Genome-wide expression analyses: Metabolic adaptation of Saccharomyces cerevisiae to high sugar stress
The transcriptional response of laboratory strains of Saccharomyces cerevisiae to salt or sorbitol stress has been well studied. These studies have yielded valuable data on how the yeast adapts to these stress conditions. However, S. cerevisiae is a saccharophilic fungus and in its natural environme...
Gespeichert in:
Veröffentlicht in: | FEMS yeast research 2003-06, Vol.3 (4), p.375-399 |
---|---|
Hauptverfasser: | , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 399 |
---|---|
container_issue | 4 |
container_start_page | 375 |
container_title | FEMS yeast research |
container_volume | 3 |
creator | Erasmus, Daniel J van der Merwe, George K van Vuuren, Hennie J.J |
description | The transcriptional response of laboratory strains of
Saccharomyces cerevisiae to salt or sorbitol stress has been well studied. These studies have yielded valuable data on how the yeast adapts to these stress conditions. However,
S. cerevisiae is a saccharophilic fungus and in its natural environment this yeast encounters high concentrations of sugars. For the production of dessert wines, the sugar concentration may be as high as 50% (w/v). The metabolic pathways in
S. cerevisiae under these fermentation conditions have not been studied and the transcriptional response of this yeast to sugar stress has not been investigated. High-density DNA microarrays showed that the transcription of 589 genes in an industrial strain of
S. cerevisiae were affected more than two-fold in grape juice containing 40% (w/v) sugars (equimolar amounts of glucose and fructose). High sugar stress up-regulated the glycolytic and pentose phosphate pathway genes. The
PDC6 gene, previously thought to encode a minor isozyme of pyruvate decarboxylase, was highly induced under these conditions. Gene expression profiles indicate that the oxidative and non-oxidative branches of the pentose phosphate pathway were up-regulated and might be used to shunt more glucose-6-phosphate and fructose-6-phosphate, respectively, from the glycolytic pathway into the pentose phosphate pathway. Structural genes involved in the formation of acetic acid from acetaldehyde, and succinic acid from glutamate, were also up-regulated. Genes involved in de novo biosynthesis of purines, pyrimidines, histidine and lysine were down-regulated by sugar stress. |
doi_str_mv | 10.1016/S1567-1356(02)00203-9 |
format | Article |
fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_73273857</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><oup_id>10.1016/S1567-1356(02)00203-9</oup_id><els_id>S1567135602002039</els_id><sourcerecordid>73273857</sourcerecordid><originalsourceid>FETCH-LOGICAL-e4135-2fd0e695de6429e9c16fe24d07eb55eae757965a2311f453322318b3e6350dd03</originalsourceid><addsrcrecordid>eNqFkVFrFDEUhQdRbK3-BCUgiD6M3iSTZMYXkdJWoSJYffApZJM73ZSZyZjMtO6_b2Z3VRTRp4Tc851czimKxxReUqDy1QUVUpWUC_kc2AsABrxs7hSH-2dZ3f15F_KgeJDSFQBVAPX94oAyVdUg4LBoz3AIPZY33iHB72PElHwYiBlMt0mYXpMPOJlV6LwlxplxMtMyDi25MNauTQz9xmIiFiNe--QNkimQtb9ckzRfmkjStFg-LO61pkv4aH8eFV9OTz4fvyvPP569P357XmKV9yxZ6wBlIxzKijXYWCpbZJUDhSsh0KASqpHCME5pWwnOWb7VK46SC3AO-FHxbOc7xvBtxjTp3ieLXWcGDHPSijPFa6H-K6S1yp5icXz6h_AqzDGnkzTjedWc_NbuyV41r3p0eoy-N3GjfwSdBc1OcOM73Pyag17a1Ns29VKVBqa3bepGn379xJXILOzYMI9_J8vfyLLJyJsdgjnsa49RJ-txsOh8RDtpF_y__-a3pqGymQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2369500257</pqid></control><display><type>article</type><title>Genome-wide expression analyses: Metabolic adaptation of Saccharomyces cerevisiae to high sugar stress</title><source>MEDLINE</source><source>Access via Oxford University Press (Open Access Collection)</source><source>Access via Wiley Online Library</source><source>Alma/SFX Local Collection</source><creator>Erasmus, Daniel J ; van der Merwe, George K ; van Vuuren, Hennie J.J</creator><creatorcontrib>Erasmus, Daniel J ; van der Merwe, George K ; van Vuuren, Hennie J.J</creatorcontrib><description>The transcriptional response of laboratory strains of
Saccharomyces cerevisiae to salt or sorbitol stress has been well studied. These studies have yielded valuable data on how the yeast adapts to these stress conditions. However,
S. cerevisiae is a saccharophilic fungus and in its natural environment this yeast encounters high concentrations of sugars. For the production of dessert wines, the sugar concentration may be as high as 50% (w/v). The metabolic pathways in
