Different effects of amino acid‐based and glucose‐based dialysate from peritoneal dialysis patients on mesothelial cell ultrastructure and function
Background. Peritoneal dialysis fluid (PDF) containing amino acids has been introduced recently aiming to improve the nutritional status of PD patients. Dextrose‐based PDFs have been implicated in progressive functional and structural deterioration of the peritoneal membrane. Limited data are curren...
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| Veröffentlicht in: | Nephrology, dialysis, transplantation dialysis, transplantation, 2003-06, Vol.18 (6), p.1086-1094 |
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| container_title | Nephrology, dialysis, transplantation |
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| creator | Chan, Tak‐Mao Leung, Jack Kok‐Hung Sun, Yuling Lai, Kar‐Neng Tsang, Ryan Chi‐Wai Yung, Susan |
| description | Background. Peritoneal dialysis fluid (PDF) containing amino acids has been introduced recently aiming to improve the nutritional status of PD patients. Dextrose‐based PDFs have been implicated in progressive functional and structural deterioration of the peritoneal membrane. Limited data are currently available regarding the effect of amino acid‐based PDF on the function and ultrastructure of human peritoneal mesothelial cells (HPMCs), which play a critical role in peritoneal membrane pathophysiology. Methods. We investigated the effects of two commercially available PDFs, which utilized dextrose (1.5% Dianeal) or amino acids (1.1% Nutrineal) as the osmotic agent, obtained from patients after a 4 h dwell, on HPMC proliferation (MTT assay and cell counting) and viability [lactate dehydrogenase (LDH)release], interleukin‐6 (IL‐6) secretion (commercial enzyme‐linked immunosorbent assay) and ultrastructure (scanning and transmission electron microscopy). Results. Exposure of HPMCs to 1.5% Dianeal reduced cell proliferation, total cellular protein synthesis, IL‐6 secretion and cell attachment, but prolonged the cell doubling time on recovery, and increased LDH release (P |
| doi_str_mv | 10.1093/ndt/gfg096 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_73296578</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>73296578</sourcerecordid><originalsourceid>FETCH-LOGICAL-c387t-88793af58fd4cd8a348a2d0ffa42c5fa8c17560c2cefb9a1eb4248bfc6a4ece63</originalsourceid><addsrcrecordid>eNpFkcFu1DAQhi0EokvhwgMgX-CAFGrHSewcUQtbpCJ6AKniYk2c8WJI7K3tSPTGI3Dj_XgSXDZqTzOa_9M_o_kJec7ZG856ceLHfLKzO9Z3D8iGNx2raqHah2RTRF6xlvVH5ElK3xljfS3lY3LEa9koIfoN-XPmrMWIPlMsjcmJBkthdj5QMG78--v3AAlHCn6ku2kxIeHdbHQw3STISG0MM91jdDl4hGlVXKJ7yK6YF1dPZ0whf8OpaNTgNNFlyhFSjovJS8T_K-ziTXbBPyWPLEwJn631mHx5_-7z6Xl18Wn74fTtRWWEkrlSSvYCbKvs2JhRgWgU1COzFpratBaU4bLtmKkN2qEHjkNTN2qwpoMGDXbimLw6-O5juF4wZT27dHsceAxL0lLUfddKVcDXB9DEkFJEq_fRzRBvNGf6NgZdYtCHGAr8YnVdhhnHe3T9ewFergAkA5ON4I1L91zTC8UVL1x14FzK-PNOh_hDd1LIVp9ffdVqe6kuP15t9Zn4B1Wjp78</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>73296578</pqid></control><display><type>article</type><title>Different effects of amino acid‐based and glucose‐based dialysate from peritoneal dialysis patients on mesothelial cell ultrastructure and function</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Oxford University Press Journals All Titles (1996-Current)</source><source>Alma/SFX Local Collection</source><creator>Chan, Tak‐Mao ; Leung, Jack Kok‐Hung ; Sun, Yuling ; Lai, Kar‐Neng ; Tsang, Ryan Chi‐Wai ; Yung, Susan</creator><creatorcontrib>Chan, Tak‐Mao ; Leung, Jack Kok‐Hung ; Sun, Yuling ; Lai, Kar‐Neng ; Tsang, Ryan Chi‐Wai ; Yung, Susan</creatorcontrib><description><![CDATA[Background. Peritoneal dialysis fluid (PDF) containing amino acids has been introduced recently aiming to improve the nutritional status of PD patients. Dextrose‐based PDFs have been implicated in progressive functional and structural deterioration of the peritoneal membrane. Limited data are currently available regarding the effect of amino acid‐based PDF on the function and ultrastructure of human peritoneal mesothelial cells (HPMCs), which play a critical role in peritoneal membrane pathophysiology. Methods. We investigated the effects of two