Increment of Murine Spermatogonial Cell Number by Gonadotropin-Releasing Hormone Analogue Is Independent of Stem Cell Factor c-kit Signal
Recent studies have demonstrated that GnRH-analogues can stimulate regeneration of spermatogenesis of rats when administered after testicular damages. Although the mechanism of this phenomenon has not been elucidated yet, stem cell factor (SCF) produced by Sertoli cells was proposed to mediate the e...
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| Veröffentlicht in: | Biology of reproduction 2003-06, Vol.68 (6), p.2304-2313 |
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| container_title | Biology of reproduction |
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| creator | OHMURA, Masako OGAWA, Takehiko ONO, Michio DEZAWA, Mari HOSAKA, Masahiko KUBOTA, Yoshinobu SAWADA, Hajime |
| description | Recent studies have demonstrated that GnRH-analogues can stimulate regeneration of spermatogenesis of rats when administered
after testicular damages. Although the mechanism of this phenomenon has not been elucidated yet, stem cell factor (SCF) produced
by Sertoli cells was proposed to mediate the effects of GnRH-analogues on spermatogonial proliferation and/or survival. In
the present study, we quantitatively evaluated the proliferation of spermatogonia and addressed whether SCF mediates the effect
of GnRH-analogue on spermatogonial proliferation, using a novel approach combining spermatogonial transplantation and laser
confocal microscopic observation. In the first experiment, using wild-type mice as recipients for spermatogonial transplantation,
the number of donor spermatogonia per 100 Sertoli cells in each spermatogenic colony was significantly higher in the experimental
group of mice treated with leuprorelin, a GnRH-agonist, than that of the control group at 4 and 5 wk after transplantation.
In the second experiment, Steel/Steel dickie (Sl/Sl d ) mutant mice, which lack expression of membrane bound form SCF, were used as recipients. As seen in the first experiment,
the number of undifferentiated spermatogonia was significantly higher in leuprorelin-treated than in the control group. Since
undifferentiated spermatogonia do not express the receptor of SCF, the present study clearly demonstrates that neither membrane-bound
nor secreted forms of SCF are involved in the mechanism of GnRH-analogue's effect on spermatogonial proliferation and/or survival. |
| doi_str_mv | 10.1095/biolreprod.102.013276 |
| format | Article |
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after testicular damages. Although the mechanism of this phenomenon has not been elucidated yet, stem cell factor (SCF) produced
by Sertoli cells was proposed to mediate the effects of GnRH-analogues on spermatogonial proliferation and/or survival. In
the present study, we quantitatively evaluated the proliferation of spermatogonia and addressed whether SCF mediates the effect
of GnRH-analogue on spermatogonial proliferation, using a novel approach combining spermatogonial transplantation and laser
confocal microscopic observation. In the first experiment, using wild-type mice as recipients for spermatogonial transplantation,
the number of donor spermatogonia per 100 Sertoli cells in each spermatogenic colony was significantly higher in the experimental
group of mice treated with leuprorelin, a GnRH-agonist, than that of the control group at 4 and 5 wk after transplantation.
In the second experiment, Steel/Steel dickie (Sl/Sl d ) mutant mice, which lack expression of membrane bound form SCF, were used as recipients. As seen in the first experiment,
the number of undifferentiated spermatogonia was significantly higher in leuprorelin-treated than in the control group. Since
undifferentiated spermatogonia do not express the receptor of SCF, the present study clearly demonstrates that neither membrane-bound
nor secreted forms of SCF are involved in the mechanism of GnRH-analogue's effect on spermatogonial proliferation and/or survival.</description><identifier>ISSN: 0006-3363</identifier><identifier>EISSN: 1529-7268</identifier><identifier>DOI: 10.1095/biolreprod.102.013276</identifier><identifier>PMID: 12606404</identifier><identifier>CODEN: BIREBV</identifier><language>eng</language><publisher>Madison, WI: Society for the Study of Reproduction</publisher><subject>Animals ; Biological and medical sciences ; Body Weight - drug effects ; Fundamental and applied biological sciences. Psychology ; Germ Cells - drug effects ; Gonadotropin-Releasing Hormone - analogs & derivatives ; Gonadotropin-Releasing Hormone - pharmacology ; Green Fluorescent Proteins ; Hormone metabolism and regulation ; Immunohistochemistry ; Leuprolide - pharmacology ; Luminescent Proteins - metabolism ; Male ; Mammalian male genital system ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Microscopy, Confocal ; Organ Size - drug effects ; Proto-Oncogene Proteins c-kit - physiology ; Seminiferous Tubules - physiology ; Signal Transduction - physiology ; Sperm Count ; Spermatogenesis - genetics ; Spermatogonia - drug effects ; Spermatogonia - transplantation ; Stem Cell Factor - genetics ; Stem Cell Factor - physiology ; Vertebrates: reproduction</subject><ispartof>Biology of reproduction, 2003-06, Vol.68 (6), p.2304-2313</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15153028$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12606404$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>OHMURA, Masako</creatorcontrib><creatorcontrib>OGAWA, Takehiko</creatorcontrib><creatorcontrib>ONO, Michio</creatorcontrib><creatorcontrib>DEZAWA, Mari</creatorcontrib><creatorcontrib>HOSAKA, Masahiko</creatorcontrib><creatorcontrib>KUBOTA, Yoshinobu</creatorcontrib><creatorcontrib>SAWADA, Hajime</creatorcontrib><title>Increment of Murine Spermatogonial Cell Number by Gonadotropin-Releasing Hormone Analogue Is Independent of Stem Cell Factor c-kit Signal</title><title>Biology of reproduction</title><addtitle>Biol Reprod</addtitle><description>Recent studies have demonstrated that GnRH-analogues can stimulate regeneration of spermatogenesis of rats when administered
after testicular damages. Although the mechanism of this phenomenon has not been elucidated yet, stem cell factor (SCF) produced
by Sertoli cells was proposed to mediate the effects of GnRH-analogues on spermatogonial proliferation and/or survival. In
the present study, we quantitatively evaluated the proliferation of spermatogonia and addressed whether SCF mediates the effect
of GnRH-analogue on spermatogonial proliferation, using a novel approach combining spermatogonial transplantation and laser
confocal microscopic observation. In the first experiment, using wild-type mice as recipients for spermatogonial transplantation,
the number of donor spermatogonia per 100 Sertoli cells in each spermatogenic colony was significantly higher in the experimental
group of mice treated with leuprorelin, a GnRH-agonist, than that of the control group at 4 and 5 wk after transplantation.
