Determination of 4-n-nonylphenol in water by enzyme immunoassay
A range of monoclonal antibody-based competitive immunoassays in the format of microtitre plate ELISA and dipstick tests for quantitative and semi-quantitative detection of 4- n -nonylphenol in water was developed. A simple visual dipstick test was based on changing of spot colour from green to brow...
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| Veröffentlicht in: | Analytical and bioanalytical chemistry 2003-04, Vol.375 (8), p.1017-1019 |
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| container_title | Analytical and bioanalytical chemistry |
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| creator | Samsonova, Jeanne V Rubtsova, Maya Yu Franek, Milan |
| description | A range of monoclonal antibody-based competitive immunoassays in the format of microtitre plate ELISA and dipstick tests for quantitative and semi-quantitative detection of 4- n -nonylphenol in water was developed. A simple visual dipstick test was based on changing of spot colour from green to brown in the presence of 4- n -nonylphenol at concentrations within the range 10-100 ng mL(-1). Two different detection systems were used for quantitative immunoassay. Application of enhanced chemiluminescence (ECL) resulted in an increase of the sensitivity of ELISA when compared to conventional colorimetric detection. Thus a detection limit of 0.06 ng mL(-1 )of 4- n -nonylphenol was achieved with IC(50) 2.0 ng mL(-1). The tests developed were applied to natural and spiked water samples. |
| doi_str_mv | 10.1007/s00216-003-1815-3 |
| format | Article |
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| subjects | 4-n-nonylphenol Enzyme-Linked Immunosorbent Assay Immunoenzyme Techniques - methods Nylons Phenols - analysis Sensitivity and Specificity Water - chemistry |
| title | Determination of 4-n-nonylphenol in water by enzyme immunoassay |
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