expression cDNA library for suppression cloning in yeast mutants, complementation of a yeast his4 mutant, and EST analysis from the symbiotic basidiomycete Hebeloma cylindrosporum

An oriented expression library was constructed from the mycelia of the symbiotic model fungus Hebeloma cylindrosporum in the high-level yeast expression vector pDR196. DNA sequencing of ~500 expressed sequence tags (ESTs) showed that 15% correspond to known genes, two thirds contain sequences with u...

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Veröffentlicht in:	Genome 2003-04, Vol.46 (2), p.177-181
Hauptverfasser:	Wipf, D, Benjdia, M, Rikirsch, E, Zimmermann, S, Tegeder, M, Frommer, W.B
Format:	Artikel
Sprache:	eng
Schlagworte:	Basidiomycota - cytology Basidiomycota - genetics Basidiomycota - growth & development Biosynthesis cDNA libraries Cloning Cloning, Molecular Dehydrogenase Deoxyribonucleic acid DNA DNA, Complementary - metabolism DNA, Fungal - genetics E coli ectomycorrhizae Enzymatic activity Escherichia coli - genetics Expressed Sequence Tags Fungi gene identification Gene Library genes Genes, Fungal genetic complementation genetic techniques and protocols genetic transformation Genetic Vectors Genetics Hebeloma cylindrosporum Histidine - genetics Life Sciences molecular cloning molecular sequence data mutants Mutation Mycorrhizae - genetics Mycorrhizae - growth & development nucleotide sequences Phylogeny Saccharomyces cerevisiae Suppression, Genetic Symbiosis Transformation, Genetic Yeast Yeasts
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