Identification of infant and adult swine susceptible to enterotoxigenic Escherichia coli by detection of receptors for F4(K88)ac fimbriae in brush borders or feces
We attempted to determine F4(K88)-adhesive and non-adhesive phenotypes of infant (neonatal
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| Veröffentlicht in: | Comparative immunology, microbiology and infectious diseases microbiology and infectious diseases, 1992-10, Vol.15 (4), p.271-279 |
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| creator | Valpotic, I Frankovic, M Vrbanac, I |
| description | We attempted to determine F4(K88)-adhesive and non-adhesive phenotypes of infant (neonatal |
| doi_str_mv | 10.1016/0147-9571(92)90006-D |
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| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_73296616</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>014795719290006D</els_id><sourcerecordid>16640211</sourcerecordid><originalsourceid>FETCH-LOGICAL-c417t-4e62d494d0e0fdaeff7a055c26be06f9a2276fb0a4e770da762c4394da1a864a3</originalsourceid><addsrcrecordid>eNqFkc2OFCEURonRjO3oG2jCwpiZRSlUUVC1MTHz58RJ3Oia3IKLjamGFih1nscXlbZ7xp2uWNzzfcA9hDzn7DVnXL5hXKhm7BU_GdvTkTEmm_MHZMUHNTRcSvWQrO6Rx-RJzl8rM3LBj8gR7_qhZ3xFfl1bDMU7b6D4GGh01AcHoVAIloJd5kLzDx-Q5iUb3BY_zUhLpDWFKZb403_B4A29yGaNyZu1B2ri7Ol0Sy0WNHe1CXfxmDJ1MdFLcfJhGE7BUOc3U_KA9V46pSWv6RSTxcpVzNVQfkoeOZgzPjucx-Tz5cWns_fNzcer67N3N40RXJVGoGytGIVlyJwFdE4B63vTygmZdCO0rZJuYiBQKWZBydaIrvLAYZACumPyat-7TfHbgrnoja9_nmcIGJesVdeOUnL5X7BuX7CW8wqKPWhSzDmh09vkN5BuNWd6J1HvDOmdIT22-o9EfV5jLw79y7RB-ze0t1bnLw9zyAZmlyAYn-8xIVQnmKrY2z2GdWnfPSadjcdg0Poqo2gb_b_f8Ruwtbrc</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16640211</pqid></control><display><type>article</type><title>Identification of infant and adult swine susceptible to enterotoxigenic Escherichia coli by detection of receptors for F4(K88)ac fimbriae in brush borders or feces</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Valpotic, I ; Frankovic, M ; Vrbanac, I</creator><creatorcontrib>Valpotic, I ; Frankovic, M ; Vrbanac, I</creatorcontrib><description><![CDATA[We attempted to determine F4(K88)-adhesive and non-adhesive phenotypes of infant (neonatal <3 day old and weaned <4 week old pigs) and adult (>6 month old) swine by ELISA using immobilized F4(K88)ac fimbrial antigen or whole F4(K88) +
E. coli cells (strains M1823 and 1476) and isolated small intestinal brush borders or easily-obtainable fecal samples from the same animals. Nineteen of 22 neonates (86%), 17 of 20 weaners (85%), and 26 of 39 adults (67%) were classified identically as F4(K88) receptor-positive or negative by the ELISA. The ELISA with feces from adult swine was found to be almost equally specific (87%) as that with feces from neonatal (90%) and weaned (91%) pigs. However, the sensitivity of the assay was low (38%), indicating that fecal samples from adults contained less receptor-material than necessary for comparable phenotyping. The receptor-positive brush borders from neonates and weaners reacted significantly better (
P < 0.02, < 0.001 respectively) with purified F4(K88) antigen than did those from adults. There was good agreement between the average ELISA values for feces from infant and adult swine regardless the source of coating antigen applied. With this assay we can determine F4(K88) phenotypes of infant swine using easily-collected fecal samples rather than isolated brush borders. It was also concluded that tested feces is not an acceptable alternate source of the receptor-material to brush borders from F4(K88)-susceptible adult swine.
