cDNA microarray analysis of genes associated with ERBB2 (HER2/neu) overexpression in human mammary luminal epithelial cells
To investigate changes in gene expression associated with ERBB2 , expression profiling of immortalized human mammary luminal epithelial cells and variants expressing a moderate and high level of ERBB2 has been carried out using cDNA microarrays corresponding to approximately 6000 unique genes/ESTs....
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| Veröffentlicht in: | Oncogene 2003-05, Vol.22 (17), p.2680-2688 |
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| creator | Mackay, Alan Jones, Chris Dexter, Tim Silva, Ricardo L A Bulmer, Karen Jones, Allison Simpson, Peter Harris, Robert A Jat, Parmjit S Neville, A Munro Reis, Luiz F L Lakhani, Sunil R O'Hare, Michael J |
| description | To investigate changes in gene expression associated with
ERBB2
, expression profiling of immortalized human mammary luminal epithelial cells and variants expressing a moderate and high level of
ERBB2
has been carried out using cDNA microarrays corresponding to approximately 6000 unique genes/ESTs. A total of 61 significantly up- or downregulated (2.0-fold) genes were identified and further validated by RT–PCR analysis as well as microarray comparisons with a spontaneously
ERBB2
- overexpressing breast cancer cell line and
ERBB2
-positive primary breast tumors. The expression and clinical relevance of proteins predicted to be associated with
ERBB2
overexpression in breast cancers were analysed together with their clinical relevance by antibody screening using a tissue array. Differentially regulated genes include those involved in cell–matrix interactions including proline 4-hydroxylase (
P4HA2
), galectin 1 (
LGALS1
) and galectin 3 (
LGALS3
), fibronectin 1 (
FN1
) and p-cadherin (
CDH3
), and cell proliferation (
CRIP1
,
IGFBP3
) and transformation (
S100P
,
S100A4
). A number of genes associated with
MYC
signalling were also differentially expressed, including
NDRG1
,
USF2
and the epithelial membrane proteins 1 and 3 (
EMP1
,
EMP3
). These data represent profiles of the transcriptional changes associated with
ERBB2
-related pathways in the breast, and identify novel and potentially useful targets for prognosis and therapy. |
| doi_str_mv | 10.1038/sj.onc.1206349 |
| format | Article |
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ERBB2
, expression profiling of immortalized human mammary luminal epithelial cells and variants expressing a moderate and high level of
ERBB2
has been carried out using cDNA microarrays corresponding to approximately 6000 unique genes/ESTs. A total of 61 significantly up- or downregulated (2.0-fold) genes were identified and further validated by RT–PCR analysis as well as microarray comparisons with a spontaneously
ERBB2
- overexpressing breast cancer cell line and
ERBB2
-positive primary breast tumors. The expression and clinical relevance of proteins predicted to be associated with
ERBB2
overexpression in breast cancers were analysed together with their clinical relevance by antibody screening using a tissue array. Differentially regulated genes include those involved in cell–matrix interactions including proline 4-hydroxylase (
P4HA2
), galectin 1 (
LGALS1
) and galectin 3 (
LGALS3
), fibronectin 1 (
FN1
) and p-cadherin (
CDH3
), and cell proliferation (
CRIP1
,
IGFBP3
) and transformation (
S100P
,
S100A4
). A number of genes associated with
MYC
signalling were also differentially expressed, including
NDRG1
,
USF2
and the epithelial membrane proteins 1 and 3 (
EMP1
,
EMP3
). These data represent profiles of the transcriptional changes associated with
ERBB2
-related pathways in the breast, and identify novel and potentially useful targets for prognosis and therapy.