Killing of Caenorhabditis elegans by Burkholderia cepacia is controlled by the cep quorum‐sensing system
Summary Burkholderia cepacia H111, which was isolated from a cystic fibrosis patient, effectively kills the nematode Caenorhabditis elegans. Depending on the medium used for growth of the bacterium two different killing modes were observed. On high‐osmolarity medium the nematodes became paralysed an...
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| Veröffentlicht in: | Cellular microbiology 2003-05, Vol.5 (5), p.343-351 |
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| creator | Köthe, Manuela Antl, Melanie Huber, Birgit Stoecker, Kilian Ebrecht, Doreen Steinmetz, Ivo Eberl, Leo |
| description | Summary
Burkholderia cepacia H111, which was isolated from a cystic fibrosis patient, effectively kills the nematode Caenorhabditis elegans. Depending on the medium used for growth of the bacterium two different killing modes were observed. On high‐osmolarity medium the nematodes became paralysed and died within 24 h. Using filter assays we provide evidence that this killing mode involves the production of an extracellular toxin. On nematode growth medium killing occurs over the course of 2–3 days and involves the accumulation of bacteria in the intestinal lumen of C. elegans. We demonstrate that the cep quorum‐sensing system of H111 is required for efficient killing of C. elegans under both killing conditions. Using the C. elegans phm‐2 mutant that has a non‐functional grinder evidence is provided that the cep system is required to enter the intestinal lumen but is dispensable for the colonization of the gut. Furthermore, we demonstrate that the type II secretion machinery is not essential for nematode killing. |
| doi_str_mv | 10.1046/j.1462-5822.2003.00280.x |
| format | Article |
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Burkholderia cepacia H111, which was isolated from a cystic fibrosis patient, effectively kills the nematode Caenorhabditis elegans. Depending on the medium used for growth of the bacterium two different killing modes were observed. On high‐osmolarity medium the nematodes became paralysed and died within 24 h. Using filter assays we provide evidence that this killing mode involves the production of an extracellular toxin. On nematode growth medium killing occurs over the course of 2–3 days and involves the accumulation of bacteria in the intestinal lumen of C. elegans. We demonstrate that the cep quorum‐sensing system of H111 is required for efficient killing of C. elegans under both killing conditions. Using the C. elegans phm‐2 mutant that has a non‐functional grinder evidence is provided that the cep system is required to enter the intestinal lumen but is dispensable for the colonization of the gut. Furthermore, we demonstrate that the type II secretion machinery is not essential for nematode killing.</description><identifier>ISSN: 1462-5814</identifier><identifier>EISSN: 1462-5822</identifier><identifier>DOI: 10.1046/j.1462-5822.2003.00280.x</identifier><identifier>PMID: 12713492</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>Animals ; Bacterial Proteins - genetics ; Bacterial Proteins - physiology ; Burkholderia cepacia - genetics ; Burkholderia cepacia - pathogenicity ; Burkholderia cepacia - physiology ; Caenorhabditis elegans - genetics ; Caenorhabditis elegans - microbiology ; Caenorhabditis elegans Proteins - genetics ; Genes, Bacterial ; Genes, Helminth ; Intestines - microbiology ; Mutation ; Virulence - genetics ; Virulence - physiology</subject><ispartof>Cellular microbiology, 2003-05, Vol.5 (5), p.343-351</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3970-653009f5b22201049d6caeb5c88f0f24d8a2a3da69e6d90fd0d60162b57345033</citedby><cites>FETCH-LOGICAL-c3970-653009f5b22201049d6caeb5c88f0f24d8a2a3da69e6d90fd0d60162b57345033</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1462-5822.2003.00280.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1462-5822.2003.00280.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27923,27924,45573,45574,46408,46832</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12713492$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Köthe, Manuela</creatorcontrib><creatorcontrib>Antl, Melanie</creatorcontrib><creatorcontrib>Huber, Birgit</creatorcontrib><creatorcontrib>Stoecker, Kilian</creatorcontrib><creatorcontrib>Ebrecht, Doreen</creatorcontrib><creatorcontrib>Steinmetz, Ivo</creatorcontrib><creatorcontrib>Eberl, Leo</creatorcontrib><title>Killing of Caenorhabditis elegans by Burkholderia cepacia is controlled by the cep quorum‐sensing system</title><title>Cellular microbiology</title><addtitle>Cell Microbiol</addtitle><description>Summary
Burkholderia cepacia H111, which was isolated from a cystic fibrosis patient, effectively kills the nematode Caenorhabditis elegans. Depending on the medium used for growth of the bacterium two different killing modes were observed. On high‐osmolarity medium the nematodes became paralysed and died within 24 h. Using filter assays we provide evidence that this killing mode involves the production of an extracellular toxin. On nematode growth medium killing occurs over the course of 2–3 days and involves the accumulation of bacteria in the intestinal lumen of C. elegans. We demonstrate that the cep quorum‐sensing system of H111 is required for efficient killing of C. elegans under both killing conditions. Using the C. elegans phm‐2 mutant that has a non‐functional grinder evidence is provided that the cep system is required to enter the intestinal lumen but is dispensable for the colonization of the gut. Furthermore, we demonstrate that the type II secretion machinery is not essential for nematode killing.