expression cDNA library for suppression cloning in yeast mutants, complementation of a yeast his4 mutant, and EST analysis from the symbiotic basidiomycete Hebeloma cylindrosporum
An oriented expression library was constructed from the mycelia of the symbiotic model fungus Hebeloma cylindrosporum in the high-level yeast expression vector pDR196. DNA sequencing of ~500 expressed sequence tags (ESTs) showed that 15% correspond to known genes, two thirds contain sequences with u...
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| Veröffentlicht in: | Genome 2003-04, Vol.46 (2), p.177-181 |
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| creator | Wipf, D Benjdia, M Rikirsch, E Zimmermann, S Tegeder, M Frommer, W.B |
| description | An oriented expression library was constructed from the mycelia of the symbiotic model fungus Hebeloma cylindrosporum in the high-level yeast expression vector pDR196. DNA sequencing of ~500 expressed sequence tags (ESTs) showed that 15% correspond to known genes, two thirds contain sequences with unknown function, and the remaining 20% showed no significant similarity to any known genes. The ESTs had a GC content between 44 and 56%, with most of them having a GC content of 5254%, which could be correlated with GC contents of fungal genes. The library was successfully used to identify the Hebeloma HIS4 gene by functional complementation of a yeast his4 mutant. Thus, the library may serve as a powerful tool for identification and characterization of mycorrhizal genes by EST analysis and for the identification of ectomycorrhizal genes by means of suppression cloning.Key words: ectomycorrhiza, functional cDNA library, Hebeloma cylindrosporum, yeast complementation. |
| doi_str_mv | 10.1139/g02-121 |
| format | Article |
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DNA sequencing of ~500 expressed sequence tags (ESTs) showed that 15% correspond to known genes, two thirds contain sequences with unknown function, and the remaining 20% showed no significant similarity to any known genes. The ESTs had a GC content between 44 and 56%, with most of them having a GC content of 5254%, which could be correlated with GC contents of fungal genes. The library was successfully used to identify the Hebeloma HIS4 gene by functional complementation of a yeast his4 mutant. Thus, the library may serve as a powerful tool for identification and characterization of mycorrhizal genes by EST analysis and for the identification of ectomycorrhizal genes by means of suppression cloning.Key words: ectomycorrhiza, functional cDNA library, Hebeloma cylindrosporum, yeast complementation.</description><identifier>ISSN: 0831-2796</identifier><identifier>EISSN: 1480-3321</identifier><identifier>DOI: 10.1139/g02-121</identifier><identifier>PMID: 12723033</identifier><identifier>CODEN: GENOE3</identifier><language>eng</language><publisher>Ottawa, Canada: NRC Research Press</publisher><subject>Basidiomycota - cytology ; Basidiomycota - genetics ; Basidiomycota - growth & development ; Biosynthesis ; cDNA libraries ; Cloning ; Cloning, Molecular ; Dehydrogenase ; Deoxyribonucleic acid ; DNA ; DNA, Complementary - metabolism ; DNA, Fungal - genetics ; E coli ; ectomycorrhizae ; Enzymatic activity ; Escherichia coli - genetics ; Expressed Sequence Tags ; Fungi ; gene identification ; Gene Library ; genes ; Genes, Fungal ; genetic complementation ; genetic techniques and protocols ; genetic transformation ; Genetic Vectors ; Genetics ; Hebeloma cylindrosporum ; Histidine - genetics ; Life Sciences ; molecular cloning ; molecular sequence data ; mutants ; Mutation ; Mycorrhizae - genetics ; Mycorrhizae - growth & development ; nucleotide sequences ; Phylogeny ; Saccharomyces cerevisiae ; Suppression, Genetic ; Symbiosis ; Transformation, Genetic ; Yeast ; Yeasts</subject><ispartof>Genome, 2003-04, Vol.46 (2), p.177-181</ispartof><rights>Copyright National Research Council of Canada Apr 2003</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c525t-12d6a80055e81bce40ce6f1cfb26ed9ccc0f889418878369b7447e505d9420c03</citedby><cites>FETCH-LOGICAL-c525t-12d6a80055e81bce40ce6f1cfb26ed9ccc0f889418878369b7447e505d9420c03</cites><orcidid>0000-0001-7197-5612</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://cdnsciencepub.com/doi/pdf/10.1139/g02-121$$EPDF$$P50$$Gnrcresearch$$H</linktopdf><linktohtml>$$Uhttps://cdnsciencepub.com/doi/full/10.1139/g02-121$$EHTML$$P50$$Gnrcresearch$$H</linktohtml><link.rule.ids>230,314,776,780,881,2919,27901,27902,64401,64979</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12723033$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02673862$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Wipf, D</creatorcontrib><creatorcontrib>Benjdia, M</creatorcontrib><creatorcontrib>Rikirsch, E</creatorcontrib><creatorcontrib>Zimmermann, S</creatorcontrib><creatorcontrib>Tegeder, M</creatorcontrib><creatorcontrib>Frommer, W.B</creatorcontrib><title>expression