commercially available PDFs, which utilized dextrose (1.5% Dianeal) or amino acids (1.1% Nutrineal) as the osmotic agent, obtained from patients after a 4 h dwell, on HPMC proliferation (MTT assay and cell counting) and viability [lactate dehydrogenase (LDH)release], interleukin‐6 (IL‐6) secretion (commercial enzyme‐linked immunosorbent assay) and ultrastructure (scanning and transmission electron microscopy). Results. Exposure of HPMCs to 1.5% Dianeal reduced cell proliferation, total cellular protein synthesis, IL‐6 secretion and cell attachment, but prolonged the cell doubling time on recovery, and increased LDH release (P<0.001, P<0.001, P<0.0001, P<0.0001, P<0.001 and P<0.001, respectively). The 1.1% Nutrineal reduced HPMC proliferation (P<0.001) and increased IL‐6 secretion (P<0.0001), but did not affect cell attachment, LDH release, protein synthesis or cell doubling time. Ultrastructural studies of HPMCs exposed to Dianeal showed cell flattening, increased cell surface area, reduced microvilli, and intracellular organelles compatible with dysfunctional mitochondria. In contrast, the ultrastructural morphology of HPMCs was relatively preserved after incubation with Nutrineal. Conclusions. Our results showed that HPMC ultrastructure, viability and protein synthesis were better preserved with amino acid‐based PDF, compared with conventional dextrose‐based PDF. The significance of IL‐6 induction by Nutrineal remains to be elucidated.]]></description><identifier>ISSN: 0931-0509</identifier><identifier>EISSN: 1460-2385</identifier><identifier>DOI: 10.1093/ndt/gfg096</identifier><identifier>PMID: 12748339</identifier><identifier>CODEN: NDTREA</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Adult ; amino acid ; Amino Acids - pharmacology ; Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy ; Biological and medical sciences ; Cell Survival ; Cells, Cultured ; Dialysis Solutions - pharmacology ; Emergency and intensive care: renal failure. Dialysis management ; Female ; glucose ; Glucose - pharmacology ; Humans ; Intensive care medicine ; Interleukin-6 - metabolism ; interleukin‐6 ; Kidney Failure, Chronic - therapy ; Male ; Medical sciences ; mesothelial cell ; Middle Aged ; Peritoneal Dialysis ; Peritoneum - cytology ; Peritoneum - drug effects</subject><ispartof>Nephrology, dialysis, transplantation, 2003-06, Vol.18 (6), p.1086-1094</ispartof><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c387t-88793af58fd4cd8a348a2d0ffa42c5fa8c17560c2cefb9a1eb4248bfc6a4ece63</citedby><cites>FETCH-LOGICAL-c387t-88793af58fd4cd8a348a2d0ffa42c5fa8c17560c2cefb9a1eb4248bfc6a4ece63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14938181$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12748339$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chan, Tak‐Mao</creatorcontrib><creatorcontrib>Leung, Jack Kok‐Hung</creatorcontrib><creatorcontrib>Sun, Yuling</creatorcontrib><creatorcontrib>Lai, Kar‐Neng</creatorcontrib><creatorcontrib>Tsang, Ryan Chi‐Wai</creatorcontrib><creatorcontrib>Yung, Susan</creatorcontrib><title>Different effects of amino acid‐based and glucose‐based dialysate from peritoneal dialysis patients on mesothelial cell ultrastructure and function</title><title>Nephrology, dialysis, transplantation</title><addtitle>Nephrol. Dial. Transplant</addtitle><description><![CDATA[Background. Peritoneal dialysis fluid (PDF) containing amino acids has been introduced recently aiming to improve the nutritional status of PD patients. Dextrose‐based PDFs have been implicated in progressive functional and structural deterioration of the peritoneal membrane. Limited data are currently available regarding the effect of amino acid‐based PDF on the function and ultrastructure of human peritoneal mesothelial cells (HPMCs), which play a critical role in peritoneal membrane pathophysiology. Methods. We investigated the effects of two commercially available PDFs, which utilized dextrose (1.5% Dianeal) or amino acids (1.1% Nutrineal) as the osmotic agent, obtained from patients after a 4 h dwell, on HPMC proliferation (MTT assay and cell counting) and viability [lactate dehydrogenase (LDH)release], interleukin‐6 (IL‐6) secretion (commercial