The combined EEG-intracerebral microdialysis technique: a new tool for neuropharmacological studies on freely behaving animals
In this study we combined EEG and intracerebral microdialysis techniques in freely behaving rats. Various drugs were delivered into the hippocampus and cerebral cortex by means of microdialysis and, simultaneously, the EEG activity of the dialyzed area was monitored. The microdialysis procedure itse...
Gespeichert in:
| Veröffentlicht in: | Journal of neuroscience methods 1992-07, Vol.43 (2), p.129-137 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 137 |
|---|---|
| container_issue | 2 |
| container_start_page | 129 |
| container_title | Journal of neuroscience methods |
| container_volume | 43 |
| creator | Ludvig, N. Mishra, P.K. Yan, Q.S. Lasley, S.M. Burger, R.L. Jobe, P.C. |
| description | In this study we combined EEG and intracerebral microdialysis techniques in freely behaving rats. Various drugs were delivered into the hippocampus and cerebral cortex by means of microdialysis and, simultaneously, the EEG activity of the dialyzed area was monitored. The microdialysis procedure itself, when artificial cerebrospinal fluid was perfused, did not change the normal hippocampal or cortical EEG pattern. Drug inclusions into the microdialysis fluid, however, caused marked changes in the electrical activity of the dialyzed sites. In this report we present the following examples: (1) the dose-dependent spike-provoking effect of NMDA in hippocampus, (2) the potentiation of this NMDA effect in hippocampus by dibutyryl cyclic AMP, and (3) the EEG depressant effect of high concentration of K
+ in the cerebral cortex. The artificial cerebrospinal fluid and drug solutions were alternated in the microdialysis system with a 2-way valve placed outside the test chamber. As a consequence, the drugs were delivered into the brain without interrupting the ongoing behavior, including sleep, of the examined animals. This study shows that the combined EEG-intracerebral microdialysis technique is a useful tool, with many unique advantages, for in vivo neuropharmacological studies. |
| doi_str_mv | 10.1016/0165-0270(92)90022-6 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_73222637</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0165027092900226</els_id><sourcerecordid>16508550</sourcerecordid><originalsourceid>FETCH-LOGICAL-c417t-d0fcf0fb0893da65489b7af2cfdf500c78c7c282ccdf445d2bcc3bf09334e4723</originalsourceid><addsrcrecordid>eNqFkU9v1DAQxS1EVZbCNwDJB4TgkDJx_jjpAQlVS1upEpcicbOc8bhrlNiLnRTthc-Ol12VGxyssTVvRs-_x9irEs5LKNsP-TQFCAnvevG-BxCiaJ-wVdnJfJHdt6ds9Sh5xp6n9B0A6h7aU3ZaVqKTUqzYr7sNcQzT4DwZvl5fFc7PUSNFGqIe-eQwBuP0uEsu8Zlw492PhS645p5-8jmEkdsQ82OJYbvRcdIYxnDvMA-neTGOEg-e20g07vhAG_3g_D3X3k16TC_Yic2FXh7rGfv6eX13eV3cfrm6ufx0W2BdyrkwYNGCHaDrK6Pbpu76QWor0BrbAKDsUKLoBKKxdd0YMSBWg4W-qmqqpajO2NvD3m0M2X6a1eQS0jhqT2FJSlZCiLaS_xVmoNA1DWRhfRBmPilFsmob85fiTpWg9vmoPXy1h696of7ko9o89vq4fxkmMn-HDoHk_ptjX6eM0Ebt0aVHWV31omn2Nj8eZJShPTiKKqEjj2RcJJyVCe7fPn4Dov6uiA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16508550</pqid></control><display><type>article</type><title>The combined EEG-intracerebral microdialysis technique: a new tool for neuropharmacological studies on freely behaving animals</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><creator>Ludvig, N. ; Mishra, P.K. ; Yan, Q.S. ; Lasley, S.M. ; Burger, R.L. ; Jobe, P.C.</creator><creatorcontrib>Ludvig, N. ; Mishra, P.K. ; Yan, Q.S. ; Lasley, S.M. ; Burger, R.L. ; Jobe, P.C.