</description><subject>Animals</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - physiology</subject><subject>Burkholderia cepacia - genetics</subject><subject>Burkholderia cepacia - pathogenicity</subject><subject>Burkholderia cepacia - physiology</subject><subject>Caenorhabditis elegans - genetics</subject><subject>Caenorhabditis elegans - microbiology</subject><subject>Caenorhabditis elegans Proteins - genetics</subject><subject>Genes, Bacterial</subject><subject>Genes, Helminth</subject><subject>Intestines - microbiology</subject><subject>Mutation</subject><subject>Virulence - genetics</subject><subject>Virulence - physiology</subject><issn>1462-5814</issn><issn>1462-5822</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1OwzAQhS0E4qdwBZQVu4axnTiJxAYq_gSIDawtJ560KU5c7ES0O47AGTkJCa1gCasZab55ozePkIBCSCESp_OQRoKN45SxkAHwEIClEC63yP7PYPunp9EeOfB-DkBFQuku2aMsoTzK2D6Z31XGVM00sGUwUdhYN1O5rtrKB2hwqhof5KvgonMvM2s0ukoFBS5U0dceKWzTOmsM6oFqZzgMg9fOuq7-fP_w2PhB2698i_Uh2SmV8Xi0qSPyfHX5NLkZ3z9e307O78cFzxIYi5gDZGWcM8agd5tpUSjM4yJNSyhZpFPFFNdKZCh0BqUGLXpjLI8THsXA-YicrHUXzr526FtZV75AY1SDtvMy4YzSiIo_QZqmgnE6KKZrsHDWe4elXLiqVm4lKcghEDmXw6_l8Hc5BCK_A5HLfvV4c6PLa9S_i5sEeuBsDbxVBlf_FpaTh9u-4V_LCZp5</recordid><startdate>200305</startdate><enddate>200305</enddate><creator>Köthe, Manuela</creator><creator>Antl, Melanie</creator><creator>Huber, Birgit</creator><creator>Stoecker, Kilian</creator><creator>Ebrecht, Doreen</creator><creator>Steinmetz, Ivo</creator><creator>Eberl, Leo</creator><general>Blackwell Science Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>200305</creationdate><title>Killing of Caenorhabditis elegans by Burkholderia cepacia is controlled by the cep quorum‐sensing system</title><author>Köthe, Manuela ; Antl, Melanie ; Huber, Birgit ; Stoecker, Kilian ; Ebrecht, Doreen ; Steinmetz, Ivo ; Eberl, Leo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3970-653009f5b22201049d6caeb5c88f0f24d8a2a3da69e6d90fd0d60162b57345033</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - physiology</topic><topic>Burkholderia cepacia - genetics</topic><topic>Burkholderia cepacia - pathogenicity</topic><topic>Burkholderia cepacia - physiology</topic><topic>Caenorhabditis elegans - genetics</topic><topic>Caenorhabditis elegans - microbiology</topic><topic>Caenorhabditis elegans Proteins - genetics</topic><topic>Genes, Bacterial</topic><topic>Genes, Helminth</topic><topic>Intestines - microbiology</topic><topic>Mutation</topic><topic>Virulence - genetics</topic><topic>Virulence - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Köthe, Manuela</creatorcontrib><creatorcontrib>Antl, Melanie</creatorcontrib><creatorcontrib>Huber, Birgit</creatorcontrib><creatorcontrib>Stoecker, Kilian</creatorcontrib><creatorcontrib>Ebrecht, Doreen</creatorcontrib><creatorcontrib>Steinmetz, Ivo</creatorcontrib><creatorcontrib>Eberl, Leo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Cellular microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Köthe, Manuela</au><au>Antl, Melanie</au><au>Huber, Birgit</au><au>Stoecker, Kilian</au><au>Ebrecht, Doreen</au><au>Steinmetz, Ivo</au><au>Eberl, Leo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Killing of Caenorhabditis elegans by Burkholderia cepacia is controlled by the cep quorum‐sensing system</atitle><jtitle>Cellular microbiology</jtitle><addtitle>Cell Microbiol</addtitle><date>2003-05</date><risdate>2003</risdate><volume>5</volume><issue>5</issue><spage>343</spage><epage>351</epage><pages>343-351</pages><issn>1462-5814</issn><eissn>1462-5822</eissn><abstract>Summary
Burkholderia cepacia H111, which was isolated from a cystic fibrosis patient, effectively kills the nematode Caenorhabditis elegans. Depending on the medium used for growth of the bacterium two different killing modes were observed. On high‐osmolarity medium the nematodes became paralysed and died within 24 h. Using filter assays we provide evidence that this killing mode involves the production of an extracellular toxin. On nematode growth medium killing occurs over the course of 2–3 days and involves the accumulation of bacteria in the intestinal lumen of C. elegans. We demonstrate that the cep quorum‐sensing system of H111 is required for efficient killing of C. elegans under both killing conditions. Using the C. elegans phm‐2 mutant that has a non‐functional grinder evidence is provided that the cep system is required to enter the intestinal lumen but is dispensable for the colonization of the gut. Furthermore, we demonstrate that the type II secretion machinery is not essential for nematode killing.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>12713492</pmid><doi>10.1046/j.1462-5822.2003.00280.x</doi><tpages>9</tpages></addata></record> |
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| source | MEDLINE; Wiley Free Content; EZB-FREE-00999 freely available EZB journals; Wiley Online Library All Journals; Alma/SFX Local Collection |
| subjects | Animals Bacterial Proteins - genetics Bacterial Proteins - physiology Burkholderia cepacia - genetics Burkholderia cepacia - pathogenicity Burkholderia cepacia - physiology Caenorhabditis elegans - genetics Caenorhabditis elegans - microbiology Caenorhabditis elegans Proteins - genetics Genes, Bacterial Genes, Helminth Intestines - microbiology Mutation Virulence - genetics Virulence - physiology |
| title | Killing of Caenorhabditis elegans by Burkholderia cepacia is controlled by the cep quorum‐sensing system |
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