enzyme‐linked immunosorbent assay) and ultrastructure (scanning and transmission electron microscopy). Results. Exposure of HPMCs to 1.5% Dianeal reduced cell proliferation, total cellular protein synthesis, IL‐6 secretion and cell attachment, but prolonged the cell doubling time on recovery, and increased LDH release (P<0.001, P<0.001, P<0.0001, P<0.0001, P<0.001 and P<0.001, respectively). The 1.1% Nutrineal reduced HPMC proliferation (P<0.001) and increased IL‐6 secretion (P<0.0001), but did not affect cell attachment, LDH release, protein synthesis or cell doubling time. Ultrastructural studies of HPMCs exposed to Dianeal showed cell flattening, increased cell surface area, reduced microvilli, and intracellular organelles compatible with dysfunctional mitochondria. In contrast, the ultrastructural morphology of HPMCs was relatively preserved after incubation with Nutrineal. Conclusions. Our results showed that HPMC ultrastructure, viability and protein synthesis were better preserved with amino acid‐based PDF, compared with conventional dextrose‐based PDF. The significance of IL‐6 induction by Nutrineal remains to be elucidated.]]></description><subject>Adult</subject><subject>amino acid</subject><subject>Amino Acids - pharmacology</subject><subject>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</subject><subject>Biological and medical sciences</subject><subject>Cell Survival</subject><subject>Cells, Cultured</subject><subject>Dialysis Solutions - pharmacology</subject><subject>Emergency and intensive care: renal failure. Dialysis management</subject><subject>Female</subject><subject>glucose</subject><subject>Glucose - pharmacology</subject><subject>Humans</subject><subject>Intensive care medicine</subject><subject>Interleukin-6 - metabolism</subject><subject>interleukin‐6</subject><subject>Kidney Failure, Chronic - therapy</subject><subject>Male</subject><subject>Medical sciences</subject><subject>mesothelial cell</subject><subject>Middle Aged</subject><subject>Peritoneal Dialysis</subject><subject>Peritoneum - cytology</subject><subject>Peritoneum - drug effects</subject><issn>0931-0509</issn><issn>1460-2385</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkcFu1DAQhi0EokvhwgMgX-CAFGrHSewcUQtbpCJ6AKniYk2c8WJI7K3tSPTGI3Dj_XgSXDZqTzOa_9M_o_kJec7ZG856ceLHfLKzO9Z3D8iGNx2raqHah2RTRF6xlvVH5ElK3xljfS3lY3LEa9koIfoN-XPmrMWIPlMsjcmJBkthdj5QMG78--v3AAlHCn6ku2kxIeHdbHQw3STISG0MM91jdDl4hGlVXKJ7yK6YF1dPZ0whf8OpaNTgNNFlyhFSjovJS8T_K-ziTXbBPyWPLEwJn631mHx5_-7z6Xl18Wn74fTtRWWEkrlSSvYCbKvs2JhRgWgU1COzFpratBaU4bLtmKkN2qEHjkNTN2qwpoMGDXbimLw6-O5juF4wZT27dHsceAxL0lLUfddKVcDXB9DEkFJEq_fRzRBvNGf6NgZdYtCHGAr8YnVdhhnHe3T9ewFergAkA5ON4I1L91zTC8UVL1x14FzK-PNOh_hDd1LIVp9ffdVqe6kuP15t9Zn4B1Wjp78</recordid><startdate>20030601</startdate><enddate>20030601</enddate><creator>Chan, Tak‐Mao</creator><creator>Leung, Jack Kok‐Hung</creator><creator>Sun, Yuling</creator><creator>Lai, Kar‐Neng</creator><creator>Tsang, Ryan Chi‐Wai</creator><creator>Yung, Susan</creator><general>Oxford University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20030601</creationdate><title>Different effects of amino acid‐based and glucose‐based dialysate from peritoneal dialysis patients on mesothelial cell ultrastructure and function</title><author>Chan, Tak‐Mao ; Leung, Jack Kok‐Hung ; Sun, Yuling ; Lai, Kar‐Neng ; Tsang, Ryan Chi‐Wai ; Yung, Susan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c387t-88793af58fd4cd8a348a2d0ffa42c5fa8c17560c2cefb9a1eb4248bfc6a4ece63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Adult</topic><topic>amino acid</topic><topic>Amino Acids - pharmacology</topic><topic>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</topic><topic>Biological and medical sciences</topic><topic>Cell Survival</topic><topic>Cells, Cultured</topic><topic>Dialysis Solutions - pharmacology</topic><topic>Emergency and intensive care: renal failure. Dialysis management</topic><topic>Female</topic><topic>glucose</topic><topic>Glucose - pharmacology</topic><topic>Humans</topic><topic>Intensive care medicine</topic><topic>Interleukin-6 - metabolism</topic><topic>interleukin‐6</topic><topic>Kidney Failure, Chronic - therapy</topic><topic>Male</topic><topic>Medical