Expression of heat shock genes during differentiation of mammalian osteoblasts and promyelocytic leukemia cells
The progressive differentiation of both normal rat osteoblasts and HL‐60 promyelocytic leukemia cells involves the sequential expression of specific genes encoding proteins that are characteristic of their respective developing cellular phenotypes. In addition to the selective expression of various...
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| Veröffentlicht in: | Journal of cellular biochemistry 1992-03, Vol.48 (3), p.277-287 |
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| container_title | Journal of cellular biochemistry |
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| creator | Shakoori, Abdul Rauf Oberdorf, Annette M. Owen, Thomas A. Weber, Lee A. Hickey, Eileen Stein, Janet L. Lian, Jane B. Stein, Gary S. |
| description | The progressive differentiation of both normal rat osteoblasts and HL‐60 promyelocytic leukemia cells involves the sequential expression of specific genes encoding proteins that are characteristic of their respective developing cellular phenotypes. In addition to the selective expression of various phenotype marker genes, several members of the heat shock gene family exhibit differential expression throughout the developmental sequence of these two cell types. As determined by steady state mRNA levels, in both osteoblasts and HL‐60 cells expression of hsp27, hsp60, hsp70, hsp89α, and hsp89β may be associated with the modifications in gene expression and cellular architecture that occur during differentiation.
In both differentiation systems, the expression of hsp27 mRNA shows a 2.5‐fold increase with the down‐regulation of proliferation while hsp60 mRNA levels are maximal during active proliferation and subsequently decline post‐proliferatively. mRNA expression of two members of the hsp90 family decreases with the shutdown of proliferation, with a parallel relationship between hsp89α mRNA levels and proliferation in osteoblasts and a delay in down‐regulation of hsp89α mRNA levels in HL‐60 cells and of hsp89β mRNA in both systems. Hsp70 mRNA rapidly increases, almost twofold, as proliferation decreases in HL‐60 cells but during osteoblast growth and differentiation was only minimally detectable and showed no significant changes. Although the presence of the various hsp mRNA species is maintained at some level throughout the developmental sequence of both osteoblasts and HL‐60 cells, changes in the extent to which the heat shock genes are expressed occur primarily in association with the decline of proliferative activity. The observed differences in patterns of expression for the various heat shock genes are consistent with involvement in mediating a series of regulatory events functionally related to the control of both cell growth and differentiation. |
| doi_str_mv | 10.1002/jcb.240480308 |
| format | Article |
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In both differentiation systems, the expression of hsp27 mRNA shows a 2.5‐fold increase with the down‐regulation of proliferation while hsp60 mRNA levels are maximal during active proliferation and subsequently decline post‐proliferatively. mRNA expression of two members of the hsp90 family decreases with the shutdown of proliferation, with a parallel relationship between hsp89α mRNA levels and proliferation in osteoblasts and a delay in down‐regulation of hsp89α mRNA levels in HL‐60 cells and of hsp89β mRNA in both systems. Hsp70 mRNA rapidly increases, almost twofold, as proliferation decreases in HL‐60 cells but during osteoblast growth and differentiation was only minimally detectable and showed no significant changes. Although the presence of the various hsp mRNA species is maintained at some level throughout the developmental sequence of both osteoblasts and HL‐60 cells, changes in the extent to which the heat shock genes are expressed occur primarily in association with the decline of proliferative activity. The observed differences in patterns of expression for the various heat shock genes are consistent with involvement in mediating a series of regulatory events functionally related to the control of both cell growth and differentiation.</description><identifier>ISSN: 0730-2312</identifier><identifier>EISSN: 1097-4644</identifier><identifier>DOI: 10.1002/jcb.240480308</identifier><identifier>PMID: 1400614</identifier><identifier>CODEN: JCEBD5</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animals ; Biological and medical sciences ; Blotting, Northern ; bone ; Cell Differentiation - genetics ; Cell differentiation, maturation, development, hematopoiesis ; Cell physiology ; Cells, Cultured ; Fundamental and applied biological sciences. Psychology ; gene regulation ; Heat-Shock Proteins - genetics ; HL-60 cells ; hsp27 ; hsp60 ; hsp70 ; hsp89α ; hsp89β ; Humans ; Kinetics ; Leukemia, Promyelocytic, Acute ; Molecular and cellular biology ; Osteoblasts - cytology ; Osteoblasts - metabolism ; proliferation ; Rats ; RNA, Messenger - genetics ; Tumor Cells, Cultured</subject><ispartof>Journal of cellular biochemistry, 1992-03, Vol.48 (3), p.277-287</ispartof><rights>Copyright © 1992 Wiley‐Liss, Inc.</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5008-b40cf16a80819ce01c2c75eeb2f832542cead0f4ae90e753600b868393a26a573</citedby><cites>FETCH-LOGICAL-c5008-b40cf16a80819ce01c2c75eeb2f832542cead0f4ae90e753600b868393a26a573</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjcb.240480308$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjcb.240480308$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,777,781,1412,27905,27906,45555,45556</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5381171$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1400614$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shakoori, Abdul Rauf</creatorcontrib><creatorcontrib>Oberdorf, Annette M.</creatorcontrib><creatorcontrib>Owen, Thomas A.</creatorcontrib><creatorcontrib>Weber, Lee A.</creatorcontrib><creatorcontrib>Hickey, Eileen</creatorcontrib><creatorcontrib>Stein, Janet L.</creatorcontrib><creatorcontrib>Lian, Jane B.</creatorcontrib><creatorcontrib>Stein, Gary S.</creatorcontrib><title>Expression of heat shock genes during differentiation of mammalian osteoblasts and promyelocytic leukemia cells</title><title>Journal of cellular biochemistry</title><addtitle>J. Cell. Biochem</addtitle><description>The progressive differentiation of both normal rat osteoblasts and HL‐60 promyelocytic leukemia cells involves the sequential expression of specific genes encoding proteins that are characteristic of their respective developing cellular phenotypes. In addition to the selective expression of various phenotype marker genes, several members of the heat shock gene family exhibit differential expression throughout the developmental sequence of these two cell types. As determined by steady state mRNA levels, in both osteoblasts and HL‐60 cells expression of hsp27, hsp60, hsp70, hsp89α, and hsp89β may be associated with the modifications in gene expression and cellular architecture that occur during differentiation.
