The Putative Collagen‐Binding Peptide P‐15 Promotes Fibroblast Attachment to Root Shavings but Not Hydroxyapatite

Background: Regenerative periodontal treatment aims to restore the attachment of the periodontal ligament and gingival collagen fibers to both the cementum of the root surface and alveolar bone. Fibroblasts are the predominant cells of the periodontal ligament and gingiva and have important roles in the function and regeneration of the tooth‐supporting apparatus. This study investigated whether a putative collagen‐based cellbinding peptide (P‐15) increases gingival fibroblast attachment to root shavings and bone replacement graft (BRG) materials. Methods: Gingival and dermal fibroblast attachment to root shavings and BRG materials, and cell proliferation on root shavings and sections were measured fluorometrically. Root shavings and root sections obtained from periodontally healthy teeth were treated with P‐15 at 2 concentrations (200 ng/g or 400 ng/g). Citric acid (CA)‐treated root materials were also compared to untreated root shavings and root sections that served as negative control groups. Results: Attachment of all cells to bone fragments (whether freeze‐dried or demineralized) was significantly greater than to hydroxyapatite (HA)‐based BRG materials. The addition of P‐15 to HA did not significantly increase gingival or dermal fibroblast attachment. At a concentration of 400 ng/g, P‐15 significantly increased gingival and dermal fibroblast attachment to root shavings as compared to untreated shavings. Bone fragments, HA‐based BRG materials, and untreated root shavings inhibited gingival fibroblast proliferation. Treatment of root sections with P‐15 did not have any effect on gingival fibroblast proliferation. Conclusions: P‐15 is a potential alternative to CA for promoting fibroblast attachment to root surfaces. However, P‐15 did not enhance fibroblast proliferation on root sections. J Periodontol 2003;74:458‐467.