</creatorcontrib><description>In this study we combined EEG and intracerebral microdialysis techniques in freely behaving rats. Various drugs were delivered into the hippocampus and cerebral cortex by means of microdialysis and, simultaneously, the EEG activity of the dialyzed area was monitored. The microdialysis procedure itself, when artificial cerebrospinal fluid was perfused, did not change the normal hippocampal or cortical EEG pattern. Drug inclusions into the microdialysis fluid, however, caused marked changes in the electrical activity of the dialyzed sites. In this report we present the following examples: (1) the dose-dependent spike-provoking effect of NMDA in hippocampus, (2) the potentiation of this NMDA effect in hippocampus by dibutyryl cyclic AMP, and (3) the EEG depressant effect of high concentration of K
+ in the cerebral cortex. The artificial cerebrospinal fluid and drug solutions were alternated in the microdialysis system with a 2-way valve placed outside the test chamber. As a consequence, the drugs were delivered into the brain without interrupting the ongoing behavior, including sleep, of the examined animals. This study shows that the combined EEG-intracerebral microdialysis technique is a useful tool, with many unique advantages, for in vivo neuropharmacological studies.</description><identifier>ISSN: 0165-0270</identifier><identifier>EISSN: 1872-678X</identifier><identifier>DOI: 10.1016/0165-0270(92)90022-6</identifier><identifier>PMID: 1328772</identifier><identifier>CODEN: JNMEDT</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Behavior, Animal - drug effects ; Biological and medical sciences ; Brain - anatomy & histology ; Brain Chemistry - drug effects ; Bucladesine - pharmacology ; Cerebral Cortex ; Dialysis ; Dibutyryl cyclic AMP ; EEG ; Electrodes ; Electroencephalography ; Female ; Fundamental and applied biological sciences. Psychology ; General aspects. Models. Methods ; Hippocampus ; Injections ; Intracerebral microdialysis ; Male ; N-Methylaspartate - pharmacology ; NMDA ; Rats ; Rats, Sprague-Dawley ; Seizure ; Vertebrates: nervous system and sense organs</subject><ispartof>Journal of neuroscience methods, 1992-07, Vol.43 (2), p.129-137</ispartof><rights>1992</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-d0fcf0fb0893da65489b7af2cfdf500c78c7c282ccdf445d2bcc3bf09334e4723</citedby><cites>FETCH-LOGICAL-c417t-d0fcf0fb0893da65489b7af2cfdf500c78c7c282ccdf445d2bcc3bf09334e4723</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0165-0270(92)90022-6$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4392557$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1328772$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ludvig, N.</creatorcontrib><creatorcontrib>Mishra, P.K.</creatorcontrib><creatorcontrib>Yan, Q.S.</creatorcontrib><creatorcontrib>Lasley, S.M.</creatorcontrib><creatorcontrib>Burger, R.L.</creatorcontrib><creatorcontrib>Jobe, P.C.</creatorcontrib><title>The combined EEG-intracerebral microdialysis technique: a new tool for neuropharmacological studies on freely behaving animals</title><title>Journal of neuroscience methods</title><addtitle>J Neurosci Methods</addtitle><description>In this study we combined EEG and intracerebral microdialysis techniques in freely behaving rats. Various drugs were delivered into the hippocampus and cerebral cortex by means of microdialysis and, simultaneously, the EEG activity of the dialyzed area was monitored. The microdialysis procedure itself, when artificial cerebrospinal fluid was perfused, did not change the normal hippocampal or cortical EEG pattern. Drug inclusions into the microdialysis fluid, however, caused marked changes in the electrical activity of the dialyzed sites. In this report we present the following examples: (1) the dose-dependent spike-provoking effect of NMDA in hippocampus, (2) the potentiation of this NMDA effect in hippocampus by dibutyryl cyclic AMP, and (3) the EEG depressant effect of high concentration of K
