Contractile Activity of Porcine Neuromedin U-25 and Various Neuromedin U-Related Peptide Fragments on Isolated Chicken Crop Smooth Muscle
Contractile activity of porcine neuromedin U-25 (p-NMU-25) and various neuromedin U (NMU) peptide fragment amides was examined on chicken crop smooth muscle preparation. The relative activity (expressed as RA value) of p-NMU-25 to porcine neuromedin U-8 (p-NMU-8) was 5.51±0.09, and p-NMU-25 (15-25)...
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| Veröffentlicht in: | Chemical & pharmaceutical bulletin 1992/06/25, Vol.40(6), pp.1500-1503 |
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| creator | OKIMURA, Keiko SAKURA, Naoki OHTA, Satoru KUROSAWA, Katsuro HASHIMOTO, Tadashi |
| description | Contractile activity of porcine neuromedin U-25 (p-NMU-25) and various neuromedin U (NMU) peptide fragment amides was examined on chicken crop smooth muscle preparation. The relative activity (expressed as RA value) of p-NMU-25 to porcine neuromedin U-8 (p-NMU-8) was 5.51±0.09, and p-NMU-25 (15-25) was the most potent fragment with an RA value of 7.78±0.05. All C-terminal 11-peptide amides of rat, rabbit and frog NMU peptides retained activity about three-fold higher than the corresponding C-terminal 8-peptide amides. The peptide segment Asn15-Arg-Arg17 of p-NMU-25, as well as the corresponding positions of various NMU peptides : Ser13-Gly-Gly15 of rat NMU and Ser15-Arg-Gly17 of rabbit and frog NMUs, appeared to be involved in the structural requirements for increased contractile activity in the assay system. |
| doi_str_mv | 10.1248/cpb.40.1500 |
| format | Article |
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The relative activity (expressed as RA value) of p-NMU-25 to porcine neuromedin U-8 (p-NMU-8) was 5.51±0.09, and p-NMU-25 (15-25) was the most potent fragment with an RA value of 7.78±0.05. All C-terminal 11-peptide amides of rat, rabbit and frog NMU peptides retained activity about three-fold higher than the corresponding C-terminal 8-peptide amides. The peptide segment Asn15-Arg-Arg17 of p-NMU-25, as well as the corresponding positions of various NMU peptides : Ser13-Gly-Gly15 of rat NMU and Ser15-Arg-Gly17 of rabbit and frog NMUs, appeared to be involved in the structural requirements for increased contractile activity in the assay system.</description><identifier>ISSN: 0009-2363</identifier><identifier>EISSN: 1347-5223</identifier><identifier>DOI: 10.1248/cpb.40.1500</identifier><identifier>PMID: 1394668</identifier><identifier>CODEN: CPBTAL</identifier><language>eng</language><publisher>Tokyo: The Pharmaceutical Society of Japan</publisher><subject>Amino Acid Sequence ; Aminoacids, peptides. Hormones. Neuropeptides ; Analytical, structural and metabolic biochemistry ; Animals ; Biological and medical sciences ; C-terminal undecapeptide amide ; chicken crop ; Chickens ; Crop, Avian - drug effects ; Fundamental and applied biological sciences. Psychology ; In Vitro Techniques ; Molecular Sequence Data ; Muscle, Smooth - drug effects ; neuromedin U ; Neuropeptides - pharmacology ; Peptide Fragments - pharmacology ; porcine ; Proteins ; smooth muscle contraction ; Structure-Activity Relationship ; Swine</subject><ispartof>Chemical and Pharmaceutical Bulletin, 1992/06/25, Vol.40(6), pp.1500-1503</ispartof><rights>The Pharmaceutical Society of Japan</rights><rights>1993 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 