The complete nucleotide sequence of pepper mottle virus genomic RNA: Comparison of the encoded polyprotein with those of other sequenced potyviruses
The complete nucleotide sequence of a pepper mottle virus isolate from California (PepMoV C) has been determined from cloned viral cDNAs. The PepMoV C genomic RNA is 9640 nucleotides excluding the poly(A) tail and contains a long open reading frame starting at nucleotide 168 and potentially encoding...
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| Veröffentlicht in: | Virology (New York, N.Y.) N.Y.), 1992-11, Vol.191 (1), p.19-30 |
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| creator | Vance, Vicki Bowman Moore, Delores Turpen, Thomas H. Bracker, Allan Hollowell, Victoria C. |
| description | The complete nucleotide sequence of a pepper mottle virus isolate from California (PepMoV C) has been determined from cloned viral cDNAs. The PepMoV C genomic RNA is 9640 nucleotides excluding the poly(A) tail and contains a long open reading frame starting at nucleotide 168 and potentially encoding a polyprotein of 3068 amino acids. Comparison of the PepMoV C presumptive polyprotein with those of other sequenced members of the potyvirus group, including tobacco etch virus (TEV), tobacco vein mottling virus (TVMV), plum pox virus (PPV), and potato virus Y (PVY), allowed localization of putative protein cleavage sites. A similar analysis was used to determine the position of conserved viral protein-coding regions along the viral genomic RNA. These analyses confirm previous work indicating that genome organization is conserved among members of the genus
Potyvirus. The localization of one PepMoV C gene product, the nuclear inclusion body protein a (Nla protein), was analyzed by expressing PepMoV cDNA deletion clones in bacteria and assaying for appearance of mature-sized coat protein, a cleavage product of the Nla protease. Comparative sequence analyses of the putative PepMoV polyprotein with those of TEV, TVMV, PPV, and PVY served to identify regions of the potyviral polyproteins which have diverged within the genus, as well as highly conserved protein features which may play an important functional role in the potyviral life cycle. |
| doi_str_mv | 10.1016/0042-6822(92)90162-I |
| format | Article |
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Potyvirus. The localization of one PepMoV C gene product, the nuclear inclusion body protein a (Nla protein), was analyzed by expressing PepMoV cDNA deletion clones in bacteria and assaying for appearance of mature-sized coat protein, a cleavage product of the Nla protease. Comparative sequence analyses of the putative PepMoV polyprotein with those of TEV, TVMV, PPV, and PVY served to identify regions of the potyviral polyproteins which have diverged within the genus, as well as highly conserved protein features which may play an important functional role in the potyviral life cycle.</description><identifier>ISSN: 0042-6822</identifier><identifier>EISSN: 1096-0341</identifier><identifier>DOI: 10.1016/0042-6822(92)90162-I</identifier><identifier>PMID: 1413501</identifier><identifier>CODEN: VIRLAX</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Amino Acid Sequence ; ARN ; Base Sequence ; Biological and medical sciences ; Endopeptidases - metabolism ; Fundamental and applied biological sciences. Psychology ; Genetics ; GENOMAS ; GENOME ; Genome, Viral ; Microbiology ; Molecular Sequence Data ; NUCLEOTIDE ; NUCLEOTIDOS ; Open Reading Frames ; Plant Viruses - genetics ; plum pox virus ; POTYVIRUS ; PROTEINAS ; PROTEINE ; RNA, Viral - genetics ; Sequence Homology, Amino Acid ; tobacco etch virus ; tobacco vein mottling virus ; Viral Proteins - genetics ; Viral Proteins - metabolism ; Virology ; VIRUS DE LAS PLANTAS ; VIRUS DES VEGETAUX</subject><ispartof>Virology (New York, N.Y.), 1992-11, Vol.191 (1), p.19-30</ispartof><rights>1992</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c436t-8fd0602d0d67718db71d7c197cccf631cb06a601243d4ebc93a82dd590bad5613</citedby><cites>FETCH-LOGICAL-c436t-8fd0602d0d67718db71d7c197cccf631cb06a601243d4ebc93a82dd590bad5613</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0042-6822(92)90162-I$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4447313$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1413501$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vance, Vicki Bowman</creatorcontrib><creatorcontrib>Moore, Delores</creatorcontrib><creatorcontrib>Turpen, Thomas H.