Expression and phosphorylation of the replication regulator protein geminin
It has been described that the replication regulator protein geminin is rapidly degraded at the end of mitosis and newly expressed at the beginning of the next S phase in the metazoan cell cycle. We have performed experiments to investigate the synthesis of geminin in cycling human HeLa cells. The l...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2003-05, Vol.305 (2), p.412-420 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Kulartz, Monika Kreitz, Sandra Hiller, Ekkehard Damoc, Elena-Catalina Przybylski, Michael Knippers, Rolf |
| description | It has been described that the replication regulator protein geminin is rapidly degraded at the end of mitosis and newly expressed at the beginning of the next S phase in the metazoan cell cycle. We have performed experiments to investigate the synthesis of geminin in cycling human HeLa cells. The levels of geminin-mRNA vary only modestly during the cell cycle with a 2–3-fold higher mRNA level at the G1/S phase transition, whereas newly synthesized geminin can only be detected in post-G1 phases. Surprisingly, geminin, once synthesized, does not remain stable, but is turned over during S phase with a half-life of 3–4
h. We also show that geminin becomes phosphorylated as S phase proceeds and identify by MALDI mass spectrometry two specific major phosphorylation sites. |
| doi_str_mv | 10.1016/S0006-291X(03)00773-3 |
| format | Article |
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h. We also show that geminin becomes phosphorylated as S phase proceeds and identify by MALDI mass spectrometry two specific major phosphorylation sites.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/S0006-291X(03)00773-3</identifier><identifier>PMID: 12745091</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Amino Acid Sequence ; Cell cycle ; Cell Cycle Proteins - biosynthesis ; Cell Cycle Proteins - chemistry ; Cell Cycle Proteins - genetics ; Cell Cycle Proteins - metabolism ; DNA Replication ; Geminin ; HeLa Cells ; Humans ; Kinetics ; Mass spectrometry ; Molecular Sequence Data ; Phosphorylation ; Replication–regulation ; RNA, Messenger - biosynthesis ; S Phase ; Transcription, Genetic ; Turnover</subject><ispartof>Biochemical and biophysical research communications, 2003-05, Vol.305 (2), p.412-420</ispartof><rights>2003 Elsevier Science (USA)</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c458t-2764e09f3b0eb2c97414b473681717fa9f96864c0835e0bfbf130263398957543</citedby><cites>FETCH-LOGICAL-c458t-2764e09f3b0eb2c97414b473681717fa9f96864c0835e0bfbf130263398957543</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0006-291X(03)00773-3$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12745091$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kulartz, Monika</creatorcontrib><creatorcontrib>Kreitz, Sandra</creatorcontrib><creatorcontrib>Hiller, Ekkehard</creatorcontrib><creatorcontrib>Damoc, Elena-Catalina</creatorcontrib><creatorcontrib>Przybylski, Michael</creatorcontrib><creatorcontrib>Knippers, Rolf</creatorcontrib><title>Expression and phosphorylation of the replication regulator protein geminin</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>It has been described that the replication regulator protein geminin is rapidly degraded at the end of mitosis and newly expressed at the beginning of the next S phase in the metazoan cell cycle. We have performed experiments to investigate the synthesis of geminin in cycling human HeLa cells. The levels of geminin-mRNA vary only modestly during the cell cycle with a 2–3-fold higher mRNA level at the G1/S phase transition, whereas newly synthesized geminin can only be detected in post-G1 phases. Surprisingly, geminin, once synthesized, does not remain stable, but is turned over during S phase with a half-life of 3–4