Nous avons essayé de déterminer des phénotypes adhésifs et non adhésifs de F4(K88) chez le porcelet (nouveau né de moins de 3 jours et sevrés de moins de 4 semaines) et le porc adulte (de plus de 6 mois) par la méthode ELISA utilisant l'antigène fimbrial F4(K88) immobilisé ou l'ensemble F4(K88) +
E. coli (souches M 1823 et 1476), et nous avons isolé des fragments de bordures en brosse intestinales ou des échantillons de fèces des mêmes animaux. 19 des 22 nouveaux-nés (86%), 17 des 20 porcs sevrés (85%) et 26 des 39 porcs adultes (67%) ont été considérés identiques quant à leurs récepteurs à F4 avec la méthode ELISA. La méthode ELISA utilisée est sensiblement aussi spécifique sur des fèces de porc adulte (87%) que sur des fèces de nouveaux-nés (90%) ou des porcs sevrés (91%). Cependant, la sensibilité de cet essai a été basse (38%), ce qui indique que les échantillons fécaux des adultes ne contenaient pas assez de récepteurs pour une étude comparable des phénotypes. Le récepteur des bordures en brosse des nouveaux-nés et des sevrés a significativement mieux réagi (
P < 0.02 et < 0.001 respectivement) avec l'antigène purifié F4(K88) que ne le firent ceux des adultes. II y a eu une bonne corrélation entre les valeurs moyennes de la méthode ELISA pour les fèces des porcelets et des porcs adultes si l'on ne tient pas compte du manteau d'antigène appliqué. Avec cet essai, nous pouvons déterminer les phénotypes F4(K88) du porcelet en utilisant des échantillons de fèces, plus facilement collectés que des bordures en brosse isolées. Nous avons aussi conclu que les fèces testés ne sont pas une source alternative acceptable de récepteurs des bordures en brosse de porcs adultes réceptifs à F4(K88).]]></description><identifier>ISSN: 0147-9571</identifier><identifier>EISSN: 1878-1667</identifier><identifier>DOI: 10.1016/0147-9571(92)90006-D</identifier><identifier>PMID: 1358501</identifier><identifier>CODEN: CIMIDV</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Adhesins, Escherichia coli ; Animals ; Animals, Newborn ; Bacterial Outer Membrane Proteins - metabolism ; Bacteriology ; Biological and medical sciences ; Disease Susceptibility ; E. coli ; ELISA detection ; ELISA détection ; Enzyme-Linked Immunosorbent Assay ; Escherichia coli ; Escherichia coli Infections - immunology ; feces ; Feces - microbiology ; Fimbriae, Bacterial - metabolism ; Fundamental and applied biological sciences. Psychology ; fèces ; Intestinal F4(K88) receptor ; Intestinal F4(K88) récepteur ; Microbiology ; Microvilli - microbiology ; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains ; porc ; Receptors, Immunologic - analysis ; Receptors, Immunologic - metabolism ; swine ; Swine - immunology ; Swine - microbiology</subject><ispartof>Comparative immunology, microbiology and infectious diseases, 1992-10, Vol.15 (4), p.271-279</ispartof><rights>1992</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-4e62d494d0e0fdaeff7a055c26be06f9a2276fb0a4e770da762c4394da1a864a3</citedby><cites>FETCH-LOGICAL-c417t-4e62d494d0e0fdaeff7a055c26be06f9a2276fb0a4e770da762c4394da1a864a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0147-9571(92)90006-D$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4473407$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1358501$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Valpotic, I</creatorcontrib><creatorcontrib>Frankovic, M</creatorcontrib><creatorcontrib>Vrbanac, I</creatorcontrib><title>Identification of infant and adult swine susceptible to enterotoxigenic Escherichia coli by detection of receptors for F4(K88)ac fimbriae in brush borders or feces</title><title>Comparative immunology, microbiology and infectious diseases</title><addtitle>Comp Immunol Microbiol Infect Dis</addtitle><description><![CDATA[We attempted to determine F4(K88)-adhesive and non-adhesive phenotypes of infant (neonatal <3 day old and weaned <4 week old pigs) and adult (>6 month old) swine by ELISA using immobilized F4(K88)ac fimbrial antigen or whole F4(K88) +
