Suppression of cathepsin B expression in Schistosoma mansoni by RNA interference
In this paper, we used the genetic manipulation technique known as RNA-interference to suppress the expression of a target, cathepsin B, gene in the platyhelminth parasite, Schistosoma mansoni. Parasites were cultured for 6 days in the presence of double stranded RNA derived from the cathepsin B cDN...
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| Veröffentlicht in: | International journal for parasitology 2003-04, Vol.33 (4), p.363-369 |
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| container_title | International journal for parasitology |
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| creator | Skelly, Patrick J Da'dara, Akram Harn, Donald A |
| description | In this paper, we used the genetic manipulation technique known as RNA-interference to suppress the expression of a target, cathepsin B, gene in the platyhelminth parasite,
Schistosoma mansoni. Parasites were cultured for 6 days in the presence of double stranded RNA derived from the cathepsin B cDNA sequence or from two control sequences. Relative to the controls, the cathepsin B double stranded RNA-treated group exhibited lower levels of cathepsin B as determined by immuno-staining and by enzyme activity measurements. Additionally, using the reverse transcriptase-PCR, suppression was seen in the inability to detect cathepsin B cDNA, using RNA obtained from those parasites. This ability to manipulate gene expression represents a powerful new tool for investigating gene function in these debilitating human parasites. |
| doi_str_mv | 10.1016/S0020-7519(03)00030-4 |
| format | Article |
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Schistosoma mansoni. Parasites were cultured for 6 days in the presence of double stranded RNA derived from the cathepsin B cDNA sequence or from two control sequences. Relative to the controls, the cathepsin B double stranded RNA-treated group exhibited lower levels of cathepsin B as determined by immuno-staining and by enzyme activity measurements. Additionally, using the reverse transcriptase-PCR, suppression was seen in the inability to detect cathepsin B cDNA, using RNA obtained from those parasites. This ability to manipulate gene expression represents a powerful new tool for investigating gene function in these debilitating human parasites.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Cathepsin B</subject><subject>Cathepsin B - genetics</subject><subject>Cathepsin B - metabolism</subject><subject>DNA, Complementary - genetics</subject><subject>DNA, Helminth - genetics</subject><subject>DNA, Helminth - metabolism</subject><subject>Immunohistochemistry</subject><subject>Molecular Sequence Data</subject><subject>Parasites</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA Interference - physiology</subject><subject>RNA, Helminth - genetics</subject><subject>RNA, Helminth - metabolism</subject><subject>RNAi</subject><subject>Schistosoma mansoni</subject><subject>Schistosoma mansoni - enzymology</subject><subject>Schistosoma mansoni - genetics</subject><issn>0020-7519</issn><issn>1879-0135</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1LJDEQhoO46PjxE5Q-iR56t_LVmT7JKH4syCqOnkOSrmBkutMmPYv---1xBvfoqSje562Ch5AjCj8p0OrXHIBBqSStT4GfAQCHUmyRCZ2qugTK5TaZfCG7ZC_nVwAquRA7ZJcyBbLmMCEP82XfJ8w5xK6IvnBmeME-h664KPD9Kxn3uXsJeYg5tqZoTZdjFwr7UTz-mY3pgMljws7hAfnhzSLj4Wbuk-frq6fL2_Lu_ub35eyudEJOh9LWQjIluAElUVqjuKyV5awxjXeUcdl4KwAss4rVqqI1o5WilbDeq8Zzy_fJyfpun-LbEvOg25AdLhamw7jMWvGxMZXsW5CBqnjFYATlGnQp5pzQ6z6F1qQPTUGvnOtP53olVAPXn861GHvHmwdL22Lzv7WRPALnawBHH38DJp1dWLlqQkI36CaGb178A-NTkBI</recordid><startdate>20030401</startdate><enddate>20030401</enddate><creator>Skelly, Patrick J</creator><creator>Da'dara, Akram</creator><creator>Harn, Donald A</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>C1K</scope><scope>F1W</scope><scope>H95</scope><scope>H97</scope><scope>H98</scope><scope>L.G</scope><scope>7X8</scope></search><sort><creationdate>20030401</creationdate><title>Suppression of cathepsin B expression in Schistosoma mansoni by RNA interference</title><author>Skelly, Patrick J ; 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Schistosoma mansoni. Parasites were cultured for 6 days in the presence of double stranded RNA derived from the cathepsin B cDNA sequence or from two control sequences. Relative to the controls, the cathepsin B double stranded RNA-treated group exhibited lower levels of cathepsin B as determined by immuno-staining and by enzyme activity measurements. Additionally, using the reverse transcriptase-PCR, suppression was seen in the inability to detect cathepsin B cDNA, using RNA obtained from those parasites. This ability to manipulate gene expression represents a powerful new tool for investigating gene function in these debilitating human parasites.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>12705930</pmid><doi>10.1016/S0020-7519(03)00030-4</doi><tpages>7</tpages></addata></record> |
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| ispartof | International journal for parasitology, 2003-04, Vol.33 (4), p.363-369 |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Base Sequence Cathepsin B Cathepsin B - genetics Cathepsin B - metabolism DNA, Complementary - genetics DNA, Helminth - genetics DNA, Helminth - metabolism Immunohistochemistry Molecular Sequence Data Parasites Reverse Transcriptase Polymerase Chain Reaction RNA Interference - physiology RNA, Helminth - genetics RNA, Helminth - metabolism RNAi Schistosoma mansoni Schistosoma mansoni - enzymology Schistosoma mansoni - genetics |
| title | Suppression of cathepsin B expression in Schistosoma mansoni by RNA interference |
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