cDNA library for suppression cloning in yeast mutants, complementation of a yeast his4 mutant, and EST analysis from the symbiotic basidiomycete Hebeloma cylindrosporum</title><title>Genome</title><addtitle>Génome</addtitle><description>An oriented expression library was constructed from the mycelia of the symbiotic model fungus Hebeloma cylindrosporum in the high-level yeast expression vector pDR196. DNA sequencing of ~500 expressed sequence tags (ESTs) showed that 15% correspond to known genes, two thirds contain sequences with unknown function, and the remaining 20% showed no significant similarity to any known genes. The ESTs had a GC content between 44 and 56%, with most of them having a GC content of 5254%, which could be correlated with GC contents of fungal genes. The library was successfully used to identify the Hebeloma HIS4 gene by functional complementation of a yeast his4 mutant. Thus, the library may serve as a powerful tool for identification and characterization of mycorrhizal genes by EST analysis and for the identification of ectomycorrhizal genes by means of suppression cloning.Key words: ectomycorrhiza, functional cDNA library, Hebeloma cylindrosporum, yeast complementation.</description><subject>Basidiomycota - cytology</subject><subject>Basidiomycota - genetics</subject><subject>Basidiomycota - growth & development</subject><subject>Biosynthesis</subject><subject>cDNA libraries</subject><subject>Cloning</subject><subject>Cloning, Molecular</subject><subject>Dehydrogenase</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA, Complementary - metabolism</subject><subject>DNA, Fungal - genetics</subject><subject>E coli</subject><subject>ectomycorrhizae</subject><subject>Enzymatic activity</subject><subject>Escherichia coli - genetics</subject><subject>Expressed Sequence Tags</subject><subject>Fungi</subject><subject>gene identification</subject><subject>Gene Library</subject><subject>genes</subject><subject>Genes, Fungal</subject><subject>genetic complementation</subject><subject>genetic techniques and protocols</subject><subject>genetic transformation</subject><subject>Genetic Vectors</subject><subject>Genetics</subject><subject>Hebeloma cylindrosporum</subject><subject>Histidine - genetics</subject><subject>Life Sciences</subject><subject>molecular cloning</subject><subject>molecular sequence data</subject><subject>mutants</subject><subject>Mutation</subject><subject>Mycorrhizae - genetics</subject><subject>Mycorrhizae - growth & development</subject><subject>nucleotide sequences</subject><subject>Phylogeny</subject><subject>Saccharomyces cerevisiae</subject><subject>Suppression, Genetic</subject><subject>Symbiosis</subject><subject>Transformation, Genetic</subject><subject>Yeast</subject><subject>Yeasts</subject><issn>0831-2796</issn><issn>1480-3321</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqF0t2K1DAUB_Aiijuu4hto8EJRtpqT9CO9HNbVEQa92N3rkKanM1mapiat2OfyBc0wZQcU8SqQ_DjncPJPkudA3wPw6sOOshQYPEhWkAmacs7gYbKigkPKyqo4S56EcEcpUF7B4-QMWMk45XyV_MKfg8cQjOuJ_vh1TTpTe-Vn0jpPwjScHjvXm35HTE9mVGEkdhpVP4YLop0dOrTYj2o8SNcStZi9CdkCL4jqG3J1fRNP1c3BBNJ6Z8m4RxJmWxs3Gk1qFUxjnJ01jkg2WGPnrCJ67kzfeBcG5yf7NHnUqi7gs-U8T24_Xd1cbtLtt89fLtfbVOcsH-M-mkIJSvMcBdQaM6qxaEG3NSuwqbTWtBWiykCIUvCiqsssKzGneVNljGrKz5O3x7p71cnBGxv3Ip0ycrPeysMdZUXJRcF-QLSvj3bw7vuEYZTWBI1dp3p0U5AlZxlneflfCKKoeAaH7q_-gHdu8nF3QTJGc6CZyCN6c0Q6Lid4bO_nBCoPyZAxGTImI8oXS7mpttic3BKF02C91_HTUXm9v1dLFTk0bYTv_g3_bvvyiFvlpNp5E-TtNaPAKQBjZcH4bzyV2Xg</recordid><startdate>20030401</startdate><enddate>20030401</enddate><creator>Wipf, D</creator><creator>Benjdia, M</creator><creator>Rikirsch, E</creator><creator>Zimmermann, S</creator><creator>Tegeder, M</creator><creator>Frommer, W.B</creator><general>NRC Research Press</general><general>Canadian Science Publishing NRC Research Press</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7SS</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8FQ</scope><scope>8FV</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M2P</scope><scope>M3G</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>M7N</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0001-7197-5612</orcidid></search><sort><creationdate>20030401</creationdate><title>expression