E. coli cells (strains M1823 and 1476) and isolated small intestinal brush borders or easily-obtainable fecal samples from the same animals. Nineteen of 22 neonates (86%), 17 of 20 weaners (85%), and 26 of 39 adults (67%) were classified identically as F4(K88) receptor-positive or negative by the ELISA. The ELISA with feces from adult swine was found to be almost equally specific (87%) as that with feces from neonatal (90%) and weaned (91%) pigs. However, the sensitivity of the assay was low (38%), indicating that fecal samples from adults contained less receptor-material than necessary for comparable phenotyping. The receptor-positive brush borders from neonates and weaners reacted significantly better (
P < 0.02, < 0.001 respectively) with purified F4(K88) antigen than did those from adults. There was good agreement between the average ELISA values for feces from infant and adult swine regardless the source of coating antigen applied. With this assay we can determine F4(K88) phenotypes of infant swine using easily-collected fecal samples rather than isolated brush borders. It was also concluded that tested feces is not an acceptable alternate source of the receptor-material to brush borders from F4(K88)-susceptible adult swine.
Nous avons essayé de déterminer des phénotypes adhésifs et non adhésifs de F4(K88) chez le porcelet (nouveau né de moins de 3 jours et sevrés de moins de 4 semaines) et le porc adulte (de plus de 6 mois) par la méthode ELISA utilisant l'antigène fimbrial F4(K88) immobilisé ou l'ensemble F4(K88) +
E. coli (souches M 1823 et 1476), et nous avons isolé des fragments de bordures en brosse intestinales ou des échantillons de fèces des mêmes animaux. 19 des 22 nouveaux-nés (86%), 17 des 20 porcs sevrés (85%) et 26 des 39 porcs adultes (67%) ont été considérés identiques quant à leurs récepteurs à F4 avec la méthode ELISA. La méthode ELISA utilisée est sensiblement aussi spécifique sur des fèces de porc adulte (87%) que sur des fèces de nouveaux-nés (90%) ou des porcs sevrés (91%). Cependant, la sensibilité de cet essai a été basse (38%), ce qui indique que les échantillons fécaux des adultes ne contenaient pas assez de récepteurs pour une étude comparable des phénotypes. Le récepteur des bordures en brosse des nouveaux-nés et des sevrés a significativement mieux réagi (
P < 0.02 et < 0.001 respectivement) avec l'antigène purifié F4(K88) que ne le firent ceux des adultes. II y a eu une bonne corrélation entre les valeurs moyennes de la méthode ELISA pour les fèces des porcelets et des porcs adultes si l'on ne tient pas compte du manteau d'antigène appliqué. Avec cet essai, nous pouvons déterminer les phénotypes F4(K88) du porcelet en utilisant des échantillons de fèces, plus facilement collectés que des bordures en brosse isolées. Nous avons aussi conclu que les fèces testés ne sont pas une source alternative acceptable de récepteurs des bordures en brosse de porcs adultes réceptifs à F4(K88).]]></description><subject>Adhesins, Escherichia coli</subject><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Bacterial Outer Membrane Proteins - metabolism</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Disease Susceptibility</subject><subject>E. coli</subject><subject>ELISA detection</subject><subject>ELISA détection</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Escherichia coli</subject><subject>Escherichia coli Infections - immunology</subject><subject>feces</subject><subject>Feces - microbiology</subject><subject>Fimbriae, Bacterial - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>fèces</subject><subject>Intestinal F4(K88) receptor</subject><subject>Intestinal F4(K88) récepteur</subject><subject>Microbiology</subject><subject>Microvilli - microbiology</subject><subject>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</subject><subject>porc</subject><subject>Receptors, Immunologic - analysis</subject><subject>Receptors, Immunologic - metabolism</subject><subject>swine</subject><subject>Swine - immunology</subject><subject>Swine - microbiology</subject><issn>0147-9571</issn><issn>1878-1667</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc2OFCEURonRjO3oG2jCwpiZRSlUUVC1MTHz58RJ3Oia3IKLjamGFih1nscXlbZ7xp2uWNzzfcA9hDzn7DVnXL5hXKhm7BU_GdvTkTEmm_MHZMUHNTRcSvWQrO6Rx-RJzl8rM3LBj8gR7_qhZ3xFfl1bDMU7b6D4GGh01AcHoVAIloJd5kLzDx-Q5iUb3BY_zUhLpDWFKZb403_B4A29yGaNyZu1B2ri7Ol0Sy0WNHe1CXfxmDJ1MdFLcfJhGE7BUOc3U_KA9V46pSWv6RSTxcpVzNVQfkoeOZgzPjucx-Tz5cWns_fNzcer67N3N40RXJVGoGytGIVlyJwFdE4B63vTygmZdCO0rZJuYiBQKWZBydaIrvLAYZACumPyat-7TfHbgrnoja9_nmcIGJesVdeOUnL5X7BuX7CW8wqKPWhSzDmh09vkN5BuNWd6J1HvDOmdIT22-o9EfV5jLw79y7RB-ze0t1bnLw9zyAZmlyAYn-8xIVQnmKrY2z2GdWnfPSadjcdg0Poqo2gb_b_f8Ruwtbrc</recordid><startdate>19921001</startdate><enddate>19921001</enddate><creator>Valpotic, I</creator><creator>Frankovic, M</creator><creator>Vrbanac, I</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7U7</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>19921001</creationdate><title>Identification of infant and adult swine susceptible to enterotoxigenic Escherichia coli by detection of receptors for F4(K88)ac fimbriae in brush borders or feces</title><author>Valpotic, I ; Frankovic, M ; Vrbanac, I</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-4e62d494d0e0fdaeff7a055c26be06f9a2276fb0a4e770da762c4394da1a864a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Adhesins, Escherichia coli</topic><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Bacterial Outer Membrane Proteins - metabolism</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Disease Susceptibility</topic><topic>E. coli</topic><topic>ELISA detection</topic><topic>ELISA détection</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Escherichia coli</topic><topic>Escherichia coli Infections - immunology</topic><topic>feces</topic><topic>Feces - microbiology</topic><topic>Fimbriae, Bacterial - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>fèces</topic><topic>Intestinal F4(K88) receptor</topic><topic>Intestinal F4(K88) récepteur</topic><topic>Microbiology</topic><topic>Microvilli - microbiology</topic><topic>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</topic><topic>porc</topic><topic>Receptors, Immunologic - analysis</topic><topic>Receptors, Immunologic - metabolism</topic><topic>swine</topic><topic>Swine - immunology</topic><topic>Swine - microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Valpotic, I</creatorcontrib><creatorcontrib>Frankovic, M</creatorcontrib><creatorcontrib>Vrbanac, I</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Comparative immunology, microbiology and infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Valpotic, I</au><au>Frankovic, M</au><au>Vrbanac, I</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of infant and adult swine susceptible to enterotoxigenic Escherichia coli by detection of receptors for F4(K88)ac fimbriae in brush borders or feces</atitle><jtitle>Comparative immunology, microbiology and infectious diseases</jtitle><addtitle>Comp Immunol Microbiol Infect Dis</addtitle><date>1992-10-01</date><risdate>1992</risdate><volume>15</volume><issue>4</issue><spage>271</spage><epage>279</epage><pages>271-279</pages><issn>0147-9571</issn><eissn>1878-1667</eissn><coden>CIMIDV</coden><abstract><![CDATA[We attempted to determine F4(K88)-adhesive and non-adhesive phenotypes of infant (neonatal <3 day old and weaned <4 week old pigs) and adult (>6 month old) swine by ELISA using immobilized F4(K88)ac fimbrial antigen or whole F4(K88) +
E. coli cells (strains M1823 and 1476) and isolated small intestinal brush borders or easily-obtainable fecal samples from the same animals. Nineteen of 22 neonates (86%), 17 of 20 weaners (85%), and 26 of 39 adults (67%) were classified identically as F4(K88) receptor-positive or negative by the ELISA. The ELISA with feces from adult swine was found to be almost equally specific (87%) as that with feces from neonatal (90%) and weaned (91%) pigs. However, the sensitivity of the assay was low (38%), indicating that fecal samples from adults contained less receptor-material than necessary for comparable phenotyping. The receptor-positive brush borders from neonates and weaners reacted significantly better (
P < 0.02, < 0.001 respectively) with purified F4(K88) antigen than did those from adults. There was good agreement between the average ELISA values for feces from infant and adult swine regardless the source of coating antigen applied. With this assay we can determine F4(K88) phenotypes of infant swine using easily-collected fecal samples rather than isolated brush borders. It was also concluded that tested feces is not an acceptable alternate source of the receptor-material to brush borders from F4(K88)-susceptible adult swine.
