Extension of filopodia by motor-dependent actin assembly
A variety of mechanisms have been proposed to explain the forward extension of cytoplasm in advancing cells and axonal growth cones, including actin polymerization and osmotic swelling. Based on our observations of the filopodia of cultured neuronal growth cones, we propose a mechanism involving mot...
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| Veröffentlicht in: | Cell motility and the cytoskeleton 1992, Vol.22 (3), p.160-169 |
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| container_title | Cell motility and the cytoskeleton |
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| creator | Sheetz, Michael P. Wayne, Denise B. Pearlman, Alan L. |
| description | A variety of mechanisms have been proposed to explain the forward extension of cytoplasm in advancing cells and axonal growth cones, including actin polymerization and osmotic swelling. Based on our observations of the filopodia of cultured neuronal growth cones, we propose a mechanism involving motor‐induced extension and retraction. We observed that filopodia (actin‐based protrusions 0.2–0.5 μ in diameter) extend and retract from growth cone lamellae at the same rate. Further, force is generated at the tips of filopodia which is sufficient to produce compressive buckling of the proximal portion of the filopodium. From our analysis of these movements we suggest that a motor protein powers both the extension and retraction of filopodia. © 1992 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/cm.970220303 |
| format | Article |
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From our analysis of these movements we suggest that a motor protein powers both the extension and retraction of filopodia. © 1992 Wiley‐Liss, Inc.</description><subject>actin polymerization</subject><subject>Actins - physiology</subject><subject>Animals</subject><subject>Cell Movement</subject><subject>Cytoskeleton - physiology</subject><subject>filament disassembly</subject><subject>growth cones</subject><subject>Models, Biological</subject><subject>myosin</subject><subject>Myosins - physiology</subject><subject>Neurites - physiology</subject><issn>0886-1544</issn><issn>1097-0169</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM9LwzAYhoMoc05vXoWePNn5JWma5ihjTmXzByoDLyFNE6i2zWw6XP97Kx3Tk6fv8D7vw8eL0CmGMQYgl7ocCw6EAAW6h4YYBA8Bx2IfDSFJ4hCzKDpER96_A2AccTZAAxwRGsdkiJLppjGVz10VOBvYvHArl-UqSNugdI2rw8ysTJWZqgmUbvIqUN6bMi3aY3RgVeHNyfaO0Ov19GVyE84fZreTq3moIxrTUESQpZxaAWkGYAhlllpQqUiiyDLOrMACG0sh0ywlcZxqxWz3nDJakUQpOkLnvXdVu8-18Y0sc69NUajKuLWXnBJOGRcdeNGDunbe18bKVZ2Xqm4lBvkzlNSl3A3V4Wdb7zotTfYL98t0Oevzr7ww7b8uOVn89YZ9L_eN2ex6qv6QMaecyeX9TC7elnePz_On7vtvnlaBzw</recordid><startdate>1992</startdate><enddate>1992</enddate><creator>Sheetz, Michael P.</creator><creator>Wayne, Denise B.</creator><creator>Pearlman, Alan L.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1992</creationdate><title>Extension of filopodia by motor-dependent actin assembly</title><author>Sheetz, Michael P. ; Wayne, Denise B. ; Pearlman, Alan L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4363-940db73f90bd00e235f3f0ab9844f575f9191ef30dc5b266bca5f423aeca28aa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>actin polymerization</topic><topic>Actins - physiology</topic><topic>Animals</topic><topic>Cell Movement</topic><topic>Cytoskeleton - physiology</topic><topic>filament disassembly</topic><topic>growth cones</topic><topic>Models, Biological</topic><topic>myosin</topic><topic>Myosins - physiology</topic><topic>Neurites - physiology</topic><toplevel>online_resources</toplevel><creatorcontrib>Sheetz, Michael P.</creatorcontrib><creatorcontrib>Wayne, Denise B.</creatorcontrib><creatorcontrib>Pearlman, Alan L.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cell motility and the cytoskeleton</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sheetz, Michael P.</au><au>Wayne, Denise B.</au><au>Pearlman, Alan L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Extension of filopodia by motor-dependent actin assembly</atitle><jtitle>Cell motility and the cytoskeleton</jtitle><addtitle>Cell Motil. 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| language | eng |
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| source | MEDLINE; Wiley Online Library All Journals |
| subjects | actin polymerization Actins - physiology Animals Cell Movement Cytoskeleton - physiology filament disassembly growth cones Models, Biological myosin Myosins - physiology Neurites - physiology |
| title | Extension of filopodia by motor-dependent actin assembly |
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