Study on physical properties and nerve cell affinity of composite films from chitosan and gelatin solutions
A series of chitosan–gelatin composite films was prepared by varying the ratio of constituents. FT-IR and X-ray analysis showed good compatibility between these two biopolymers. Differential scanning calorimetry (DSC) analysis indicated that the water take-up of chitosan film increased when blended...
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| Veröffentlicht in: | Biomaterials 2003-08, Vol.24 (17), p.2871-2880 |
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| container_title | Biomaterials |
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| creator | Cheng, Mingyu Deng, Jinguang Yang, Fei Gong, Yandao Zhao, Nanming Zhang, Xiufang |
| description | A series of chitosan–gelatin composite films was prepared by varying the ratio of constituents. FT-IR and X-ray analysis showed good compatibility between these two biopolymers. Differential scanning calorimetry (DSC) analysis indicated that the water take-up of chitosan film increased when blended with gelatin. Composite film exhibited a lower Young's modulus and a higher percentage of elongation-at-break compared with chitosan film, especially in wet state. All composite films were hydrophilic materials with water contact angles ranging from 55° to 65°. The results obtained from ELISA indicated the adsorption amount of fibronectin on composite films was much higher than on chitosan film. PC12 cells culture was used to evaluate the nerve cell affinity of materials. The cells cultured on the composite film with 60
wt% gelatin differentiated more rapidly and extended longer neurites than on chitosan film. The results suggest that the soft and elastic complex of chitosan and gelatin, which has better nerve cell affinity compared to chitosan, is a promising candidate biomaterial for nerve regeneration. |
| doi_str_mv | 10.1016/S0142-9612(03)00117-0 |
| format | Article |
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wt% gelatin differentiated more rapidly and extended longer neurites than on chitosan film. The results suggest that the soft and elastic complex of chitosan and gelatin, which has better nerve cell affinity compared to chitosan, is a promising candidate biomaterial for nerve regeneration.</description><identifier>ISSN: 0142-9612</identifier><identifier>EISSN: 1878-5905</identifier><identifier>DOI: 10.1016/S0142-9612(03)00117-0</identifier><identifier>PMID: 12742725</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Adsorption ; Animals ; Biocompatible Materials - chemical synthesis ; Biocompatible Materials - chemistry ; Cell Adhesion - physiology ; Cell Division - physiology ; Chitin - analogs & derivatives ; Chitin - chemical synthesis ; Chitin - chemistry ; Chitosan ; Composite film ; Elasticity ; Fibronectins - chemistry ; Gelatin ; Gelatin - chemical synthesis ; Gelatin - chemistry ; Manufactured Materials ; Materials Testing ; Mechanical property ; Nerve cell affinity ; Nerve regeneration ; Nerve Regeneration - physiology ; Neurites - physiology ; Neurites - ultrastructure ; Neurons - cytology ; Neurons - physiology ; PC12 Cells ; Protein Binding ; Rats ; Surface Properties ; Tensile Strength ; Tissue Engineering - methods</subject><ispartof>Biomaterials, 2003-08, Vol.24 (17), p.2871-2880</ispartof><rights>2003 Elsevier Science Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c489t-936ec56d0d4d480194866a5d470a042d39b3e87202beea3c2fd9d998a2a2abe23</citedby><cites>FETCH-LOGICAL-c489t-936ec56d0d4d480194866a5d470a042d39b3e87202beea3c2fd9d998a2a2abe23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0142-9612(03)00117-0$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12742725$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cheng, Mingyu</creatorcontrib><creatorcontrib>Deng, Jinguang</creatorcontrib><creatorcontrib>Yang, Fei</creatorcontrib><creatorcontrib>Gong, Yandao</creatorcontrib><creatorcontrib>Zhao, Nanming</creatorcontrib><creatorcontrib>Zhang, Xiufang</creatorcontrib><title>Study on