Nous avons essayé de déterminer des phénotypes adhésifs et non adhésifs de F4(K88) chez le porcelet (nouveau né de moins de 3 jours et sevrés de moins de 4 semaines) et le porc adulte (de plus de 6 mois) par la méthode ELISA utilisant l'antigène fimbrial F4(K88) immobilisé ou l'ensemble F4(K88) +
E. coli (souches M 1823 et 1476), et nous avons isolé des fragments de bordures en brosse intestinales ou des échantillons de fèces des mêmes animaux. 19 des 22 nouveaux-nés (86%), 17 des 20 porcs sevrés (85%) et 26 des 39 porcs adultes (67%) ont été considérés identiques quant à leurs récepteurs à F4 avec la méthode ELISA. La méthode ELISA utilisée est sensiblement aussi spécifique sur des fèces de porc adulte (87%) que sur des fèces de nouveaux-nés (90%) ou des porcs sevrés (91%). Cependant, la sensibilité de cet essai a été basse (38%), ce qui indique que les échantillons fécaux des adultes ne contenaient pas assez de récepteurs pour une étude comparable des phénotypes. Le récepteur des bordures en brosse des nouveaux-nés et des sevrés a significativement mieux réagi (
P < 0.02 et < 0.001 respectivement) avec l'antigène purifié F4(K88) que ne le firent ceux des adultes. II y a eu une bonne corrélation entre les valeurs moyennes de la méthode ELISA pour les fèces des porcelets et des porcs adultes si l'on ne tient pas compte du manteau d'antigène appliqué. Avec cet essai, nous pouvons déterminer les phénotypes F4(K88) du porcelet en utilisant des échantillons de fèces, plus facilement collectés que des bordures en brosse isolées. Nous avons aussi conclu que les fèces testés ne sont pas une source alternative acceptable de récepteurs des bordures en brosse de porcs adultes réceptifs à F4(K88).]]></abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>1358501</pmid><doi>10.1016/0147-9571(92)90006-D</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0147-9571 |
| ispartof | Comparative immunology, microbiology and infectious diseases, 1992-10, Vol.15 (4), p.271-279 |
| issn | 0147-9571 1878-1667 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73296616 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Adhesins, Escherichia coli Animals Animals, Newborn Bacterial Outer Membrane Proteins - metabolism Bacteriology Biological and medical sciences Disease Susceptibility E. coli ELISA detection ELISA détection Enzyme-Linked Immunosorbent Assay Escherichia coli Escherichia coli Infections - immunology feces Feces - microbiology Fimbriae, Bacterial - metabolism Fundamental and applied biological sciences. Psychology fèces Intestinal F4(K88) receptor Intestinal F4(K88) récepteur Microbiology Microvilli - microbiology Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains porc Receptors, Immunologic - analysis Receptors, Immunologic - metabolism swine Swine - immunology Swine - microbiology |
| title | Identification of infant and adult swine susceptible to enterotoxigenic Escherichia coli by detection of receptors for F4(K88)ac fimbriae in brush borders or feces |
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