sciences</topic><topic>mesothelial cell</topic><topic>Middle Aged</topic><topic>Peritoneal Dialysis</topic><topic>Peritoneum - cytology</topic><topic>Peritoneum - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chan, Tak‐Mao</creatorcontrib><creatorcontrib>Leung, Jack Kok‐Hung</creatorcontrib><creatorcontrib>Sun, Yuling</creatorcontrib><creatorcontrib>Lai, Kar‐Neng</creatorcontrib><creatorcontrib>Tsang, Ryan Chi‐Wai</creatorcontrib><creatorcontrib>Yung, Susan</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Nephrology, dialysis, transplantation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chan, Tak‐Mao</au><au>Leung, Jack Kok‐Hung</au><au>Sun, Yuling</au><au>Lai, Kar‐Neng</au><au>Tsang, Ryan Chi‐Wai</au><au>Yung, Susan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Different effects of amino acid‐based and glucose‐based dialysate from peritoneal dialysis patients on mesothelial cell ultrastructure and function</atitle><jtitle>Nephrology, dialysis, transplantation</jtitle><addtitle>Nephrol. Dial. Transplant</addtitle><date>2003-06-01</date><risdate>2003</risdate><volume>18</volume><issue>6</issue><spage>1086</spage><epage>1094</epage><pages>1086-1094</pages><issn>0931-0509</issn><eissn>1460-2385</eissn><coden>NDTREA</coden><abstract><![CDATA[Background. Peritoneal dialysis fluid (PDF) containing amino acids has been introduced recently aiming to improve the nutritional status of PD patients. Dextrose‐based PDFs have been implicated in progressive functional and structural deterioration of the peritoneal membrane. Limited data are currently available regarding the effect of amino acid‐based PDF on the function and ultrastructure of human peritoneal mesothelial cells (HPMCs), which play a critical role in peritoneal membrane pathophysiology. Methods. We investigated the effects of two commercially available PDFs, which utilized dextrose (1.5% Dianeal) or amino acids (1.1% Nutrineal) as the osmotic agent, obtained from patients after a 4 h dwell, on HPMC proliferation (MTT assay and cell counting) and viability [lactate dehydrogenase (LDH)release], interleukin‐6 (IL‐6) secretion (commercial enzyme‐linked immunosorbent assay) and ultrastructure (scanning and transmission electron microscopy). Results. Exposure of HPMCs to 1.5% Dianeal reduced cell proliferation, total cellular protein synthesis, IL‐6 secretion and cell attachment, but prolonged the cell doubling time on recovery, and increased LDH release (P<0.001, P<0.001, P<0.0001, P<0.0001, P<0.001 and P<0.001, respectively). The 1.1% Nutrineal reduced HPMC proliferation (P<0.001) and increased IL‐6 secretion (P<0.0001), but did not affect cell attachment, LDH release, protein synthesis or cell doubling time. Ultrastructural studies of HPMCs exposed to Dianeal showed cell flattening, increased cell surface area, reduced microvilli, and intracellular organelles compatible with dysfunctional mitochondria. In contrast, the ultrastructural morphology of HPMCs was relatively preserved after incubation with Nutrineal. Conclusions. Our results showed that HPMC ultrastructure, viability and protein synthesis were better preserved with amino acid‐based PDF, compared with conventional dextrose‐based PDF. The significance of IL‐6 induction by Nutrineal remains to be elucidated.]]></abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>12748339</pmid><doi>10.1093/ndt/gfg096</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adult amino acid Amino Acids - pharmacology Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy Biological and medical sciences Cell Survival Cells, Cultured Dialysis Solutions - pharmacology Emergency and intensive care: renal failure. Dialysis management Female glucose Glucose - pharmacology Humans Intensive care medicine Interleukin-6 - metabolism interleukin‐6 Kidney Failure, Chronic - therapy Male Medical sciences mesothelial cell Middle Aged Peritoneal Dialysis Peritoneum - cytology Peritoneum - drug effects |
| title | Different effects of amino acid‐based and glucose‐based dialysate from peritoneal dialysis patients on mesothelial cell ultrastructure and function |
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