Neurokinin B Is a Paracrine Vasodilator in the Human Fetal Placental Circulation
Neurokinin (NK) B is a member of the tachykinin family of neurotransmitters, exerting hypotensive or hypertensive effects in the mammalian vasculature through synaptic release from peripheral neurons, according to either NK1 and NK2 or NK3 receptor subtype expression, respectively. There is recent e...
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| Veröffentlicht in: | The journal of clinical endocrinology and metabolism 2003-05, Vol.88 (5), p.2164-2170 |
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| container_title | The journal of clinical endocrinology and metabolism |
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| creator | Brownbill, P. Bell, N. J. Woods, R. J. Lowry, P. J. Page, N. M. Sibley, C. P. |
| description | Neurokinin (NK) B is a member of the tachykinin family of neurotransmitters, exerting hypotensive or hypertensive effects in the mammalian vasculature through synaptic release from peripheral neurons, according to either NK1 and NK2 or NK3 receptor subtype expression, respectively. There is recent evidence that NKB is expressed by the syncytiotrophoblast of the human placenta, an organ that is not innervated. We hypothesized that NKB is a paracrine modulator of tone in the fetal placental circulation. We tested this hypothesis using the in vitro perfused human placental cotyledon. Our data show that NKB is a dilator of the fetal vasculature, causing a maximal 25.1 ± 4.5% (mean ± sem; n = 5) decrease in fetal-side arterial hydrostatic pressure (5-μm NKB bolus; effective concentration in the circulation, 1.89 nm) after preconstriction with U-46619. RT-PCR demonstrated the presence of mRNA for NK1 and NK2 tachykinin receptors in the placenta. Using selective receptor antagonists, we found that NKB-induced vasodilation is through the NK1 receptor subtype. We found no evidence for the involvement of either nitric oxide or prostacyclin in this response. This study demonstrates a paracrine role for NKB in the regulation of fetal placental vascular tone. |
| doi_str_mv | 10.1210/jc.2002-021727 |
| format | Article |
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J. ; Woods, R. J. ; Lowry, P. J. ; Page, N. M. ; Sibley, C. P.</creator><creatorcontrib>Brownbill, P. ; Bell, N. J. ; Woods, R. J. ; Lowry, P. J. ; Page, N. M. ; Sibley, C. P.</creatorcontrib><description>Neurokinin (NK) B is a member of the tachykinin family of neurotransmitters, exerting hypotensive or hypertensive effects in the mammalian vasculature through synaptic release from peripheral neurons, according to either NK1 and NK2 or NK3 receptor subtype expression, respectively. There is recent evidence that NKB is expressed by the syncytiotrophoblast of the human placenta, an organ that is not innervated. We hypothesized that NKB is a paracrine modulator of tone in the fetal placental circulation. We tested this hypothesis using the in vitro perfused human placental cotyledon. Our data show that NKB is a dilator of the fetal vasculature, causing a maximal 25.1 ± 4.5% (mean ± sem; n = 5) decrease in fetal-side arterial hydrostatic pressure (5-μm NKB bolus; effective concentration in the circulation, 1.89 nm) after preconstriction with U-46619. RT-PCR demonstrated the presence of mRNA for NK1 and NK2 tachykinin receptors in the placenta. Using selective receptor antagonists, we found that NKB-induced vasodilation is through the NK1 receptor subtype. We found no evidence for the involvement of either nitric oxide or prostacyclin in this response. This study demonstrates a paracrine role for NKB in the regulation of fetal placental vascular tone.</description><identifier>ISSN: 0021-972X</identifier><identifier>EISSN: 1945-7197</identifier><identifier>DOI: 10.1210/jc.2002-021727</identifier><identifier>PMID: 12727971</identifier><identifier>CODEN: JCEMAZ</identifier><language>eng</language><publisher>Bethesda, MD: Endocrine Society</publisher><subject>Biological and medical sciences ; Blood Pressure - drug effects ; Blood Vessels - drug effects ; Blood Vessels - metabolism ; Cyclooxygenase Inhibitors - pharmacology ; Enzyme Inhibitors - pharmacology ; Epoprostenol - physiology ; Fetus - blood supply ; Fundamental and applied biological sciences. Psychology ; Hormone metabolism and regulation ; Indomethacin - pharmacology ; Neurokinin B - blood ; Neurokinin B - pharmacology ; Neurokinin B - physiology ; NG-Nitroarginine Methyl Ester - pharmacology ; Nitric Oxide - physiology ; Nitric