</creatorcontrib><creatorcontrib>Bracker, Allan</creatorcontrib><creatorcontrib>Hollowell, Victoria C.</creatorcontrib><title>The complete nucleotide sequence of pepper mottle virus genomic RNA: Comparison of the encoded polyprotein with those of other sequenced potyviruses</title><title>Virology (New York, N.Y.)</title><addtitle>Virology</addtitle><description>The complete nucleotide sequence of a pepper mottle virus isolate from California (PepMoV C) has been determined from cloned viral cDNAs. The PepMoV C genomic RNA is 9640 nucleotides excluding the poly(A) tail and contains a long open reading frame starting at nucleotide 168 and potentially encoding a polyprotein of 3068 amino acids. Comparison of the PepMoV C presumptive polyprotein with those of other sequenced members of the potyvirus group, including tobacco etch virus (TEV), tobacco vein mottling virus (TVMV), plum pox virus (PPV), and potato virus Y (PVY), allowed localization of putative protein cleavage sites. A similar analysis was used to determine the position of conserved viral protein-coding regions along the viral genomic RNA. These analyses confirm previous work indicating that genome organization is conserved among members of the genus
Potyvirus. The localization of one PepMoV C gene product, the nuclear inclusion body protein a (Nla protein), was analyzed by expressing PepMoV cDNA deletion clones in bacteria and assaying for appearance of mature-sized coat protein, a cleavage product of the Nla protease. Comparative sequence analyses of the putative PepMoV polyprotein with those of TEV, TVMV, PPV, and PVY served to identify regions of the potyviral polyproteins which have diverged within the genus, as well as highly conserved protein features which may play an important functional role in the potyviral life cycle.</description><subject>Amino Acid Sequence</subject><subject>ARN</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Endopeptidases - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetics</subject><subject>GENOMAS</subject><subject>GENOME</subject><subject>Genome, Viral</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>NUCLEOTIDE</subject><subject>NUCLEOTIDOS</subject><subject>Open Reading Frames</subject><subject>Plant Viruses - genetics</subject><subject>plum pox virus</subject><subject>POTYVIRUS</subject><subject>PROTEINAS</subject><subject>PROTEINE</subject><subject>RNA, Viral - genetics</subject><subject>Sequence Homology, Amino Acid</subject><subject>tobacco etch virus</subject><subject>tobacco vein mottling virus</subject><subject>Viral Proteins - genetics</subject><subject>Viral Proteins - metabolism</subject><subject>Virology</subject><subject>VIRUS DE LAS PLANTAS</subject><subject>VIRUS DES VEGETAUX</subject><issn>0042-6822</issn><issn>1096-0341</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV2L1DAUhoso67j6B0QhFyJ6Uc1X08YLYRn8GFgUdPc6pMnpbqRtapKuzP_wB5tOh_FOIRCS9znvOZy3KJ4R_IZgIt5izGkpGkpfSfpa5h9a7u4VG4KlKDHj5H6xOSEPi0cx_sD5Xdf4rDgjnLAKk03x--oWkPHD1EMCNM6mB5-cBRTh5wyjAeQ7NME0QUCDT6kHdOfCHNENjH5wBn37cvEObbOBDi76ccFTtsyl3oJFk-_3U_AJ3Ih-uXSbRR8Ppj5j4dRmIdP-YA3xcfGg032EJ8f7vLj--OFq-7m8_Pppt724LA1nIpVNZ7HA1GIr6po0tq2JrQ2RtTGmE4yYFgstMKGcWQ6tkUw31NpK4lbbShB2XrxcffOEeYyY1OCigb7XI_g5qprRilJW_xckgklSNTiDfAVN8DEG6NQU3KDDXhGsltTUEolaIlEynyU1tctlz4_-czuA_Vu0xpT1F0ddR6P7LujRuHjCOOc1IyxjT1es017pmxyIuv4uGW0q2WTx_SpC3uidg6CicYfVuwAmKevdv4f8A1fpvj4</recordid><startdate>19921101</startdate><enddate>19921101</enddate><creator>Vance, Vicki Bowman</creator><creator>Moore, Delores</creator><creator>Turpen, Thomas H.</creator><creator>Bracker, Allan</creator><creator>Hollowell, Victoria C.