In both differentiation systems, the expression of hsp27 mRNA shows a 2.5‐fold increase with the down‐regulation of proliferation while hsp60 mRNA levels are maximal during active proliferation and subsequently decline post‐proliferatively. mRNA expression of two members of the hsp90 family decreases with the shutdown of proliferation, with a parallel relationship between hsp89α mRNA levels and proliferation in osteoblasts and a delay in down‐regulation of hsp89α mRNA levels in HL‐60 cells and of hsp89β mRNA in both systems. Hsp70 mRNA rapidly increases, almost twofold, as proliferation decreases in HL‐60 cells but during osteoblast growth and differentiation was only minimally detectable and showed no significant changes. Although the presence of the various hsp mRNA species is maintained at some level throughout the developmental sequence of both osteoblasts and HL‐60 cells, changes in the extent to which the heat shock genes are expressed occur primarily in association with the decline of proliferative activity. The observed differences in patterns of expression for the various heat shock genes are consistent with involvement in mediating a series of regulatory events functionally related to the control of both cell growth and differentiation.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern</subject><subject>bone</subject><subject>Cell Differentiation - genetics</subject><subject>Cell differentiation, maturation, development, hematopoiesis</subject><subject>Cell physiology</subject><subject>Cells, Cultured</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene regulation</subject><subject>Heat-Shock Proteins - genetics</subject><subject>HL-60 cells</subject><subject>hsp27</subject><subject>hsp60</subject><subject>hsp70</subject><subject>hsp89α</subject><subject>hsp89β</subject><subject>Humans</subject><subject>Kinetics</subject><subject>Leukemia, Promyelocytic, Acute</subject><subject>Molecular and cellular biology</subject><subject>Osteoblasts - cytology</subject><subject>Osteoblasts - metabolism</subject><subject>proliferation</subject><subject>Rats</subject><subject>RNA, Messenger - genetics</subject><subject>Tumor Cells, Cultured</subject><issn>0730-2312</issn><issn>1097-4644</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1v1DAQxS0EKtvCkSOSD4hbyvgjsXOkUWmBAhcQEhdr4p207uZjsRPR_e_JaqOFE5xGo_nNm6d5jL0QcC4A5Jt7X59LDdqCAvuIrQSUJtOF1o_ZCoyCTCohn7LTlO4BoCyVPGEnQgMUQq_YcPmwjZRSGHo-NPyOcOTpbvAbfks9Jb6eYuhv-To0DUXqx4DjgnbYddgGnJs00lC3mMbEsV_zbRy6HbWD343B85amDXUBuae2Tc_YkwbbRM-Xesa-vbv8Wl1nN1-u3ldvbzKfA9is1uAbUaAFK0pPILz0JieqZWOVzLX0hGtoNFIJZHJVANS2sKpUKAvMjTpjrw-6s5mfE6XRdSHtHWBPw5ScUVKVprT_BUVRGLBSzGB2AH0cUorUuG0MHcadE-D2Sbg5CXdMYuZfLsJT3dH6D314_Tx_tcwxeWybiL0P6Yjlygph9mfNAfsVWtr9-6b7UF38bWAxHOZ8Ho6bGDeuMMrk7vvnK_cp_1FV5mPlLtRvI42xOQ</recordid><startdate>199203</startdate><enddate>199203</enddate><creator>Shakoori, Abdul Rauf</creator><creator>Oberdorf, Annette M.</creator><creator>Owen, Thomas A.</creator><creator>Weber, Lee A.</creator><creator>Hickey, Eileen</creator><creator>Stein, Janet L.</creator><creator>Lian, Jane B.</creator><creator>Stein, Gary S.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>199203</creationdate><title>Expression of heat shock genes during differentiation of mammalian osteoblasts and promyelocytic leukemia cells</title><author>Shakoori, Abdul Rauf ; Oberdorf, Annette M. ; Owen, Thomas A. ; Weber, Lee A. ; Hickey, Eileen ; Stein, Janet L. ; Lian, Jane B. ; Stein, Gary S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5008-b40cf16a80819ce01c2c75eeb2f832542cead0f4ae90e753600b868393a26a573</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blotting, Northern</topic><topic>bone</topic><topic>Cell Differentiation - genetics</topic><topic>Cell differentiation, maturation, development, hematopoiesis</topic><topic>Cell physiology</topic><topic>Cells, Cultured</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gene regulation</topic><topic>Heat-Shock Proteins - genetics</topic><topic>HL-60 cells</topic><topic>hsp27</topic><topic>hsp60</topic><topic>hsp70</topic><topic>hsp89α</topic><topic>hsp89β</topic><topic>Humans</topic><topic>Kinetics</topic><topic>Leukemia, Promyelocytic, Acute</topic><topic>Molecular and cellular biology</topic><topic>Osteoblasts - cytology</topic><topic>Osteoblasts - metabolism</topic><topic>proliferation</topic><topic>Rats</topic><topic>RNA, Messenger - genetics</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shakoori, Abdul Rauf</creatorcontrib><creatorcontrib>Oberdorf, Annette M.