Oxide Synthase - antagonists & inhibitors ; Placenta - blood supply ; Placenta - chemistry ; Pregnancy. Parturition. Lactation ; Receptors, Neurokinin-1 - genetics ; Receptors, Neurokinin-1 - physiology ; Receptors, Neurokinin-2 - genetics ; Receptors, Neurokinin-2 - physiology ; Reverse Transcriptase Polymerase Chain Reaction ; Vasodilation - drug effects ; Vertebrates: reproduction</subject><ispartof>The journal of clinical endocrinology and metabolism, 2003-05, Vol.88 (5), p.2164-2170</ispartof><rights>Copyright © 2003 by The Endocrine Society</rights><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5180-bf731c61759af9dd5f55c5435966949947074483529bf7e22c839fe31d24b47f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14804006$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12727971$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Brownbill, P.</creatorcontrib><creatorcontrib>Bell, N. J.</creatorcontrib><creatorcontrib>Woods, R. J.</creatorcontrib><creatorcontrib>Lowry, P. J.</creatorcontrib><creatorcontrib>Page, N. M.</creatorcontrib><creatorcontrib>Sibley, C. P.</creatorcontrib><title>Neurokinin B Is a Paracrine Vasodilator in the Human Fetal Placental Circulation</title><title>The journal of clinical endocrinology and metabolism</title><addtitle>J Clin Endocrinol Metab</addtitle><description>Neurokinin (NK) B is a member of the tachykinin family of neurotransmitters, exerting hypotensive or hypertensive effects in the mammalian vasculature through synaptic release from peripheral neurons, according to either NK1 and NK2 or NK3 receptor subtype expression, respectively. There is recent evidence that NKB is expressed by the syncytiotrophoblast of the human placenta, an organ that is not innervated. We hypothesized that NKB is a paracrine modulator of tone in the fetal placental circulation. We tested this hypothesis using the in vitro perfused human placental cotyledon. Our data show that NKB is a dilator of the fetal vasculature, causing a maximal 25.1 ± 4.5% (mean ± sem; n = 5) decrease in fetal-side arterial hydrostatic pressure (5-μm NKB bolus; effective concentration in the circulation, 1.89 nm) after preconstriction with U-46619. RT-PCR demonstrated the presence of mRNA for NK1 and NK2 tachykinin receptors in the placenta. Using selective receptor antagonists, we found that NKB-induced vasodilation is through the NK1 receptor subtype. We found no evidence for the involvement of either nitric oxide or prostacyclin in this response. This study demonstrates a paracrine role for NKB in the regulation of fetal placental vascular tone.</description><subject>Biological and medical sciences</subject><subject>Blood Pressure - drug effects</subject><subject>Blood Vessels - drug effects</subject><subject>Blood Vessels - metabolism</subject><subject>Cyclooxygenase Inhibitors - pharmacology</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>Epoprostenol - physiology</subject><subject>Fetus - blood supply</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hormone metabolism and regulation</subject><subject>Indomethacin - pharmacology</subject><subject>Neurokinin B - blood</subject><subject>Neurokinin B - pharmacology</subject><subject>Neurokinin B - physiology</subject><subject>NG-Nitroarginine Methyl Ester - pharmacology</subject><subject>Nitric Oxide - physiology</subject><subject>Nitric Oxide Synthase - antagonists & inhibitors</subject><subject>Placenta - blood supply</subject><subject>Placenta - chemistry</subject><subject>Pregnancy. Parturition. Lactation</subject><subject>Receptors, Neurokinin-1 - genetics</subject><subject>Receptors, Neurokinin-1 - physiology</subject><subject>Receptors, Neurokinin-2 - genetics</subject><subject>Receptors, Neurokinin-2 - physiology</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Vasodilation - drug effects</subject><subject>Vertebrates: reproduction</subject><issn>0021-972X</issn><issn>1945-7197</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kU1vEzEQhi1ERUPhyhH5ArcN_lyvjxC1tFJFcyiIm-V4ZxWnjh3sXVX99zjdSD3VF3tmnndm9BqhT5QsKaPk284tGSGsIYwqpt6gBdVCNopq9RYtaoE2WrG_5-h9KTtCqBCSv0PnlFVYK7pA618w5fTgo4_4B74p2OK1zdZlHwH_sSX1PtgxZVzr4xbw9bS3EV_BaANeB-sgHl8rn91UOZ_iB3Q22FDg4-m-QL-vLu9X183t3c-b1ffbxknakWYzKE5dS5XUdtB9LwcpnRRc6rbVQmuhiBKi45LpigJjruN6AE57JjZCDfwCfZ37HnL6N0EZzd4XByHYCGkqRnEmBCG6gssZdDmVkmEwh-z3Nj8ZSszRQ7Nz5uihmT2sgs-nztNmD_0LfjKtAl9OgC3OhiHb6Hx54URH6uS2cmLmHlMYIZeHMD1CNluwYdwaUo9oVdfU2ZzIGjXHDKkyOcsg9un5Iw4ZSjG7NOVYLX1t7_-DJphV</recordid><startdate>200305</startdate><enddate>200305</enddate><creator>Brownbill, P.