S. cerevisiae under these fermentation conditions have not been studied and the transcriptional response of this yeast to sugar stress has not been investigated. High-density DNA microarrays showed that the transcription of 589 genes in an industrial strain of
S. cerevisiae were affected more than two-fold in grape juice containing 40% (w/v) sugars (equimolar amounts of glucose and fructose). High sugar stress up-regulated the glycolytic and pentose phosphate pathway genes. The
PDC6 gene, previously thought to encode a minor isozyme of pyruvate decarboxylase, was highly induced under these conditions. Gene expression profiles indicate that the oxidative and non-oxidative branches of the pentose phosphate pathway were up-regulated and might be used to shunt more glucose-6-phosphate and fructose-6-phosphate, respectively, from the glycolytic pathway into the pentose phosphate pathway. Structural genes involved in the formation of acetic acid from acetaldehyde, and succinic acid from glutamate, were also up-regulated. Genes involved in de novo biosynthesis of purines, pyrimidines, histidine and lysine were down-regulated by sugar stress.</description><identifier>ISSN: 1567-1356</identifier><identifier>EISSN: 1567-1364</identifier><identifier>DOI: 10.1016/S1567-1356(02)00203-9</identifier><identifier>PMID: 12748050</identifier><language>eng</language><publisher>Oxford, UK: Elsevier B.V</publisher><subject>Acetaldehyde ; Acetaldehyde - metabolism ; Acetic acid ; Acetic Acid - metabolism ; Carbohydrate Metabolism ; DNA array analysis ; DNA microarrays ; Fermentation ; Fructose ; Fructose-6-phosphate ; Fungi ; Gene expression ; Gene Expression Profiling ; Gene Expression Regulation, Fungal ; Genomes ; Glutamic Acid - metabolism ; Glycolysis ; Glycolysis - genetics ; Glycolysis - physiology ; Histidine ; Lysine ; Metabolic pathways ; Metabolism ; Oligonucleotide Array Sequence Analysis ; Osmotic stress ; Pentose phosphate pathway ; Pentose Phosphate Pathway - genetics ; Pentose Phosphate Pathway - physiology ; Purines ; Pyrimidines ; Pyruvate decarboxylase ; Pyruvic acid ; RNA, Fungal - genetics ; RNA, Fungal - metabolism ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae - metabolism ; Sorbitol ; Succinic acid ; Succinic Acid - metabolism ; Sugar ; Transcription ; Transcription, Genetic - physiology ; Wine ; Yeast</subject><ispartof>FEMS yeast research, 2003-06, Vol.3 (4), p.375-399</ispartof><rights>2002 Federation of European Microbiological Societies</rights><rights>2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved. 2003</rights><rights>2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2FS1567-1356%2802%2900203-9$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1016%2FS1567-1356%2802%2900203-9$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12748050$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Erasmus, Daniel J</creatorcontrib><creatorcontrib>van der Merwe, George K</creatorcontrib><creatorcontrib>van Vuuren, Hennie J.J</creatorcontrib><title>Genome-wide expression analyses: Metabolic adaptation of Saccharomyces cerevisiae to high sugar stress</title><title>FEMS yeast research</title><addtitle>FEMS Yeast Res</addtitle><description>The transcriptional response of laboratory strains of
Saccharomyces cerevisiae to salt or sorbitol stress has been well studied. These studies have yielded valuable data on how the yeast adapts to these stress conditions. However,
S. cerevisiae is a saccharophilic fungus and in its natural environment this yeast encounters high concentrations of sugars. For the production of dessert wines, the sugar concentration may be as high as 50% (w/v). The metabolic pathways in
S. cerevisiae under these fermentation conditions have not been studied and the transcriptional response of this yeast to sugar stress has not been investigated. High-density DNA microarrays showed that the transcription of 589 genes in an industrial strain of
S. cerevisiae were affected more than two-fold in grape juice containing 40% (w/v) sugars (equimolar amounts of glucose and fructose). High sugar stress up-regulated the glycolytic and pentose phosphate pathway genes. The