</creatorcontrib><creatorcontrib>Owen, Thomas A.</creatorcontrib><creatorcontrib>Weber, Lee A.</creatorcontrib><creatorcontrib>Hickey, Eileen</creatorcontrib><creatorcontrib>Stein, Janet L.</creatorcontrib><creatorcontrib>Lian, Jane B.</creatorcontrib><creatorcontrib>Stein, Gary S.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of cellular biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shakoori, Abdul Rauf</au><au>Oberdorf, Annette M.</au><au>Owen, Thomas A.</au><au>Weber, Lee A.</au><au>Hickey, Eileen</au><au>Stein, Janet L.</au><au>Lian, Jane B.</au><au>Stein, Gary S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of heat shock genes during differentiation of mammalian osteoblasts and promyelocytic leukemia cells</atitle><jtitle>Journal of cellular biochemistry</jtitle><addtitle>J. Cell. Biochem</addtitle><date>1992-03</date><risdate>1992</risdate><volume>48</volume><issue>3</issue><spage>277</spage><epage>287</epage><pages>277-287</pages><issn>0730-2312</issn><eissn>1097-4644</eissn><coden>JCEBD5</coden><abstract>The progressive differentiation of both normal rat osteoblasts and HL‐60 promyelocytic leukemia cells involves the sequential expression of specific genes encoding proteins that are characteristic of their respective developing cellular phenotypes. In addition to the selective expression of various phenotype marker genes, several members of the heat shock gene family exhibit differential expression throughout the developmental sequence of these two cell types. As determined by steady state mRNA levels, in both osteoblasts and HL‐60 cells expression of hsp27, hsp60, hsp70, hsp89α, and hsp89β may be associated with the modifications in gene expression and cellular architecture that occur during differentiation.
In both differentiation systems, the expression of hsp27 mRNA shows a 2.5‐fold increase with the down‐regulation of proliferation while hsp60 mRNA levels are maximal during active proliferation and subsequently decline post‐proliferatively. mRNA expression of two members of the hsp90 family decreases with the shutdown of proliferation, with a parallel relationship between hsp89α mRNA levels and proliferation in osteoblasts and a delay in down‐regulation of hsp89α mRNA levels in HL‐60 cells and of hsp89β mRNA in both systems. Hsp70 mRNA rapidly increases, almost twofold, as proliferation decreases in HL‐60 cells but during osteoblast growth and differentiation was only minimally detectable and showed no significant changes. Although the presence of the various hsp mRNA species is maintained at some level throughout the developmental sequence of both osteoblasts and HL‐60 cells, changes in the extent to which the heat shock genes are expressed occur primarily in association with the decline of proliferative activity. The observed differences in patterns of expression for the various heat shock genes are consistent with involvement in mediating a series of regulatory events functionally related to the control of both cell growth and differentiation.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>1400614</pmid><doi>10.1002/jcb.240480308</doi><tpages>11</tpages></addata></record> |
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| ispartof | Journal of cellular biochemistry, 1992-03, Vol.48 (3), p.277-287 |
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| subjects | Animals Biological and medical sciences Blotting, Northern bone Cell Differentiation - genetics Cell differentiation, maturation, development, hematopoiesis Cell physiology Cells, Cultured Fundamental and applied biological sciences. Psychology gene regulation Heat-Shock Proteins - genetics HL-60 cells hsp27 hsp60 hsp70 hsp89α hsp89β Humans Kinetics Leukemia, Promyelocytic, Acute Molecular and cellular biology Osteoblasts - cytology Osteoblasts - metabolism proliferation Rats RNA, Messenger - genetics Tumor Cells, Cultured |
| title | Expression of heat shock genes during differentiation of mammalian osteoblasts and promyelocytic leukemia cells |
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