</creator><creator>Bell, N. 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Psychology</topic><topic>Hormone metabolism and regulation</topic><topic>Indomethacin - pharmacology</topic><topic>Neurokinin B - blood</topic><topic>Neurokinin B - pharmacology</topic><topic>Neurokinin B - physiology</topic><topic>NG-Nitroarginine Methyl Ester - pharmacology</topic><topic>Nitric Oxide - physiology</topic><topic>Nitric Oxide Synthase - antagonists & inhibitors</topic><topic>Placenta - blood supply</topic><topic>Placenta - chemistry</topic><topic>Pregnancy. Parturition. Lactation</topic><topic>Receptors, Neurokinin-1 - genetics</topic><topic>Receptors, Neurokinin-1 - physiology</topic><topic>Receptors, Neurokinin-2 - genetics</topic><topic>Receptors, Neurokinin-2 - physiology</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Vasodilation - drug effects</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brownbill, P.</creatorcontrib><creatorcontrib>Bell, N. J.</creatorcontrib><creatorcontrib>Woods, R. J.</creatorcontrib><creatorcontrib>Lowry, P. J.</creatorcontrib><creatorcontrib>Page, N. M.</creatorcontrib><creatorcontrib>Sibley, C. P.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The journal of clinical endocrinology and metabolism</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brownbill, P.</au><au>Bell, N. J.</au><au>Woods, R. J.</au><au>Lowry, P. J.</au><au>Page, N. M.</au><au>Sibley, C. P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Neurokinin B Is a Paracrine Vasodilator in the Human Fetal Placental Circulation</atitle><jtitle>The journal of clinical endocrinology and metabolism</jtitle><addtitle>J Clin Endocrinol Metab</addtitle><date>2003-05</date><risdate>2003</risdate><volume>88</volume><issue>5</issue><spage>2164</spage><epage>2170</epage><pages>2164-2170</pages><issn>0021-972X</issn><eissn>1945-7197</eissn><coden>JCEMAZ</coden><abstract>Neurokinin (NK) B is a member of the tachykinin family of neurotransmitters, exerting hypotensive or hypertensive effects in the mammalian vasculature through synaptic release from peripheral neurons, according to either NK1 and NK2 or NK3 receptor subtype expression, respectively. There is recent evidence that NKB is expressed by the syncytiotrophoblast of the human placenta, an organ that is not innervated. We hypothesized that NKB is a paracrine modulator of tone in the fetal placental circulation. We tested this hypothesis using the in vitro perfused human placental cotyledon. Our data show that NKB is a dilator of the fetal vasculature, causing a maximal 25.1 ± 4.5% (mean ± sem; n = 5) decrease in fetal-side arterial hydrostatic pressure (5-μm NKB bolus; effective concentration in the circulation, 1.89 nm) after preconstriction with U-46619. RT-PCR demonstrated the presence of mRNA for NK1 and NK2 tachykinin receptors in the placenta. Using selective receptor antagonists, we found that NKB-induced vasodilation is through the NK1 receptor subtype. We found no evidence for the involvement of either nitric oxide or prostacyclin in this response. This study demonstrates a paracrine role for NKB in the regulation of fetal placental vascular tone.</abstract><cop>Bethesda, MD</cop><pub>Endocrine Society</pub><pmid>12727971</pmid><doi>10.1210/jc.2002-021727</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The journal of clinical endocrinology and metabolism, 2003-05, Vol.88 (5), p.2164-2170 |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals |
| subjects | Biological and medical sciences Blood Pressure - drug effects Blood Vessels - drug effects Blood Vessels - metabolism Cyclooxygenase Inhibitors - pharmacology Enzyme Inhibitors - pharmacology Epoprostenol - physiology Fetus - blood supply Fundamental and applied biological sciences. Psychology Hormone metabolism and regulation Indomethacin - pharmacology Neurokinin B - blood Neurokinin B - pharmacology Neurokinin B - physiology NG-Nitroarginine Methyl Ester - pharmacology Nitric Oxide - physiology Nitric Oxide Synthase - antagonists & inhibitors Placenta - blood supply Placenta - chemistry Pregnancy. Parturition. Lactation Receptors, Neurokinin-1 - genetics Receptors, Neurokinin-1 - physiology Receptors, Neurokinin-2 - genetics Receptors, Neurokinin-2 - physiology Reverse Transcriptase Polymerase Chain Reaction Vasodilation - drug effects Vertebrates: reproduction |
| title | Neurokinin B Is a Paracrine Vasodilator in the Human Fetal Placental Circulation |
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