PDC6 gene, previously thought to encode a minor isozyme of pyruvate decarboxylase, was highly induced under these conditions. Gene expression profiles indicate that the oxidative and non-oxidative branches of the pentose phosphate pathway were up-regulated and might be used to shunt more glucose-6-phosphate and fructose-6-phosphate, respectively, from the glycolytic pathway into the pentose phosphate pathway. Structural genes involved in the formation of acetic acid from acetaldehyde, and succinic acid from glutamate, were also up-regulated. Genes involved in de novo biosynthesis of purines, pyrimidines, histidine and lysine were down-regulated by sugar stress.</description><subject>Acetaldehyde</subject><subject>Acetaldehyde - metabolism</subject><subject>Acetic acid</subject><subject>Acetic Acid - metabolism</subject><subject>Carbohydrate Metabolism</subject><subject>DNA array analysis</subject><subject>DNA microarrays</subject><subject>Fermentation</subject><subject>Fructose</subject><subject>Fructose-6-phosphate</subject><subject>Fungi</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Fungal</subject><subject>Genomes</subject><subject>Glutamic Acid - metabolism</subject><subject>Glycolysis</subject><subject>Glycolysis - genetics</subject><subject>Glycolysis - physiology</subject><subject>Histidine</subject><subject>Lysine</subject><subject>Metabolic pathways</subject><subject>Metabolism</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Osmotic stress</subject><subject>Pentose phosphate pathway</subject><subject>Pentose Phosphate Pathway - genetics</subject><subject>Pentose Phosphate Pathway - physiology</subject><subject>Purines</subject><subject>Pyrimidines</subject><subject>Pyruvate decarboxylase</subject><subject>Pyruvic acid</subject><subject>RNA, Fungal - genetics</subject><subject>RNA, Fungal - metabolism</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae - metabolism</subject><subject>Sorbitol</subject><subject>Succinic acid</subject><subject>Succinic Acid - metabolism</subject><subject>Sugar</subject><subject>Transcription</subject><subject>Transcription, Genetic - physiology</subject><subject>Wine</subject><subject>Yeast</subject><issn>1567-1356</issn><issn>1567-1364</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkVFrFDEUhQdRbK3-BCUgiD6M3iSTZMYXkdJWoSJYffApZJM73ZSZyZjMtO6_b2Z3VRTRp4Tc851czimKxxReUqDy1QUVUpWUC_kc2AsABrxs7hSH-2dZ3f15F_KgeJDSFQBVAPX94oAyVdUg4LBoz3AIPZY33iHB72PElHwYiBlMt0mYXpMPOJlV6LwlxplxMtMyDi25MNauTQz9xmIiFiNe--QNkimQtb9ckzRfmkjStFg-LO61pkv4aH8eFV9OTz4fvyvPP569P357XmKV9yxZ6wBlIxzKijXYWCpbZJUDhSsh0KASqpHCME5pWwnOWb7VK46SC3AO-FHxbOc7xvBtxjTp3ieLXWcGDHPSijPFa6H-K6S1yp5icXz6h_AqzDGnkzTjedWc_NbuyV41r3p0eoy-N3GjfwSdBc1OcOM73Pyag17a1Ns29VKVBqa3bepGn379xJXILOzYMI9_J8vfyLLJyJsdgjnsa49RJ-txsOh8RDtpF_y__-a3pqGymQ</recordid><startdate>200306</startdate><enddate>200306</enddate><creator>Erasmus, Daniel J</creator><creator>van der Merwe, George K</creator><creator>van Vuuren, Hennie J.J</creator><general>Elsevier B.V</general><general>Blackwell Publishing Ltd</general><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>200306</creationdate><title>Genome-wide expression analyses: Metabolic adaptation of Saccharomyces cerevisiae to high sugar stress</title><author>Erasmus, Daniel J ; van der Merwe, George K ; van Vuuren, Hennie J.J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e4135-2fd0e695de6429e9c16fe24d07eb55eae757965a2311f453322318b3e6350dd03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Acetaldehyde</topic><topic>Acetaldehyde - metabolism</topic><topic>Acetic acid</topic><topic>Acetic Acid - metabolism</topic><topic>Carbohydrate Metabolism</topic><topic>DNA array analysis</topic><topic>DNA microarrays</topic><topic>Fermentation</topic><topic>Fructose</topic><topic>Fructose-6-phosphate</topic><topic>Fungi</topic><topic>Gene expression</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Fungal</topic><topic>Genomes</topic><topic>Glutamic Acid - metabolism</topic><topic>Glycolysis</topic><topic>Glycolysis - genetics</topic><topic>Glycolysis - physiology</topic><topic>Histidine</topic><topic>Lysine</topic><topic>Metabolic pathways</topic><topic>Metabolism</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Osmotic stress</topic><topic>Pentose phosphate pathway</topic><topic>Pentose Phosphate Pathway - genetics</topic><topic>Pentose Phosphate Pathway - physiology</topic><topic>Purines</topic><topic>Pyrimidines</topic><topic>Pyruvate decarboxylase</topic><topic>Pyruvic acid</topic><topic>RNA, Fungal - genetics</topic><topic>RNA, Fungal - metabolism</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Saccharomyces cerevisiae - metabolism</topic><topic>Sorbitol</topic><topic>Succinic acid</topic><topic>Succinic Acid - metabolism</topic><topic>Sugar</topic><topic>Transcription</topic><topic>Transcription, Genetic - physiology</topic><topic>Wine</topic><topic>Yeast</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Erasmus, Daniel