publisher>San Fransisco, CA: American Society of Andrology</publisher><subject>Adolescent ; Adult ; Biological and medical sciences ; Cells, Cultured ; Estradiol - biosynthesis ; Follicle Stimulating Hormone - blood ; Follicle Stimulating Hormone - physiology ; Fundamental and applied biological sciences. Psychology ; Humans ; Lactates - biosynthesis ; Luteinizing Hormone - blood ; Luteinizing Hormone - physiology ; Male ; Mammalian male genital system ; Middle Aged ; Morphology. Physiology ; Sertoli Cells - metabolism ; Testosterone - blood ; Testosterone - physiology ; Transferrin - biosynthesis ; Vertebrates: reproduction</subject><ispartof>Journal of andrology, 1992-09, Vol.13 (5), p.361-367</ispartof><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c201t-d30732d480729908fcf53a52e2d8756cc88c6fe1328a40ff5deffdc85c8895783</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4321089$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1429217$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>FOUCAULT, P</creatorcontrib><creatorcontrib>CARREAU, S</creatorcontrib><creatorcontrib>KUCZYNSKI, W</creatorcontrib><creatorcontrib>GUILLAUMIN, J. M</creatorcontrib><creatorcontrib>BARDOS, P</creatorcontrib><creatorcontrib>DROSDOWSKY, M. A</creatorcontrib><title>Human Sertoli cells in vitro : lactate, estradiol-17β and transferrin production</title><title>Journal of andrology</title><addtitle>J Androl</addtitle><description>Human Sertoli cell parameters, namely lactate, estradiol-17 beta, and transferrin production, were determined after a 24-hour incubation with either human follicle stimulating hormone (FSH) or dbcAMP in the presence or absence of testosterone plus a phosphodiesterase inhibitor (1-methyl-3-isobutylxanthine; MIX). Testicular tissues were obtained from 10 young patients (mean age, 29 years); using a 3-step enzymatic treatment, Sertoli cell enriched preparations (> 92%) were studied after 4 days as primary cultures. No significant changes in lactate, estradiol-17 beta, and transferrin outputs have been observed according to age in patients ranging in age from 16 years to 47 years. Sertoli cell production of the compounds is controlled by testosterone plus MIX; FSH (or dbcAMP) treatment only slightly improves their synthesis. It is suggested that human Sertoli cell function, as far as the parameters measured in this study are concerned, is likely regulated by cAMP-dependent and independent pathways.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Biological and medical sciences</subject><subject>Cells, Cultured</subject><subject>Estradiol - biosynthesis</subject><subject>Follicle Stimulating Hormone - blood</subject><subject>Follicle Stimulating Hormone - physiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Lactates - biosynthesis</subject><subject>Luteinizing Hormone - blood</subject><subject>Luteinizing Hormone - physiology</subject><subject>Male</subject><subject>Mammalian male genital system</subject><subject>Middle Aged</subject><subject>Morphology. Physiology</subject><subject>Sertoli Cells - metabolism</subject><subject>Testosterone - blood</subject><subject>Testosterone - physiology</subject><subject>Transferrin - biosynthesis</subject><subject>Vertebrates: reproduction</subject><issn>0196-3635</issn><issn>1939-4640</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkNtKAzEURYMotVY_QQgiPjljLjOTpG9S1AoFEfU5pLnAlLnUZEbqb_khfpMZOtSnA2fvfc5mAXCFUYoRInebFAsqkqzI4kIIknZrRCnJ090RmB6kYzBFWBQJLWh-Cs5C2MQswoxOwARnRBDMpuB12deqgW_Wd21VQm2rKsCygV9l51s4h5XSnersLbSh88qUbZVg9vsDVWNgXDTBWe-jf-tb0-uubJtzcOJUFezFOGfg4_HhfbFMVi9Pz4v7VaJjiS4xFDFKTMYRI0Ig7rTLqcqJJYazvNCac104iynhKkPO5cY6ZzTPoyByxukM3Ozvxteffawn6zIM_VVj2z5IRinClOFonO-N2rcheOvk1pe18t8SIznwlBs5QJMDNDnwlCNPuYvhy_FLv66t-Y_uAUb9etRV0KpyEYkuw8GWUYIRF_QPY-h_qg</recordid><startdate>199209</startdate><enddate>199209</enddate><creator>FOUCAULT, P</creator><creator>CARREAU, S</creator><creator>KUCZYNSKI, W</creator><creator>GUILLAUMIN, J. M</creator><creator>BARDOS, P</creator><creator>DROSDOWSKY, M. A</creator><general>American