Diagnostic Significance and Clinical Applications of Chimeric Genes in Ewing's Sarcoma
Ewing's sarcoma (ES) is one of the most malignant bone and soft tissue tumors in childhood. Morphologically, ES belongs to the small round cell tumors (SRCT). ES, peripheral primitive neuroectodermal tumor (PNET), and Askin's tumor are classified as ES family tumors (ESFT) because they sha...
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| Veröffentlicht in: | Biological & Pharmaceutical Bulletin 2003, Vol.26(5), pp.585-588 |
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| creator | Yoshino, Naoko Kojima, Tetsuko Asami, Satoru Motohashi, Shigeyasu Yoshida, Yoshikazu Chin, Motoaki Shichino, Hiroyuki Yoshida, Yukihiro Nemoto, Norimichi Kaneko, Michio Mugishima, Hideo Suzuki, Takashi |
| description | Ewing's sarcoma (ES) is one of the most malignant bone and soft tissue tumors in childhood. Morphologically, ES belongs to the small round cell tumors (SRCT). ES, peripheral primitive neuroectodermal tumor (PNET), and Askin's tumor are classified as ES family tumors (ESFT) because they share a common chromosomal translocation. The EWS-FLI1 chimeric gene is generated by t (11; 22). Other reciprocal translocations resulting in formation of chimeric genes between EWS and ETS family genes (ERG, ETV1, E1AF, and FEV) are t (21; 22), t (7; 22), t (17; 22), and t (2; 22), respectively. Although it is generally difficult to distinguish ES from SRCT, we could easily and quickly distinguish ES from other SRCT by using reverse transcription polymerase chain reaction (RT-PCR). We looked for specific chimeric genes in 23 tumor samples, including three ES clinical samples. We detected five chimeric genes in the three ES samples. Three chimeric genes, all EWS-FLI1, were detected in one ES sample. Different chimeric genes, EWS-ERG and EWS-ETV1, were detected in the other two ES samples. Moreover, because we could not detect specific chimeric genes in samples from non-ESFT, it may be possible to use this technique to diagnose ESFT and to detect tumor cell contamination before hematopoietic stem cell transplantation. |
| doi_str_mv | 10.1248/bpb.26.585 |
| format | Article |
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Morphologically, ES belongs to the small round cell tumors (SRCT). ES, peripheral primitive neuroectodermal tumor (PNET), and Askin's tumor are classified as ES family tumors (ESFT) because they share a common chromosomal translocation. The EWS-FLI1 chimeric gene is generated by t (11; 22). Other reciprocal translocations resulting in formation of chimeric genes between EWS and ETS family genes (ERG, ETV1, E1AF, and FEV) are t (21; 22), t (7; 22), t (17; 22), and t (2; 22), respectively. Although it is generally difficult to distinguish ES from SRCT, we could easily and quickly distinguish ES from other SRCT by using reverse transcription polymerase chain reaction (RT-PCR). We looked for specific chimeric genes in 23 tumor samples, including three ES clinical samples. We detected five chimeric genes in the three ES samples. Three chimeric genes, all EWS-FLI1, were detected in one ES sample. Different chimeric genes, EWS-ERG and EWS-ETV1, were detected in the other two ES samples. Moreover, because we could not detect specific chimeric genes in samples from non-ESFT, it may be possible to use this technique to diagnose ESFT and to detect tumor cell contamination before hematopoietic stem cell transplantation.