Molecular cloning of the gene encoding developing seed L-asparaginase from Lupinus angustifolius
A genomic sequence encoding Lupinus angustifolius L-asparaginase has been obtained, and is the first report of this gene from a plant source. The 3.2 kb of DNA sequenced contains a 1136 bp 5' flanking sequence, four exons and three introns. Intron-exon borders were mapped by comparing the genom...
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| Veröffentlicht in: | Plant molecular biology 1992-10, Vol.20 (2), p.333-336 |
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| container_title | Plant molecular biology |
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| creator | Dickson, J.M.J.J. (Immunology and Plant Molecular Biology Unit DSIR, Palmerston North (New Zealand)) Vincze, E.V Grant, M.R Smith, L.A Rodber, K.A Farnden, K.J.F Reynolds, P.H.S |
| description | A genomic sequence encoding Lupinus angustifolius L-asparaginase has been obtained, and is the first report of this gene from a plant source. The 3.2 kb of DNA sequenced contains a 1136 bp 5' flanking sequence, four exons and three introns. Intron-exon borders were mapped by comparing the genomic sequence with that of a L. arboreus cDNA. Primer extension analysis revealed transcription start sites 16 bp and 13 bp 5' of the initiating ATG for L. angustifolius and L. arboreus, respectively. The 5' flanking region contained sequences associated with seed-specific expression. |
| doi_str_mv | 10.1007/bf00014503 |
| format | Article |
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(Immunology and Plant Molecular Biology Unit DSIR, Palmerston North (New Zealand)) ; Vincze, E.V ; Grant, M.R ; Smith, L.A ; Rodber, K.A ; Farnden, K.J.F ; Reynolds, P.H.S</creator><creatorcontrib>Dickson, J.M.J.J. (Immunology and Plant Molecular Biology Unit DSIR, Palmerston North (New Zealand)) ; Vincze, E.V ; Grant, M.R ; Smith, L.A ; Rodber, K.A ; Farnden, K.J.F ; Reynolds, P.H.S</creatorcontrib><description>A genomic sequence encoding Lupinus angustifolius L-asparaginase has been obtained, and is the first report of this gene from a plant source. The 3.2 kb of DNA sequenced contains a 1136 bp 5' flanking sequence, four exons and three introns. Intron-exon borders were mapped by comparing the genomic sequence with that of a L. arboreus cDNA. Primer extension analysis revealed transcription start sites 16 bp and 13 bp 5' of the initiating ATG for L. angustifolius and L. arboreus, respectively. The 5' flanking region contained sequences associated with seed-specific expression.</description><identifier>ISSN: 0167-4412</identifier><identifier>EISSN: 1573-5028</identifier><identifier>DOI: 10.1007/bf00014503</identifier><identifier>PMID: 1391778</identifier><identifier>CODEN: PMBIDB</identifier><language>eng</language><publisher>Dordrecht: Springer</publisher><subject>ADN ; AMIDE HYDROLASE ; AMIDE HYDROLASES ; AMIDO HIDROLASA ; Amino Acid Sequence ; Asparaginase - genetics ; Base Sequence ; Biological and medical sciences ; CLONACION MOLECULAR ; CLONAGE MOLECULAIRE ; Cloning, Molecular ; CODE GENETIQUE ; CODIGO GENETICO ; DNA ; Fundamental and applied biological sciences. Psychology ; GENETIC CODE ; LUPINUS ANGUSTIFOLIUS ; Molecular and cellular biology ; MOLECULAR CLONING ; Molecular genetics ; Molecular Sequence Data ; NUCLEOTIDE SEQUENCE ; Plants - embryology ; Plants - enzymology ; Plants - genetics ; SECUENCIA NUCLEICA ; Seeds - enzymology ; Seeds - genetics ; Seeds - growth & development ; SEQUENCE NUCLEIQUE</subject><ispartof>Plant molecular biology, 1992-10, Vol.20 (2), p.333-336</ispartof><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c427t-dacb94a1b8355b159c3ae90c7b94d6a36e31e3e6af0b4eca751b3e6850fa06c13</citedby><cites>FETCH-LOGICAL-c427t-dacb94a1b8355b159c3ae90c7b94d6a36e31e3e6af0b4eca751b3e6850fa06c13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4311649$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1391778$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dickson, J.M.J.J. 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Primer extension analysis revealed transcription start sites 16 bp and 13 bp 5' of the initiating ATG for L. angustifolius and L. arboreus, respectively. The 5' flanking region contained sequences associated with seed-specific expression.