physical properties and nerve cell affinity of composite films from chitosan and gelatin solutions</title><title>Biomaterials</title><addtitle>Biomaterials</addtitle><description>A series of chitosan–gelatin composite films was prepared by varying the ratio of constituents. FT-IR and X-ray analysis showed good compatibility between these two biopolymers. Differential scanning calorimetry (DSC) analysis indicated that the water take-up of chitosan film increased when blended with gelatin. Composite film exhibited a lower Young's modulus and a higher percentage of elongation-at-break compared with chitosan film, especially in wet state. All composite films were hydrophilic materials with water contact angles ranging from 55° to 65°. The results obtained from ELISA indicated the adsorption amount of fibronectin on composite films was much higher than on chitosan film. PC12 cells culture was used to evaluate the nerve cell affinity of materials. The cells cultured on the composite film with 60
wt% gelatin differentiated more rapidly and extended longer neurites than on chitosan film. The results suggest that the soft and elastic complex of chitosan and gelatin, which has better nerve cell affinity compared to chitosan, is a promising candidate biomaterial for nerve regeneration.</description><subject>Adsorption</subject><subject>Animals</subject><subject>Biocompatible Materials - chemical synthesis</subject><subject>Biocompatible Materials - chemistry</subject><subject>Cell Adhesion - physiology</subject><subject>Cell Division - physiology</subject><subject>Chitin - analogs & derivatives</subject><subject>Chitin - chemical synthesis</subject><subject>Chitin - chemistry</subject><subject>Chitosan</subject><subject>Composite film</subject><subject>Elasticity</subject><subject>Fibronectins - chemistry</subject><subject>Gelatin</subject><subject>Gelatin - chemical synthesis</subject><subject>Gelatin - chemistry</subject><subject>Manufactured Materials</subject><subject>Materials Testing</subject><subject>Mechanical property</subject><subject>Nerve cell affinity</subject><subject>Nerve regeneration</subject><subject>Nerve Regeneration - physiology</subject><subject>Neurites - physiology</subject><subject>Neurites - ultrastructure</subject><subject>Neurons - cytology</subject><subject>Neurons - physiology</subject><subject>PC12 Cells</subject><subject>Protein Binding</subject><subject>Rats</subject><subject>Surface Properties</subject><subject>Tensile Strength</subject><subject>Tissue Engineering - methods</subject><issn>0142-9612</issn><issn>1878-5905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2PFCEQhonRuOPoT9BwMnpoLWiabk5ms_Er2cTD6pkwUO2i3dACvcn8e5mP6HHCAQ5PUfXWQ8hLBu8YMPn-DpjgjZKMv4H2LQBjfQOPyIYN_dB0CrrHZPMPuSLPcv5VIQGCPyVXjPeC97zbkN93ZXV7GgNd7vfZWzPRJcUFU_GYqQmOBkwPSC1OEzXj6IMvFR-pjfMSsy9IRz_NmY4pztTe-xKzCcfCnziZ4gPNcVqLjyE_J09GM2V8cb635Menj99vvjS33z5_vbm-bawYVGlUK9F20oETTgzAlBikNJ0TPZg6vmvVrsWh58B3iKa1fHTKKTUYXs8Oebslr0__1iR_VsxFzz4fApiAcc26b2t8kOIiyAeAthPqIliXLoSUfQW7E2hTzDnhqJfkZ5P2moE-eNNHb_ogRUOrj97qY0tenRusuxnd_6qzqAp8OAFYF_fgMelsPQaLzie0RbvoL7T4C3gPqIo</recordid><startdate>20030801</startdate><enddate>20030801</enddate><creator>Cheng, Mingyu</creator><creator>Deng, Jinguang</creator><creator>Yang, Fei</creator><creator>Gong, Yandao</creator><creator>Zhao, Nanming</creator><creator>Zhang, Xiufang</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>F28</scope><scope>7X8</scope></search><sort><creationdate>20030801</creationdate><title>Study on physical properties and nerve cell affinity of composite films from chitosan and gelatin solutions</title><author>Cheng, Mingyu ; Deng, Jinguang ; Yang, Fei ; Gong, Yandao ; Zhao, Nanming ; Zhang, Xiufang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c489t-936ec56d0d4d480194866a5d470a042d39b3e87202beea3c2fd9d998a2a2abe23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Adsorption</topic><topic>Animals</topic><topic>Biocompatible Materials - chemical synthesis</topic><topic>Biocompatible Materials - chemistry</topic><topic>Cell Adhesion - physiology</topic><topic>Cell Division - physiology</topic><topic>Chitin - analogs & derivatives</topic><topic>Chitin - chemical synthesis</topic><topic>Chitin - chemistry</topic><topic>Chitosan</topic><topic>Composite film</topic><topic>Elasticity</topic><topic>Fibronectins - chemistry</topic><topic>Gelatin</topic><topic>Gelatin - chemical synthesis</topic><topic>Gelatin - chemistry</topic><topic>Manufactured Materials</topic><topic>Materials Testing</topic><topic>Mechanical property</topic><topic>Nerve cell affinity</topic><topic>Nerve regeneration</topic><topic>Nerve Regeneration - physiology</topic><topic>Neurites - physiology</topic><topic>Neurites - ultrastructure</topic><topic>Neurons - cytology</topic><topic>Neurons - physiology</topic><topic>PC12 Cells</topic><topic>Protein Binding</topic><topic>Rats</topic><topic>Surface Properties</topic><topic>Tensile Strength</topic><topic>Tissue Engineering - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cheng, Mingyu</creatorcontrib><creatorcontrib>Deng, Jinguang</creatorcontrib><creatorcontrib>Yang, Fei</creatorcontrib><creatorcontrib>Gong, Yandao</creatorcontrib><creatorcontrib>Zhao, Nanming</creatorcontrib><creatorcontrib>Zhang, Xiufang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>MEDLINE - Academic</collection><jtitle>Biomaterials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cheng, Mingyu</au><au>Deng, Jinguang</au><au>Yang, Fei</au><au>Gong, Yandao</au><au>Zhao, Nanming</au><au>Zhang, Xiufang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Study on physical properties and nerve cell affinity of composite films from chitosan and gelatin solutions</atitle><jtitle>Biomaterials</jtitle><addtitle>Biomaterials</addtitle><date>2003-08-01</date><risdate>2003</risdate><volume>24</volume><issue>17</issue><spage>2871</spage><epage>2880</epage><pages>2871-2880</pages><issn>0142-9612</issn><eissn>1878-5905</eissn><abstract>A series of chitosan–gelatin composite films was prepared by varying the ratio of constituents. FT-IR and X-ray analysis showed good compatibility between these two biopolymers. Differential scanning calorimetry (DSC) analysis indicated that the water take-up of chitosan film increased when blended with gelatin. Composite film exhibited a lower Young's modulus and a higher percentage of elongation-at-break compared with chitosan film, especially in wet state. All composite films were hydrophilic materials with water contact angles ranging from 55° to 65°. The results obtained from ELISA indicated the adsorption amount of fibronectin on composite films was much higher than on chitosan film. PC12 cells culture was used to evaluate the nerve cell affinity of materials. The cells cultured on the composite film with 60
wt% gelatin differentiated more rapidly and extended longer neurites than on chitosan film. The results suggest that the soft and elastic complex of chitosan and gelatin, which has better nerve cell affinity compared to chitosan, is a promising candidate biomaterial for nerve regeneration.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>12742725</pmid><doi>10.1016/S0142-9612(03)00117-0</doi><tpages>10</tpages></addata></record> |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Adsorption Animals Biocompatible Materials - chemical synthesis Biocompatible Materials - chemistry Cell Adhesion - physiology Cell Division - physiology Chitin - analogs & derivatives Chitin - chemical synthesis Chitin - chemistry Chitosan Composite film Elasticity Fibronectins - chemistry Gelatin Gelatin - chemical synthesis Gelatin - chemistry Manufactured Materials Materials Testing Mechanical property Nerve cell affinity Nerve regeneration Nerve Regeneration - physiology Neurites - physiology Neurites - ultrastructure Neurons - cytology Neurons - physiology PC12 Cells Protein Binding Rats Surface Properties Tensile Strength Tissue Engineering - methods |
| title | Study on physical properties and nerve cell affinity of composite films from chitosan and gelatin solutions |
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