In the second experiment, Steel/Steel dickie (Sl/Sl d ) mutant mice, which lack expression of membrane bound form SCF, were used as recipients. As seen in the first experiment,
the number of undifferentiated spermatogonia was significantly higher in leuprorelin-treated than in the control group. Since
undifferentiated spermatogonia do not express the receptor of SCF, the present study clearly demonstrates that neither membrane-bound
nor secreted forms of SCF are involved in the mechanism of GnRH-analogue's effect on spermatogonial proliferation and/or survival.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Body Weight - drug effects</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Germ Cells - drug effects</subject><subject>Gonadotropin-Releasing Hormone - analogs & derivatives</subject><subject>Gonadotropin-Releasing Hormone - pharmacology</subject><subject>Green Fluorescent Proteins</subject><subject>Hormone metabolism and regulation</subject><subject>Immunohistochemistry</subject><subject>Leuprolide - pharmacology</subject><subject>Luminescent Proteins - metabolism</subject><subject>Male</subject><subject>Mammalian male genital system</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Knockout</subject><subject>Microscopy, Confocal</subject><subject>Organ Size - drug effects</subject><subject>Proto-Oncogene Proteins c-kit - physiology</subject><subject>Seminiferous Tubules - physiology</subject><subject>Signal Transduction - physiology</subject><subject>Sperm Count</subject><subject>Spermatogenesis - genetics</subject><subject>Spermatogonia - drug effects</subject><subject>Spermatogonia - transplantation</subject><subject>Stem Cell Factor - genetics</subject><subject>Stem Cell Factor - physiology</subject><subject>Vertebrates: reproduction</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkNtO3DAQhi3UCpbDI1D5ptwFfIi9ySVacVgJqNQt15ETT7JufUjtRCsegbfG0i7iZqyRvvlm_CN0Sck1JbW4aU2wEcYYdO7ZNaGcLeURWlDB6mLJZPUNLQghsuBc8hN0mtJfQmjJGT9GJ5RJIktSLtD72ncRHPgJhx4_z9F4wJsRolNTGII3yuIVWItfZtdCxO0bfghe6TDFMBpf_AYLKhk_4McQXcjDt17ZMMyA1wmvvYYRctnrNxO4ve1edVOIuCv-mQlvzJBnztH3XtkEF4f3DL3e3_1ZPRZPvx7Wq9unYps_NRWyb6HVRHQqFyaWlSy5LusSKBVCUF0zUtK6Z1r3vGeyk7XkvWp5JQijZdXyM3S19-bs_s-QpsaZ1OWjlIcwp2bJWS1yPhn8cQDn1oFuxmicim_NZ3gZ-HkAVOqU7aPynUlfnKCCE1Z9bdyaYbszEZrklLVZy5vdbierRjaMZ-EHxWiOyA</recordid><startdate>20030601</startdate><enddate>20030601</enddate><creator>OHMURA, Masako</creator><creator>OGAWA, Takehiko</creator><creator>ONO, Michio</creator><creator>DEZAWA, Mari</creator><creator>HOSAKA, Masahiko</creator><creator>KUBOTA, Yoshinobu</creator><creator>SAWADA, Hajime</creator><general>Society for the Study of Reproduction</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20030601</creationdate><title>Increment of Murine Spermatogonial Cell Number by Gonadotropin-Releasing Hormone Analogue Is Independent of Stem Cell Factor c-kit Signal</title><author>OHMURA, Masako ; OGAWA, Takehiko ; ONO, Michio ; DEZAWA, Mari ; HOSAKA, Masahiko ; KUBOTA, Yoshinobu ; SAWADA, Hajime</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h268t-6fbebd05cad052578643d494e115551d920419f2ddf3f26c6963fab38502148b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Body Weight - drug effects</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Germ Cells - drug effects</topic><topic>Gonadotropin-Releasing Hormone - analogs & derivatives</topic><topic>Gonadotropin-Releasing Hormone - pharmacology</topic><topic>Green Fluorescent Proteins</topic><topic>Hormone metabolism and regulation</topic><topic>Immunohistochemistry</topic><topic>Leuprolide - pharmacology</topic><topic>Luminescent Proteins - metabolism</topic><topic>Male</topic><topic>Mammalian male genital system</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Knockout</topic><topic>Microscopy, Confocal</topic><topic>Organ Size - drug effects</topic><topic>Proto-Oncogene Proteins c-kit - physiology</topic><topic>Seminiferous Tubules - physiology</topic><topic>Signal Transduction - physiology</topic><topic>Sperm Count</topic><topic>Spermatogenesis - genetics</topic><topic>Spermatogonia - drug effects</topic><topic>Spermatogonia - transplantation</topic><topic>Stem Cell Factor - genetics</topic><topic>Stem Cell Factor - physiology</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>OHMURA, Masako</creatorcontrib><creatorcontrib>OGAWA, Takehiko</creatorcontrib><creatorcontrib>ONO, Michio</creatorcontrib><creatorcontrib>DEZAWA, Mari</creatorcontrib><creatorcontrib>HOSAKA, Masahiko</creatorcontrib><creatorcontrib>KUBOTA, Yoshinobu</creatorcontrib><creatorcontrib>SAWADA, Hajime</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>OHMURA, Masako</au><au>OGAWA, Takehiko</au><au>ONO, Michio</au><au>DEZAWA, Mari</au><au>HOSAKA, Masahiko</au><au>KUBOTA, Yoshinobu</au><au>SAWADA, Hajime</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Increment of Murine Spermatogonial Cell Number by Gonadotropin-Releasing Hormone Analogue Is Independent of Stem Cell Factor c-kit Signal</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>2003-06-01</date><risdate>2003</risdate><volume>68</volume><issue>6</issue><spage>2304</spage><epage>2313</epage><pages>2304-2313</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><coden>BIREBV</coden><abstract>Recent studies have demonstrated that GnRH-analogues can stimulate regeneration of spermatogenesis of rats when administered
after testicular damages. Although the mechanism of this phenomenon has not been elucidated yet, stem cell factor (SCF) produced
by Sertoli cells was proposed to mediate the effects of GnRH-analogues on spermatogonial proliferation and/or survival. In
the present study, we quantitatively evaluated the proliferation of spermatogonia and addressed whether SCF mediates the effect
of GnRH-analogue on spermatogonial proliferation, using a novel approach combining spermatogonial transplantation and laser
confocal microscopic observation. In the first experiment, using wild-type mice as recipients for spermatogonial transplantation,
the number of donor spermatogonia per 100 Sertoli cells in each spermatogenic colony was significantly higher in the experimental
group of mice treated with leuprorelin, a GnRH-agonist, than that of the control group at 4 and 5 wk after transplantation.
In the second experiment, Steel/Steel dickie (Sl/Sl d ) mutant mice, which lack expression of membrane bound form SCF, were used as recipients. As seen in the first experiment,
the number of undifferentiated spermatogonia was significantly higher in leuprorelin-treated than in the control group. Since
undifferentiated spermatogonia do not express the receptor of SCF, the present study clearly demonstrates that neither membrane-bound
nor secreted forms of SCF are involved in the mechanism of GnRH-analogue's effect on spermatogonial proliferation and/or survival.</abstract><cop>Madison, WI</cop><pub>Society for the Study of Reproduction</pub><pmid>12606404</pmid><doi>10.1095/biolreprod.102.013276</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-3363 |
| ispartof | Biology of reproduction, 2003-06, Vol.68 (6), p.2304-2313 |
| issn | 0006-3363 1529-7268 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73295260 |
| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; BioOne Complete |
| subjects | Animals Biological and medical sciences Body Weight - drug effects Fundamental and applied biological sciences. Psychology Germ Cells - drug effects Gonadotropin-Releasing Hormone - analogs & derivatives Gonadotropin-Releasing Hormone - pharmacology Green Fluorescent Proteins Hormone metabolism and regulation Immunohistochemistry Leuprolide - pharmacology Luminescent Proteins - metabolism Male Mammalian male genital system Mice Mice, Inbred C57BL Mice, Knockout Microscopy, Confocal Organ Size - drug effects Proto-Oncogene Proteins c-kit - physiology Seminiferous Tubules - physiology Signal Transduction - physiology Sperm Count Spermatogenesis - genetics Spermatogonia - drug effects Spermatogonia - transplantation Stem Cell Factor - genetics Stem Cell Factor - physiology Vertebrates: reproduction |
| title | Increment of Murine Spermatogonial Cell Number by Gonadotropin-Releasing Hormone Analogue Is Independent of Stem Cell Factor c-kit Signal |
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