+ in the cerebral cortex. The artificial cerebrospinal fluid and drug solutions were alternated in the microdialysis system with a 2-way valve placed outside the test chamber. As a consequence, the drugs were delivered into the brain without interrupting the ongoing behavior, including sleep, of the examined animals. This study shows that the combined EEG-intracerebral microdialysis technique is a useful tool, with many unique advantages, for in vivo neuropharmacological studies.</description><subject>Animals</subject><subject>Behavior, Animal - drug effects</subject><subject>Biological and medical sciences</subject><subject>Brain - anatomy & histology</subject><subject>Brain Chemistry - drug effects</subject><subject>Bucladesine - pharmacology</subject><subject>Cerebral Cortex</subject><subject>Dialysis</subject><subject>Dibutyryl cyclic AMP</subject><subject>EEG</subject><subject>Electrodes</subject><subject>Electroencephalography</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects. Models. Methods</subject><subject>Hippocampus</subject><subject>Injections</subject><subject>Intracerebral microdialysis</subject><subject>Male</subject><subject>N-Methylaspartate - pharmacology</subject><subject>NMDA</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Seizure</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0165-0270</issn><issn>1872-678X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9v1DAQxS1EVZbCNwDJB4TgkDJx_jjpAQlVS1upEpcicbOc8bhrlNiLnRTthc-Ol12VGxyssTVvRs-_x9irEs5LKNsP-TQFCAnvevG-BxCiaJ-wVdnJfJHdt6ds9Sh5xp6n9B0A6h7aU3ZaVqKTUqzYr7sNcQzT4DwZvl5fFc7PUSNFGqIe-eQwBuP0uEsu8Zlw492PhS645p5-8jmEkdsQ82OJYbvRcdIYxnDvMA-neTGOEg-e20g07vhAG_3g_D3X3k16TC_Yic2FXh7rGfv6eX13eV3cfrm6ufx0W2BdyrkwYNGCHaDrK6Pbpu76QWor0BrbAKDsUKLoBKKxdd0YMSBWg4W-qmqqpajO2NvD3m0M2X6a1eQS0jhqT2FJSlZCiLaS_xVmoNA1DWRhfRBmPilFsmob85fiTpWg9vmoPXy1h696of7ko9o89vq4fxkmMn-HDoHk_ptjX6eM0Ebt0aVHWV31omn2Nj8eZJShPTiKKqEjj2RcJJyVCe7fPn4Dov6uiA</recordid><startdate>19920701</startdate><enddate>19920701</enddate><creator>Ludvig, N.</creator><creator>Mishra, P.K.</creator><creator>Yan, Q.S.</creator><creator>Lasley, S.M.</creator><creator>Burger, R.L.</creator><creator>Jobe, P.C.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>19920701</creationdate><title>The combined EEG-intracerebral microdialysis technique: a new tool for neuropharmacological studies on freely behaving animals</title><author>Ludvig, N. ; Mishra, P.K. ; Yan, Q.S. ; Lasley, S.M. ; Burger, R.L. ; Jobe, P.C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-d0fcf0fb0893da65489b7af2cfdf500c78c7c282ccdf445d2bcc3bf09334e4723</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Animals</topic><topic>Behavior, Animal - drug effects</topic><topic>Biological and medical sciences</topic><topic>Brain - anatomy & histology</topic><topic>Brain Chemistry - drug effects</topic><topic>Bucladesine - pharmacology</topic><topic>Cerebral Cortex</topic><topic>Dialysis</topic><topic>Dibutyryl cyclic AMP</topic><topic>EEG</topic><topic>Electrodes</topic><topic>Electroencephalography</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects. Models. Methods</topic><topic>Hippocampus</topic><topic>Injections</topic><topic>Intracerebral microdialysis</topic><topic>Male</topic><topic>N-Methylaspartate - pharmacology</topic><topic>NMDA</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Seizure</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ludvig, N.</creatorcontrib><creatorcontrib>Mishra, P.K.</creatorcontrib><creatorcontrib>Yan, Q.S.</creatorcontrib><creatorcontrib>Lasley, S.M.</creatorcontrib><creatorcontrib>Burger, R.L.</creatorcontrib><creatorcontrib>Jobe, P.C.