1992</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c598t-9adb470156ebd9693344cd4ab68d3a6d13105ebc9b451f0513c72d94416c4bfe3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1877,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4375646$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1394668$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>OKIMURA, Keiko</creatorcontrib><creatorcontrib>SAKURA, Naoki</creatorcontrib><creatorcontrib>OHTA, Satoru</creatorcontrib><creatorcontrib>KUROSAWA, Katsuro</creatorcontrib><creatorcontrib>HASHIMOTO, Tadashi</creatorcontrib><title>Contractile Activity of Porcine Neuromedin U-25 and Various Neuromedin U-Related Peptide Fragments on Isolated Chicken Crop Smooth Muscle</title><title>Chemical & pharmaceutical bulletin</title><addtitle>Chem. Pharm. Bull.</addtitle><description>Contractile activity of porcine neuromedin U-25 (p-NMU-25) and various neuromedin U (NMU) peptide fragment amides was examined on chicken crop smooth muscle preparation. The relative activity (expressed as RA value) of p-NMU-25 to porcine neuromedin U-8 (p-NMU-8) was 5.51±0.09, and p-NMU-25 (15-25) was the most potent fragment with an RA value of 7.78±0.05. All C-terminal 11-peptide amides of rat, rabbit and frog NMU peptides retained activity about three-fold higher than the corresponding C-terminal 8-peptide amides. The peptide segment Asn15-Arg-Arg17 of p-NMU-25, as well as the corresponding positions of various NMU peptides : Ser13-Gly-Gly15 of rat NMU and Ser15-Arg-Gly17 of rabbit and frog NMUs, appeared to be involved in the structural requirements for increased contractile activity in the assay system.</description><subject>Amino Acid Sequence</subject><subject>Aminoacids, peptides. Hormones. Neuropeptides</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>C-terminal undecapeptide amide</subject><subject>chicken crop</subject><subject>Chickens</subject><subject>Crop, Avian - drug effects</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>In Vitro Techniques</subject><subject>Molecular Sequence Data</subject><subject>Muscle, Smooth - drug effects</subject><subject>neuromedin U</subject><subject>Neuropeptides - pharmacology</subject><subject>Peptide Fragments - pharmacology</subject><subject>porcine</subject><subject>Proteins</subject><subject>smooth muscle contraction</subject><subject>Structure-Activity Relationship</subject><subject>Swine</subject><issn>0009-2363</issn><issn>1347-5223</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkU9v1DAQxS0EKsvCiTOSJRAXlGLHfxIfq0ChUoEKKNfIcSZdL4kdbAepH4FvjVdZtYKLZ6T308z4PYSeU3JKS16_NXN3ynMvCHmANpTxqhBlyR6iDSFEFSWT7DF6EuOekFKQip2gE8oUl7LeoD-Ndylok-wI-CyX3zbdYj_gKx-MdYA_wxL8BL11-LooBdauxz90sH6J_2pfYdQJenwFc7I94POgbyZwKWLv8EX0q9rsrPkJDjfBz_jb5H3a4U9LNCM8RY8GPUZ4dqxbdH3-_nvzsbj88uGiObssjFB1KpTuO14RKiR0vZKKMc5Nz3Un655p2VNGiYDOqI4LOhBBmanKXnFOpeHdAGyLXq9z5-B_LRBTO9loYBy1g_yptmJlKVWeu0Uv_wP3fgku39ZSLkkpJc8ub9GblTLBxxhgaOdgJx1uW0raQzxtjqfluc_xZPrFcebSZePu2TWPrL866joaPQ5BO2PjHcZZJSSXGXu3YvuY9A3c6Tokm608rKRK1Ie1cn0O2-_lnQ4tOPYXNh2vhw</recordid><startdate>1992</startdate><enddate>1992</enddate><creator>OKIMURA, Keiko</creator><creator>SAKURA, Naoki</creator><creator>OHTA, Satoru</creator><creator>KUROSAWA, Katsuro</creator><creator>HASHIMOTO, Tadashi</creator><general>The Pharmaceutical Society of Japan</general><general>Maruzen</general><general>Japan Science and Technology Agency</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>1992</creationdate><title>Contractile Activity of Porcine Neuromedin U-25 and Various Neuromedin U-Related Peptide Fragments on Isolated Chicken Crop Smooth Muscle</title><author>OKIMURA, Keiko ; SAKURA, Naoki ; OHTA, Satoru ; KUROSAWA, Katsuro ; HASHIMOTO, Tadashi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c598t-9adb470156ebd9693344cd4ab68d3a6d13105ebc9b451f0513c72d94416c4bfe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Amino