h. We also show that geminin becomes phosphorylated as S phase proceeds and identify by MALDI mass spectrometry two specific major phosphorylation sites.</description><subject>Amino Acid Sequence</subject><subject>Cell cycle</subject><subject>Cell Cycle Proteins - biosynthesis</subject><subject>Cell Cycle Proteins - chemistry</subject><subject>Cell Cycle Proteins - genetics</subject><subject>Cell Cycle Proteins - metabolism</subject><subject>DNA Replication</subject><subject>Geminin</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Kinetics</subject><subject>Mass spectrometry</subject><subject>Molecular Sequence Data</subject><subject>Phosphorylation</subject><subject>Replication–regulation</subject><subject>RNA, Messenger - biosynthesis</subject><subject>S Phase</subject><subject>Transcription, Genetic</subject><subject>Turnover</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMlKxEAQhhtRnHF5BCUn0UO0ekkvJxEZFxQ8qOCtSXoqMy2ZJHYnom9vZkGPHoqCqq_qh4-QIwrnFKi8eAYAmTJD306BnwEoxVO-RcYUDKSMgtgm419kRPZifAegVEizS0aUKZGBoWPyMPlqA8bomzrJ62nSzps4VPiu8m45a8qkm2MSsK28W48Czvph24SkDU2Hvk5muPC1rw_ITplXEQ83fZ-83kxeru_Sx6fb--urx9SJTHcpU1IgmJIXgAVzRgkqCqG41FRRVeamNFJL4UDzDKEoi5JyYJJzo02mMsH3ycn675D_0WPs7MJHh1WV19j00SrOFNdM_wtSrZRmcglma9CFJsaApW2DX-Th21KwS912pdsuXVrgdqXb8uHueBPQFwuc_l1t_A7A5RrAwcenx2Cj81g7nPqArrPTxv8T8QOiMY7x</recordid><startdate>20030530</startdate><enddate>20030530</enddate><creator>Kulartz, Monika</creator><creator>Kreitz, Sandra</creator><creator>Hiller, Ekkehard</creator><creator>Damoc, Elena-Catalina</creator><creator>Przybylski, Michael</creator><creator>Knippers, Rolf</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>20030530</creationdate><title>Expression and phosphorylation of the replication regulator protein geminin</title><author>Kulartz, Monika ; Kreitz, Sandra ; Hiller, Ekkehard ; Damoc, Elena-Catalina ; Przybylski, Michael ; Knippers, Rolf</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c458t-2764e09f3b0eb2c97414b473681717fa9f96864c0835e0bfbf130263398957543</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Amino Acid Sequence</topic><topic>Cell cycle</topic><topic>Cell Cycle Proteins - biosynthesis</topic><topic>Cell Cycle Proteins - chemistry</topic><topic>Cell Cycle Proteins - genetics</topic><topic>Cell Cycle Proteins - metabolism</topic><topic>DNA Replication</topic><topic>Geminin</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Kinetics</topic><topic>Mass spectrometry</topic><topic>Molecular Sequence Data</topic><topic>Phosphorylation</topic><topic>Replication–regulation</topic><topic>RNA, Messenger - biosynthesis</topic><topic>S Phase</topic><topic>Transcription, Genetic</topic><topic>Turnover</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kulartz, Monika</creatorcontrib><creatorcontrib>Kreitz, Sandra</creatorcontrib><creatorcontrib>Hiller, Ekkehard</creatorcontrib><creatorcontrib>Damoc, Elena-Catalina</creatorcontrib><creatorcontrib>Przybylski, Michael</creatorcontrib><creatorcontrib>Knippers, Rolf</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kulartz, Monika</au><au>Kreitz, Sandra</au><au>Hiller, Ekkehard</au><au>Damoc, Elena-Catalina</au><au>Przybylski, Michael</au><au>Knippers, Rolf</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression and phosphorylation of the replication regulator protein geminin</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2003-05-30</date><risdate>2003</risdate><volume>305</volume><issue>2</issue><spage>412</spage><epage>420</epage><pages>412-420</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>It has been described that the replication regulator protein geminin is rapidly degraded at the end of mitosis and newly expressed at the beginning of the next S phase in the metazoan cell cycle. We have performed experiments to investigate the synthesis of geminin in cycling human HeLa cells. The levels of geminin-mRNA vary only modestly during the cell cycle with a 2–3-fold higher mRNA level at the G1/S phase transition, whereas newly synthesized geminin can only be detected in post-G1 phases. Surprisingly, geminin, once synthesized, does not remain stable, but is turned over during S phase with a half-life of 3–4
h. We also show that geminin becomes phosphorylated as S phase proceeds and identify by MALDI mass spectrometry two specific major phosphorylation sites.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>12745091</pmid><doi>10.1016/S0006-291X(03)00773-3</doi><tpages>9</tpages></addata></record> |
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| subjects | Amino Acid Sequence Cell cycle Cell Cycle Proteins - biosynthesis Cell Cycle Proteins - chemistry Cell Cycle Proteins - genetics Cell Cycle Proteins - metabolism DNA Replication Geminin HeLa Cells Humans Kinetics Mass spectrometry Molecular Sequence Data Phosphorylation Replication–regulation RNA, Messenger - biosynthesis S Phase Transcription, Genetic Turnover |
| title | Expression and phosphorylation of the replication regulator protein geminin |
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