</description><identifier>ISSN: 0950-9232</identifier><identifier>EISSN: 1476-5594</identifier><identifier>DOI: 10.1038/sj.onc.1206349</identifier><identifier>PMID: 12730682</identifier><identifier>CODEN: ONCNES</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Apoptosis ; Biological and medical sciences ; Breast - metabolism ; Breast cancer ; Breast Neoplasms - metabolism ; Cadherins ; Carcinoma - metabolism ; Cell Biology ; Cell proliferation ; Classical genetics, quantitative genetics, hybrids ; DNA microarrays ; Epithelial cells ; Epithelium - metabolism ; ErbB-2 protein ; Female ; Fibronectin ; Fundamental and applied biological sciences. Psychology ; Gene expression ; Gene Expression Profiling ; Genetics of eukaryotes. Biological and molecular evolution ; Human ; Human Genetics ; Humans ; Hydroxylase ; In Vitro Techniques ; Insulin-like growth factor-binding protein 3 ; Internal Medicine ; Mammary gland ; Medicine ; Medicine & Public Health ; Membrane proteins ; Myc protein ; Oligonucleotide Array Sequence Analysis ; oncogenomics ; Oncology ; Proline ; Receptor, ErbB-2 - biosynthesis ; Receptor, ErbB-2 - genetics ; Reverse Transcriptase Polymerase Chain Reaction ; S100A4 protein ; Signal transduction ; Transcription ; Tumor Cells, Cultured ; Tumors</subject><ispartof>Oncogene, 2003-05, Vol.22 (17), p.2680-2688</ispartof><rights>Springer Nature Limited 2003</rights><rights>2003 INIST-CNRS</rights><rights>COPYRIGHT 2003 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group May 1, 2003</rights><rights>Nature Publishing Group 2003.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c554t-6735ccc4002e90e33179f9139f2217cc043e0baf6b9b85ee10624766763cacd93</citedby><cites>FETCH-LOGICAL-c554t-6735ccc4002e90e33179f9139f2217cc043e0baf6b9b85ee10624766763cacd93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14745861$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12730682$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mackay, Alan</creatorcontrib><creatorcontrib>Jones, Chris</creatorcontrib><creatorcontrib>Dexter, Tim</creatorcontrib><creatorcontrib>Silva, Ricardo L A</creatorcontrib><creatorcontrib>Bulmer, Karen</creatorcontrib><creatorcontrib>Jones, Allison</creatorcontrib><creatorcontrib>Simpson, Peter</creatorcontrib><creatorcontrib>Harris, Robert A</creatorcontrib><creatorcontrib>Jat, Parmjit S</creatorcontrib><creatorcontrib>Neville, A Munro</creatorcontrib><creatorcontrib>Reis, Luiz F L</creatorcontrib><creatorcontrib>Lakhani, Sunil R</creatorcontrib><creatorcontrib>O'Hare, Michael J</creatorcontrib><title>cDNA microarray analysis of genes associated with ERBB2 (HER2/neu) overexpression in human mammary luminal epithelial cells</title><title>Oncogene</title><addtitle>Oncogene</addtitle><addtitle>Oncogene</addtitle><description>To investigate changes in gene expression associated with
ERBB2
, expression profiling of immortalized human mammary luminal epithelial cells and variants expressing a moderate and high level of
ERBB2
has been carried out using cDNA microarrays corresponding to approximately 6000 unique genes/ESTs. A total of 61 significantly up- or downregulated (2.0-fold) genes were identified and further validated by RT–PCR analysis as well as microarray comparisons with a spontaneously
ERBB2
- overexpressing breast cancer cell line and
ERBB2
-positive primary breast tumors. The expression and clinical relevance of proteins predicted to be associated with
ERBB2
overexpression in breast cancers were analysed together with their clinical relevance by antibody screening using a tissue array. Differentially regulated genes include those involved in cell–matrix interactions including proline 4-hydroxylase (
P4HA2
), galectin 1 (
LGALS1
) and galectin 3 (
LGALS3
), fibronectin 1 (
FN1
) and p-cadherin (
CDH3
), and cell proliferation (
CRIP1
,
IGFBP3
) and transformation (
S100P
,
S100A4
). A number of genes associated with
MYC
signalling were also differentially expressed, including
NDRG1
,
USF2
and the epithelial membrane proteins 1 and 3 (
EMP1
,
EMP3
). These data represent profiles of the transcriptional changes associated with
ERBB2
-related pathways in the breast, and identify novel and potentially useful targets for prognosis and therapy.