Thermodynamic and Kinetic Analysis of a Peptide−Class I MHC Interaction Highlights the Noncovalent Nature and Conformational Dynamics of the Class I Heterotrimer

The class I major histocompatibility (MHC) molecule is a heterotrimer composed of a heavy chain, the small subunit β2-microglobulin (β2m), and a peptide. Fluorescence anisotropy has been used to assay the interaction of a labeled peptide with a recombinant, soluble form of the class I MHC HLA-A2. Co...

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Veröffentlicht in:	Biochemistry (Easton) 2003-05, Vol.42 (17), p.4954-4961
Hauptverfasser:	Binz, Anne-Kathrin, Rodriguez, Rene C, Biddison, William E, Baker, Brian M
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Binding Sites Calorimetry Cloning, Molecular Dimerization Escherichia coli - genetics HLA-A2 Antigen - chemistry HLA-A2 Antigen - genetics Kinetics Macromolecular Substances Peptide Fragments - chemistry Protein Conformation Protein Denaturation Recombinant Proteins - chemistry Spectroscopy, Fourier Transform Infrared Thermodynamics
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