Society of Andrology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199209</creationdate><title>Human Sertoli cells in vitro : lactate, estradiol-17β and transferrin production</title><author>FOUCAULT, P ; CARREAU, S ; KUCZYNSKI, W ; GUILLAUMIN, J. M ; BARDOS, P ; DROSDOWSKY, M. A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c201t-d30732d480729908fcf53a52e2d8756cc88c6fe1328a40ff5deffdc85c8895783</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Biological and medical sciences</topic><topic>Cells, Cultured</topic><topic>Estradiol - biosynthesis</topic><topic>Follicle Stimulating Hormone - blood</topic><topic>Follicle Stimulating Hormone - physiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Lactates - biosynthesis</topic><topic>Luteinizing Hormone - blood</topic><topic>Luteinizing Hormone - physiology</topic><topic>Male</topic><topic>Mammalian male genital system</topic><topic>Middle Aged</topic><topic>Morphology. Physiology</topic><topic>Sertoli Cells - metabolism</topic><topic>Testosterone - blood</topic><topic>Testosterone - physiology</topic><topic>Transferrin - biosynthesis</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>FOUCAULT, P</creatorcontrib><creatorcontrib>CARREAU, S</creatorcontrib><creatorcontrib>KUCZYNSKI, W</creatorcontrib><creatorcontrib>GUILLAUMIN, J. M</creatorcontrib><creatorcontrib>BARDOS, P</creatorcontrib><creatorcontrib>DROSDOWSKY, M. 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A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human Sertoli cells in vitro : lactate, estradiol-17β and transferrin production</atitle><jtitle>Journal of andrology</jtitle><addtitle>J Androl</addtitle><date>1992-09</date><risdate>1992</risdate><volume>13</volume><issue>5</issue><spage>361</spage><epage>367</epage><pages>361-367</pages><issn>0196-3635</issn><eissn>1939-4640</eissn><coden>JOAND3</coden><abstract>Human Sertoli cell parameters, namely lactate, estradiol-17 beta, and transferrin production, were determined after a 24-hour incubation with either human follicle stimulating hormone (FSH) or dbcAMP in the presence or absence of testosterone plus a phosphodiesterase inhibitor (1-methyl-3-isobutylxanthine; MIX). Testicular tissues were obtained from 10 young patients (mean age, 29 years); using a 3-step enzymatic treatment, Sertoli cell enriched preparations (> 92%) were studied after 4 days as primary cultures. No significant changes in lactate, estradiol-17 beta, and transferrin outputs have been observed according to age in patients ranging in age from 16 years to 47 years. Sertoli cell production of the compounds is controlled by testosterone plus MIX; FSH (or dbcAMP) treatment only slightly improves their synthesis. It is suggested that human Sertoli cell function, as far as the parameters measured in this study are concerned, is likely regulated by cAMP-dependent and independent pathways.</abstract><cop>San Fransisco, CA</cop><pub>American Society of Andrology</pub><pmid>1429217</pmid><doi>10.1002/j.1939-4640.1992.tb03325.x</doi><tpages>7</tpages></addata></record> |
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| identifier | ISSN: 0196-3635 |
| ispartof | Journal of andrology, 1992-09, Vol.13 (5), p.361-367 |
| issn | 0196-3635 1939-4640 |
| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Adolescent Adult Biological and medical sciences Cells, Cultured Estradiol - biosynthesis Follicle Stimulating Hormone - blood Follicle Stimulating Hormone - physiology Fundamental and applied biological sciences. Psychology Humans Lactates - biosynthesis Luteinizing Hormone - blood Luteinizing Hormone - physiology Male Mammalian male genital system Middle Aged Morphology. Physiology Sertoli Cells - metabolism Testosterone - blood Testosterone - physiology Transferrin - biosynthesis Vertebrates: reproduction |
| title | Human Sertoli cells in vitro : lactate, estradiol-17β and transferrin production |
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