</description><subject>Apoptosis</subject><subject>Biological and medical sciences</subject><subject>Breast - metabolism</subject><subject>Breast cancer</subject><subject>Breast Neoplasms - metabolism</subject><subject>Cadherins</subject><subject>Carcinoma - metabolism</subject><subject>Cell Biology</subject><subject>Cell proliferation</subject><subject>Classical genetics, quantitative genetics, hybrids</subject><subject>DNA microarrays</subject><subject>Epithelial cells</subject><subject>Epithelium - metabolism</subject><subject>ErbB-2 protein</subject><subject>Female</subject><subject>Fibronectin</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>Human</subject><subject>Human Genetics</subject><subject>Humans</subject><subject>Hydroxylase</subject><subject>In Vitro Techniques</subject><subject>Insulin-like growth factor-binding protein 3</subject><subject>Internal Medicine</subject><subject>Mammary gland</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Membrane proteins</subject><subject>Myc protein</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>oncogenomics</subject><subject>Oncology</subject><subject>Proline</subject><subject>Receptor, ErbB-2 - biosynthesis</subject><subject>Receptor, ErbB-2 - genetics</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>S100A4 protein</subject><subject>Signal transduction</subject><subject>Transcription</subject><subject>Tumor Cells, Cultured</subject><subject>Tumors</subject><issn>0950-9232</issn><issn>1476-5594</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkt1rUzEYxg-iuG5666UERdGLtvnOyWU3qxOGwtDrkKbv6VLOyeny9uiK_7wpKxRkQ3KRkPzerzxPVb1idMKoqKe4nvQpTBinWkj7pBoxafRYKSufViNqFR1bLvhJdYq4ppQaS_nz6oRxI6iu-aj6Ez59m5Euhtz7nP2O-OTbHUYkfUNWkACJR-xD9FtYkt9xe0Pm1-fnnHy4nF_zaYLhI-l_QYa7TQbE2CcSE7kZOp9I57vO5x1phy6WrAQ2JRzaWI4B2hZfVM8a3yK8POxn1c_P8x8Xl-Or71--XsyuxkEpuR1rI1QIQVLKwVIQghnbWCZswzkzIVApgC58oxd2USsARjUvn6CNFsGHpRVn1fv7vJvc3w6AW9dF3HfgE_QDOiO4ZpKa_4KsNkZIqQv49h9w3Q-5zIiOa8kEr5lQhXrzKFUEUIwxdUy18i24mJp-m33Y13UzVlvO6pqKQk0eoMpaQtGuT9DEcv9QQBEWMUPjNjnu1XCMur1zHK5dcY47OKcEvD40Oyw6WB7xg1UK8O4AeAy-bbJPIeKRk0aqWrPCTe85LE9pBfk49SOl_wLA1tiu</recordid><startdate>20030501</startdate><enddate>20030501</enddate><creator>Mackay, Alan</creator><creator>Jones, Chris</creator><creator>Dexter, Tim</creator><creator>Silva, Ricardo L A</creator><creator>Bulmer, Karen</creator><creator>Jones, Allison</creator><creator>Simpson, Peter</creator><creator>Harris, Robert A</creator><creator>Jat, Parmjit S</creator><creator>Neville, A Munro</creator><creator>Reis, Luiz F L</creator><creator>Lakhani, Sunil R</creator><creator>O'Hare, Michael J</creator><general>Nature Publishing Group UK</general><general>Nature Publishing</general><general>Nature Publishing Group</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20030501</creationdate><title>cDNA microarray analysis of genes associated with ERBB2 (HER2/neu) overexpression in human mammary luminal epithelial cells</title><author>Mackay, Alan ; Jones, Chris ; Dexter, Tim ; Silva, Ricardo L A ; Bulmer, Karen ; Jones, Allison ; Simpson, Peter ; Harris, Robert A ; Jat, Parmjit S ; Neville, A Munro ; Reis, Luiz F L ; Lakhani, Sunil R ; O'Hare, Michael J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c554t-6735ccc4002e90e33179f9139f2217cc043e0baf6b9b85ee10624766763cacd93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Apoptosis</topic><topic>Biological and medical sciences</topic><topic>Breast - metabolism</topic><topic>Breast cancer</topic><topic>Breast Neoplasms - metabolism</topic><topic>Cadherins</topic><topic>Carcinoma - metabolism</topic><topic>Cell Biology</topic><topic>Cell proliferation</topic><topic>Classical genetics, quantitative genetics, hybrids</topic><topic>DNA microarrays</topic><topic>Epithelial cells</topic><topic>Epithelium - metabolism</topic><topic>ErbB-2 protein</topic><topic>Female</topic><topic>Fibronectin</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression</topic><topic>Gene Expression Profiling</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>Human</topic><topic>Human Genetics</topic><topic>Humans</topic><topic>Hydroxylase</topic><topic>In Vitro Techniques</topic><topic>Insulin-like growth factor-binding protein 3</topic><topic>Internal Medicine</topic><topic>Mammary gland</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Membrane proteins</topic><topic>Myc protein</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>oncogenomics</topic><topic>Oncology</topic><topic>Proline</topic><topic>Receptor, ErbB-2 - biosynthesis</topic><topic>Receptor, ErbB-2 - genetics</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>S100A4 protein</topic><topic>Signal transduction</topic><topic>Transcription</topic><topic>Tumor Cells, Cultured</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mackay, Alan</creatorcontrib><creatorcontrib>Jones, Chris</creatorcontrib><creatorcontrib>Dexter, Tim</creatorcontrib><creatorcontrib>Silva, Ricardo L