</description><identifier>ISSN: 0918-6158</identifier><identifier>EISSN: 1347-5215</identifier><identifier>DOI: 10.1248/bpb.26.585</identifier><identifier>PMID: 12736494</identifier><language>eng</language><publisher>Tokyo: The Pharmaceutical Society of Japan</publisher><subject>Adolescent ; Biological and medical sciences ; Cell Line, Tumor ; Child ; Child, Preschool ; chimeric gene ; Ewing's sarcoma ; Female ; Humans ; Infant ; Male ; Medical sciences ; Oncogene Proteins, Fusion - genetics ; Reverse Transcriptase Polymerase Chain Reaction ; reverse transcription polymerase chain reaction ; Sarcoma, Ewing - diagnosis ; Sarcoma, Ewing - genetics ; Tumor Cells, Cultured</subject><ispartof>Biological and Pharmaceutical Bulletin, 2003, Vol.26(5), pp.585-588</ispartof><rights>2003 The Pharmaceutical Society of Japan</rights><rights>2003 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 2003</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c713t-1c147f75a02dd5f6812b209939455f944bcef84fd47ae913ff935ef129ddcaa73</citedby><cites>FETCH-LOGICAL-c713t-1c147f75a02dd5f6812b209939455f944bcef84fd47ae913ff935ef129ddcaa73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1877,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14863268$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12736494$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yoshino, Naoko</creatorcontrib><creatorcontrib>Kojima, Tetsuko</creatorcontrib><creatorcontrib>Asami, Satoru</creatorcontrib><creatorcontrib>Motohashi, Shigeyasu</creatorcontrib><creatorcontrib>Yoshida, Yoshikazu</creatorcontrib><creatorcontrib>Chin, Motoaki</creatorcontrib><creatorcontrib>Shichino, Hiroyuki</creatorcontrib><creatorcontrib>Yoshida, Yukihiro</creatorcontrib><creatorcontrib>Nemoto, Norimichi</creatorcontrib><creatorcontrib>Kaneko, Michio</creatorcontrib><creatorcontrib>Mugishima, Hideo</creatorcontrib><creatorcontrib>Suzuki, Takashi</creatorcontrib><creatorcontrib>School of Medicine</creatorcontrib><creatorcontrib>bDepartment of Hospital Pharmacy</creatorcontrib><creatorcontrib>Division of Cell Regeneration and Transplantation</creatorcontrib><creatorcontrib>dDepartment of Orthopedics</creatorcontrib><creatorcontrib>Institute of Clinical Medicine</creatorcontrib><creatorcontrib>aClinical Pharmacy</creatorcontrib><creatorcontrib>eDepartment of Pathology</creatorcontrib><creatorcontrib>School of Pediatric Surgery</creatorcontrib><creatorcontrib>College of Pharmacy</creatorcontrib><creatorcontrib>Nihon University</creatorcontrib><creatorcontrib>University of Tsukuba</creatorcontrib><creatorcontrib>cDepartment of Advanced Medicine</creatorcontrib><title>Diagnostic Significance and Clinical Applications of Chimeric Genes in Ewing's Sarcoma</title><title>Biological & Pharmaceutical Bulletin</title><addtitle>Biol Pharm Bull</addtitle><description>Ewing's sarcoma (ES) is one of the most malignant bone and soft tissue tumors in childhood. Morphologically, ES belongs to the small round cell tumors (SRCT). ES, peripheral primitive neuroectodermal tumor (PNET), and Askin's tumor are classified as ES family tumors (ESFT) because they share a common chromosomal translocation. The EWS-FLI1 chimeric gene is generated by t (11; 22). Other reciprocal translocations resulting in formation of chimeric genes between EWS and ETS family genes (ERG, ETV1, E1AF, and FEV) are t (21; 22), t (7; 22), t (17; 22), and t (2; 22), respectively. Although it is generally difficult to distinguish ES from SRCT, we could easily and quickly distinguish ES from other SRCT by using reverse transcription polymerase chain reaction (RT-PCR). We looked for specific chimeric genes in 23 tumor samples, including three ES clinical samples. We detected five chimeric genes in the three ES samples. Three chimeric genes, all EWS-FLI1, were detected in one ES sample. Different chimeric genes, EWS-ERG and EWS-ETV1, were detected in the other two ES samples. Moreover, because we could not detect specific chimeric genes in samples from non-ESFT, it may be possible to use this technique to diagnose ESFT and to detect tumor cell contamination before hematopoietic stem cell transplantation.