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19921101</creationdate><title>The complete nucleotide sequence of pepper mottle virus genomic RNA: Comparison of the encoded polyprotein with those of other sequenced potyviruses</title><author>Vance, Vicki Bowman ; Moore, Delores ; Turpen, Thomas H. ; Bracker, Allan ; Hollowell, Victoria C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c436t-8fd0602d0d67718db71d7c197cccf631cb06a601243d4ebc93a82dd590bad5613</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Amino Acid Sequence</topic><topic>ARN</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Endopeptidases - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetics</topic><topic>GENOMAS</topic><topic>GENOME</topic><topic>Genome, Viral</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>NUCLEOTIDE</topic><topic>NUCLEOTIDOS</topic><topic>Open Reading Frames</topic><topic>Plant Viruses - genetics</topic><topic>plum pox virus</topic><topic>POTYVIRUS</topic><topic>PROTEINAS</topic><topic>PROTEINE</topic><topic>RNA, Viral - genetics</topic><topic>Sequence Homology, Amino Acid</topic><topic>tobacco etch virus</topic><topic>tobacco vein mottling virus</topic><topic>Viral Proteins - genetics</topic><topic>Viral Proteins - metabolism</topic><topic>Virology</topic><topic>VIRUS DE LAS PLANTAS</topic><topic>VIRUS DES VEGETAUX</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vance, Vicki Bowman</creatorcontrib><creatorcontrib>Moore, Delores</creatorcontrib><creatorcontrib>Turpen, Thomas H.</creatorcontrib><creatorcontrib>Bracker, Allan</creatorcontrib><creatorcontrib>Hollowell, Victoria C.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Virology (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vance, Vicki Bowman</au><au>Moore, Delores</au><au>Turpen, Thomas H.</au><au>Bracker, Allan</au><au>Hollowell, Victoria C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The complete nucleotide sequence of pepper mottle virus genomic RNA: Comparison of the encoded polyprotein with those of other sequenced potyviruses</atitle><jtitle>Virology (New York, N.Y.)</jtitle><addtitle>Virology</addtitle><date>1992-11-01</date><risdate>1992</risdate><volume>191</volume><issue>1</issue><spage>19</spage><epage>30</epage><pages>19-30</pages><issn>0042-6822</issn><eissn>1096-0341</eissn><coden>VIRLAX</coden><abstract>The complete nucleotide sequence of a pepper mottle virus isolate from California (PepMoV C) has been determined from cloned viral cDNAs. The PepMoV C genomic RNA is 9640 nucleotides excluding the poly(A) tail and contains a long open reading frame starting at nucleotide 168 and potentially encoding a polyprotein of 3068 amino acids. Comparison of the PepMoV C presumptive polyprotein with those of other sequenced members of the potyvirus group, including tobacco etch virus (TEV), tobacco vein mottling virus (TVMV), plum pox virus (PPV), and potato virus Y (PVY), allowed localization of putative protein cleavage sites. A similar analysis was used to determine the position of conserved viral protein-coding regions along the viral genomic RNA. These analyses confirm previous work indicating that genome organization is conserved among members of the genus
Potyvirus. The localization of one PepMoV C gene product, the nuclear inclusion body protein a (Nla protein), was analyzed by expressing PepMoV cDNA deletion clones in bacteria and assaying for appearance of mature-sized coat protein, a cleavage product of the Nla protease. Comparative sequence analyses of the putative PepMoV polyprotein with those of TEV, TVMV, PPV, and PVY served to identify regions of the potyviral polyproteins which have diverged within the genus, as well as highly conserved protein features which may play an important functional role in the potyviral life cycle.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>1413501</pmid><doi>10.1016/0042-6822(92)90162-I</doi><tpages>12</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0042-6822 |
| ispartof | Virology (New York, N.Y.), 1992-11, Vol.191 (1), p.19-30 |
| issn | 0042-6822 1096-0341 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73252237 |
| source | MEDLINE; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Amino Acid Sequence ARN Base Sequence Biological and medical sciences Endopeptidases - metabolism Fundamental and applied biological sciences. Psychology Genetics GENOMAS GENOME Genome, Viral Microbiology Molecular Sequence Data NUCLEOTIDE NUCLEOTIDOS Open Reading Frames Plant Viruses - genetics plum pox virus POTYVIRUS PROTEINAS PROTEINE RNA, Viral - genetics Sequence Homology, Amino Acid tobacco etch virus tobacco vein mottling virus Viral Proteins - genetics Viral Proteins - metabolism Virology VIRUS DE LAS PLANTAS VIRUS DES VEGETAUX |
| title | The complete nucleotide sequence of pepper mottle virus genomic RNA: Comparison of the encoded polyprotein with those of other sequenced potyviruses |
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