Fast kinetics studies of Escherichia coli electrotransformation

Direct gene transfer is achieved in Escherichia coli by use of square wave electric pulsing. As observed by video monitoring, the field pulse causes bacteria to orientate parallel to the field lines. Rapid kinetic turbidity changes indicate that this process happens quickly. In these circumstances,...

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Veröffentlicht in:	European journal of biochemistry 1992-10, Vol.209 (1), p.431-436
Hauptverfasser:	EYNARD, Nathalie, SIXOU, Sophie, DURAN, Nathalie, TEISSIE, Justin
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Triphosphate - metabolism Buffers Cell Membrane Permeability Cytoplasm - metabolism Electricity Escherichia coli - genetics Kinetics Osmolar Concentration Osmotic Pressure Periodicity Plasmids Transfection Transformation, Bacterial
Online-Zugang:	Volltext
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