</description><subject>Adolescent</subject><subject>Biological and medical sciences</subject><subject>Cell Line, Tumor</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>chimeric gene</subject><subject>Ewing's sarcoma</subject><subject>Female</subject><subject>Humans</subject><subject>Infant</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Oncogene Proteins, Fusion - genetics</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>reverse transcription polymerase chain reaction</subject><subject>Sarcoma, Ewing - diagnosis</subject><subject>Sarcoma, Ewing - genetics</subject><subject>Tumor Cells, Cultured</subject><issn>0918-6158</issn><issn>1347-5215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkV-L1DAUxYMo7rj64geQgqggdMzfpnmSZVxHYcGHVV9DmiazGdqkm3QQv7137LgDvtzckN89OZyL0EuC14Ty9kM3dWvarEUrHqEVYVzWghLxGK2wIm3dENFeoGel7DHGElP2FF0QKlnDFV-hn5-C2cVU5mCr27CLwQdronWViX21GUKE61BdTdMAzRxSLFXy1eYujC7DyNZFV6oQq-tfIe7elerWZJtG8xw98WYo7sXpvEQ_Pl9_33ypb75tv26ubmorCZtrYgmXXgqDad8L37SEdhQrxRQXwivOO-t8y33PpXGKMO8VE84TqvreGiPZJXq76E453R9cmfUYinXDYKJLh6Ilo0wo0gD4-j9wnw45gjdNOFcMM0IYUO8XyuZUSnZeTzmMJv_WBOtj1hqy1rTRkDXAr06Sh250_Rk9hQvAmxNgCqToMwQbypnjbcNo0wK3XThQOcadIuTuzgZtkV1IQ9IUY6Yxpg0W-m8LNo6lJVhK2DUofVyU9mU2O_fwlcmw38E9uF_Kcfrfi70zWbvI_gClhrLX</recordid><startdate>2003</startdate><enddate>2003</enddate><creator>Yoshino, Naoko</creator><creator>Kojima, Tetsuko</creator><creator>Asami, Satoru</creator><creator>Motohashi, Shigeyasu</creator><creator>Yoshida, Yoshikazu</creator><creator>Chin, Motoaki</creator><creator>Shichino, Hiroyuki</creator><creator>Yoshida, Yukihiro</creator><creator>Nemoto, Norimichi</creator><creator>Kaneko, Michio</creator><creator>Mugishima, Hideo</creator><creator>Suzuki, Takashi</creator><general>The Pharmaceutical Society of Japan</general><general>Pharmaceutical Society of Japan</general><general>Maruzen</general><general>Japan Science and Technology Agency</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>2003</creationdate><title>Diagnostic Significance and Clinical Applications of Chimeric Genes in Ewing's Sarcoma</title><author>Yoshino, Naoko ; Kojima, Tetsuko ; Asami, Satoru ; Motohashi, Shigeyasu ; Yoshida, Yoshikazu ; Chin, Motoaki ; Shichino, Hiroyuki ; Yoshida, Yukihiro ; Nemoto, Norimichi ; Kaneko, Michio ; Mugishima, Hideo ; Suzuki, Takashi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c713t-1c147f75a02dd5f6812b209939455f944bcef84fd47ae913ff935ef129ddcaa73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Adolescent</topic><topic>Biological and medical sciences</topic><topic>Cell Line, Tumor</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>chimeric gene</topic><topic>Ewing's sarcoma</topic><topic>Female</topic><topic>Humans</topic><topic>Infant</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Oncogene Proteins, Fusion - genetics</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>reverse transcription polymerase chain reaction</topic><topic>Sarcoma, Ewing - diagnosis</topic><topic>Sarcoma, Ewing - genetics</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yoshino, Naoko</creatorcontrib><creatorcontrib>Kojima, Tetsuko</creatorcontrib><creatorcontrib>Asami, Satoru</creatorcontrib><creatorcontrib>Motohashi, Shigeyasu</creatorcontrib><creatorcontrib>Yoshida, Yoshikazu</creatorcontrib><creatorcontrib>Chin, Motoaki</creatorcontrib><creatorcontrib>Shichino, Hiroyuki</creatorcontrib><creatorcontrib>Yoshida, Yukihiro</creatorcontrib><creatorcontrib>Nemoto, Norimichi</creatorcontrib><creatorcontrib>Kaneko, Michio</creatorcontrib><creatorcontrib>Mugishima, Hideo</creatorcontrib><creatorcontrib>Suzuki, Takashi</creatorcontrib><creatorcontrib>School of Medicine</creatorcontrib><creatorcontrib>bDepartment of Hospital Pharmacy</creatorcontrib><creatorcontrib>Division of Cell Regeneration and Transplantation</creatorcontrib><creatorcontrib>dDepartment of