STRUCTURAL CHARACTERIZATION OF BACITRACIN COMPONENTS BY FRIT-FAST ATOM BOMBARDMENT (FAB) LIQUID CHROMATOGRAPHY/MASS SPECTROMETRY (LC/MS)

The structural characterization of minor components of bacitracin (BC) complex was carried out using a technique of liquid chromatography/mass spectrometry (LC/MS). Satisfactory total ion current chromatogram of BC complex and excellent mass spectra of many components were given by Frit-fast atom bo...

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Veröffentlicht in:Journal of antibiotics 1992/08/25, Vol.45(8), pp.1325-1334
Hauptverfasser: IKAI, YOSHITOMO, OKA, HISAO, HAYAKAWA, JUNKO, HARADA, KEN-ICHI, SUZUKI, MAKOTO
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Sprache:eng
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Zusammenfassung:The structural characterization of minor components of bacitracin (BC) complex was carried out using a technique of liquid chromatography/mass spectrometry (LC/MS). Satisfactory total ion current chromatogram of BC complex and excellent mass spectra of many components were given by Frit-fast atom bombardment (FAB) LC/MS analytical system, and the structures of 13 minor components could be proposed. The 13 minor components were classified into two groups, bacitracin A (BC-A) related components and bacitracin F (BC-F) related components depending on their common N-terminal moieties. The structures of BC-A related components and BC-F related components were the same as those of BC-A and BC-F, respectively, except that one to three of isoleucine and leucine residues are replaced by valines. The BC-F related components were degradation products of BC-A related components through the same degradation process as that of BC-A.
ISSN:0021-8820
1881-1469
DOI:10.7164/antibiotics.45.1325