Spontaneous and stimulated firing in cultured rat suprachiasmatic neurons

Neurons from the suprachiasmatic nucleus (SCN) of the hypothalamus, the site of a circadian pacemaker in mammals, were isolated from embryonic rat. After mechanical dissociation neurons were brought into culture for 1–2 weeks, using a chemically defined medium. Recordings were made from 74 bipolar n...

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Veröffentlicht in:	Brain research 1992-08, Vol.588 (1), p.120-131
Hauptverfasser:	Walsh, I.B., van den Berg, R.J., Marani, E., Rietveld, W.J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Action Potentials - physiology Animals Biological and medical sciences Cells, Cultured Central nervous system Chloride conductance Chlorides - metabolism Cultured neuron Electrophysiology Female Fundamental and applied biological sciences. Psychology Ion Channels - physiology Isoquinolines Membrane Potentials - physiology Neurons - physiology Patch-clamp Pregnancy Rats Rats, Inbred Strains Spontaneous firing Stimulated firing Suprachiasmatic nucleus Suprachiasmatic Nucleus - physiology Vertebrates: nervous system and sense organs
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