G-Quadruplex-Specific Peptide−Hemicyanine Ligands by Partial Combinatorial Selection

G-Quadruplex-Specific Peptide−Hemicyanine Ligands by Partial Combinatorial Selection

G-quadruplex DNA-specific ligands were generated using a combinatorial selection of tetrapeptides which were then conjugated to a hemicyanine scaffold. The heterocycle enhanced peptide binding affinity by ∼1000-fold to give ligands with near micromolar affinity and >40-fold discrimination for qua...

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Veröffentlicht in:Journal of the American Chemical Society 2003-05, Vol.125 (19), p.5594-5595
Hauptverfasser: Schouten, James A, Ladame, Sylvain, Mason, Stephen J, Cooper, Matthew A, Balasubramanian, Shankar
Format: Artikel
Sprache:eng
Schlagworte:
Biological and medical sciences
Carbocyanines - chemistry
Coloring Agents - chemistry
Combinatorial Chemistry Techniques - methods
DNA - chemistry
DNA - metabolism
Fundamental and applied biological sciences. Psychology
G-Quadruplexes
Interactions. Associations
Intermolecular phenomena
Kinetics
Ligands
Molecular biophysics
Oligopeptides - chemistry
Oligopeptides - metabolism
Substrate Specificity
Surface Plasmon Resonance
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Zusammenfassung:G-quadruplex DNA-specific ligands were generated using a combinatorial selection of tetrapeptides which were then conjugated to a hemicyanine scaffold. The heterocycle enhanced peptide binding affinity by ∼1000-fold to give ligands with near micromolar affinity and >40-fold discrimination for quadruplex DNA over duplex.
ISSN:0002-7863
1520-5126
DOI:10.1021/ja029356w