Orthopedics</creatorcontrib><creatorcontrib>Institute of Clinical Medicine</creatorcontrib><creatorcontrib>aClinical Pharmacy</creatorcontrib><creatorcontrib>eDepartment of Pathology</creatorcontrib><creatorcontrib>School of Pediatric Surgery</creatorcontrib><creatorcontrib>College of Pharmacy</creatorcontrib><creatorcontrib>Nihon University</creatorcontrib><creatorcontrib>University of Tsukuba</creatorcontrib><creatorcontrib>cDepartment of Advanced Medicine</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biological & Pharmaceutical Bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yoshino, Naoko</au><au>Kojima, Tetsuko</au><au>Asami, Satoru</au><au>Motohashi, Shigeyasu</au><au>Yoshida, Yoshikazu</au><au>Chin, Motoaki</au><au>Shichino, Hiroyuki</au><au>Yoshida, Yukihiro</au><au>Nemoto, Norimichi</au><au>Kaneko, Michio</au><au>Mugishima, Hideo</au><au>Suzuki, Takashi</au><aucorp>School of Medicine</aucorp><aucorp>bDepartment of Hospital Pharmacy</aucorp><aucorp>Division of Cell Regeneration and Transplantation</aucorp><aucorp>dDepartment of Orthopedics</aucorp><aucorp>Institute of Clinical Medicine</aucorp><aucorp>aClinical Pharmacy</aucorp><aucorp>eDepartment of Pathology</aucorp><aucorp>School of Pediatric Surgery</aucorp><aucorp>College of Pharmacy</aucorp><aucorp>Nihon University</aucorp><aucorp>University of Tsukuba</aucorp><aucorp>cDepartment of Advanced Medicine</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Diagnostic Significance and Clinical Applications of Chimeric Genes in Ewing's Sarcoma</atitle><jtitle>Biological & Pharmaceutical Bulletin</jtitle><addtitle>Biol Pharm Bull</addtitle><date>2003</date><risdate>2003</risdate><volume>26</volume><issue>5</issue><spage>585</spage><epage>588</epage><pages>585-588</pages><issn>0918-6158</issn><eissn>1347-5215</eissn><abstract>Ewing's sarcoma (ES) is one of the most malignant bone and soft tissue tumors in childhood. Morphologically, ES belongs to the small round cell tumors (SRCT). ES, peripheral primitive neuroectodermal tumor (PNET), and Askin's tumor are classified as ES family tumors (ESFT) because they share a common chromosomal translocation. The EWS-FLI1 chimeric gene is generated by t (11; 22). Other reciprocal translocations resulting in formation of chimeric genes between EWS and ETS family genes (ERG, ETV1, E1AF, and FEV) are t (21; 22), t (7; 22), t (17; 22), and t (2; 22), respectively. Although it is generally difficult to distinguish ES from SRCT, we could easily and quickly distinguish ES from other SRCT by using reverse transcription polymerase chain reaction (RT-PCR). We looked for specific chimeric genes in 23 tumor samples, including three ES clinical samples. We detected five chimeric genes in the three ES samples. Three chimeric genes, all EWS-FLI1, were detected in one ES sample. Different chimeric genes, EWS-ERG and EWS-ETV1, were detected in the other two ES samples. Moreover, because we could not detect specific chimeric genes in samples from non-ESFT, it may be possible to use this technique to diagnose ESFT and to detect tumor cell contamination before hematopoietic stem cell transplantation.</abstract><cop>Tokyo</cop><pub>The Pharmaceutical Society of Japan</pub><pmid>12736494</pmid><doi>10.1248/bpb.26.585</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adolescent Biological and medical sciences Cell Line, Tumor Child Child, Preschool chimeric gene Ewing's sarcoma Female Humans Infant Male Medical sciences Oncogene Proteins, Fusion - genetics Reverse Transcriptase Polymerase Chain Reaction reverse transcription polymerase chain reaction Sarcoma, Ewing - diagnosis Sarcoma, Ewing - genetics Tumor Cells, Cultured |
| title | Diagnostic Significance and Clinical Applications of Chimeric Genes in Ewing's Sarcoma |
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