Acid Sequence</topic><topic>Aminoacids, peptides. Hormones. Neuropeptides</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>C-terminal undecapeptide amide</topic><topic>chicken crop</topic><topic>Chickens</topic><topic>Crop, Avian - drug effects</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>In Vitro Techniques</topic><topic>Molecular Sequence Data</topic><topic>Muscle, Smooth - drug effects</topic><topic>neuromedin U</topic><topic>Neuropeptides - pharmacology</topic><topic>Peptide Fragments - pharmacology</topic><topic>porcine</topic><topic>Proteins</topic><topic>smooth muscle contraction</topic><topic>Structure-Activity Relationship</topic><topic>Swine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>OKIMURA, Keiko</creatorcontrib><creatorcontrib>SAKURA, Naoki</creatorcontrib><creatorcontrib>OHTA, Satoru</creatorcontrib><creatorcontrib>KUROSAWA, Katsuro</creatorcontrib><creatorcontrib>HASHIMOTO, Tadashi</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Chemical & pharmaceutical bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>OKIMURA, Keiko</au><au>SAKURA, Naoki</au><au>OHTA, Satoru</au><au>KUROSAWA, Katsuro</au><au>HASHIMOTO, Tadashi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Contractile Activity of Porcine Neuromedin U-25 and Various Neuromedin U-Related Peptide Fragments on Isolated Chicken Crop Smooth Muscle</atitle><jtitle>Chemical & pharmaceutical bulletin</jtitle><addtitle>Chem. Pharm. Bull.</addtitle><date>1992</date><risdate>1992</risdate><volume>40</volume><issue>6</issue><spage>1500</spage><epage>1503</epage><pages>1500-1503</pages><issn>0009-2363</issn><eissn>1347-5223</eissn><coden>CPBTAL</coden><abstract>Contractile activity of porcine neuromedin U-25 (p-NMU-25) and various neuromedin U (NMU) peptide fragment amides was examined on chicken crop smooth muscle preparation. The relative activity (expressed as RA value) of p-NMU-25 to porcine neuromedin U-8 (p-NMU-8) was 5.51±0.09, and p-NMU-25 (15-25) was the most potent fragment with an RA value of 7.78±0.05. All C-terminal 11-peptide amides of rat, rabbit and frog NMU peptides retained activity about three-fold higher than the corresponding C-terminal 8-peptide amides. The peptide segment Asn15-Arg-Arg17 of p-NMU-25, as well as the corresponding positions of various NMU peptides : Ser13-Gly-Gly15 of rat NMU and Ser15-Arg-Gly17 of rabbit and frog NMUs, appeared to be involved in the structural requirements for increased contractile activity in the assay system.</abstract><cop>Tokyo</cop><pub>The Pharmaceutical Society of Japan</pub><pmid>1394668</pmid><doi>10.1248/cpb.40.1500</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Chemical and Pharmaceutical Bulletin, 1992/06/25, Vol.40(6), pp.1500-1503 |
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| subjects | Amino Acid Sequence Aminoacids, peptides. Hormones. Neuropeptides Analytical, structural and metabolic biochemistry Animals Biological and medical sciences C-terminal undecapeptide amide chicken crop Chickens Crop, Avian - drug effects Fundamental and applied biological sciences. Psychology In Vitro Techniques Molecular Sequence Data Muscle, Smooth - drug effects neuromedin U Neuropeptides - pharmacology Peptide Fragments - pharmacology porcine Proteins smooth muscle contraction Structure-Activity Relationship Swine |
| title | Contractile Activity of Porcine Neuromedin U-25 and Various Neuromedin U-Related Peptide Fragments on Isolated Chicken Crop Smooth Muscle |
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