A</creatorcontrib><creatorcontrib>Bulmer, Karen</creatorcontrib><creatorcontrib>Jones, Allison</creatorcontrib><creatorcontrib>Simpson, Peter</creatorcontrib><creatorcontrib>Harris, Robert A</creatorcontrib><creatorcontrib>Jat, Parmjit S</creatorcontrib><creatorcontrib>Neville, A Munro</creatorcontrib><creatorcontrib>Reis, Luiz F L</creatorcontrib><creatorcontrib>Lakhani, Sunil R</creatorcontrib><creatorcontrib>O'Hare, Michael J</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Oncogene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mackay, Alan</au><au>Jones, Chris</au><au>Dexter, Tim</au><au>Silva, Ricardo L A</au><au>Bulmer, Karen</au><au>Jones, Allison</au><au>Simpson, Peter</au><au>Harris, Robert A</au><au>Jat, Parmjit S</au><au>Neville, A Munro</au><au>Reis, Luiz F L</au><au>Lakhani, Sunil R</au><au>O'Hare, Michael J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>cDNA microarray analysis of genes associated with ERBB2 (HER2/neu) overexpression in human mammary luminal epithelial cells</atitle><jtitle>Oncogene</jtitle><stitle>Oncogene</stitle><addtitle>Oncogene</addtitle><date>2003-05-01</date><risdate>2003</risdate><volume>22</volume><issue>17</issue><spage>2680</spage><epage>2688</epage><pages>2680-2688</pages><issn>0950-9232</issn><eissn>1476-5594</eissn><coden>ONCNES</coden><abstract>To investigate changes in gene expression associated with
ERBB2
, expression profiling of immortalized human mammary luminal epithelial cells and variants expressing a moderate and high level of
ERBB2
has been carried out using cDNA microarrays corresponding to approximately 6000 unique genes/ESTs. A total of 61 significantly up- or downregulated (2.0-fold) genes were identified and further validated by RT–PCR analysis as well as microarray comparisons with a spontaneously
ERBB2
- overexpressing breast cancer cell line and
ERBB2
-positive primary breast tumors. The expression and clinical relevance of proteins predicted to be associated with
ERBB2
overexpression in breast cancers were analysed together with their clinical relevance by antibody screening using a tissue array. Differentially regulated genes include those involved in cell–matrix interactions including proline 4-hydroxylase (
P4HA2
), galectin 1 (
LGALS1
) and galectin 3 (
LGALS3
), fibronectin 1 (
FN1
) and p-cadherin (
CDH3
), and cell proliferation (
CRIP1
,
IGFBP3
) and transformation (
S100P
,
S100A4
). A number of genes associated with
MYC
signalling were also differentially expressed, including
NDRG1
,
USF2
and the epithelial membrane proteins 1 and 3 (
EMP1
,
EMP3
). These data represent profiles of the transcriptional changes associated with
ERBB2
-related pathways in the breast, and identify novel and potentially useful targets for prognosis and therapy.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>12730682</pmid><doi>10.1038/sj.onc.1206349</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
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| ispartof | Oncogene, 2003-05, Vol.22 (17), p.2680-2688 |
| issn | 0950-9232 1476-5594 |
| language | eng |
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| source | MEDLINE; Nature; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Apoptosis Biological and medical sciences Breast - metabolism Breast cancer Breast Neoplasms - metabolism Cadherins Carcinoma - metabolism Cell Biology Cell proliferation Classical genetics, quantitative genetics, hybrids DNA microarrays Epithelial cells Epithelium - metabolism ErbB-2 protein Female Fibronectin Fundamental and applied biological sciences. Psychology Gene expression Gene Expression Profiling Genetics of eukaryotes. Biological and molecular evolution Human Human Genetics Humans Hydroxylase In Vitro Techniques Insulin-like growth factor-binding protein 3 Internal Medicine Mammary gland Medicine Medicine & Public Health Membrane proteins Myc protein Oligonucleotide Array Sequence Analysis oncogenomics Oncology Proline Receptor, ErbB-2 - biosynthesis Receptor, ErbB-2 - genetics Reverse Transcriptase Polymerase Chain Reaction S100A4 protein Signal transduction Transcription Tumor Cells, Cultured Tumors |
| title | cDNA microarray analysis of genes associated with ERBB2 (HER2/neu) overexpression in human mammary luminal epithelial cells |
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