Direct antiviral effect of cycloferon (10-carboxymethyl-9-acridanone) against adenovirus type 6 in vitro
Adenoviruses represent a broad group of human pathogens that currently have no specific and safe drugs for treatment. We demonstrated direct (non IFN-mediated) antiviral activity of cycloferon (10-carboxymethyl-9-acridanone, CMA), a potent interferon inducer, against adenovirus type 6 (Ad6) in Hep-2...
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| Veröffentlicht in: | Antiviral research 2003-04, Vol.58 (2), p.131-137 |
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| container_title | Antiviral research |
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| creator | Zarubaev, V.V. Slita, A.V. Krivitskaya, V.Z. Sirotkin, A.K. Kovalenko, A.L. Chatterjee, N.K. |
| description | Adenoviruses represent a broad group of human pathogens that currently have no specific and safe drugs for treatment. We demonstrated direct (non IFN-mediated) antiviral activity of cycloferon (10-carboxymethyl-9-acridanone, CMA), a potent interferon inducer, against adenovirus type 6 (Ad6) in Hep-2 cells. Virus production and details of morphogenesis were studied by ELISA with antibodies to the Ad6 hexon protein, and transmission electron microscopy, respectively. Immunoenzyme assay revealed that CMA does not inhibit viral protein synthesis but instead strongly reduces the ability of the virus to generate infectious progeny virus in a dose dependent manner. Ultrastructural study shows that CMA alters the structure of intranuclear virus-specific inclusions. We suggest that CMA suppresses the late stages of viral cycle in the infected cell. |
| doi_str_mv | 10.1016/S0166-3542(02)00193-6 |
| format | Article |
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We demonstrated direct (non IFN-mediated) antiviral activity of cycloferon (10-carboxymethyl-9-acridanone, CMA), a potent interferon inducer, against adenovirus type 6 (Ad6) in Hep-2 cells. Virus production and details of morphogenesis were studied by ELISA with antibodies to the Ad6 hexon protein, and transmission electron microscopy, respectively. Immunoenzyme assay revealed that CMA does not inhibit viral protein synthesis but instead strongly reduces the ability of the virus to generate infectious progeny virus in a dose dependent manner. Ultrastructural study shows that CMA alters the structure of intranuclear virus-specific inclusions. 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We demonstrated direct (non IFN-mediated) antiviral activity of cycloferon (10-carboxymethyl-9-acridanone, CMA), a potent interferon inducer, against adenovirus type 6 (Ad6) in Hep-2 cells. Virus production and details of morphogenesis were studied by ELISA with antibodies to the Ad6 hexon protein, and transmission electron microscopy, respectively. Immunoenzyme assay revealed that CMA does not inhibit viral protein synthesis but instead strongly reduces the ability of the virus to generate infectious progeny virus in a dose dependent manner. Ultrastructural study shows that CMA alters the structure of intranuclear virus-specific inclusions. We suggest that CMA suppresses the late stages of viral cycle in the infected cell.</description><subject>Acridone</subject><subject>Adenovirus</subject><subject>Adenoviruses, Human - drug effects</subject><subject>Adenoviruses, Human - physiology</subject><subject>Adenoviruses, Human - ultrastructure</subject><subject>Antiviral</subject><subject>Antiviral Agents - pharmacology</subject><subject>Cell Nucleus - drug effects</subject><subject>Cell Nucleus - ultrastructure</subject><subject>Cell Nucleus - virology</subject><subject>Dose-Response Relationship, Drug</subject><subject>Electron microscopy</subject><subject>Humans</subject><subject>Interferon Inducers - pharmacology</subject><subject>Microscopy, Electron</subject><subject>Proviruses - drug effects</subject><subject>Proviruses - physiology</subject><subject>Tumor Cells, Cultured</subject><subject>Virus Replication - drug effects</subject><issn>0166-3542</issn><issn>1872-9096</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUGLFDEQhYMo7rj6E5Q-ye4hWkm6k85JltVVYcGDeg6ZpOJGepIx6Rnsf29mZ1iPC6FSFF-9gvcIec3gHQMm339vRVIx9PwC-CUA04LKJ2TFRsWpBi2fktUDckZe1PobAKTS43Nyxrjq-aDEitx9jAXd3Nk0x30sduowhMMgh84tbsoBS07dBQPqbFnnv8sG57tloppaV6K3KSe87OwvG1NtMh5Tbjq72s3LFjvZxdTt41zyS_Is2Kniq9N_Tn7efPpx_YXefvv89frqljqh-UyFVIPtAa3VMATnQAvF-p4HjTqMowdY-0EBeG6VQ6uV8qHXzAfWGhCjOCdvj7rbkv_ssM5mE6vDabIJ864aJbjsNWePgs1IJaDXDRyOoCu51oLBbEvc2LIYBuaQhbnPwhyMNtDeIQsj296b04HdeoP-_9bJ_AZ8OALY_NhHLKa6iMmhv8_E-BwfOfEPBHeZBA</recordid><startdate>20030401</startdate><enddate>20030401</enddate><creator>Zarubaev, V.V.</creator><creator>Slita, A.V.</creator><creator>Krivitskaya, V.Z.</creator><creator>Sirotkin, A.K.</creator><creator>Kovalenko, A.L.</creator><creator>Chatterjee, N.K.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20030401</creationdate><title>Direct antiviral effect of cycloferon (10-carboxymethyl-9-acridanone) against adenovirus type 6 in vitro</title><author>Zarubaev, V.V. ; 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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Acridone Adenovirus Adenoviruses, Human - drug effects Adenoviruses, Human - physiology Adenoviruses, Human - ultrastructure Antiviral Antiviral Agents - pharmacology Cell Nucleus - drug effects Cell Nucleus - ultrastructure Cell Nucleus - virology Dose-Response Relationship, Drug Electron microscopy Humans Interferon Inducers - pharmacology Microscopy, Electron Proviruses - drug effects Proviruses - physiology Tumor Cells, Cultured Virus Replication - drug effects |
| title | Direct antiviral effect of cycloferon (10-carboxymethyl-9-acridanone) against adenovirus type 6 in vitro |
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