</description><subject>ADN</subject><subject>AMIDE HYDROLASE</subject><subject>AMIDE HYDROLASES</subject><subject>AMIDO HIDROLASA</subject><subject>Amino Acid Sequence</subject><subject>Asparaginase - genetics</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>CLONACION MOLECULAR</subject><subject>CLONAGE MOLECULAIRE</subject><subject>Cloning, Molecular</subject><subject>CODE GENETIQUE</subject><subject>CODIGO GENETICO</subject><subject>DNA</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GENETIC CODE</subject><subject>LUPINUS ANGUSTIFOLIUS</subject><subject>Molecular and cellular biology</subject><subject>MOLECULAR CLONING</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>NUCLEOTIDE SEQUENCE</subject><subject>Plants - embryology</subject><subject>Plants - enzymology</subject><subject>Plants - genetics</subject><subject>SECUENCIA NUCLEICA</subject><subject>Seeds - enzymology</subject><subject>Seeds - genetics</subject><subject>Seeds - growth & development</subject><subject>SEQUENCE NUCLEIQUE</subject><issn>0167-4412</issn><issn>1573-5028</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0EFv1DAQBWCrArXblkuPSJV8QD0gpXji2E6OpaKAlJYLnMPEGYcgb7zYm0r8-3q1W3rk5PG8T3N4jF2AuAYhzIfeCSGgUkIesRUoIwslyvoVWwnQpqgqKE_YaUq_MxJC6mN2DLIBY-oV-3kfPNnFY-TWh3maRx4c3_4iPtJMnGYbht1yoEfyYbMbE9HA2wLTBiOO04yJuIthzdsl50viOI9L2k4u-GlJ5-y1Q5_ozeE9Yz_uPn2__VK03z5_vb1pC1uVZlsMaPumQuhrqVQPqrESqRHW5O2gUWqSQJI0OtFXZNEo6PO3VsKh0BbkGbva393E8GehtO3WU7LkPc4UltQZWQIo-D8EraGGRmb4fg9tDClFct0mTmuMfzsQ3a737uPdc-8ZXx6uLv2ahhe6Lzrn7w45JoveRZztlP6xSgLoqsns7Z45DB2OMZOHtimFKpWST3Maks0</recordid><startdate>19921001</startdate><enddate>19921001</enddate><creator>Dickson, J.M.J.J. 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(Immunology and Plant Molecular Biology Unit DSIR, Palmerston North (New Zealand)) ; Vincze, E.V ; Grant, M.R ; Smith, L.A ; Rodber, K.A ; Farnden, K.J.F ; Reynolds, P.H.S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c427t-dacb94a1b8355b159c3ae90c7b94d6a36e31e3e6af0b4eca751b3e6850fa06c13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>ADN</topic><topic>AMIDE HYDROLASE</topic><topic>AMIDE HYDROLASES</topic><topic>AMIDO HIDROLASA</topic><topic>Amino Acid Sequence</topic><topic>Asparaginase - genetics</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>CLONACION MOLECULAR</topic><topic>CLONAGE MOLECULAIRE</topic><topic>Cloning, Molecular</topic><topic>CODE GENETIQUE</topic><topic>CODIGO GENETICO</topic><topic>DNA</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GENETIC CODE</topic><topic>LUPINUS ANGUSTIFOLIUS</topic><topic>Molecular and cellular biology</topic><topic>MOLECULAR CLONING</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>NUCLEOTIDE SEQUENCE</topic><topic>Plants - embryology</topic><topic>Plants - enzymology</topic><topic>Plants - genetics</topic><topic>SECUENCIA NUCLEICA</topic><topic>Seeds - enzymology</topic><topic>Seeds - genetics</topic><topic>Seeds - growth & development</topic><topic>SEQUENCE NUCLEIQUE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dickson, J.M.J.J. 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(Immunology and Plant Molecular Biology Unit DSIR, Palmerston North (New Zealand))</au><au>Vincze, E.V</au><au>Grant, M.R</au><au>Smith, L.A</au><au>Rodber, K.A</au><au>Farnden, K.J.F</au><au>Reynolds, P.H.S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular cloning of the gene encoding developing seed L-asparaginase from Lupinus angustifolius</atitle><jtitle>Plant molecular biology</jtitle><addtitle>Plant Mol Biol</addtitle><date>1992-10-01</date><risdate>1992</risdate><volume>20</volume><issue>2</issue><spage>333</spage><epage>336</epage><pages>333-336</pages><issn>0167-4412</issn><eissn>1573-5028</eissn><coden>PMBIDB</coden><abstract>A genomic sequence encoding Lupinus angustifolius L-asparaginase has been obtained, and is the first report of this gene from a plant source. The 3.2 kb of DNA sequenced contains a 1136 bp 5' flanking sequence, four exons and three introns. Intron-exon borders were mapped by comparing the genomic sequence with that of a L. arboreus cDNA. Primer extension analysis revealed transcription start sites 16 bp and 13 bp 5' of the initiating ATG for L. angustifolius and L. arboreus, respectively. The 5' flanking region contained sequences associated with seed-specific expression.</abstract><cop>Dordrecht</cop><pub>Springer</pub><pmid>1391778</pmid><doi>10.1007/bf00014503</doi><tpages>4</tpages></addata></record> |
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| subjects | ADN AMIDE HYDROLASE AMIDE HYDROLASES AMIDO HIDROLASA Amino Acid Sequence Asparaginase - genetics Base Sequence Biological and medical sciences CLONACION MOLECULAR CLONAGE MOLECULAIRE Cloning, Molecular CODE GENETIQUE CODIGO GENETICO DNA Fundamental and applied biological sciences. Psychology GENETIC CODE LUPINUS ANGUSTIFOLIUS Molecular and cellular biology MOLECULAR CLONING Molecular genetics Molecular Sequence Data NUCLEOTIDE SEQUENCE Plants - embryology Plants - enzymology Plants - genetics SECUENCIA NUCLEICA Seeds - enzymology Seeds - genetics Seeds - growth & development SEQUENCE NUCLEIQUE |
| title | Molecular cloning of the gene encoding developing seed L-asparaginase from Lupinus angustifolius |
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