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neuroscience methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ludvig, N.</au><au>Mishra, P.K.</au><au>Yan, Q.S.</au><au>Lasley, S.M.</au><au>Burger, R.L.</au><au>Jobe, P.C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The combined EEG-intracerebral microdialysis technique: a new tool for neuropharmacological studies on freely behaving animals</atitle><jtitle>Journal of neuroscience methods</jtitle><addtitle>J Neurosci Methods</addtitle><date>1992-07-01</date><risdate>1992</risdate><volume>43</volume><issue>2</issue><spage>129</spage><epage>137</epage><pages>129-137</pages><issn>0165-0270</issn><eissn>1872-678X</eissn><coden>JNMEDT</coden><abstract>In this study we combined EEG and intracerebral microdialysis techniques in freely behaving rats. Various drugs were delivered into the hippocampus and cerebral cortex by means of microdialysis and, simultaneously, the EEG activity of the dialyzed area was monitored. The microdialysis procedure itself, when artificial cerebrospinal fluid was perfused, did not change the normal hippocampal or cortical EEG pattern. Drug inclusions into the microdialysis fluid, however, caused marked changes in the electrical activity of the dialyzed sites. In this report we present the following examples: (1) the dose-dependent spike-provoking effect of NMDA in hippocampus, (2) the potentiation of this NMDA effect in hippocampus by dibutyryl cyclic AMP, and (3) the EEG depressant effect of high concentration of K
+ in the cerebral cortex. The artificial cerebrospinal fluid and drug solutions were alternated in the microdialysis system with a 2-way valve placed outside the test chamber. As a consequence, the drugs were delivered into the brain without interrupting the ongoing behavior, including sleep, of the examined animals. This study shows that the combined EEG-intracerebral microdialysis technique is a useful tool, with many unique advantages, for in vivo neuropharmacological studies.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>1328772</pmid><doi>10.1016/0165-0270(92)90022-6</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0165-0270 |
| ispartof | Journal of neuroscience methods, 1992-07, Vol.43 (2), p.129-137 |
| issn | 0165-0270 1872-678X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73222637 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Behavior, Animal - drug effects Biological and medical sciences Brain - anatomy & histology Brain Chemistry - drug effects Bucladesine - pharmacology Cerebral Cortex Dialysis Dibutyryl cyclic AMP EEG Electrodes Electroencephalography Female Fundamental and applied biological sciences. Psychology General aspects. Models. Methods Hippocampus Injections Intracerebral microdialysis Male N-Methylaspartate - pharmacology NMDA Rats Rats, Sprague-Dawley Seizure Vertebrates: nervous system and sense organs |
| title | The combined EEG-intracerebral microdialysis technique: a new tool for neuropharmacological studies on freely behaving animals |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-05T02%3A44%3A11IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20combined%20EEG-intracerebral%20microdialysis%20technique:%20a%20new%20tool%20for%20neuropharmacological%20studies%20on%20freely%20behaving%20animals&rft.jtitle=Journal%20of%20neuroscience%20methods&rft.au=Ludvig,%20N.&rft.date=1992-07-01&rft.volume=43&rft.issue=2&rft.spage=129&rft.epage=137&rft.pages=129-137&rft.issn=0165-0270&rft.eissn=1872-678X&rft.coden=JNMEDT&rft_id=info:doi/10.1016/0165-0270(92)90022-6&rft_dat=%3Cproquest_cross%3E16508550%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=16508550&rft_id=info:pmid/1328772&rft_els_id=0165027092900226&rfr_iscdi=true |