Detection of H-2 alleles by PCR-RFLP—an alternative to flow cytometry in the screening of transgenic mice

An increasing number of experimental models is based on well-defined transgenic mice in medical and biological research. Particularly in settings in which transgenic recombinants are used, a fast and reliable method is needed to screen for a defined H-2 background. For this purpose, flow cytometry w...

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Veröffentlicht in:	Journal of immunological methods 2003-05, Vol.276 (1), p.197-205
Hauptverfasser:	Klebs, Sven H.G, Hoffmann, Matthias W, Musholt, Petra B, Bayer, Bettina, Musholt, Thomas J
Format:	Artikel
Sprache:	eng
Schlagworte:	Alleles Animals Antibodies, Monoclonal - immunology Base Sequence Biological and medical sciences Biological material (laboratory animals, preparation of biological tracers, etc.) Cross Reactions Flow Cytometry Fundamental and applied biological sciences. Psychology General aspects H-2 antigens H-2 Antigens - genetics H-2 Antigens - immunology Heteroduplex Analysis Histocompatibility antigens MHC Mice Mice, Inbred BALB C Mice, Inbred C57BL Mice, Transgenic - genetics Mice, Transgenic - immunology Polymerase Chain Reaction - methods Polymorphism, Restriction Fragment Length RFLP Species Specificity Transgenic mice
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