J</creatorcontrib><creatorcontrib>van der Merwe, George K</creatorcontrib><creatorcontrib>van Vuuren, Hennie J.J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS yeast research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Erasmus, Daniel J</au><au>van der Merwe, George K</au><au>van Vuuren, Hennie J.J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genome-wide expression analyses: Metabolic adaptation of Saccharomyces cerevisiae to high sugar stress</atitle><jtitle>FEMS yeast research</jtitle><addtitle>FEMS Yeast Res</addtitle><date>2003-06</date><risdate>2003</risdate><volume>3</volume><issue>4</issue><spage>375</spage><epage>399</epage><pages>375-399</pages><issn>1567-1356</issn><eissn>1567-1364</eissn><abstract>The transcriptional response of laboratory strains of
Saccharomyces cerevisiae to salt or sorbitol stress has been well studied. These studies have yielded valuable data on how the yeast adapts to these stress conditions. However,
S. cerevisiae is a saccharophilic fungus and in its natural environment this yeast encounters high concentrations of sugars. For the production of dessert wines, the sugar concentration may be as high as 50% (w/v). The metabolic pathways in
S. cerevisiae under these fermentation conditions have not been studied and the transcriptional response of this yeast to sugar stress has not been investigated. High-density DNA microarrays showed that the transcription of 589 genes in an industrial strain of
S. cerevisiae were affected more than two-fold in grape juice containing 40% (w/v) sugars (equimolar amounts of glucose and fructose). High sugar stress up-regulated the glycolytic and pentose phosphate pathway genes. The
PDC6 gene, previously thought to encode a minor isozyme of pyruvate decarboxylase, was highly induced under these conditions. Gene expression profiles indicate that the oxidative and non-oxidative branches of the pentose phosphate pathway were up-regulated and might be used to shunt more glucose-6-phosphate and fructose-6-phosphate, respectively, from the glycolytic pathway into the pentose phosphate pathway. Structural genes involved in the formation of acetic acid from acetaldehyde, and succinic acid from glutamate, were also up-regulated. Genes involved in de novo biosynthesis of purines, pyrimidines, histidine and lysine were down-regulated by sugar stress.</abstract><cop>Oxford, UK</cop><pub>Elsevier B.V</pub><pmid>12748050</pmid><doi>10.1016/S1567-1356(02)00203-9</doi><tpages>25</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 1567-1356 |
ispartof | FEMS yeast research, 2003-06, Vol.3 (4), p.375-399 |
issn | 1567-1356 1567-1364 |
language | eng |
recordid | cdi_proquest_miscellaneous_73273857 |
source | MEDLINE; Access via Oxford University Press (Open Access Collection); Access via Wiley Online Library; Alma/SFX Local Collection |
subjects | Acetaldehyde Acetaldehyde - metabolism Acetic acid Acetic Acid - metabolism Carbohydrate Metabolism DNA array analysis DNA microarrays Fermentation Fructose Fructose-6-phosphate Fungi Gene expression Gene Expression Profiling Gene Expression Regulation, Fungal Genomes Glutamic Acid - metabolism Glycolysis Glycolysis - genetics Glycolysis - physiology Histidine Lysine Metabolic pathways Metabolism Oligonucleotide Array Sequence Analysis Osmotic stress Pentose phosphate pathway Pentose Phosphate Pathway - genetics Pentose Phosphate Pathway - physiology Purines Pyrimidines Pyruvate decarboxylase Pyruvic acid RNA, Fungal - genetics RNA, Fungal - metabolism Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism Sorbitol Succinic acid Succinic Acid - metabolism Sugar Transcription Transcription, Genetic - physiology Wine Yeast |
title | Genome-wide expression analyses: Metabolic adaptation of Saccharomyces cerevisiae to high sugar stress |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T12%3A11%3A41IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Genome-wide%20expression%20analyses:%20Metabolic%20adaptation%20of%20Saccharomyces%20cerevisiae%20to%20high%20sugar%20stress&rft.jtitle=FEMS%20yeast%20research&rft.au=Erasmus,%20Daniel%20J&rft.date=2003-06&rft.volume=3&rft.issue=4&rft.spage=375&rft.epage=399&rft.pages=375-399&rft.issn=1567-1356&rft.eissn=1567-1364&rft_id=info:doi/10.1016/S1567-1356(02)00203-9&rft_dat=%3Cproquest_pubme%3E73273857%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2369500257&rft_id=info:pmid/12748050&rft_oup_id=10.1016/S1567-1356(02)00203-9&rft_els_id=S1567135602002039&rfr_iscdi=true |