cDNA library for suppression cloning in yeast mutants, complementation of a yeast his4 mutant, and EST analysis from the symbiotic basidiomycete Hebeloma cylindrosporum</title><author>Wipf, D ; Benjdia, M ; Rikirsch, E ; Zimmermann, S ; Tegeder, M ; Frommer, W.B</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c525t-12d6a80055e81bce40ce6f1cfb26ed9ccc0f889418878369b7447e505d9420c03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Basidiomycota - 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genetics</topic><topic>Mycorrhizae - growth & development</topic><topic>nucleotide sequences</topic><topic>Phylogeny</topic><topic>Saccharomyces cerevisiae</topic><topic>Suppression, Genetic</topic><topic>Symbiosis</topic><topic>Transformation, Genetic</topic><topic>Yeast</topic><topic>Yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wipf, D</creatorcontrib><creatorcontrib>Benjdia, M</creatorcontrib><creatorcontrib>Rikirsch, E</creatorcontrib><creatorcontrib>Zimmermann, S</creatorcontrib><creatorcontrib>Tegeder, M</creatorcontrib><creatorcontrib>Frommer, W.B</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Canadian Business & Current Affairs Database</collection><collection>Canadian Business & Current Affairs Database (Alumni Edition)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Science Database</collection><collection>CBCA Reference & Current Events</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Genome</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wipf, D</au><au>Benjdia, M</au><au>Rikirsch, E</au><au>Zimmermann, S</au><au>Tegeder, M</au><au>Frommer, W.B</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>expression cDNA library for suppression cloning in yeast mutants, complementation of a yeast his4 mutant, and EST analysis from the symbiotic basidiomycete Hebeloma cylindrosporum</atitle><jtitle>Genome</jtitle><addtitle>Génome</addtitle><date>2003-04-01</date><risdate>2003</risdate><volume>46</volume><issue>2</issue><spage>177</spage><epage>181</epage><pages>177-181</pages><issn>0831-2796</issn><eissn>1480-3321</eissn><coden>GENOE3</coden><abstract>An oriented expression library was constructed from the mycelia of the symbiotic model fungus Hebeloma cylindrosporum in the high-level yeast expression vector pDR196. DNA sequencing of ~500 expressed sequence tags (ESTs) showed that 15% correspond to known genes, two thirds contain sequences with unknown function, and the remaining 20% showed no significant similarity to any known genes. The ESTs had a GC content between 44 and 56%, with most of them having a GC content of 5254%, which could be correlated with GC contents of fungal genes. The library was successfully used to identify the Hebeloma HIS4 gene by functional complementation of a yeast his4 mutant. Thus, the library may serve as a powerful tool for identification and characterization of mycorrhizal genes by EST analysis and for the identification of ectomycorrhizal genes by means of suppression cloning.Key words: ectomycorrhiza, functional cDNA library, Hebeloma cylindrosporum, yeast complementation.</abstract><cop>Ottawa, Canada</cop><pub>NRC Research Press</pub><pmid>12723033</pmid><doi>10.1139/g02-121</doi><tpages>5</tpages><orcidid>https://orcid.org/0000-0001-7197-5612</orcidid></addata></record> |
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| subjects | Basidiomycota - cytology Basidiomycota - genetics Basidiomycota - growth & development Biosynthesis cDNA libraries Cloning Cloning, Molecular Dehydrogenase Deoxyribonucleic acid DNA DNA, Complementary - metabolism DNA, Fungal - genetics E coli ectomycorrhizae Enzymatic activity Escherichia coli - genetics Expressed Sequence Tags Fungi gene identification Gene Library genes Genes, Fungal genetic complementation genetic techniques and protocols genetic transformation Genetic Vectors Genetics Hebeloma cylindrosporum Histidine - genetics Life Sciences molecular cloning molecular sequence data mutants Mutation Mycorrhizae - genetics Mycorrhizae - growth & development nucleotide sequences Phylogeny Saccharomyces cerevisiae Suppression, Genetic Symbiosis Transformation, Genetic Yeast Yeasts |
| title | expression cDNA library for suppression cloning in yeast mutants, complementation of a yeast his4 mutant, and EST analysis from the symbiotic basidiomycete Hebeloma cylindrosporum |
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